Cone snail peptides

ABSTRACT

The present invention is directed to conotoxin peptides, derivatives or pharmaceutically acceptable salts thereof. The present invention is further directed to the use of this peptide, derivatives thereof and pharmaceutically acceptable salts thereof for the treatment of disorders associated with voltage-gated ion channels, voltage-gated ligand channels and/or receptors. The invention is further directed to nucleic acid sequences encoding the conotoxin peptides and encoding propeptides, as well as the propeptides.

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] The present application is related to and claims priority under35 USC §119(e) to U.S. provisional patent application Serial No.60/267,408 filed Feb. 9, 2001, incorporated herein by reference.

[0002] This invention was made with Government support under Grant No.PO1 GM48677 awarded by the National Institute of General MedicalSciences, National Institutes of Health, Bethesda, Md. The United StatesGovernment has certain rights in the invention.

BACKGROUND OF THE INVENTION

[0003] The present invention is directed to conotoxin peptides,derivatives or pharmaceutically acceptable salts thereof. The presentinvention is further directed to the use of this peptide, derivativesthereof and pharmaceutically acceptable salts thereof for the treatmentof disorders associated with voltage-gated ion channels, ligand-gatedion channels and/or receptors. The invention is further directed tonucleic acid sequences encoding the conotoxin peptides and encodingpropeptides, as well as the propeptides.

[0004] The publications and other materials used herein to illuminatethe background of the invention, and in particular, cases to provideadditional details respecting the practice, are incorporated byreference, and for convenience are referenced in the following text byauthor and date and are listed alphabetically by author in the appendedbibliography.

[0005] Conus is a genus of predatory marine gastropods (snails) whichenvenomate their prey. Venomous cone snails use a highly developedprojectile apparatus to deliver their cocktail of toxic conotoxins intotheir prey. In fish-eating species such as Conus magus the cone detectsthe presence of the fish using chemosensors in its siphon and when closeenough extends its proboscis and fires a hollow harpoon-like toothcontaining venom into the fish. This immobilizes the fish and enablesthe cone snail to wind it into its mouth via an attached filament. Forgeneral information on Conus and their venom see the website addresshttp://grimwade.biochem.unimelb.edu.au/cone/referenc.html. Prey captureis accomplished through a sophisticated arsenal of peptides which targetspecific ion channel and receptor subtypes. Each Conus species venomappears to contain a unique set of 50-200 peptides. The composition ofthe venom differs greatly between species and between individual snailswithin each species, each optimally evolved to paralyse it's prey. Theactive components of the venom are small peptides toxins, typically12-30 amino acid residues in length and are typically highly constrainedpeptides due to their high density of disulphide bonds.

[0006] The venoms consist of a large number of different peptidecomponents that when separated exhibit a range of biological activities:when injected into mice they elicit a range of physiological responsesfrom shaking to depression. The paralytic components of the venom thathave been the focus of recent investigation are the α-, ω- andμ-conotoxins. All of these conotoxins act by preventing neuronalcommunication, but each targets a different aspect of the process toachieve this. The α-conotoxins target nicotinic ligand gated channels,the μ-conotoxins target the voltage-gated sodium channels and theω-conotoxins target the voltage-gated calcium channels (Olivera et al.,1985; Olivera et al., 1990). For example a linkage has been establishedbetween α-, αA- & φ-conotoxins and the nicotinic ligand-gated ionchannel; ω-conotoxins and the voltage-gated calcium channel;κ-conotoxins and the voltage-gated sodium channel; δ-conotoxins and thevoltage-gated sodium channel; κ-conotoxins and the voltage-gatedpotassium channel; conantokins and the ligand-gated glutamate (NMDA)channel.

[0007] However, the structure and function of only a small minority ofthese peptides have been determined to date. For peptides where functionhas been determined, three classes of targets have been elucidated:voltage-gated ion channels; ligand-gated ion channels, andG-protein-linked receptors.

[0008] Conus peptides which target voltage-gated ion channels includethose that delay the inactivation of sodium channels, as well asblockers specific for sodium channels, calcium channels and potassiumchannels. Peptides that target ligand-gated ion channels includeantagonists of NMDA and serotonin receptors, as well as competitive andnoncompetitive nicotinic receptor antagonists. Peptides which act onG-protein receptors include neurotensin and vasopressin receptoragonists. The unprecedented pharmaceutical selectivity of conotoxins isat least in part defined by a specific disulfide bond frameworkscombined with hypervariable amino acids within disulfide loops (for areview see McIntosh et al., 1998).

[0009] There are drugs used in the treatment of pain, which are known inthe literature and to the skilled artisan. See, for example, MerckManual, 16th Ed. (1992). However, there is a demand for more activeanalgesic agents with diminished side effects and toxicity and which arenon-addictive. The ideal analgesic would reduce the awareness of pain,produce analgesia over a wide range of pain types, act satisfactorilywhether given orally or parenterally, produce minimal or no sideeffects, be free from tendency to produce tolerance and drug dependence.

[0010] Due to the high potency and exquisite selectivity of theconopeptides, several are in various stages of clinical development fortreatment of human disorders. For example, two Conus peptides are beingdeveloped for the treatment of pain. The most advanced is ω-conotoxinMVIIA (ziconotide), an N-type calcium channel blocker (see Heading, C.,1999; U.S. Pat. No. 5,859,186). (ω-Conotoxin MVIIA, isolated from Conusmagus, is approximately 1000 times more potent than morphine, yet doesnot produce the tolerance or addictive properties of opiates.ω-Conotoxin MVIIA has completed Phase III (final stages) of humanclinical trials and has been approved as a therapeutic agent.ω-Conotoxin MVIIA is introduced into human patients by means of animplantable, programmable pump with a catheter threaded into theintrathecal space. Preclinical testing for use in post-surgical pain isbeing carried out on another Conus peptide, contulakin-G, isolated fromConus geographus (Craig et al. 1999). Contulakin-G is a 16 amino acidO-linked glycopeptide whose C-terminus resembles neurotensin. It is anagonist of neurotensin receptors, but appears significantly more potentthan neurotensin in inhibiting pain in in vivo assays.

[0011] In view of a large number of biologically active substances inConus species it is desirable to further characterize them and toidentify peptides capable of treating disorders voltage-gated ionchannels, ligand-gated ion channels and/or receptors. Surprisingly, andin accordance with this invention, Applicants have discovered novelconotoxins that can be useful for the treatment of disorders involvingvoltage-gated ion channels, ligand-gated ion channels and/or receptorsand could address a long felt need for a safe and effective treatment.

SUMMARY OF THE INVENTION

[0012] The present invention is directed to conotoxin peptides,derivatives or pharmaceutically acceptable salts thereof. The presentinvention is further directed to the use of this peptide, derivativesthereof and pharmaceutically acceptable salts thereof for the treatmentof disorders associated with voltage-gated ion channels, ligand-gatedion channels and/or receptors. The invention is further directed tonucleic acid sequences encoding the conotoxin peptides and encodingpropeptides, as well as the propeptides.

[0013] More specifically, the present invention is directed to conotoxinpeptides, having the amino acid sequences set forth in Tables 1-14below.

[0014] The present invention is also directed to derivatives orpharmaceutically acceptable salts of the conotoxin peptides or thederivatives. Examples of derivatives include peptides in which the Argresidues may be substituted by Lys, omithine, homoargine, nor-Lys,N-methyl-Lys, N,N-dimethyl-Lys, N,N,N-trimethyl-Lys or any syntheticbasic amino acid; the Lys residues may be substituted by Arg, omithine,homoargine, nor-Lys, or any synthetic basic amino acid; the Tyr residuesmay be substituted with meta-Tyr, ortho-Tyr, nor-Tyr, mono-halo-Tyr,di-halo-Tyr, O-sulpho-Tyr, O-phospho-Tyr, nitro-Tyr or any synthetichydroxy containing amino acid; the Ser residues may be substituted withThr or any synthetic hydroxylated amino acid; the Thr residues may besubstituted with Ser or any synthetic hydroxylated amino acid; the Pheresidues may be substituted with any synthetic aromatic amino acid; theTrp residues may be substituted with Trp (D), neo-Trp, halo-Trp (D or L)or any aromatic synthetic amino acid; and the Asn, Ser, Thr or Hypresidues may be glycosylated. The halogen may be iodo, chloro, fluoro orbromo; preferably iodo for halogen substituted-Tyr and bromo forhalogen-substituted Trp. The Tyr residues may also be substituted withthe 3-hydroxyl or 2-hydroxyl isomers (meta-Tyr or ortho-Tyr,respectively) and corresponding O-sulpho- and O-phospho-derivatives. Theacidic amino acid residues may be substituted with any synthetic acidicamino acid, e.g., tetrazolyl derivatives of Gly and Ala. The aliphaticamino acids may be substituted by synthetic derivatives bearingnon-natural aliphatic branched or linear side chains C_(n)H_(2n+2) up toand including n=8. The Leu residues may be substituted with Leu (D). TheGlu residues may be substituted with Gla. The Gla residues may besubstituted with Glu. The N-terminal Gln residues may be substitutedwith pyroGlu. The Met residues may be substituted with norleucine (Nle).The Cys residues may be in D or L configuration and may optionally besubstituted with homocysteine (D or L).

[0015] Examples of synthetic aromatic amino acid include, but are notlimited to, nitro-Phe, 4-substituted-Phe wherein the substituent isC₁-C₃ alkyl, carboxyl, hyrdroxymethyl, sulphomethyl, halo, phenyl, —CHO,—CN, —SO₃H and —NHAc. Examples of synthetic hydroxy containing aminoacid, include, but are not limited to, such as 4-hydroxymethyl-Phe,4-hydroxyphenyl-Gly, 2,6-dimethyl-Tyr and 5-amino-Tyr. Examples ofsynthetic basic amino acids include, but are not limited to,N-1-(2-pyrazolinyl)-Arg, 2-(4-piperinyl)-Gly, 2-(4-piperinyl)-Ala,2-[3-(2S)pyrrolininyl)]-Gly and 2-[3-(2S)pyrrolininyl)]-Ala. These andother synthetic basic amino acids, synthetic hydroxy containing aminoacids or synthetic aromatic amino acids are described in Building BlockIndex, Version 3.0 (1999 Catalog, pages 4-47 for hydroxy containingamino acids and aromatic amino acids and pages 66-87 for basic aminoacids; see also http://www.amino-acids.com), incorporated herein byreference, by and available from RSP Amino Acid Analogues, Inc.,Worcester, Mass. The residues containing protecting groups aredeprotected using conventional techniques. Examples of synthetic acidamino acids include those derivatives bearing acidic functionality,including carboxyl, phosphate, sulfonate and synthetic tetrazolylderivatives such as described by Ornstein et al. (1993) and in U.S. Pat.No. 5,331,001, each incorporated herein by reference, and such as shownin the following schemes 1-3.

[0016] Optionally, in the conotoxin peptides of the present invention,the Asn residues may be modified to contain an N-glycan and the Ser, Thrand Hyp residues may be modified to contain an O-glycan (e.g., g-N, g-S,g-T and g-Hyp). In accordance with the present invention, a glycan shallmean any N-, S- or O-linked mono-, di-, tri-, poly- or oligosaccharidethat can be attached to any hydroxy, amino or thiol group of natural ormodified amino acids by synthetic or enzymatic methodologies known inthe art. The monosaccharides making up the glycan can include D-allose,D-altrose, D-glucose, D-mannose, D-gulose, D-idose, D-galactose,D-talose, D-galactosamine, D-glucosamine, D-N-acetyl-glucosamine(GlcNAc), D-N-acetyl-galactosamine (GalNAc), D-fucose or D-arabinose.These saccharides may be structurally modified, e.g., with one or moreO-sulfate, O-phosphate, O-acetyl or acidic groups, such as sialic acid,including combinations thereof. The gylcan may also include similarpolyhydroxy groups, such as D-penicillamine 2,5 and halogenatedderivatives thereof or polypropylene glycol derivatives. The glycosidiclinkage is beta and 1-4 or 1-3, preferably 1-3. The linkage between theglycan and the amino acid may be alpha or beta, preferably alpha and is1-.

[0017] Core O-glycans have been described by Van de Steen et al. (1998),incorporated herein by reference. Mucin type O-linked oligosaccharidesare attached to Ser or Thr (or other hydroxylated residues of thepresent peptides) by a GalNAc residue. The monosaccharide buildingblocks and the linkage attached to this first GalNAc residue define the“core glycans,” of which eight have been identified. The type ofglycosidic linkage (orientation and connectivities) are defined for eachcore glycan. Suitable glycans and glycan analogs are described furtherin U.S. Ser. No. 09/420,797 filed Oct. 19, 1999 and in PCT ApplicationNo. PCT/US99/24380 filed Oct. 19, 1999 (PCT Published Application No. WO00/23092), each incorporated herein by reference. A preferred glycan isGal(β1 →3GalNAc(β1→).

[0018] Optionally, in the conotoxin peptides described above, pairs ofCys residues may be replaced pairwise with isoteric lactam orester-thioether replacements, such as Ser/(Glu or Asp), Lys/(Glu orAsp), Cys/(Glu or Asp) or Cys/Ala combinations. Sequential coupling byknown methods (Barnay et al., 2000; Hruby et al., 1994; Bitan et al.,1997) allows replacement of native Cys bridges with lactam bridges.Thioether analogs may be readily synthesized using halo-Ala residuescommercially available from RSP Amino Acid Analogues. In addition,individual Cys residues may be replaced with homoCys, seleno-Cys orpenicillamine, so that disulfide bridges may be formed betweenCys-homoCys or Cys-penicillamine, or homoCys-penicllamine and the like.

[0019] The present invention is further directed to derivatives of theabove peptides and peptide derivatives which are acylic permutations inwhich the cyclic permutants retain the native bridging pattern of nativetoxin. See, Craik et al. (2001).

[0020] The present invention is further directed to a method of treatingdisorders associated with voltage-gated ion channels, ligand-gated ionchannels and/or receptor disorders in a subject comprising administeringto the subject an effective amount of the pharmaceutical compositioncomprising a therapeutically effective amount of a conotoxin peptidedescribed herein or a pharmaceutically acceptable salt or solvatethereof. The present invention is also directed to a pharmaceuticalcomposition comprising a therapeutically effective amount of a conotoxinpeptide described herein or a pharmaceutically acceptable salt orsolvate thereof and a pharmaceutically acceptable carrier.

[0021] More specifically, the present invention is also directed tonucleic acids which encode conotoxin peptides of the present inventionor which encodes precursor peptides for these conotoxin peptides, aswell as the precursor peptide. The nucleic acid sequences encoding theprecursor peptides of other conotoxin peptides of the present inventionare set forth in Table 1. Table 1 also sets forth the amino acidsequences of these precursor peptides.

[0022] Another embodiment of the invention contemplates a method ofidentifying compounds that mimic the therapeutic activity of the instantpeptide, comprising the steps of: (a) conducting a biological assay on atest compound to determine the therapeutic activity; and (b) comparingthe results obtained from the biological assay of the test compound tothe results obtained from the biological assay of the peptide. Thepeptide is labeled with any conventional label, preferably a radioiodineon an available Tyr. Thus, the invention is also directed toradioiodinated conotoxins.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

[0023] The present invention is directed to conotoxin peptides,derivatives or pharmaceutically acceptable salts thereof. The presentinvention is further directed to the use of this peptide, derivativesthereof and pharmaceutically acceptable salts thereof for the treatmentof disorders associated with voltage-gated ion channels, ligand-gatedion channels and/or receptors. The invention is further directed tonucleic acid sequences encoding the conotoxin peptides and encodingpropeptides, as well as the propeptides.

[0024] The present invention, in another aspect, relates to apharmaceutical composition comprising an effective amount of a conotoxinpeptides, a mutein thereof, an analog thereof, an active fragmentthereof or pharmaceutically acceptable salts or solvates. Such apharmaceutical composition has the capability of acting at voltage-gatedion channels, ligand-gated ion channels and/or receptors, and are thususeful for treating a disorder or disease of a living animal body,including a human, which disorder or disease is responsive to thepartial or complete blockade of such channels or receptors comprisingthe step of administering to such a living animal body, including ahuman, in need thereof a therapeutically effective amount of apharmaceutical composition of the present invention.

[0025] Examples of voltage-gated ion channels include the voltage-gatedcalcium channel, the voltage-gated sodium channel, the voltage-gatedpotassium channel and the proton-gated ion channel. Examples ofligand-gated channels include the nicotinic ligand-gated ion channel,ligand-gated glutamate (NMDA) channel and the ligand-gated 5HT₃(serotonin) channel. Examples of receptors include the G-proteinreceptors. Activity of ψ-conotoxins is described in U.S. Pat. No.5,969,096 and in Shon et al. (1997). Activity of bromosleeper conotoxinsis described in U.S. Pat. No. 5,889,147 and in Craig et al. (1997).Activity of σ-conotoxins is described in U.S. Pat. No. 5,889,147.Activity of contryphan conotoxins is described in U.S. Pat. No.6,077,934 and in Jimenez et al. (1996). Activity of conopressins isdescribed in Cruz et al. (1987) and in Kruszynski et al. (1990).Activity of γ-conotoxins is described in Fainzilber et al. (1998).Activity of αA-conotoxins (kappaA??) is described in Jacobsen et al.(1997) and in Hopkins et al. (1995). Activity of α-conotoxins isdescribed in U.S. Pat. Nos. 4,447,356 and 5,514,774. Activity ofτ-conotoxins is described in U.S. Ser. No. 09/497,491 (PCT/US00/03021,PCT published application WO 00/46371) as an antagonist foracetylcholine receptors and as analgesic agents for the treatment ofpain (whether acute or chronic), including migraine, chronic pain, andneuropathic pain, without undesirable side effects. Activity ofcontulakins is described in U.S. Ser. No. 09/420,797 (PCT/US99/24380,PCT published application WO 00/23092). Each of these references isincorporated herein by reference.

[0026] Since σ-conotoxins are antagonists of the 5HT₃ receptor, they arealso useful in treating irritable bowel syndrome (IBS) and visceralpain. Visceral pain is a common experience in health and disease.Chronic visceral hyperalgesia in the absence of detectable organicdisease has been implicated in many common functional bowel disorders(FDB), such as IBS, non-ulcer dyspepsia (NUD) and non-cardiac chest pain(NCCP).

[0027] Pain in IBS cannot be explained by normal perception of abnormalmotility. In the majority of patients, sensory perception itself isabnormal. Most visceral afferent information is part of the reflexactivity of digestion and does not reach concious perception. Increasingevidence suggests that long term changes in the the thresholds and gainof the visceral afferent pathways are present in patients with FDBs.This has been referred to as visceral hyperalgesia (Mayer et al., 1994).

[0028] It has been proposed that FDBs are a result of increasedexcitability of spinal neurones. According to their model, many inputscan result in transient, short term, or life long sensitization ofafferent pathways involved in visceral reflexes and sensations from thegut. The increased sensory input to interneurons and/or dorsal hornneurons in the spinal cord will result in secondary hyperalgesia, inwhich adjacent, undamaged viscera develop sensitivity to normalinnocuous stimuli (allodynia), and central hyperexcitability as aconsequence of changes in the circuitary of the dorsal horn. Thiscentral sensitization may subsequently extend to supraspinal centersalso.

[0029] Altered spinal processing of visceral sensory information canexplain altered sensory thresholds and altered referral patterns, theperception of visceral sensations without stimulation of visceralmechnoreceptors (sensation of incomplete evacuation), and thesymptomatic involvement of multiple sites in the GI tract, includingextra intestinal sites. Increased excitability of dorsal horn neurones,resulting in the recruitment of previously sub-threshold inputs, mayexplain cutaneous allodynia in some patients with IBS, burningsensations referred to different parts of the body, coldhypersensitivity and pain referral to upper and lower extremities.

[0030] A number of compounds have been shown to modulate visceralsensitivity in IBS patients. These include octreotide (sst₂; Novartis),the 5-HT₃ antgonists odansetron (Glaxo) and granisetron (SKB) and theperipheral kappa opioid agonist, fedotozine (Jouveinal SA). The 5-HT₃antagonist alosteron (Glaxo), cuurrently in development for IBS, isactive in modifying the perception of colonic distension and gutcompliance in IBS patients. New drugs in development for the treatmentof IBS that are targeted at pain control as well as dysmotility include5-HT₃ and 5-HT₄ receptor antagonists. 5-HT₃ receptors are locatedthroughout the central and peripheral nervous system—their role inmodulating the activity of visceral afferent and enteric neurones hasled to the proposal that 5-HT acts as a sensitizing agent via thesereceptors on visceral afferent neurones. 5-HT₃ receptor antagonists havebeen widely reported to attenuate blood pressure responses to intestinaldistension. 5-HT₃ antagonists in development for IBS include Alosteron(phase III), which is reported to reduce abdominal pain, slow colonictransit and increase colon compliance in IBS patients. Other compoundswith positive effects include the antiemetic Ramosteron (Yamanouchi),Cilansteron (Solvay) and YM-114 (Yamanouchi). An animal model fordysmotility of the GI tract has been described by Maric et al. (1989).

[0031] The conotoxin peptides described herein are sufficiently small tobe chemically synthesized. General chemical syntheses for preparing theforegoing conotoxin peptides are described hereinafter. Various ones ofthe conotoxin peptides can also be obtained by isolation andpurification from specific Conus species using the technique describedin U.S. Pat. Nos. 4,447,356 (Olivera et al., 1984); 5,514,774;5,719,264; and 5,591,821, as well as in PCT published application WO98/03189, the disclosures of which are incorporated herein by reference.

[0032] Although the conotoxin peptides of the present invention can beobtained by purification from cone snails, because the amounts ofconotoxin peptides obtainable from individual snails are very small, thedesired substantially pure conotoxin peptides are best practicallyobtained in commercially valuable amounts by chemical synthesis usingsolid-phase strategy. For example, the yield from a single cone snailmay be about 10 micrograms or less of conotoxin peptides peptide. By“substantially pure” is meant that the peptide is present in thesubstantial absence of other biological molecules of the same type; itis preferably present in an amount of at least about 85% purity andpreferably at least about 95% purity. Chemical synthesis of biologicallyactive conotoxin peptides peptides depends of course upon correctdetermination of the amino acid sequence.

[0033] The conotoxin peptides can also be produced by recombinant DNAtechniques well known in the art. Such techniques are described bySambrook et al. (1989). A gene of interest (i.e., a gene that encodes asuitable conotoxin peptides) can be inserted into a cloning site of asuitable expression vector by using standard techniques. Thesetechniques are well known to those skilled in the art. The expressionvector containing the gene of interest may then be used to transfect thedesired cell line. Standard transfection techniques such as calciumphosphate co-precipitation, DEAE-dextran transfection or electroporationmay be utilized. A wide variety of host/expression vector combinationsmay be used to express a gene encoding a conotoxin peptide of interest.Such combinations are well known to a skilled artisan. The peptidesproduced in this manner are isolated, reduced if necessary, and oxidizedto form the correct disulfide bonds.

[0034] One method of forming disulfide bonds in the conotoxin peptidesof the present invention is the air oxidation of the linear peptides forprolonged periods under cold room temperatures or at room temperature.This procedure results in the creation of a substantial amount of thebioactive, disulfide-linked peptides. The oxidized peptides arefractionated using reverse-phase high performance liquid chromatography(HPLC) or the like, to separate peptides having different linkedconfigurations. Thereafter, either by comparing these fractions with theelution of the native material or by using a simple assay, theparticular fraction having the correct linkage for maximum biologicalpotency is easily determined. However, because of the dilution resultingfrom the presence of other fractions of less biopotency, a somewhathigher dosage may be required.

[0035] The peptides are synthesized by a suitable method, such as byexclusively solid-phase techniques, by partial solid-phase techniques,by fragment condensation or by classical solution couplings.

[0036] In conventional solution phase peptide synthesis, the peptidechain can be prepared by a series of coupling reactions in whichconstituent amino acids are added to the growing peptide chain in thedesired sequence. Use of various coupling reagents, e.g.,dicyclohexylcarbodiimide or diisopropylcarbonyldimidazole, variousactive esters, e.g., esters of N-hydroxyphthalimide orN-hydroxy-succinimide, and the various cleavage reagents, to carry outreaction in solution, with subsequent isolation and purification ofintermediates, is well known classical peptide methodology. Classicalsolution synthesis is described in detail in the treatise, “Methoden derOrganischen Chemie (Houben-Weyl): Synthese von Peptiden,” (1974).Techniques of exclusively solid-phase synthesis are set forth in thetextbook, “Solid-Phase Peptide Synthesis,” (Stewart and Young, 1969),and are exemplified by the disclosure of U.S. Pat. No. 4,105,603 (Valeet al., 1978). The fragment condensation method of synthesis isexemplified in U.S. Pat. No. 3,972,859 (1976). Other available synthesesare exemplified by U.S. Pat. Nos. 3,842,067 (1974) and 3,862,925 (1975).The synthesis of peptides containing γ-carboxyglutamic acid residues isexemplified by Rivier et al. (1987), Nishiuchi et al. (1993) and Zhou etal. (1996).

[0037] Common to such chemical syntheses is the protection of the labileside chain groups of the various amino acid moieties with suitableprotecting groups which will prevent a chemical reaction from occurringat that site until the group is ultimately removed. Usually also commonis the protection of an α-amino group on an amino acid or a fragmentwhile that entity reacts at the carboxyl group, followed by theselective removal of the α-amino protecting group to allow subsequentreaction to take place at that location. Accordingly, it is common that,as a step in such a synthesis, an intermediate compound is producedwhich includes each of the amino acid residues located in its desiredsequence in the peptide chain with appropriate side-chain protectinggroups linked to various ones of the residues having labile side chains.

[0038] As far as the selection of a side chain amino protecting group isconcerned, generally one is chosen which is not removed duringdeprotection of the α-amino groups during the synthesis. However, forsome amino acids, e.g., His, protection is not generally necessary. Inselecting a particular side chain protecting group to be used in thesynthesis of the peptides, the following general rules are followed: (a)the protecting group preferably retains its protecting properties and isnot split off under coupling conditions, (b) the protecting group shouldbe stable under the reaction conditions selected for removing theα-amino protecting group at each step of the synthesis, and (c) the sidechain protecting group must be removable, upon the completion of thesynthesis containing the desired amino acid sequence, under reactionconditions that will not undesirably alter the peptide chain.

[0039] It should be possible to prepare many, or even all, of thesepeptides using recombinant DNA technology. However, when peptides arenot so prepared, they are preferably prepared using the Merrifieldsolid-phase synthesis, although other equivalent chemical synthesesknown in the art can also be used as previously mentioned. Solid-phasesynthesis is commenced from the C-terminus of the peptide by coupling aprotected α-amino acid to a suitable resin. Such a starting material canbe prepared by attaching an α-amino-protected amino acid by an esterlinkage to a chloromethylated resin or a hydroxymethyl resin, or by anamide bond to a benzhydrylamine (BHA) resin or paramethylbenzhydrylamine(MBHA) resin. Preparation of the hydroxymethyl resin is described byBodansky et al. (1966). Chloromethylated resins are commerciallyavailable from Bio Rad Laboratories (Richmond, Calif.) and from Lab.Systems, Inc. The preparation of such a resin is described by Stewartand Young (1969). BHA and MBHA resin supports are commerciallyavailable, and are generally used when the desired polypeptide beingsynthesized has an unsubstituted amide at the C-terminus. Thus, solidresin supports may be any of those known in the art, such as one havingthe formulae —O—CH₂-resin support, —NH BHA resin support, or —NH—MBHAresin support. When the unsubstituted amide is desired, use of a BHA orMBHA resin is preferred, because cleavage directly gives the amide. Incase the N-methyl amide is desired, it can be generated from an N-methylBHA resin. Should other substituted amides be desired, the teaching ofU.S. Pat. No. 4,569,967 (Kornreich et al., 1986) can be used, or shouldstill other groups than the free acid be desired at the C-terminus, itmay be preferable to synthesize the peptide using classical methods asset forth in the Houben-Weyl text (1974).

[0040] The C-terminal amino acid, protected by Boc or Fmoc and by aside-chain protecting group, if appropriate, can be first coupled to achloromethylated resin according to the procedure set forth in K. Horikiet al. (1978), using KF in DMF at about 60° C. for 24 hours withstirring, when a peptide having free acid at the C-terminus is to besynthesized. Following the coupling of the BOC-protected amino acid tothe resin support, the α-amino protecting group is removed, as by usingtrifluoroacetic acid (TFA) in methylene chloride or TFA alone. Thedeprotection is carried out at a temperature between about 0° C. androom temperature. Other standard cleaving reagents, such as HCl indioxane, and conditions for removal of specific α-amino protectinggroups may be used as described in Schroder & Lubke (1965).

[0041] After removal of the α-amino-protecting group, the remainingα-amino- and side chain-protected amino acids are coupled step-wise inthe desired order to obtain the intermediate compound definedhereinbefore, or as an alternative to adding each amino acid separatelyin the synthesis, some of them may be coupled to one another prior toaddition to the solid phase reactor. Selection of an appropriatecoupling reagent is within the skill of the art. Particularly suitableas a coupling reagent is N,N′-dicyclohexylcarbodiimide (DCC, DIC, HBTU,HATU, TBTU in the presence of HoBt or HoAt).

[0042] The activating reagents used in the solid phase synthesis of thepeptides are well known in the peptide art. Examples of suitableactivating reagents are carbodiimides, such asN,N′-diisopropylcarbodiimide andN-ethyl-N′-(3-dimethylaminopropyl)carbodiimide. Other activatingreagents and their use in peptide coupling are described by Schroder &Lubke (1965) and Kapoor (1970).

[0043] Each protected amino acid or amino acid sequence is introducedinto the solid-phase reactor in about a twofold or more excess, and thecoupling may be carried out in a it medium of dimethylformamide(DMF):CH₂Cl₂ (1:1) or in DMF or CH₂Cl₂ alone. In cases whereintermediate coupling occurs, the coupling procedure is repeated beforeremoval of the α-amino protecting group prior to the coupling of thenext amino acid. The success of the coupling reaction at each stage ofthe synthesis, if performed manually, is preferably monitored by theninhydrin reaction, as described by Kaiser et al. (1970). Couplingreactions can be performed automatically, as on a Beckman 990 automaticsynthesizer, using a program such as that reported in Rivier et al.(1978).

[0044] After the desired amino acid sequence has been completed, theintermediate peptide can be removed from the resin support by treatmentwith a reagent, such as liquid hydrogen fluoride or TFA (if using Fmocchemistry), which not only cleaves the peptide from the resin but alsocleaves all remaining side chain protecting groups and also the -aminoprotecting group at the N-terminus if it was not previously removed toobtain the peptide in the form of the free acid. If Met is present inthe sequence, the Boc protecting group is preferably first removed usingtrifluoroacetic acid (TFA)/ethanedithiol prior to cleaving the peptidefrom the resin with HF to eliminate potential S-alkylation. When usinghydrogen fluoride or TFA for cleaving, one or more scavengers such asanisole, cresol, dimethyl sulfide and methylethyl sulfide are includedin the reaction vessel.

[0045] Cyclization of the linear peptide is preferably affected, asopposed to cyclizing the peptide while a part of the peptido-resin, tocreate bonds between Cys residues. To effect such a disulfide cyclizinglinkage, fully protected peptide can be cleaved from a hydroxymethylatedresin or a chloromethylated resin support by ammonolysis, as is wellknown the art, to yield the fully protected amide intermediate, which isthereafter suitably cyclized and deprotected. Alternatively,deprotection, as well as cleavage of the peptide from the above resinsor a benzhydrylamine (BHA) resin or a methylbenzhydrylamine (MBHA), cantake place at 0° C. with hydrofluoric acid (HF) or TFA, followed byoxidation as described above.

[0046] The peptides are also synthesized using an automatic synthesizer.Amino acids are sequentially coupled to an MBHA Rink resin (typically100 mg of resin) beginning at the C-terminus using an Advanced Chemtech357 Automatic Peptide Synthesizer. Couplings are carried out using1,3-diisopropylcarbodimide in N-methylpyrrolidinone (NMP) or by2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate(HBTU) and diethylisopropylethylamine (DIEA). The FMOC protecting groupis removed by treatment with a 20% solution of piperidine indimethylformamide(DMF). Resins are subsequently washed with DMF (twice),followed by methanol and NMP.

[0047] Muteins, analogs or active fragments, of the foregoing conotoxinpeptides are also contemplated here. See, e.g., Hammerland et al.(1992). Derivative muteins, analogs or active fragments of the conotoxinpeptides may be synthesized according to known techniques, includingconservative amino acid substitutions, such as outlined in U.S. Pat.Nos. 5,545,723 (see particularly col. 2, line 50—col. 3, line 8);5,534,615 (see particularly col. 19, line 45—col. 22, line 33); and5,364,769 (see particularly col. 4, line 55—col. 7, line 26), eachherein incorporated by reference.

[0048] Pharmaceutical compositions containing a compound of the presentinvention as the active ingredient can be prepared according toconventional pharmaceutical compounding techniques. See, for example,Remington's Pharmaceutical Sciences, 18th Ed. (1990, Mack PublishingCo., Easton, Pa.). Typically, an antagonistic amount of activeingredient will be admixed with a pharmaceutically acceptable carrier.The carrier may take a wide variety of forms depending on the form ofpreparation desired for administration, e.g., intravenous, oral,parenteral or intrathecally. For examples of delivery methods see U.S.Pat. No. 5,844,077, incorporated herein by reference.

[0049] “Pharmaceutical composition” means physically discrete coherentportions suitable for medical administration. “Pharmaceuticalcomposition in dosage unit form” means physically discrete coherentunits suitable for medical administration, each containing a daily doseor a multiple (up to four times) or a sub-multiple (down to a fortieth)of a daily dose of the active compound in association with a carrierand/or enclosed within an envelope. Whether the composition contains adaily dose, or for example, a half, a third or a quarter of a dailydose, will depend on whether the pharmaceutical composition is to beadministered once or, for example, twice, three times or four times aday, respectively.

[0050] The term “salt”, as used herein, denotes acidic and/or basicsalts, formed with inorganic or organic acids and/or bases, preferablybasic salts. While pharmaceutically acceptable salts are preferred,particularly when employing the compounds of the invention asmedicaments, other salts find utility, for example, in processing thesecompounds, or where non-medicament-type uses are contemplated. Salts ofthese compounds may be prepared by art-recognized techniques.

[0051] Examples of such pharmaceutically acceptable salts include, butare not limited to, inorganic and organic addition salts, such ashydrochloride, sulphates, nitrates or phosphates and acetates,trifluoroacetates, propionates, succinates, benzoates, citrates,tartrates, fumarates, maleates, methane-sulfonates, isothionates,theophylline acetates, salicylates, respectively, or the like. Loweralkyl quaternary ammonium salts and the like are suitable, as well.

[0052] As used herein, the term “pharmaceutically acceptable” carriermeans a non-toxic, inert solid, semi-solid liquid filler, diluent,encapsulating material, formulation auxiliary of any type, or simply asterile aqueous medium, such as saline. Some examples of the materialsthat can serve as pharmaceutically acceptable carriers are sugars, suchas lactose, glucose and sucrose, starches such as corn starch and potatostarch, cellulose and its derivatives such as sodium carboxymethylcellulose, ethyl cellulose and cellulose acetate; powdered tragacanth;malt, gelatin, talc; excipients such as cocoa butter and suppositorywaxes; oils such as peanut oil, cottonseed oil, safflower oil, sesameoil, olive oil, corn oil and soybean oil; glycols, such as propyleneglycol, polyols such as glycerin, sorbitol, mannitol and polyethyleneglycol; esters such as ethyl oleate and ethyl laurate, agar; bufferingagents such as magnesium hydroxide and aluminum hydroxide; alginic acid;pyrogen-free water; isotonic saline, Ringer's solution; ethyl alcoholand phosphate buffer solutions, as well as other non-toxic compatiblesubstances used in pharmaceutical formulations.

[0053] Wetting agents, emulsifiers and lubricants such as sodium laurylsulfate and magnesium stearate, as well as coloring agents, releasingagents, coating agents, sweetening, flavoring and perfuming agents,preservatives and antioxidants can also be present in the composition,according to the judgment of the formulator. Examples ofpharmaceutically acceptable antioxidants include, but are not limitedto, water soluble antioxidants such as ascorbic acid, cysteinehydrochloride, sodium bisulfite, sodium metabisulfite, sodium sulfite,and the like; oil soluble antioxidants, such as ascorbyl palmitate,butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT),lecithin, propyl gallate, aloha-tocopherol and the like; and the metalchelating agents such as citric acid, ethylenediamine tetraacetic acid(EDTA), sorbitol, tartaric acid, phosphoric acid and the like.

[0054] For oral administration, the compounds can be formulated intosolid or liquid preparations such as capsules, pills, tablets, lozenges,melts, powders, suspensions or emulsions. In preparing the compositionsin oral dosage form, any of the usual pharmaceutical media may beemployed, such as, for example, water, glycols, oils, alcohols,flavoring agents, preservatives, coloring agents, suspending agents, andthe like in the case of oral liquid preparations (such as, for example,suspensions, elixirs and solutions); or carriers such as starches,sugars, diluents, granulating agents, lubricants, binders,disintegrating agents and the like in the case of oral solidpreparations (such as, for example, powders, capsules and tablets).Because of their ease in administration, tablets and capsules representthe most advantageous oral dosage unit form, in which case solidpharmaceutical carriers are obviously employed. If desired, tablets maybe sugar-coated or enteric-coated by standard techniques. The activeagent can be encapsulated to make it stable to passage through thegastrointestinal tract while at the same time allowing for passageacross the blood brain barrier. See for example, WO 96/11698.

[0055] For parenteral administration, the compound may be dissolved in apharmaceutical carrier and administered as either a solution or asuspension. Illustrative of suitable carriers are water, saline,dextrose solutions, fructose solutions, ethanol, or oils of animal,vegetative or synthetic origin. The carrier may also contain otheringredients, for example, preservatives, suspending agents, solubilizingagents, buffers and the like. When the compounds are being administeredintrathecally, they may also be dissolved in cerebrospinal fluid.

[0056] A variety of administration routes are available. The particularmode selected will depend of course, upon the particular drug selected,the severity of the disease state being treated and the dosage requiredfor therapeutic efficacy. The methods of this invention, generallyspeaking, may be practiced using any mode of administration that ismedically acceptable, meaning any mode that produces effective levels ofthe active compounds without causing clinically unacceptable adverseeffects. Such modes of administration include oral, rectal, sublingual,topical, nasal, transdermal or parenteral routes. The term “parenteral”includes subcutaneous, intravenous, epidural, irrigation, intramuscular,release pumps, or infusion.

[0057] For example, administration of the active agent according to thisinvention may be achieved using any suitable delivery means, including:

[0058] (a) pump (see, e.g., Luer & Hatton (1993), Zimm et al. (1984) andEttinger et al. (1978));

[0059] (b), microencapsulation (see, e.g., U.S. Pat. Nos. 4,352,883;4,353,888; and 5,084,350);

[0060] (c) continuous release polymer implants (see, e.g., U.S. Pat. No.4,883,666);

[0061] (d) macroencapsulation (see, e.g., U.S. Pat. Nos. 5,284,761,5,158,881, 4,976,859 and 4,968,733 and published PCT patent applicationsWO92/19195, WO 95/05452);

[0062] (e) naked or unencapsulated cell grafts to the CNS (see, e.g.,U.S. Pat. Nos. 5,082,670 and 5,618,531);

[0063] (f) injection, either subcutaneously, intravenously,intra-arterially, intramuscularly, or to other suitable site; or

[0064] (g) oral administration, in capsule, liquid, tablet, pill, orprolonged release formulation.

[0065] In one embodiment of this invention, an active agent is delivereddirectly into the CNS, preferably to the brain ventricles, brainparenchyma, the intrathecal space or other suitable CNS location, mostpreferably intrathecally.

[0066] Alternatively, targeting therapies may be used to deliver theactive agent more specifically to certain types of cell, by the use oftargeting systems such as antibodies or cell specific ligands. Targetingmay be desirable for a variety of reasons, e.g. if the agent isunacceptably toxic, or if it would otherwise require too high a dosage,or if it would not otherwise be able to enter the target cells.

[0067] The active agents, which are peptides, can also be administeredin a cell based delivery system in which a DNA sequence encoding anactive agent is introduced into cells designed for implantation in thebody of the patient, especially in the spinal cord region. Suitabledelivery systems are described in U.S. Pat. No. 5,550,050 and publishedPCT Application Nos. WO 92/19195, WO 94/25503, WO 95/01203, WO 95/05452,WO 96/02286, WO 96/02646, WO 96/40871, WO 96/40959 and WO 97/12635.Suitable DNA sequences can be prepared synthetically for each activeagent on the basis of the developed sequences and the known geneticcode.

[0068] Exemplary methods for administering such muscle relaxantcompounds (e.g., so as to achieve sterile or aseptic conditions) will beapparent to the skilled artisan. Certain methods suitable foradministering compounds useful according to the present invention areset forth in Goodman and Gilman's The Pharmacological Basis ofTherapeutics, 7th Ed. (1985). The administration to the patient can beintermittent; or at a gradual, continuous, constant or controlled rate.Administration can be to a warm-blooded animal (e.g. a mammal, such as amouse, rat, cat, rabbit, dog, pig, cow or monkey); but advantageously isadministered to a human being. Administration occurs after generalanesthesia is administered. The frequency of administration normally isdetermined by an anesthesiologist, and typically varies from patient topatient.

[0069] The active agent is preferably administered in an therapeuticallyeffective amount. By a “therapeutically effective amount” or simply“effective amount” of an active compound is meant a sufficient amount ofthe compound to treat the desired condition at a reasonable benefit/riskratio applicable to any medical treatment. The actual amountadministered, and the rate and time-course of administration, willdepend on the nature and severity of the condition being treated.Prescription of treatment, e.g. decisions on dosage, timing, etc., iswithin the responsibility of general practitioners or spealists, andtypically takes account of the disorder to be treated, the condition ofthe individual patient, the site of delivery, the method ofadministration and other factors known to practitioners. Examples oftechniques and protocols can be found in Remington 's ParmaceuticalSciences.

[0070] Dosage may be adjusted appropriately to achieve desired druglevels, locally or systemically. Typically the active agents of thepresent invention exhibit their effect at a dosage range from about0.001 mg/kg to about 250 mg/kg, preferably from about 0.01 mg/kg toabout 100 mg/kg of the active ingredient, more preferably from a bout0.05 mg/kg to about 75 mg/kg. A suitable dose can be administered inmultiple sub-doses per day. Typically, a dose or sub-dose may containfrom about 0.1 mg to about 500 mg of the active ingredient per unitdosage form. A more preferred dosage will contain from about 0.5 mg toabout 100 mg of active ingredient per unit dosage form. Dosages aregenerally initiated at lower levels and increased until desired effectsare achieved. In the event that the response in a subject isinsufficient at such doses, even higher doses (or effective higher dosesby a different, more localized delivery route) may be employed to theextent that patient tolerance permits. Continuous dosing over, forexample 24 hours or multiple doses per day are contemplated to achieveappropriate systemic levels of compounds.

[0071] Advantageously, the compositions are formulated as dosage units,each unit being adapted to supply a fixed dose of active ingredients.Tablets, coated tablets, capsules, ampoules and suppositories areexamples of dosage forms according to the invention.

[0072] It is only necessary that the active ingredient constitute aneffective amount, i.e., such that a suitable effective dosage will beconsistent with the dosage form employed in single or multiple unitdoses. The exact individual dosages, as well as daily dosages, aredetermined according to standard medical principles under the directionof a physician or veterinarian for use humans or animals.

[0073] The pharmaceutical compositions will generally contain from about0.0001 to 99 wt. %, preferably about 0.001 to 50 wt. %, more preferablyabout 0.01 to 10 wt. % of the active ingredient by weight of the totalcomposition. In addition to the active agent, the pharmaceuticalcompositions and medicaments can also contain other pharmaceuticallyactive compounds. Examples of other pharmaceutically active compoundsinclude, but are not limited to, analgesic agents, cytokines andtherapeutic agents in all of the major areas of clinical medicine. Whenused with other pharmaceutically active compounds, the conopeptides ofthe present invention may be delivered in the form of drug cocktails. Acocktail is a mixture of any one of the compounds useful with thisinvention with another drug or agent. In this embodiment, a commonadministration vehicle (e.g., pill, tablet, implant, pump, injectablesolution, etc.) would contain both the instant composition incombination supplementary potentiating agent. The individual drugs ofthe cocktail are each administered in therapeutically effective amounts.A therapeutically effective amount will be determined by the parametersdescribed above; but, in any event, is that amount which establishes alevel of the drugs in the area of body where the drugs are required fora period of time which is effective in attaining the desired effects.

[0074] The present invention also relates to rational drug design forthe indentification of additional drugs which can be used for thepursposes described herein. The goal of rational drug design is toproduce structural analogs of biologically active polypeptides ofinterest or of small molecules with which they interact (e.g., agonists,antagonists, inhibitors) in order to fashion drugs which are, forexample, more active or stable forms of the polypeptide, or which, e.g.,enhance or interfere with the function of a polypeptide in vivo. Severalapproaches for use in rational drug design include analysis ofthree-dimensional structure, alanine scans, molecular modeling and useof anti-id antibodies. These techniques are well known to those skilledin the art. Such techniques may include providing atomic coordinatesdefining a three-dimensional structure of a protein complex formed bysaid first polypeptide and said second polypeptide, and designing orselecting compounds capable of interfering with the interaction betweena first polypeptide and a second polypeptide based on said atomiccoordinates.

[0075] Following identification of a substance which modulates oraffects polypeptide activity, the substance may be further investigated.Furthermore, it may be manufactured and/or used in preparation, i.e.,manufacture or formulation, or a composition such as a medicament,pharmaceutical composition or drug. These may be administered toindividuals.

[0076] A substance identified as a modulator of polypeptide function maybe peptide or non-peptide in nature. Non-peptide “small molecules” areoften preferred for many in vivo pharmaceutical uses. Accordingly, amimetic or mimic of the substance (particularly if a peptide) may bedesigned for pharmaceutical use.

[0077] The designing of mimetics to a known pharmaceutically activecompound is a known approach to the development of pharmaceuticals basedon a “lead” compound. This approach might be desirable where the activecompound is difficult or expensive to synthesize or where it isunsuitable for a particular method of administration, e.g., purepeptides are unsuitable active agents for oral compositions as they tendto be quickly degraded by proteases in the alimentary canal. Mimeticdesign, synthesis and testing is generally used to avoid randomlyscreening large numbers of molecules for a target property.

[0078] Once the pharmacophore has been found, its structure is modeledaccording to its physical properties, e.g., stereochemistry, bonding,size and/or charge, using data from a range of sources, e.g.,spectroscopic techniques, x-ray diffraction data and NMR. Computationalanalysis, similarity mapping (which models the charge and/or volume of apharmacophore, rather than the bonding between atoms) and othertechniques can be used in this modeling process.

[0079] A template molecule is then selected, onto which chemical groupsthat mimic the pharmacophore can be grafted. The template molecule andthe chemical groups grafted thereon can be conveniently selected so thatthe mimetic is easy to synthesize, is likely to be pharmacologicallyacceptable, and does not degrade in vivo, while retaining the biologicalactivity of the lead compound. Alternatively, where the mimetic ispeptide-based, further stability can be achieved by cyclizing thepeptide, increasing its rigidity. The mimetic or mimetics found by thisapproach can then be screened to see whether they have the targetproperty, or to what extent it is exhibited. Further optimization ormodification can then be carried out to arrive at one or more finalmimetics for in vivo or clinical testing.

[0080] The present invention further relates to the use of a labeled(e.g., radiolabel, fluorophore, chromophore or the like) of theconotoxins described herein as a molecular tool both in vitro and invivo, for discovery of small molecules that exert their action at orpartially at the same functional site as the native toxin and capable ofelucidation similar functional responses as the native toxin. In oneembodiment, the displacement of a labeled conotoxin from its receptor orother complex by a candidate drug agent is used to identify suitablecandidate drugs. In a second embodiment, a biological assay on a testcompound to determine the therapeutic activity is conducted and comparedto the results obtained from the biological assay of a conotoxin. In athird embodiment, the binding affinity of a small molecule to thereceptor of a conotoxin is measured and compared to the binding affinityof a conotoxin to its receptor.

[0081] The practice of the present invention employs, unless otherwiseindicated, conventional techniques of chemistry, molecular biology,microbiology, recombinant DNA, genetics, immunology, cell biology, cellculture and transgenic biology, which are within the skill of the art.See, e.g., Maniatis et al., 1982; Sambrook et al., 1989; Ausubel et al.,1992; Glover, 1985; Anand, 1992; Guthrie and Fink, 1991; Harlow andLane, 1988; Jakoby and Pastan, 1979; Nucleic Acid Hybridization (B. D.Hames & S. J. Higgins eds. 1984); Transcription And Translation (B. D.Hames & S. J. Higgins eds. 1984); Culture Of Animal Cells (R. I.Freshney, Alan R. Liss, Inc., 1987); Immobilized Cells And Enzymes (IRLPress, 1986); B. Perbal, A Practical Guide To Molecular Cloning (1984);the treatise, Methods In Enzymology (Academic Press, Inc., N.Y.); GeneTransfer Vectors For Mammalian Cells (J. H. Miller and M. P. Calos eds.,1987, Cold Spring Harbor Laboratory); Methods In Enzymology, Vols. 154and 155 (Wu et al. eds.), Immunochemical Methods In Cell And MolecularBiology (Mayer and Walker, eds., Academic Press, London, 1987); HandbookOf Experimental Immunology, Volumes I-IV (D. M. Weir and C. C.Blackwell, eds., 1986); Riott, Essential Immunology, 6th Edition,Blackwell Scientific Publications, Oxford, 1988; Hogan et al.,Manipulating the Mouse Embryo, (Cold Spring Harbor Laboratory Press,Cold Spring Harbor, N.Y., 1986).

EXAMPLES

[0082] The present invention is described by reference to the followingExamples, which are offered by way of illustration and are not intendedto limit the invention in any manner. Standard techniques well known inthe art or the techniques specifically described below were utilized.

Example 1 Isolation of Conotoxin Peptides

[0083] Crude venom was extracted from venom ducts (Cruz et al., 1976),and the components were purified as previously described (Cartier etal., 1996). The crude extract from venom ducts was purified by reversephase liquid chromatography (RPLC) using a Vydac Cl₁₈ semi-preparativecolumn (10×250 mm). Further purification of bioactive peaks was done ona Vydac C₁₈ analytical column (4.6×220 mm). The effluents were monitoredat 220 nm. Peaks were collected, and aliquots were assayed for activity.Throughout purification, HPLC fractions were assayed by means ofintracerebral ventricular (i.c.v.) injection into mice (Clark et al.,1981).

[0084] The amino acid sequence of the purified peptides were determinedby standard methods. The purified peptides were reduced and alkylatedprior to sequencing by automated Edman degradation on an AppliedBiosystems 477A Protein Sequencer with a 120A Analyzer (DNA/PeptideFacility, University of Utah) (Martinez et al., 1995; Shon et al.,1994).

[0085] In accordance with this method, the conotoxin peptides describedas “isolated” in Table 1 were obtained. These conotoxin peptides, aswell as the other conotoxin peptides and the conotoxin peptideprecursors set forth in Table 1 are synthesized as described in U.S.Pat. No. 5,670,622.

EXAMPLE 2 Isolation of DNA Encoding Conopeptides

[0086] DNA coding for conotoxin peptides was isolated and cloned inaccordance with conventional techniques using general procedures wellknown in the art, such as described in Olivera et al. (1996), includingusing primers based on the DNA sequence of known conotoxin peptides. Forexample, primers based on the DNA sequence for the Contulakin-Gpropeptide were used to identify contulakin homologs. The propeptides ofthese contulakin homologs are homologous on the basis of primeramplification, even though the sequence of the mature toxins are nothomologous with the Contulakin-G mature toxin. Alternatively, cDNAlibraries was prepared from Conus venom duct using conventionaltechniques. DNA from single clones was amplified by conventionaltechniques using primers which correspond approximately to the M13universal priming site and the M13 reverse universal priming site.Clones having a size of approximately 300-500 nucleotides were sequencedand screened for similarity in sequence to known conotoxins. The DNAsequences and encoded propeptide sequences are set forth in Table 1. DNAsequences coding for the mature toxin can also be prepared on the basisof the DNA sequences set forth in Table 1. An alignment of theconopeptides of the present invention is set forth in Tables 2-14. TABLE1 Name:     Af6.1 Species:  ammiralis Cloned:   Yes DNA Sequence: (SEQID NO:1) ATCATGGAGAAACTGATAATTCTGCTTCTTGTTGCTGCTGTACTGATGTCGACCCAGGCCCTGGTTGAACGTGCTGGAGAAAACCGCTCAAAGGAGAACATCAATTTTTTATTAAAAAGAAAGAGAGCTGCTGACAGGGGGATGTGGGGCGATTGCAAAGATGGGTTAACGACATGTTTTGCGCCCTCAGAGTGTTGTTCTGAGGATTGTGAAGGGAGCTGCACGATGTGGTGATGACCTCTGACCACAAGCCATCTGACATCACCACTCTCCTCTTCAGAGGCTTCAAG Translation: (SEQ ID NO:2)MERLIILLLVAAVLMSTQALVERAGENRSKENINFLLKRKRAADRGMWGDCKDGLTTCFAPSECCSEDCEGSCTMW Toxin Sequence: (SEQ ID NO:3)Gly-Met-Xaa4-Gly-Asp-Cys-Lys-Asp-Gly-Leu-Thr-Thr-Cys-Phe-Ala-Xaa3-Ser-Xaa1-Cys-Cys-Ser-Xaa1-Asp-Cys-Xaa1-Gly-Ser-Cys-Thr-Met-Xaa4-{circumflex over ( )} Name:     Af6.2Species: ammiralis Cloned:   Yes DNA Sequence: (SEQ ID NO:4)ATCATGGAGAAACTGACAATTCTGCTTCTTGTTGCTGCTGTACTGATGTCGACCCAGGCCCTGCCTCAAGGTGGTGGAGAAAAACGCCCAAGGGAGAATATCAGATTTTTATCAAAAAGAAAGACAAATGCTGAGCGTTGGAGGGAGGGCAGTTGCACCTCTTGGTTAGCGACGTGTACGCAAGACCAGCAATGCTGTACTGATGTTTGTTACAAAAGGGACTACTGCGCCTTGTGGGATGACCGCTGACCACAAGCCATCTGACATCACCACTCTCCTGTTCAGAGTCTTCAAG Translation: (SEQ ID NO:5)MEKLTILLLVAAVLMSTQALPQGGGEKRPRLNIRFLSKRKTNAERWREGSCTSWLATCTQDQQCCTDVCYKRDYCALWDDR Toxin Sequence: (SEQ ID NO:6)Xaa4-Arg-Xaa1-Gly-Ser-Cys-Thr-Ser-Xaa4-Leu-Ala-Thr-Cys-Thr-Gln-Asp-Gln-Gln-Cys-Cys-Thr-Asp-Val-Cys-Xaa5-Lys-Arg-Xaa5-Cys-Ala-Leu-Xaa4-Asp-Asp- Arg-{circumflex over( )} Name:     Af6.3 Species:  ammiralis Cloned:   Yes DNA Sequence:(SEQ ID NO:7) ATCATGCAGAAACTGATAATTCTGCTTCTTGTTGCTGCTGTGCTGATGTCGACCCAGGCCCTGTTTCAAGAAAAACGCACAATGAAGAAGATCGATTTTTTATCAAAGGGAAAGGCAGATGCTGAGAAGCAGAGGAAGCGCAATTGCTCGGATGATTGGCAGTATTGTGAAAGTCCCAGTGACTGCTGTAGTTGGGATTGTGATGTGGTCTGCTCGGGATGAACTCTGACCACAAGTCATCCGACATCACCACTCTCCTGTTCAGAGGCTTCAAG Translation: (SEQ ID NO:8)MQKLIILLLVAAVLMSTQALFQEKRTMKKIDFLSKGKADAFKQRKRNCSDDWQYCESPSDCCSWDCDVVCSG Toxin Sequence: (SEQ ID NO:9)Asn-Cys-Ser-Asp-Asp-Xaa4-Gln-Xaa5-Cys-Xaa1-Ser-Xaa3-Ser-Asp-Cys-Cys-Ser-Xaa4-Asp-Cys-Asp-Val- Val-Cys-Ser-#Name:     Af6.4 Species:  ammiralis Cloned:   Yes DNA Sequence: (SEQ IDNO:10) ATCATGCAGAAACTGATAATCCTGCTTCTTGTTGCTGCTCTACTGTTGTCGATCCAGGCGGTAAATCAAGAAAAACACCAACGGGCAAAGATCAACTTGCTTTCAAAGAGAAAGCCACCTGCTGAGCGTTGGTGGCGGTGGGGAGGATGCATGGCTTGGTTTGGGAAATGTTCGAAGGACTCGGAATGTTGTTCTAATAGTTGTGACATAACGCGCTGCGAGTTAATGCGATTCCCACCAGACTGGTGACATCGACACTCTCCTGTTCAGAGTCTTCAAG Translation: (SEQ ID NO:11)MQRLIILLLVAALLLSIQAVNQEKIIQRAKTNLLSKRKPPAERWWRWGGCMAWFGKCSKDSECCSNSCDITRCELMRFPPDW Toxin Sequence: (SEQ ID NO:12)Xaa4-Xaa4-Arg-Xaa4-Gly-Gly-Cys-Met-Ala-Xaa4-Phe-Gly-Lys-Cys-Ser-Lys-Asp-Ser-Xaa1-Cys-Cys-Ser-Asn-Ser-Cys-Asp-Ile-Thr-Arg-Cys-Xaa1-Leu-Met-Arg-Phe-Xaa3-Xaa3-Asp-Xaa4-{circumflex over ( )} Name:     Af6.5Species:  ammiralis Cloned:   Yes DNA Sequence: (SEQ ID NO:13)ATCATGGAGAAACTGACAATCCTGCTTCTTGTTGCTGCTGTACTGACGTCGACCCAGGCCCTGATTCAAGGTGGTGGAGACGAACGCCAAAAGGCAAAGATCAACTTTCTTTCAAGGTCGGACCGCGATTGCAGGGGTTACGATGCGCCGTGTAGCTCTGGCGCGCCATGTTGTGATTGGTGGACATGTTCAGCACGAACCGGGCGCTGTTTTTAGGCTGACCACAAGCCATCCGACATCACCACTCTCC TCTTCAGAGGCTTCAAGTranslation: (SEQ ID NO:14)MEKLTILLLVAAVLTSTQALIQGGGDERQKAKINFLSRSDRDCRGYDAPC SSGAPCCDWWTCSARTGRCFToxin Sequence: (SEQ ID NO:15)Asp-Cys-Arg-Gly-Xaa5-Asp-Ala-Xaa3-Cys-Ser-Ser-Gly-Ala-Xaa3-Cys-Cys-Asp-Xaa4-Xaa4-Thr-Cys-Ser-Ala-Arg-Thr-Gly-Arg-Cys-Phe-{circumflex over ( )} Name:     Af6.6Species:  ammiralis Cloned:   Yes DNA Sequence: (SEQ ID NO:16)ATCATGCAGAAACTGACAATTCTGCTTCTTGTTGCTGCTGTGCTGATGTCGACCCAGGCCGTGCTTCAAGAAAAACGCCCAAAGGAGAAGATCAAGTTTTTATCAAAGAAAAAGACAGATGCTGAGAAGCAGCAGAAGCGCCTTTGCCCGGATTACACGGAGCCTTGTTCACATGCCCATGAATGCTGTTCATGGAATTGTCATAATGGGCACTGCACGGGATGAACTCGGACCACAAGCCATCGACATCATCACTCTCCTGTTCAGAGTCTTCAAG Translation: (SEQ ID NO:17)MQKLTILLLVAAVLMSTQAVLQEKRPKEKIKFLSKKKTDAEKQQKRLCPDYTEPCSHAHECCSWNCHNGHCTG Toxin Sequence: (SEQ ID NO:18)Leu-Cys-Xaa3-Asp-Xaa5-Thr-Xaa1-Xaa3-Cys-Ser-His-Ala-His-Xaa1-Cys-Cys-Ser-Xaa4-Asn-Cys-His-Asn-Gly- His-Cys-Thr-#Name:     Af6.7 Species:  ammiralis Cloned:   Yes DNA Sequence: (SEQ IDNO:19) ATCATGCAGAAACTGATAATTCTGCTCCTTGTTGCTGCTGTACTGATGTCGACCCAGGCCATGTTTCAAGGTGATGGAGAAAAATCCCGGAAAGCGGAGATCAACTTTTCTAAAACAAGAAATTTGGCGAGAAACAAGCAGAAACGCTGCAGTAGTTGGGCAAAGTATTGTGAAGTTGACTCGGAATGCTGTTCCGAACAGTGTGTAAGGTCTTACTGCGCGATGTGGTGATGACCTCTGACCACAAGCCATCCGATATCACCACTCTCCTCTTCAGAGACTTCAAG Translation: (SEQ ID NO:20)MQKLIILLLVAAVLMSTQAMFQGDGEKSRKAFThESKTRMARINKQKRCSSWAKYCEVDSECCSEQCVRSYCAMW Toxin Sequence: (SEQ ID NO:21)Cys-Ser-Ser-Xaa4-Ala-Lys-Xaa5-Cys-Xaa1-Val-Asp-Ser-Xaa1-Cys-Cys-Ser-Xaa1-Gin-Cys-Val-Arg-Ser-Xaa5-Cys-Ala-Met-Xaa5-{circumflex over ( )} Name:     Af9.1Species:  ammiralis Cloned:   Yes DNA Sequence: (SEQ ID NO:22)GTTAAAATGCATCTGTCACTGGCACGCTCAGCTGTTTTGATGTTGCTTCTGCTGTTTGCCTTGGGCAACTTTGTTGTGGTCCAGTCAGGACAGATAACAAGAGATGTGGACAATGGACAGCTCACGGACAACCGCCGTAACCTGCAATCGAAGTGGAAGCCAGTGAGTCTCTTCATGTCACGACGGTCTTGTAACAATTCTTGCAATGAGCATTCCGATTGCGAATCCCATTGTATTTGCACGTTTAGCGGATGCAAAATTATTTTGATATAAACGGATTGAGTTTGCTCGTCAACAAGATGTCGCACTACAGCTCCTCTCTACAGTGTGTACATCGACCAAACGACGCATCTTTTATTTCTTTGTCTGTTGTATTTGTTTTCCTGTGTTCATAACGTACAGAGCCCTTTAATTACCTTTACTGCTCTTCACTTAACCTGATAACCGGAA GGTCCAGTGCTTranslation: (SEQ ID NO:23)MHLSLARSAVLMLLLLFALGNFVVVQSGQITRDVDNGQLTDNRRNLQSKWKPVSLFMSRRSCNNSCNEHSDCESHCICTFSGCKIILI Toxin Sequence: (SEQ ID NO:24)Ser-Cys-Asn-Asn-Ser-Cys-Asn-Xaa1-His-Ser-Asp-Cys-Xaa1-Ser-His-Cys-Ile-Cys-Thr-Phe-Ser-Gly-Cys-Lys-Ile-Ile-Leu-Ile-{circumflex over ( )} Name:     Af9.2Species:  ammiralis Cloned:   Yes DNA Sequence: (SEQ ID NO:25)GTTAAAATGCATCTGTCACTGGCACGCTTAGCTGTTTTGATGTTGCTTCTGCTGTTTGCCTTGGGCAACTTTGTTGTGGTCCAGTCAGGACAGATAACAAGAGATGTGGACAATGGACAGCTCACGGACAACCGCCGTAACCTGCAATCGAAGTGGAAGCCAGTGAGTCTCTTCATGTCACGACGGTCTTGTAACAATTCTTGCAATGAGCATTCCGATTGCGAATCCCATTGTATTTGCACGTTTAGAGGATGCGGAGCTGTTAATGGTTGAGTTTGCTCGTCAACATGATGTCGCACTACACACTACAGCTCCTCTCTACAGTGTGTACATCGACCAAACGACGCATCTTTTATTTCTTTGTCTGTTGTGTTTGTTTTCCTGTGTTCATAACGTACAGAGCCCTTTAATTACTTTTACTGCTCTTCACTTAACCTGATAACCAGAAGG TCCAGTGCTTranslation: (SEQ ID NO:26)MHLSLARLAVLMLLLLFALGNFVVVQSGQITRDVDNGQLTDNRRINLQSKWRPVSLFMSRRSCNNSCKEHSDCESHCICTFRGCGAVNG Toxin Sequence: (SEQ ID NO:27)Ser-Cys-Asn-Asn-Ser-Cys-Asn-Xaa1-His-Ser-Asp-Cys-Xaa1-Ser-His-Cys-Ile-Cys-Thr-Phe-Arg-Gly-Cys-Gly- Ala-Val-Asn-#Name:     Ar6.1 Species:  arenatus Cloned:   Yes DNA Sequence: (SEQ IDNO:28) ACCAAAACCATCATCAAAATGAAACTGACGTGCGTGGTGATCGTCGCTGTGCTGTTCCTGACGGCCTGTCAACTCACTACAGCTGATGACTCCAGAGGTACGCAGAAGCATGGTGCCCTGAGATCGACCACCAAACTCTCCATGTTGACTCGGGGCTGCACGCCTCCTGGTGGAGTTTGTGGTTATCATGGTCACTGCTGCGATTTTTGCGATACGTTCGGCAATTTATGTGTGAGTGGCTGACCCGGCATCTGACCTTTCCCCCTTCTTTGCTCCACTATCCTTTTTCTGCCTGAGTCCTCCATACCTGAGAGCTGTCATGAACCACTCAACACCTACTCTTCCGGAGGTTTCTGAGGAGCTGCATTGAAATAAAAGCCGCATTGC Translation: (SEQ ID NO:29)MKLTCVVIVAVLFLTACQLTTADDSRGTQKHGALRSTTKLSMLTRGCTPPGGVCGYHGHCCDFCDTFGNLCVSG Toxin Sequence: (SEQ ID NO:30)Gly-Cys-Thr-Xaa3-Xaa3-Gly-Gly-Val-Cys-Gly-Xaa5-His-Gly-His-Cys-Cys-Asp-Phe-Cys-Asp-Thr-Phe-Gly- Asn-Leu-Cys-Val-Ser-#Name:     Bromosleeper-Ari Species:  arenatus Cloned:   Yes DNASequence: (SEQ ID NO:31)GACAGGATTGAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTAACCCTTCTACTTCTTGTGTTCATGGCAACCAGTCATCAGGATGCAGGAGAGAAGAAGGCGATGCAAAGGGACGCAATCAACGTCAGACGGAGAAGATCACTCACTCGGGGAGTAGTAACTGAGGCGTGCGAAGAGTCCTGTGAGGAGGAGGAAAAGCACTGCTGCCACGTAAATAATGGAGTACCCTCTTGTGCCGTTATATGCTGGGGATAGTTTCTCGCACACTGTCTCATTCATTATTTTATCAGTACAAGTGTAAACGAGACATGTCAGAAAGTCGAAGGTTGTGCGTATTTGATAAGTATTGTTTACTGGGATGAACGGA Translation: (SEQ ID NO:32)MSGLGIMVLTLLLLVFMATSHQDAGEKKAMQRDAINVRRRRSLTRGVVTEACEESCEEEEKHCCHVNNGVPSCAVICWG Toxin Sequence: (SEQ ID NO:33)Val-Val-Thr-Xaa1-Ala-Cys-Xaa1-Xaa1-Ser-Cys-Xaa1-Xaa1-Xaa1-Xaa1-Lys-His-Cys-Cys-His-Val-Asn-Asn-Gly-Val-Xaa3-Ser-Cys-Ala-Val-Ile-Cys-Xaa4-# Name:     Bromosleeper-Ari ASpecies:  arenatus Cloned:   Yes DNA Sequence: (SEQ ID NO:34)GACAGGATTGAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTAACCCTTCTACTTCTTGTGTTCATGGCAACCAGTCATCAGGATGCAGGAGAGAAGCAGGCGACGGAAAGGGACGCAATCAACATCAGATGGAGAAGATCACGCACTCGGAGAATAGTAACTGAGGCGTGCGAAGAGTCCTGTGAGGACGAGGAAAAGCACTGCTGCCACGTAAATAATGGAGTACCCTCTTGTGCCGTTATATGCTGGGGATAGTTTCTCGCACACTGTCTCATTCATTATTTTATCAGTACAAGTGTAAACGAGACATGTCAGAAAGTCGAAGGTTGTGCGTATTTGATAAGTATTGTTTACTGGGATGAACGGA Translation: (SEQ ID NO:35)MSGLGIMVLTLLLLVFMATSHQDAGEKQATERDAINIRWRRSRTRRIVTEACEESCEDEEKIICCHYNNGVPSCAVICWG Toxin Sequence: (SEQ ID NO:36)Ile-Val-Thr-Xaa1-Ala-Cys-Xaa1-Xaa1-Ser-Cys-Xaa1-Asp-Xaa1-Xaa1-Lys-His-Cys-Cys-His-Val-Asn-Asn-Gly-Val-Xaa3-Ser-Cys-Ala-Val-Ile-Cys-Xaa4-# Name:     Bromosleeper-Ar2Species:  arenatus Cloned:   Yes DNA Sequence: (SEQ ID NO:37)GACAGGATTGAACAAAATTCAGGATGTCAGAACTGGGAATCATGGTGCTAACGCTTCTACTTCTTGTGTTCCTGGTAACCAGTCATCAGGATGCAGGAGAGAAGCAGGCGACGGAAAGGGACGCAATCAACATCAGATGGAGAAGATCACTCACTCGGAGAATAGTAACTGAGGCGTGCGAAGAGCACTGTGAGGATGAGGAACAGTTCTGCTGCGGCTTAGAGAATGGACAACCCTTTTGTGCCCCTGTTTGCTTCGGATAGTTTCTGTACACTGTCTCATTAATTATTTTATCAGTACAAGTGTAAACAAAACATGTCAGAAAGTCGAAGGTTGTGCGTATTTGATAAGTATTGTTTGCTGGGACGAACGGA Translation: (SEQ ID NO:38)MSELGIMVLTLLLLVFLVTSHQDAGEKQATERDAINIRWRRSLTRRIVTEACEEHCEDEEQFCCGLENGQPFCAPVCFG Toxin Sequence: (SEQ ID NO:39)Ile-Val-Thr-Xaa1-Ala-Cys-Xaa1-Xaa1-His-Cys-Xaa1-Asp-Xaa1-Xaa1-Gln-Phe-Cys-Cys-Gly-Leu-Xaa1-Asn-Gly-Gln-Xaa3-Phe-Cys-Ala-Xaa3-Val-Cys-Phe-# Name:     Bromosleeper-Ar3Species:  arenatus Cloned:   Yes DNA Sequence: (SEQ ID NO:40)GACAGGATTGAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTAACCCTTCTACTTCTTGTGTTCATGGCAACCAGTCATCAGGATGCAGGAGAGAAGAAGGTGATGCAAAGGGACGCAATCAACGTCAGACGGAGAAGATCACGCACTCGGAGAGTAGTAACTGGGGCGTGCGAAGAGCACTGTGAGGACGAGGAAAAGCACTGCTGCGGCTTAGAGAATGGACAACCCTTTTGTGCCCGTCTATGCTTAGGATAGTTTTCTGTACACTGTCTTATTCATTATTTTATCAGTACAAGTGAAAACAAAGCATGTCAGAAAGTCGAAGGTTGTGCGTATTTGATAAGTATTGTTTACTGGGATGAACGGA Translation: (SEQ ID NO:41)MSGLGIMVLTLLLLVFMATSHQDAGEKKVMQRDAINVRRRRSRTRRVVTGACEEHCEDEEKIHCCGLENGQPFCARLCLG Toxin Sequence: (SEQ ID NO:42)Val-Val-Thr-Gly-Ala-Cys-Xaa1-Xaa1-His-Cys-Xaa1-Asp-Xaa1-Xaa1-Lys-His-Cys-Cys-Gly-Leu-Xaa1-Asn-Gly-Gln-Xaa3-Phe-Cys-Ala-Arg-Leu-Cys-Leu-# Name:     C. arenatuscontryphan 1 Species:  arenatus Cloned:   Yes DNA Sequence: (SEQ IDNO:43) ATGGGGAAACTGACAATACTGGTTCTTGTTGCTGCTGTACTGTTGTCGACCCAGGTCATGGTTCAAGGTGACGGAGATCAACCTGCAGCTCGCAATGCAGTGCCAAAAGACGATAACCCAGATGGAGCGAGTGGAAAGTTCATGAATGTTCTACGTCGGTCTGGATGTCCGTGGCATCCTTGGTGTGGCTGATCGGAATC CACGATTGCAATGACAGCCTranslation: (SEQ ID NO:44)MGRLTILVLVAAVLLSTQVMVQGDGDQPAARNAVPKDDNPDGASGKFMNV LRRSGCPWHPWCG ToxinSequence: (SEQ ID NO:45) Ser-Gly-Cys-Xaa3-Xaa4-His-Xaa3-Xaa4-Cys-#Name:     C. arenatus contryphan 1A Species:  arenatus Cloned:   Yes DNASequence: (SEQ ID NO:46)ATGGGGAAACTGACAATACTGGTTCTTGTTGCTGCTGTACTGTTGTCGACCCAGGTCATGGTTCAAGGTGACGGAGATCAACCTGCAGCTCGCAATGCAGTGCCAAAAGACGATAACCCAGATGGAGCGAGTGGAAAGTTCATGAATGTTCTACGTCGGTCTGGATGTCCGTGGCGCCCTTGGTGTGGCTGATCGGAATC CACGATTGCAATGACAGCCTranslation: (SEQ ID NO:47)MGKLTILVLVAAVLLSTQVMVQGDGDQPAARNAVPKDDNPDGASGKIFMN VLRRSGCPWRPWCG ToxinSequence: (SEQ ID NO:48) Ala-Ser-Gly-Cys-Xaa3-Xaa4-Arg-Xaa3-Xaa4-Cys-#Name:     C. arenatus contryphan 2 Species:  arenatus Cloned:   Yes DNASequence: (SEQ ID NO:49)ATGGGGAAACTGACAATACTGGTTCTTGTTGCTGCTGTACTGTTGTCGACCCAGGTCATGGTTCAAGGTGACGGAGATCAACCTGCAGGTCGAGATGCAGTTCCAAGAGACGATAACCCAGGTGGAACGAGTGGAAAGTTCATGAATGCTCTACGTCAATATGGATGTCCGGTGGGTCTTTGGTGTGACTGATCAGAATC CACGATTGCAATGACAGCCTranslation: (SEQ ID NO:50)MGKLTILVLVAAVLLSTQVMVQGDGDQPAGRDAVPRDDNPGGTSGKFMNA LRQYGCPVGLWCD ToxinSequence: (SEQ ID NO:51)Xaa2-Xaa5-Gly-Cys-Xaa3-Val-Gly-Leu-Xaa4-Cys-Asp-{circumflex over ( )}Name:     C. arenatus contryphan 4 Species:  arenatus Cloned:   Yes DNASequence: (SEQ ID NO:52)ATGGGGAAACTGACAATACTGGTTCTTGTTGCTGCTGTACTGTTGTCGACCCAGGTCATGTTTCGAGATCAACCTGCACGTCGTGATGCAGTGCCAAGAGACGATAGCCCAGATGGAATGAGTGGAGGGTTCATGAATGTCCCACGTCGGTCTGGATGTCCGTGGCAACCTTGGTGTGGCTGATCGGAATCCACGATTGC AATGACAGCCTranslation: (SEQ ID NO:53)MGKLTILVLVAAVLLSTQVMFRDQPARRDAVPRDDSPDGMSGGFMNVPRR SGCPWQPWCG ToxinSequence: (SEQ ID NO:54) Ser-Gly-Cys-Xaa3-Xaa4-Gln-Xaa3-Xaa4-CyS-#Name:     Contryphan-Ar-1 Species:  arenatus Cloned:   Yes DNA Sequence:(SEQ ID NO:55) ATGGGGAAACTGACAATACTGGTTCTTGTTGCTGCTGTACTGTTGTCGACCCAGGCCATGGTTCAAGATCAACCTGCAGGTCGAGATGCAGTTCCAAGAGACGATAACCCAGGTGGAACGAGTGGAAAGTTCGTGAATGCTCAACGTCAATATGGATGTCCGCCGGGTCTTTGGTGTCACTGATCAGAATCCACGATTGC AATGACAGCCTranslation: (SEQ ID NO:56)MGKLTILVLVAAVLLSTQAMVQDQPAGRDAVPRDDNPGGTSGKFVNAQRQ YGCPPGLWCH ToxinSequence: (SEQ ID NO:57)Xaa2-Xaa5-Gly-Cys-Xaa3-Xaa3-Gly-Leu-Xaa4-Cys-His-{circumflex over ( )}Name:     A10.1 Species:  aurisiacus Cloned:   Yes DNA Sequence: (SEQ IDNO:58) ATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCATCCCTTCAGATCGTGCATCTGATGGCAGGAATGCCGCAGTCAACGAGAGAGCGCCTTGGCTGGTCCCTTCGACAATCACGACTTGCTGTGGATATAATCCGGGGACAATGTGCCCTCCTTGCAGGTGCGATAATACCTGTTAACCAAAAAA AAAAAAAAAAAAATranslation: (SEQ ID NO:59)MFTVFLLVVLATTVVSIPSDRASDGRNAAVNERNPWLVPSTITTCCGYNP GTMCPPCRCDNTC ToxinSequence: (SEQ ID NO:60) Ala-Xaa3-Xaa4-Leu-Val-Xaa3-Ser-Thr-Ile-Thr-Thr-Cys-Cys-Gly-Xaa5-Asn-Xaa3-Gly-Thr-Met-Cys-Xaa3-Xaa3-Cys-Arg-Cys-Asp-Asn-Thr-Cys-{circumflex over ( )} Name:     Bnl.5Species:  bandanus Cloned:   Yes DNA Sequence: (SEQ ID NO:61)ATGCGCTGTCTCCCAGTCTTGATCATTCTTCTGCTGCTGACTGCATCTGCACCTGGCGTTGATGTCCTACCGAAGACCGAAGATGATGTGCCCCTGTCATCTGTCTACGATAATACAAAGAGTATCCTACGAGGACTTCTGGACAAACGTGCTTGCTGTGGCTACAAGCTTTGCTCACCATGTTAACCAGCATGAAGGAT CC Translation: (SEQID NO:62) MRCLPVLIILLLLTASAPGVDVLPKTEDDVPLSSVYDNTKSILRGLLDKR ACCGYKLCSPCToxin Sequence: (SEQ ID NO:63)Ala-Cys-Cys-Gly-Xaa5-Lys-Leu-Cys-Ser-Xaa3-Cys-{circumflex over ( )}Name:     Ca6.3 Species:  caracteristicus Cloned:   Yes DNA Sequence:(SEQ ID NO:64) GGATCCATGAAACTGACGTGCGTGGTGATCATCGCCGCGCTGTTCCTGACGGCCTGTCAGCTCAATACAGCTGATGACTCCAGAGATAAGCAGGAGTACCGTGCAGTGAGGTTGAGAGACGGAATGCGGAATTTCAAAGGTTCCAAGCGCAACTGCGGGGAACAAGGTGAAGGTTGTGCTACTCGCCCATGCTGCTCTGGTCTGAGTTGCGTTGGCAGCCGTCCAGGAGGCCTGTGCCAGTACGGCTAATAGTCTGGCATCTGATATTTCCCCTCTGCACTCTACCTTCTTTTGCCTGATGCATGTTTACTTGTGTGTGGTCATGAACCACTCAGTAGCTACACCTCCGA AGGACGTGCTranslation: (SEQ ID NO:65)MKLTCVVHAALFLTACQLNTADDSRDKQEYRAVRLRDGMRNFKGSKRNCGEQGEGCATRPCCSGLSCVGSRPGGLCQYG Toxin Sequence: (SEQ ID NO:66)Asn-Cys-Gly-Xaa1-Gln-Gly-Xaa1-Gly-Cys-Ala-Thr-Arg-Xaa3-Cys-Cys-Ser-Gly-Leu-Ser-Cys-Val-Gly-Ser-Arg-Xaa3-Gly-Gly-Leu-Cys-Gln-Xaa5-# Name:     Ca8.1Species:  caracteristicus Cloned:   Yes DNA Sequence: (SEQ ID NO:67)ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCATCCTGCCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGAAAAACGCACCTGAAGAGAGGCTTCTACGGTACTCTGGCAATGTCTACCAGAGGATGCTCTGGCACTTGCCATCGTCGTGAGGACGGCAAGTGTCGGGGTACTTGCGACTGCTCCGGATACAGCTATTGTCGCTGCGGTGACGCTCACCATTTTTACCGAGGATGCACGTGTTCGTGTCAAGGTTGATTAATTGACTCTTTTAACTCGTTGAACGATTGAAAAAAAAAATTTTAGAGCAATATGTTCGAGAAAAACCGAAGAC Translation: (SEQ IDNO:68) MMSRMGAMFVLLLLFILPSSQQEGDVQARIKTHLKRGFYGTLAMSTRGCSGTCHRREDGKCRGTCDCSGYSYCRCGDAHHFYRGCTCSCQG Toxin Sequence: (SEQ ID NO:69)Gly-Cys-Ser-Gly-Thr-Cys-His-Arg-Arg-Xaa1-Asp-Gly-Lys-Cys-Arg-Gly-Thr-Cys-Asp-Cys-Ser-Gly-Xaa5-Ser-Xaa5-Cys-Arg-Cys-Gly-Asp-A1a-His-HiS-Phe-Xaa5-Arg-Gly-Cys-Thr-Cys-Ser-Cys-Gln-# Name:     Ca8.2 Species:  caracteristicusCloned:   Yes DNA Sequence: (SEQ ID NO:70)ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCATCCTGCCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGAAAAACGCACCGGAAGAGCGGCTTCTACGGTACTCTGGCAATGTCTGCCAGAGGATGCTCTGGCACTTGCCATCGTCGTGAGGACGGCAAGTGTCGGGGTACTTGCGACTGCTCCGGATACAGCTATTGTCGCTGCGGTGACGCTCACCATTTTTACCGAGGATGCACGTGTACATGTTAAGGTTGATTAATTGACTCTTTTAACTCGTTGAACCGATTAAAAAAAAATTAGACGAATATGTTCGAGAAAACCGAAGAC Translation: (SEQ IDNO:71) MMSKMGAMFVLLLLFILPSSQQEGDVQARKTHIRKSGFYGTLAMSARGCSGTCHRREDGKCRGTCDCSGYSYCRCGDAHHFYRGCTCTC Toxin Sequence: (SEQ ID NO:72)Gly-Cys-Ser-Gly-Thr-Cys-His-Arg-Arg-Xaa1-Asp-Gly-Lys-Cys-Arg-Gly-Thr-Cys-Asp-Cys-Ser-Gly-Xaa5-Ser-Xaa5-Cys-Arg-Cys-Gly-Asp-Ala-His-His-Phe-Xaa5-Arg-Gly-Cys-Thr-Cys-Thr-Cys-{circumflex over ( )} Name:     Ca8.3Species:  caracteristicus Cloned:   Yes DNA Sequence: (SEQ ID NO:73)ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCATCCTGCCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGAAAAACGCACCGGAAGAGCGGCTTCTACGGTACTCTGGCAATGTCTACCAGAGGATGCTCTGGCACTTGCCGTCGTCATCGGGACGGCAAGTGTCGGGGTACTTGCGACTGCTCCGGATACAGCTATTGTCGCTGCGGTGACGCTCACCATTTTTACCGAGGATGCACGTGTACATGTTAAGGTTGATTAATTCGATCTTTTAACTCGTTGAACGATTAAAAAAAAAATTTTAGACGAATATGTTCGAGAAAAACCGAAGAC Translation: (SEQ IDNO:74) MMSKMGAMFVLLLLFILPSSQQEGDVQARKTHRKSGFYGTLAMSTRGCSGTCRRHRDGKCRGTCDCSGYSYCRCGDAHHFYRGCTCTC Toxin Sequence: (SEQ ID NO:75)Gly-Cys-Ser-Gly-Thr-Cys-Arg-Arg-His-Arg-Asp-Gly-Lys-Cys-Arg-Gly-Thr-Cys-Asp-Cys-Ser-Gly-Xaa5-Ser-Xaa5-Cys-Arg-Cys-Gly-Asp-Ala-His-His-Phe-Xaa5-Arg-Gly-Cys-Thr-Cys-Thr-Cys-{circumflex over ( )} Name:     Ca8.4Species:  caracteristicus Cloned:   Yes DNA Sequence: (SEQ ID NO:76)ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCATCCTGCCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGAAAAACGCACCTGAAGAGAGGCTTCTACGGTACTCTGGCAATGTCTACCAGAGGATGCTCTGGCACTTGCCGTCGTCATCGGGACGGCAAGTGTCGGGGTACTTGCGACTGCTCCGGATACAGCTATTGTCGCTGCGGTGACGCTCACCATTTTTACCGAGGATGCACGTGTACATGTTAAGGTTGATTAATTGACTCTTTTAACTCGTTGAACGATTAAAAAAAAAAATTTTAGAGCAATATGTTCGAGAAAAACCGAAGAC Translation: (SEQ IDNO:77) MMSKMGAMFVLLLLFILPSSQQEGDVQARKTHLKRGFYGTLAMSTRGCSGTCRRHRDGKCRGTCDCSGYSYCRCGDAHHFYRGCTCTC Toxin Sequence: (SEQ ID NO:78)Gly-Cys-Ser-Gly-Thr-Cys-Arg-Arg-His-Arg-Asp-Gly-Lys-Cys-Arg-Gly-Thr-Cys-Asp-Ser-Gly-Xaa5-Ser-Xaa5-Cys-Arg-Cys-Gly-Asp-Ala-His-His-Phe-Xaa5-Arg-Gly-Cys-Thr-Cys-Thr-Cys-{circumflex over ( )} Name:     Ca8.5Species:  caracteristicus Cloned:   Yes DNA Sequence: (SEQ ID NO:79)ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGTTTCTTTTCACCCTGCCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGAAAAACGCACCTGAAGAGAGGCTTCTACGGTACTCTGGCAATGTCTTCCAGAGGATGCTCTGGCACTTGCCATCGTCGTGAGGACGGCAAGTGTCGGGGTACTTGCGACTGCTCCGGATACAGCTATTGTCGCTGCGGTGACGCTCACCATTTTTACCGAGGATGTACGTGTACATGTTAAGGTTGATTAATTGACTCTTTTAACTCGTTGAACGATTAAAAAAAAATTTAGAGCAATATGTTCGAGAAAACCGAAGAC Translation: (SEQ IDNO:80) MMSKMGAMFVLLFLFTLPSSQQEGDVQARKTHLKRGFYGTLAMSSRGCSGTCHRREDGKCRGTCDCSGYSYCRCGDAHHFYRGCTCTC Toxin Sequence: (SEQ ID NO:81)Gly-Cys-Ser-Gly-Thr-Cys-His-Arg-Arg-Xaa1-Asp-Gly-Lys-Cys-Arg-Gly-Thr-Cys-Asp-Cys-Ser-Gly-Xaa5-Ser-Xaa5-Cys-Arg-Cys-Gly-Asp-Ala-His-His-Phe-Xaa5-Arg-Gly-Cys-Thr-Cys-Thr-Cys-{circumflex over ( )} Name:     Ca8.6Species:  caracteristicus Cloned:   Yes DNA Sequence: (SEQ ID NO:82)ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCATCCTGCCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGAAAAACGCACCTGAAGAGCGGCTTCTACGGTACTCTGGCAATGTCTGCCAGAGGATGCTCTGGCACTTGCCATCGTCGTCAAAACGGCGAGTGTCAGGGTACTTGCGACTGCGACGGACACGACCATTGTGACTGCGGTGACACTCTCGGTACTTACTCAGGATGCGTGTGTATATGTTAAGGTTGATTAATTGACTCTTTTAACTCGTTGAACGATTAAAAAAATTTAGAGCAATATGTTCGAGAAAAACCGAAGAC Translation: (SEQ ID NO:83)MMSKMGAMFVLLLLFILPSSQQEGDVQARKTHLKSGFYGTLAMSARGCSGTCHIRRQNGECQGTCDCDGHDLICDCGDTLGTYSGCVCIC Toxin Sequence: (SEQ ID NO:84)Gly-Cys-Ser-Gly-Thr-Cys-His-Arg-Arg-Gln-Asn-Gly-Xaa1-Cys-Gln-Gly-Thr-Cys-Asp-Cys-Asp-Gly-His-Asp-His-Cys-Asp-Cys-Gly-Asp-Thr-Leu-Gly-Thr-Xaa5-Ser-Gly-Cys-Val-Cys-Ile-Cys-{circumflex over ( )} Name:     Ca9.1Species:  caracteristicus Cloned:   Yes DNA Sequence: (SEQ ID NO:85)GTTACAATGCATCTGTCACTGGCACGCTCAGCTGTCTTGATGTTGCTTCTGCTGTTTGCCTTGGACAACTTCGTTGGGGTCCAGCCAGGACAGATAACAAGAGATGTGGACAACCGCCGTAACCGGCAATCGCGATGGAAGCCAAGGAGTCTCTTCAAGTCACTTCATAAACGAGCATCGTGTGGAGGGACTTGCACGGAAAGTGCCGATTGCCCTTCCACGTGTAGTACTTGCTTACATGCTCAATGCGAGTCAACATGATGTCGCACTACAGCTCTTCTCTACAGTGTGTACATCGACCGTACGACGCATCTTTTATTTCTTTGGCTGTTTCATTCGTTTTCTTGTGTTCATAACATGCGGAGCCCTTCCGTTACCTCTACTGCTCTACACTTAACCTGATAACCAGAAAATCCAGTACT Translation: (SEQ ID NO:86)MHLSLARSAVLMLLLLFALDNIWGVQPGQITRDVDNRRNRQSRWKPRSLFKSLHKRASCGGTCTESADCPSTCSTCLHAQCEST Toxin Sequence: (SEQ ID NO:87)Ala-Ser-Cys-Gly-Gly-Thr-Cys-Thr-Xaa1-Ser-Ala-Asp-Cys-Xaa3-Ser-Thr-Cys-Ser-Thr-Cys-Leu-His-Ala-Gln-Cys-Xaa1-Ser-Thr-{circumflex over ( )} Name:     Ca9.2Species:  caracteristicus Cloned:   Yes DNA Sequence: (SEQ ID NO:88)GTTACAATGCATCTGTCACTGGCACGCTCAGCTGTTTTGATGTTGCTTCTGCTGTTTGCCTTGGACAACTTCGTTGGGGTCCAACCAGGACAGATAACTAGAGATGTGGACAACCGCCGTAACCTGCAATCGCGATGGAAGCCAAGGAGTCTCTTCAAGTCACTTCATAAACGAGCATCGTGTGGAGGGACTTGCACGGAAAGTGCCGATTGCCCTTCCACGTGTAGTACTTGCTTACATGCTCAATGCGAGTGAACATGATGTCGCACTACAGCTCTTCTCTACAGTGTGTACATCGACCGACCGTACGACGCATCTTTTATTTCTTTGTCTGTTTCATTCGTTTTCTTGAGTTCATAACATGCGGAGCCCTTCCGTTACCTCTACTGCTCTACACTTAAGCTGATAACCAGAAAATCCAGTACT Translation: (SEQ ID NO:89)MHLSLARSAVLMLLLLFALDNFVGVQPGQITRDVDNRRINLQSRWKPRSLFKSLHKRASCGGTCTESADCPSTCSTCLGHAQCE Toxin Sequence: (SEQ ID NO:90)Ser-Cys-Gly-Gly-Thr-Cys-Thr-Xaa1-Ser-Ala-Asp-Cys-Xaa3-Ser-Thr-Cys-Ser-Thr-Cys-Leu-His-Ala-Gln-Cys- Xaa1-{circumflex over( )} Name:     Cr10.2 Species:  circumcisus Cloned:   Yes DNA Sequence:(SEQ ID NO:91) tgtgtgtgtgtggttctgggtccaGCATTTGATGGCAGGAATGCCGCAGTCAACGAGAGAGCGCCTTGGACGGTCGTTTTGTCCACCACGAATTGCTGCGGTTATAATACGATGGAATTCTGCCCTGCTTGCATGTGCACTTATTCCTGTCCAAAAAAGAAAAAACCAGGAAAAGGCCGCAGAAACAACTGATGCTCCAG GACCCTCTGAACCACGACGTTranslation: (SEQ ID NO:92)FDGRNAAVNERAPWTVVLSTTNCCGYNTMEFCPACMCTYSCPKKKKPGKG RRNN Toxin Sequence:(SEQ ID NO:93) Ala-Xaa3-Xaa4-Thr-Val-Val-Leu-Ser-Thr-Thr-Asn-Cys-Cys-Gly-Xaa5-Asn-Thr-Met-Xaa1-Phe-Cys-Xaa3-Ala-Cys-Met-Cys-Thr-Xaa5-Ser-Cys-Xaa3-Lys-Lys-Lys-Lys-Xaa3-Gly-Lys-Gly-Arg-Arg-Asn-Asn-{circumflex over ( )} Name:     Cn9.1Species:  consors Cloned:   Yes DNA Sequence: Translation: (SEQ IDNO:94) GIFVGVQPEQITRDVDKGYSTDDGHDLLSLLKQISLRACTGSCNSDSECY NFCDCIGTRCEAQKToxin Sequence: (SEQ ID NO:95)Ala-Cys-Thr-Gly-Ser-Cys-Asn-Ser-Asp-Ser-Xaa1-Cys-Xaa5-Asn-Phe-Cys-Asp-Cys-Ile-Gly-Thr-Arg-Cys-Xaa1-Ala-Gln-Lys-{circumflex over ( )} Name:     De6.1 Species:  delessertiiIsolated: Yes Toxin Sequence: (SEQ ID NO:96)Ala-Cys-Lys-Xaa3-Lys-Asn-Asn-Leu-Cys-Ala-Ile-Thr-Xaa1-Met-Ala-Xaa1-Cys-Cys-Ser-Gly-Phe-Cys-Leu-Ile-Xaa5-Arg-Cys-{circumflex over ( )} Name:     Bromosleeper-DilSpecies:  distans Cloned:   Yes DNA Sequence: (SEQ ID NO:97)GACAGGATTGAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTAACCCTTCTACTTCTTGTGCCCATGGCAACCAGTCAACAGGATGGAGGAGAGAAGCAGGCGATGCAAAGGGACGCAATCAACGTCGCACCAGGAACATCAATCACTCGGAGAAATGTAGATCAGGAGTGCATTGACGCCTGTCAGCTGGAGGACAAGAATTGCTGTGGCAGAACAGATGGAGAACCCAGATGTGCGAAAATCTGCCTCGGATAATTTCTGTACGCTGTCTCATTCATTATTTCATCCGTACGAGTGTAAACGAGACCTATTAGAAAGTCGAAGGTTGTGCGTAATTTGATAAGCATTGTTTGCTGGGACGAACGGA Translation: (SEQ ID NO:98)MSGLGIMVLTLLLLVPMATSQQDGGEKQAMQRDAINVAPGTSITRRNVDQECIDACQLEDKNCCGRTDGEPRCAKICLG Toxin Sequence: (SEQ ID NO:99)Asn-Val-Asp-Gln-Xaa1-Cys-Ile-Asp-Ala-Cys-Gln-Leu-Xaa1-Asp-Lys-Asn-Cys-Cys-Gly-Arg-Thr-Asp-Gly-Xaa1-Xaa3-Arg-Cys-Ala-Lys-Ile-Cys-Leu-# Name:     Bromosleeper-Di2Species:  distans Cloned:   Yes DNA Sequence: (SEQ ID NO:100)GACAGGATTGAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTAACCCTTCTACTTCTTGTGCCCATGGCAACCAGTCAACAGGATGGAGGAGAGAAGCAGGCGATGCAAAGGGACGCAATCAACGTCGCACCAGGAACATCAATCACTCGGACAGAAACAGATCAGGAGTGCATTGACATCTGTAAGCAGGAGGACAAGAAATGCTGCGGCAGATCAAATGGAGAACCCACATGTGCGAAAATCTGCCTCGGATAATTTCTGTACGCTGTCTCGTTCATTATTTCGTCAGTACGAGTTTAAACGAGACCTATTAGAAAGTCGAAGGTTCGTGCTTAATTTGATAAGCATTGTTTGCTGGGATGAACGGA Translation: (SEQ ID NO:101)MSGLGIMVLTLLLLVPMATSQQDGGEKQAMQRDAINVAPGTSITRTETDQECIDICKQEDKKCCGRSNGEPTCAKICLG Toxin Sequence: (SEQ ID NO:102)Xaa1-Thr-Asp-Gln-Xaa1-Cys-Ile-Asp-Ile-Cys-Lys-Gln-Xaa1-Asp-Lys-Lys-Cys-Cys-Gly-Arg-Ser-Asn-Gly-Xaa1-Xaa3-Thr-Cys-Ala-Lys-Ile-Cys-Leu-# Name:     Bromosleeper-Di3Species:  distans Cloned:   Yes DNA Sequence: (SEQ ID NO:103)GACAGGATTGAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTAACCCTTCTACTTCTTGTGCCCATGGCAACCAGTCAACAGGATGGAGGAGAGAAGCAGGCGATGCAAAGGGACGCAATCAACGTCGCACCAGGAACATCAATCACTCGGAGAGAAACAGATCAGGAGTGCATTGACACCTGTGAGCAGGAGGACAAGAAATGCTGCGGCAGAACAAATGGAGAACCCGTATGTGCGAAAATCTGCTTCGGATAATTTCTGTACGCTGTCTCATTCATAATTTCATCAGTACGAGTTTAAACGAGACCTATTAGAAAGTCGAAGGTTCGTGCTTAATTTGATAAGCATTGTTTGCTGGGATGAACGGA Translation: (SEQ ID NO:104)MSGLGIMVLTLLLLVPMATSQQDGGEKQAMQRDAINVAPGTSITRRETDQECIDTCEQEDKKCCGRTNGEPVCAKICFG Toxin Sequence: (SEQ ID NO:105)Xaa1-Thr-Asp-Gln-Xaa1-Cys-Ile-Asp-Thr-Cys-Xaa1-Gln-Xaa1-Asp-Lys-Lys-Cys-Cys-Gly-Arg-Thr-Asn-Gly-Xaa1-Xaa3-Val-Cys-Ala-Lys-Ils-Cys-Phe-# Name:     αA-EIVBSpecies:  ermineus Isolated: Yes Cloned:   Yes DNA Sequence: (SEQ IDNO:106) ATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCTTCACTTCAGATCGTGCATCGGATGACAGGAATACCAACGACAAAGCATCTCGCCTGCTCTCTCACGTTGTCAGGGGATGCTGTGGTAAGTATCCCAATGCTGCCTGTCATCCTTGCGGTTGTACAGTGGGTAGGCCACCGTATTGTGACAGACCCAGTGGTGGAGGACGCTGATGCTCCAGGACCCTCTGAACCACGACGT Translation: (SEQ IDNO:107) MFTVFLLVVLATTVVSFTSDRASDDRNTNDKASRLLSHVVRGCCGKYPNAACHPCGCTVGRPPYCDRPSGGGR Toxin Sequence: (SEQ ID NO:108)Gly-Cys-Cys-Gly-Lys-Xaa5-Xaa3-Asn-Ala-Ala-Cys-His-Xaa3-Cys-Gly-Cys-Thr-Val-Gly-Arg-Xaa3-Xaa3-Xaa5-Cys-Asp-Arg-Xaa3-Ser-Gly-Gly-# Name:     Ge3.1 Species:  generalisCloned:   Yes DNA Sequence: (SEQ ID NO:109)GGATCCATGATGTCTAAACTGGGAGTCTTGTTGACCATCTGTCTGGTTCTGTTTCCCCTTACTGCTCTTCCACTGGATGGAGAACAACCTGTAGACCGACATGCCGAGCATATGCAGGATGACAATTCAGCTGCACAGAACCCCTGGGTTATTGCCATCAGACAGTGTTGCACGTTCTGCAACTTTGGATGCCAGCCTTGTTGCGTCCCCTGATAACGTGTTGATGACCAACTTTCTCGAG Translation: (SEQ ID NO:110)GSMMSKLGVLLTICLVLFPLTALPLDGEQPVDRHAEKMQDDNSAAQNPWV IAIRQCCTFCNFGCQPCCVPToxin Sequence: (SEQ ID NO:111)Xaa2-Cys-Cys-Thr-Phe-Cys-Asn-Phe-Gly-Cys-Gln-Xaa3-Cys-Cys-Val-Xaa3-{circumflex over ( )} Name:     C. geographus GS-ASpecies:  geographus Cloned:   Yes DNA Sequence: (SEQ ID NO:112)GCAAGATCATCAGCAGAATGAACCTGACGTGCGTGTTGATCATCGCCGTGCTCTTTCTGACGGCCTGCCAGCTCATTGCAGCTGATGACTCCAGAGATAACCAGAAGCACCGTGCAGTGAGGATGAGAGACGCATTGAAGAATTTCAAAGATTCCAGGGCGTGCTCCGGTAGAGGTTCTAGATGTCCTCCCCAATGCTGCATGGGTTTGACGTGCGGTCGTGAGTATCCACCCAGATGCGGTTGATATACGGTGAACAACTGATATTTCCCCTCTGTGCTCTACCCTCTTTTGCCTGATTCACCCACACCTATGTGTGGTCATGAACCACTCAGTACCTACACCTCTGGTGGCTTCAGAGGACGTATATTAAAATAAAACCACATTGCAATGAAAAAAAA AAAA Translation:(SEQ ID NO:113) MNLTCVLIIAVLFLTACQLIAADDSRDNQKHRAYRMRDALKNFKDSRACSGRGSRCPPQCCMGLTCGREYPPRCG Toxin Sequence: (SEQ ID NO:114)Ala-Cys-Ser-Gly-Arg-Gly-Ser-Arg-Cys-Xaa3-Xaa3-Gln-Cys-Cys-Met-Gly-Leu-Thr-Cys-Gly-Arg-Xaa1-Xaa5- Xaa3-Xaa3-Arg-Cys-# Name:Conopressin-G Species:  geographus Isolated: Yes Toxin Sequence:Cys-Phe-Ile-Arg-Asn-Cys-Xaa3-Lys-Gly-#(SEQ ID NO:115) Name:     EST66Species:  geographus Cloned:   Yes DNA Sequence: (SEQ ID NO:116)TGCTGCCCGAGTAGCAAAGAGGATTCCCTGAACTGCATTGAGACCATGGCGACCACGGCCACGTGCATGAAGTCCAACAAGGGGGAGATCTACTCCTATGCGTGCGGCTACTGCGGCAAGAAGAAGGAGAGCTGTTTCGGCGACAAAAAGCCAGTGACTGACTACCAGTGCCAGACGCGGAACATTCCCAACCCCTGCGGCGGCGCTGCTCTCTGAAGGCACCAACAGCACCAACAGCACGATCTCCTGTGTTTCGTCACTGCATTTATGACGTCAAAACCACGTCATGCATGATGACGACGATCTCGGCTATGGCATGTATTGAAGAATGGAAATAAACCTAGTTTTCA GCTGAAAAAATranslation: (SEQ ID NO:117)CCPSSKEDSLNCIETMATTATCMKSMQGEIYSYACGYCGKKKQESCFGDK KPVTDYQCQTRMPNPCGGAALToxin Sequence: (SEQ ID NO:118)Cys-Cys-Xaa3-Ser-Ser-Lys-Xaa1-Asp-Ser-Leu-Asn-Cys-Ile-Xaa1-Thr-Met-Ala-Thr-Thr-Ala-Thr-Cys-Met-Lys-Ser-Asn-Lys-Gly-Xaa1-Ile-Xaa5-Ser-Xaa5-Ala-Cys-Gly-Xaa5-Cys-Gly-Lys-Lys-Lys-Xaa1-Ser-Cys-Phe-Gly-Asp-Lys-Lys-Xaa3-Val-Thr-Asp-Xaa5-Gln-Cys-Gln-Thr-Arg-Asn-Ile-Xaa3-Asn-Xaa3-Cys-Gly-Gly-Ala-Ala-Leu- {circumflex over ( )}Name:     EST87 Species:  geographus Cloned:   Yes DNA Sequence: (SEQ IDNO:119) CGGGCGCTGCATTCCGGACGTGAAACAGCATCGCCAGCAAGTGGGCATAGTGCAAGACACtCAGAACAAtGACGCACAtAGTCTGANAAAATAACCATGGGTATGCGGATGANGTTTAGTGTGTTTCNGCAGGTTGTCNTGGGNACCACTGTCGTTTCCTTCACNTCACGTCGTGGTCCAAAATCTCGTCGCGGGGAACCTATTCCGACCACTGTAATCAACTACGGGGAGTGCTGTAAGGATCCATCCTGTTGGGTTAAGGTGAAGGATTTCCAGTGTCCTGGAGCAAGTCCTCCCAACTGAACCACGACATGTCGCCCTCTGCCTGACCTGCTTCACGTTCCGTCTCTTTCTGCCACTAGAACTCAACAACTCGATCCAACAGACTCCTACTTTACCTCCGTATTCTGAAACTACTTGGATTTGATTGTCTTTAATATCTACTCACACTTGCTGTTATTACATCATCCAAAATTTAACAAGAACATGAAAGGTGTCTGTTCAAACAAAATCAGGCAATGACAANGGGGGAAAGTCTCCANTCTATCTGAAAACTGTCACCTGTCACTCTCTTAACCAGGTTTANAACTGANTACCACTANAGCTGTTGTNCCACATCANGATCAGNCCAATTTGTANNGTTTCCTTTGCAAAACTTTTGCCTGAAATTCTTGAAAAGAAACGCTCACAATGTTGGGAAGTGCTTTTNATTANCTGACAAINISTTGNCANCATGTTCCNTTTCANTAANTCTNAAATGNAAACCTCTGTT Translation: (SEQ ID NO:120)MGMRMMFSVFLQVVLGTTVVSFTSRRGPKSRRGEPIPTTVINYGECCKDP SCWVKVKDFQCPGASPPNToxin Sequence: (SEQ ID NO:121)Gly-Xaa1-Xaa3-Ile-Xaa3-Thr-Thr-Val-Ile-Asn-Xaa5-Gly-Xaa1-Cys-Cys-Lys-Asp-Xaa3-Ser-Cys-Xaa4-Val-Lys-Val-Lys-Asp-Phe-Gln-Cys-Xaa3-Gly-Ala-Ser-Xaa3- Xaa3-Asn-{circumflexover ( )} Name:     G12.1 Species:  geographus Cloned:   Yes DNASequence: (SEQ ID NO:122)AGCCTTGATACAGAGCTGGTATCTGCTGTTAATACTTGAAAGAACAAGTGCTGTGAGCCTTCATCTCTCTCTGACTTTAGTTTGGGTCCTGGAGAAAACCTTGACGGGCAGTATGAAAATTTACCTGTGTCTTGCTTTTGTTCTGCTCCTGGCTTCTACCATAGTTGATTCAGGGCTTCTTGATAAAATTGAGACTATAAGAAACTGGAAACGCGATGACAGCTATTGTGATGGATGCCTATGCACCATATTAAAAAAAGAGACTTGCACATCGACTATGAGCTGCAGGGGAACATGCCGAAAAGAGTGGCCATGTTGGGAAGAAGACTGCTACTGTACTGAAATCCAAGGTGGAGCTTGCGTCACACCCTCAGAATGCAAACCTGGAGAGTGTTGAGGATTGGAGTGGCCAGTTCCAGCACATACAGCACCATGGTGCCCTGGACAATCGTCTATTGAATTGAATATGCCTGTGGCAGGAATCTGTCCTACAAAATAAA AAAATCATAAGTTAAAAAATranslation: (SEQ ID NO:123)MKIYLCLAFVLLLASTIVDSGLLDKIETIRNWKRDDSYCDGCLCTILKKETCTSTMSCRGTCRKEWPCWEEDCYCTEIQGGACVTPSECKPGEC Toxin Sequence: (SEQ IDNO:124) Asp-Asp-Ser-Xaa5-Cys-Asp-Gly-Cys-Leu-Cys-Thr-Ile-Leu-Lys-Lys-Xaa1-Thr-Cys-Thr-Ser-Thr-Met-Ser-Cys-Arg-Gly-Thr-Cys-Arg-Lys-Xaa1-Xaa4-Xaa3-Cys-Xaa4-Xaa1-Xaa1-Asp-Cys-Xaa5-Cys-Thr-Xaa1-Ile-Gln-Gly-Gly-Ala-Cys-Val-Thr-Xaa3-Ser-Xaa1-Cys-Lys-Xaa3- Gly-Xaa1-Cys-{circumflexover ( )} Name:     G12.2 Species:  geographus Cloned:   Yes DNASequence: (SEQ ID NO:125)AACGTTGACGGGCAGTATGAACATTTACCTGTGTCTTGCTTTTCTTCTGTTCCTGCCTTCTACCATAGTTGATTCAGGGCTTCTTGATAAAATTGAGACAATAAGGAATTGGAGACGTGATGAAAGCAAGTGTGATCGATGCAATTGCGCCGAATTAAGATCATCCAGATGCACACAAGCTATCTTCTGCCTTACACCGGAGTTATGCACACCGAGCATCTCATGTCCGACAGGTGAATGCCGCTGTACTAAGTTCCATCAGTCAAGATGCACTAGATTCGTAGAATGCGTACCTAATAAGTGTAGAGACGCATAGAGGCCAGTTCCAGCACATACAGCACCATGATGCCCTGGACAATCGTGTTGTTGGATTGAATATGCCCGTGGCAGGAATCTGTCC TACAAAAAATranslation: (SEQ ID NO:126)MNIYLCLAFLLFLPSTIVDSGLLDKIETIRINWRRDESKCDRCNCAELRSSRCTQAIFCLTPELCTPSISCPTGECRCTKFHQSRCTRFVECVPNKCRDA Toxin Sequence: (SEQID NO:127) Asp-Xaa1-Ser-Lys-Cys-Asp-Arg-Cys-Asn-Cys-Ala-Xaa1-Leu-Arg-Ser-Ser-Arg-Cys-Thr-Gln-Ala-Ile-Phe-Cys-Leu-Thr-Xaa3-Xaa1-Leu-Cys-Thr-Xaa3-Ser-Ile-Ser-Cys-Xaa3-Thr-Gly-Xaa1-Cys-Arg-Cys-Thr-Lys-Phe-His-Gln-Ser-Arg-Cys-Thr-Arg-Phe-Val-Xaa1-Cys-Val-Xaa3-Asn-Lys-Cys-Arg-Asp-Ala-{circumflex over ( )} Name:     Scratching,convulsion Species:  geographus Isolated: Yes Toxin Sequence: (SEQ IDNO:128) Lys-Phe-Leu-Ser-Gly-Gly-Phe-Lys-Xaa1-Ile-Val-Cys-His-Arg-Xaa5-Cys-Ala-Lys-Gly-Ile-Ala-Lys-Xaa1-Phe-Cys-Asn-Cys-Xaa3-Asp-# Name:     Contryphan-Im Species:  imperialisIsolated: Yes Toxin Sequence: (SEQ ID NO:129)Xaa2-Cys-Gly-Gln-Ala-Xaa4-Cys-# Name:     Im9.1 Species:  imperialisCloned:   Yes DNA Sequence: (SEQ ID NO:130)GTTAAAATGCATCTGTCACTGGCAAGCTCAGCTGCTTTGATGTTGCTTCTGCTTTTTGCCTTGGGCAACTTCGTTGGGGTCCAGCCAGGACAAATAAGAGATCTGAACAAAGGACAGCTCAAGGACAACCGCCGTAACCTGCAATCGCAGAGGAAACAAATGAGTCTCCTCAAGTCACTTCATGATCGAAATGGGTGTAACGGCAACACGTGTTCCAATAGCCCCTGCCCTAACAACTGTTATTGCGATACTGAGGACGACTGCCACCCTGACAGGCGTGAACATTAGAGATTAGAGAGTTTCCTTGTCAACATGATGTCGCACCACACCTCTGCTCTGCAGTGTGTACATCGACCAGTCGACGCATCTGTTATTTCTTTGTCTGTTGGATTGTACATCGACCAGTCCACGCATCTGTTATTTCTTTGTCTGTTTGATTTGTTTTCGTGTGTTCATAACACACAGAGCCTTTCTATTATCTGTATTGCAATACACTTTGCCTGATAACCAGAAAGTCCAGTGCT Translation: (SEQ ID NO:131)MHLSLASSAALMLLLLFALGNTVGVQPGQIRDLNKGQLKDNRRNLQSQRKQMSLLKSLHDRNGCNGNTCSNSPCPNNCYCDTEDDCHPDRREH Toxin Sequence: (SEQ IDNO:132) Asn-Gly-Cys-Asn-Gly-Asn-Thr-Cys-Ser-Asn-Ser-Xaa3-Cys-Xaa3-Asn-Asn-Cys-Xaa5-Cys-Asp-Thr-Xaa1-Asp-Asp-Cys-His-Xaa3-Asp-Arg-Arg-Xaa1-His-{circumflex over ( )}Name:     La8.1 Species:  laterculatus Cloned:   Yes DNA Sequence: (SEQID NO:133) ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCACCCTGGCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGGAAAACACACCCGAAGAGAGAGTTCCATCGTATTCTGCTAAGGCCTGACAGACAGTCCGAAACGGCTTGTAGGTCGCTCGGAAGCTACCAATGTATGGGTAAATGCCAACTCGGGGTTCATTCCTGGTGTGAATGCATTTATAACCGAGGTAGTCAGAAGTCTGGATGCGCGTGTAGGTGTCAAALAGTGATTAATTGACTCATTTAACTCGTTGAACGATTTAAAAAATCCAGAGCAATATGTTCGAGAAAAACCGAAGACGA C Translation: (SEQID NO:134) MMSKMGAMFVLLLLFTLASSQQEGDVQARKTITPKREFHRILLRPDRQSETACRSLGSYQCMGKCQLGVHSWCECIYNRGSQKSGCACRCQK Toxin Sequence: (SEQ IDNO:135) Xaa2-Ser-Xaa1-Thr-Ala-Cys-Arg-Ser-Leu-Gly-Ser-Xaa5-Gln-Cys-Met-Gly-Lys-Cys-Gln-Leu-Gly-Val-His-Ser-Xaa4-Cys-Xaa1-Cys-Ile-Xaa5-Asn-Arg-Gly-Ser-Gln-Lys-Ser-Gly-Cys-Ala-Cys-Arg-Cys-Gln-Lys-{circumflex over ( )}Name:     Lv6.2 Species:  lividus Cloned:   Yes DNA Sequence: (SEQ IDNO:136) GGATCCATGAAACTGACGTGTGTGGTGATCATCGCCGTGCTGTTCCTGACGGCCAGTCAGCTCATTACAGCTGATTACTCCAGAGATAAGCAGGAGTATCGTGCAGAGAGGCTGAGAGACGCAATGGGGAAATTCAAAGGTTCCAGGTCGTGCGGACATAGTGGTGCAGGTTGTTATACTCGCCCTTGCTGCCCTGGTCTGCATTGCTCTGGCGGCCAAGCTGGAGGCCTGTGCGTGTAATAGTAATAATCTGGCGTCTGATATTTCCAGTCTGTGCTCTACCCTCTTTTGCCTGAGTCA TCCATACCTGTGCTCGAGTranslation: (SEQ ID NO:137)MKLTCVVHAVLFLTASQLITADYSRDKQEYRAERLRDAMGKFKGSRSCGHSGAGCYTRPCCPGLHCSGGQAGGLCV Toxin Sequence: (SEQ ID NO:138)Ser-Cys-Gly-His-Ser-Gly-Ala-Gly-Cys-Xaa5-Thr-Arg-Xaa3-Cys-Cys-Xaa3-Gly-Leu-His-Cys-Ser-Gly-Gly-Gln-Ala-Gly-Gly-Leu-Cys-Val-{circumflex over ( )} Name:     Lv6.3Species:  lividus Cloned:   Yes DNA Sequence: (SEQ ID NO:139)GGATCCATGAAACTGACGTGTGTGGTGATCATATCCGTGCTGTTCCTGACGGCCAGTGAGTTCCTTACAGCTGATTACTCCAGAGATAAGCGGCAGTACCGTGCTGTGAGGTTGAGAGACGCAATGCGGAATTTCAAAGGTACCAGGGACTGCGGGGAATCAGGTCAAGGTTGCTATAGTGTACGTCCTTGCTGCCCTGGTCTGATTTGCAAAGGCACCGGTGGTGGAGGCCTGTGCCGGCCCTCTGGCATCTGATATCTCCCCTCTGTGCTCCACCCTCTTTTGCCTGAGTCATCCATA CCTGTGCTCGAGTranslation: (SEQ ID NO:140)MKLTCVVIISVLFLTASEFLTADYSRDKRQYRAVRLRDAMRNFKGTRDCGESGQGCYSVRPCCPGLICKGTGGGGLCRPSGI Toxin Sequence: (SEQ ID NO:141)Asp-Cys-Gly-Xaa1-Ser-Gly-Gln-Gly-Cys-Xaa5-Ser-Val-Arg-Xaa3-Cys-Cys-Xaa3-Gly-Leu-Ile-Cys-Lys-Gly-Thr-Gly-Gly-Gly-Leu-Cys-Arg-Xaa3-Ser-Gly-Ile-{circumflex over ( )}Name:     Convulsant Species:  magus Isolated: Yes Toxin Sequence: (SEQID NO:142) Val-Xaa5-Xaa1-Thr-His-Xaa3-{circumflex over ( )}Name:     MAG-1 Species:  magus Isolated: Yes Toxin Sequence: (SEQ IDNO:143) Arg-Xaa3-Lys-Asn-Ser-Xaa4-{circumflex over ( )} Name:     MAG-2Species:  magus Isolated: Yes Toxin Sequence: (SEQ ID NO:144)Ala-Arg-Xaa3-Lys-Asn-Ser-Xaa4-? Name:     MAG-3 Species:  magusIsolated: Yes Toxin Sequence: (SEQ ID NO:145)Arg-Xaa3-Lys-Asn-Ser-Xaa4-{circumflex over ( )} Name:     Mi6.2Species:  miles Cloned:   Yes DNA Sequence: (SEQ ID NO:146)GGATCCATGAAACTGACGTGCGTGGTGATCGTCGCCGTGCTGTTCCTGACGGCCTGTCAACTCATTACTGCTGCGAATTACGCCAGAGATGAACAGGAGTACCCCGCTGTGAGGTCGAGCGACGTGATGCAGGATTCCGAAGACTTGACGTTGACCAAGAAATGCACGGACGATTCTCAGTTCTGTAACCCTTCGAATCATGACTGCTGCAGTGGGAAGTGTATCGACGAAGGAGACAACGGCATATGCGCTATAGTCCCTGAAAACTCTTAACAATGTATACTGACATTTCCCCCTCTGTGCTCCGCCGTCCGTGGCCTGACTCGTCCATCCTTGGGCGTGGTCATGAA CCGCTCGGTTTranslation: (SEQ ID NO:147)MKLTCVVIVAVLFLTACQLITAANYARDEQEYPAVRSSDVMQDSEDLTLTKKCTDDSQFCNPSNHDCCSGKCIDEGDNGICAIVPENS Toxin Sequence: (SEQ ID NO:148)Cys-Thr-Asp-Asp-Ser-Gln-Phe-Cys-Asn-Xaa3-Ser-Asn-His-Asp-Cys-Ser-Gly-Lys-Cys-Ile-Asp-Xaa1-Gly-Asp-Asn-Gly-Ile-Cys-Ala-Ile-Val-Xaa3-Xaa1-Asn-Ser-{circumflex over ( )}Name:     Mi6.3 Species:  miles Cloned:   Yes DNA Sequence: (SEQ IDNO:149) GGATCCATGAAACTGACGTGTGTGGTGATCGTCGCCGTGCTGTTCCTGACGGCCTGTCAACTCATTACTGCTGCGAATTACGCCAGAGATGAACAGGAGTACCCTGCTGTGAGGTCGAGCGACGTGATGCAGGATTCCGAAGACCTGACGTTGACCAAGAAATGCACGGAGGATTCTCAGTTCTGTAACCCTTCGAATCATGACTGCTGCAGTGGGAAGTGTATCGACGAAGGAGACAACGGCATATGCGCTATAGTCCCTGAAAACTCTTAACAATGTATACTGACATTTCCCCCTCTGTGCTCCGCCGTCCGTGGCCTGACTCGTCCATCCTTGGGCGTGGTCATGAA CCGCTCG Translation:(SEQ ID NO:150) MKLTCVVIVAVLFLTACQLITAANYARDEQEYPAVRSSDVMQDSEDLTLTKKCTEDSQFCNPSKHDCCSGKCIDEGDNGICAIVPENS Toxin Sequence: (SEQ ID NO:151)Cys-Thr-Xaa1-Asp-Ser-Gln-Phe-Cys-Asn-Xaa3-Ser-Asn-His-Asp-Cys-Cys-Ser-Gly-Lys-Cys-Ile-Asp-Xaa1-Gly-Asp-Asn-Gly-Ile-Cys-Ala-Ile-Val-Xaa3-Xaa1-Asn-Ser- {circumflex over ( )}Name:     Mf6.1 Species:  miliaris Cloned:   Yes DNA Sequence: (SEQ IDNO:152) GGATCCATGAACTGACGTGTGTGGTGATCATCGCCGTGCTGTTCCTGACGGCCTGTCAACTCACTACAGCTGTGACTTCCTCCAGAGGTCAACAGAAGCATCGTGCTCTGAGGTCAACTGACAAAAACTCCAGGATGACCAAGCGTTGCACGCCTCCAGGTGGACTCTGTTACCATGCTTATCCCTGCTGCAGCAAGACTTGCAATCTCGATACCAGCCAATGTGAGCCTAGGTGGTCATGAACCACTCAATACCCTCTCCTCTGGAGGCTTCAGAGGAACTACATTGAAATAAAACCGCATTGCAACGAAAAAAAAAAAAAAAAAA Translation: (SEQ ID NO:153)LTCVVIIAVLFLTACQLTTAVTSSRGQQKHRALRSTDKNSRMTKRCTPPGGLCYHAYPCCSKTCNLDTSQCEPRWS Toxin Sequence: (SEQ ID NO:154)Cys-Thr-Xaa3-Xaa3-Gly-Gly-Leu-Cys-Xaa5-His-Ala-Xaa5-Xaa3-Cys-Cys-Ser-Lys-Thr-Cys-Asn-Leu-Asp-Thr-Ser-Gln-Cys-Xaa1-Xaa3-Arg-Xaa4-Ser-{circumflex over ( )}Name:     Mn10.3 Species:  monachus Cloned:   Yes DNA Sequence: (SEQ IDNO:155) tgtgtgtgtgtggttctgggtccaGCATCTGATGTCAGGAATGCCGCAGTCCACGAAAGACAGAAGGATCTGGTCGTTACGGCCACCACGACTTGCTGTGGTTATAATCCGATGACAATGTGCCCTCCTTGCATGTGCACTAATACCTGCAAAAAAAGTGGCTGATGCTCCAGGACCCTCTGAACCACGACGT Translation: (SEQ ID NO:156)SDVRNAAVHERQKDLVVTATTTCCGYNPMTMCPPCMCTNTCKKSG Toxin Sequence: (SEQ IDNO:157) Xaa2-Lys-Asp-Leu-Val-Val-Thr-Ala-Thr-Thr-Thr-Cys-Cys-Gly-Xaa5-Asn-Xaa3-Met-Thr-Met-Cys-Xaa3-Xaa3-Cys-Met-Cys-Thr-Asn-Thr-Cys-Lys-Lys-Ser-# Name:     Mn8.1Species:  monachus Cloned:   Yes DNA Sequence: (SEQ ID NO:158)ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCACCCTGGCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGGAAAACAAGCCTGAAGAGCGACTTCTATCGTGCTCTGAGAGGGTATGACAGACAGTGCACTCTTGTCAACAATTGTGACCGGAACGGTGAGCGTGCGTGTAACGGTGATTGCTCTTGCGAGGGCCAGATTTGTAAATGCGGTTATAGAGTCAGTCCTGGGAAGTCAGGATGCGCGTGTACTTGTAGAAATGCCAAATGAATCATTTAACTCGTTGAAAGATTTTTTAAAAATCCAGAGCTATATGTTCGAGAAAAACCGAAGAC Translation: (SEQ IDNO:159) MMSKMGAMFVLLLLFTLASSQQEGDVQARKTSLKSDFYRALRGYDRQCTLVNNCDRNGERACNGDCSCEGQICKCGYRVSPGKSGCACTCRNAK Toxin Sequence: (SEQ IDNO:160) Xaa2-Cys-Thr-Leu-Val-Asn-Asn-Cys-Asp-Arg-Asn-Gly-Xaa1-Arg-Ala-Cys-Asn-Gly-Asp-Cys-Ser-Cys-Xaa1-Gly-Gln-Ile-Cys-Lys-Cys-Gly-Xaa5-Arg-Val-Ser-Xaa3-Gly-Lys-Ser-Gly-Cys-Ala-Cys-Thr-Cys-Arg-Asn-Ala-Lys-{circumflex over ( )}Name:     Pn1.3 Species:  pennaceus Cloned:   Yes DNA Sequence: (SEQ IDNO:161) ATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGCACCTAGCGTTGATGCCAAAGTTCATCTGAAGACCAAAGGTGATGGGCCCCTGTCATCTTTCCGAGATAATGCAAAGAGTACCCTACAAAGACTTCAGGACAAAAGCACTTGCTGTGGCTTTAAGATGTGTATTCCTTGTCGTTAACCAGC ATGAAGGATCCTranslation: (SEQ ID NO:162)MRCLPVFVILLLLTASAPSVDAKVHLKTKGDGPLSSFRDNAKSTLQRLQD KSTCCGFKMCIPCR ToxinSequence: (SEQ ID NO:163)Ser-Thr-Cys-Cys-Gly-Phe-Lys-Met-Cys-Ile-Xaa3-Cys- Arg-{circumflex over( )} Name:     Pn9.1 Species:  pennaceus Cloned:   Yes DNA Sequence:(SEQ ID NO:164) ATGTTGCTTCTGCTGTTTGCCTTGGGCAGCTTCGTTGTGGTCCAGTCAGGACAGATAACAAGAGATGTGGACAATGGGCAGCTCGCGGACAACCGCCGTACCCTGCGATCGCAGTGGAAGCAAGTGAGTTTCTTCAAGTCACTTGATAAACGACTGACTTGTAACGATCCTTGCCAGATGCATTCCGATTGCGGCATATGTGAATGCGTGGAAAATAAATGCATATTTTTCATGTAAACGGATTGAGTTTGCTTGTCAACACAATGTCGCACTGCAGCTCTTCTCTACCGGTGGGTACATCGACCAAACGACGCATCTTTTATTTCTTTGTCTGTTTCGTTTGTTCTCCTGTGTTCATAACGTACAGAGCCCTTTAACTACCCTTACTGCTCTTCACTTAACCTGATAACCTGAAGGTCCGGTGCAGCTGGCGTAGCCTTCACAGTTTCG Translation: (SEQ IDNO:165) MLLLLFALGSFVVVQSGQITRDVDNGQLADNRRTLRSQWKQVSFFKSLDKRLTCNDPCQMHSDCGICECVENKCIFFM Toxin Sequence: (SEQ ID NO:166)Leu-Tbr-Cys-Asn-Asp-Xaa3-Cys-Gln-Met-His-Ser-Asp-Cys-Gly-Ile-Cya-Xaa1-Cys-Val-Xaa1-Asn-Lys-Cys-Ile-Phe-Phe-Met-{circumflex over ( )} Name:     Pu6.1 Species:  pulicariusCloned:   Yes DNA Sequence: (SEQ ID NO:167)ATGAAACTGACGTGTGTGGTGATCGTCGCCGTGCTGTTCCTGACGGCCTGTCAACTCAGTACAGCTGATGACTCCAGAGATGAGCAGCAGGATCCTTTGGTGAGGTCGCATCGTGAGGAGCAGAAAGCCGAGGACCCCAAGACGGCCGAGAGATGTTCAGATTTCGGATCCGACTGTGTTCCTGCTACTCATAACTGCTGCAGTGGTGAATGTTTTGGCTTCGAGGACTTCGGCTTATGCACGTAAAACTGGTCTGACGTCTGATATTCCCCCCTCTGTCCTTCATCCTCTTTTGCCTGATTCATCCATACCTATATGTGCTCCTGAACCGCTGTGTACCTTTACCCTGGTGGCTTCAGAGGACGTTATATCAAAATAAAACCGCGTTGCAATGACAAAA AAAAAAAAAAAAATranslation: (SEQ ID NO:168)MRLTCVVIVAVLFLTACQLSTADDSRDEQQDPLVRSHREEQKAEDPKTAERCSDFGSDCVPATHNCCSGECFGFEDFGLCT Toxin Sequence: (SEQ ID NO:169)Cys-Ser-Asp-Phe-Gly-Ser-Asp-Cys-Val-Xaa3-Ala-Thr-His-Asn-Cys-Cys-Ser-Gly-Xaa1-Cys-Phe-Gly-Phe-Xaa1-Asp-Phe-Gly-Leu-Cys-Thr-{circumflex over ( )} Name:     Bromosleeper-P1Species:  purpurascens Cloned:   Yes DNA Sequence: (SEQ ID NO:170)GACAGGATTGAACAAAATTCAGGATGTCAAGATTTGGAATCATGGTGCTAACCTTTCTACTTCTTGTGTCCATGGCAACCAGCCATCGTTATGCAAGAGGGAAGCAGGCGACGCGAAGGAACGCAATCAACATCAGACGGAGAAGCACACCAAAAACTGAGGCGTGCGAAGAGGTCTGTGAGCTGGAAGAAAAGCACTGCTGCTGCATAAGAAGTGACGGACCCAAATGTTCCCGTAAGTGCCTGTTGTCAATCTTCTGTTAGTTTCTGTACACTGTCTCATTCATTATCTTATCAGTACAAGTGTAAACGAGACATGTCAGAAAGTCGAAGGTTGTGCGTAATTTGATAAGTATTGTTTGCTGGGATGAACGGA Translation: (SEQ ID NO:171)MSRFGIMVLTFLLLVSMATSHRYARGKQATRRNAINTRRRSTPKTEACEEVCELEEKIICCCIRSDGPKCSRKCLLSIFC Toxin Sequence: (SEQ ID NO:172)Xaa3-Lys-Thr-Xaa1-Ala-Cys-Xaa1-Xaa1-Val-Cys-Xaa1-Leu-Xaa1-Xaa1-Lys-His-Cys-Cys-Cys-Ile-Arg-Ser-Asp-Gly-Xaa3-Lys-Cys-Ser-Arg-Lys-Cys-Leu-Leu-Ser-Ile- Phe-Cys-{circumflexover ( )} Name:     Bromosleeper-P2 Species:  purpurascens Cloned:   YesDNA Sequence: (SEQ ID NO:173)GACAGGATTGAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTAACCCTTCTACTTCTTGTGTCCATGGCAACCAACCATCAGGATAGAGGAGAGAAGCAGGTGACGCAAAGGGACGCAATCAACGTCAGACGGAGAAGATCAATCACCCAGCAAGTCGTATCTGAGGAGTGCAAAAAGTACTGTAAGAAACAGAACAAGAATTGCTGCAGCAGTAAACATGAAGAACCCAGATGTGCCAAGATATGCTTCGGATAGTTTCTGTACACGGTCTCATTCATTATTTTATCAGTACAAGTTAAACGAGACCTATCAGAAGTCGAAGGTTGTGCATAATTTGATAAACATTGTTTGCTGGGATGAACGGA Translation: (SEQ ID NO:174)MSGLGTMVLTLLLLVSMATNIIQDRGEKQVTQRDAINVRRRRSITQQVVSEECKIKYCKKQNKNCCSSKHEEPRCAIKICFG Toxin Sequence: (SEQ ID NO:175)Val-Val-Ser-Xaa1-Xaa1-Cys-Lys-Lys-Xaa5-Cys-Lys-Lys-Gln-Asn-Lys-Asn-Cys-Cys-Ser-Ser-Lys-His-Xaa1-Xaa1-Xaa3-Arg-Cys-Ala-Lys-Ile-Cys-Phe-# Name:     P29Species:  purpurascens Isolated: Yes Toxin Sequence: (SEQ ID NO:176)Asp-Cys-Cys-Gly-Val-Lys-Leu-Xaa1-Met-Cys-His-Xaa3-Cys-Leu-Cys-Asp-Asn-Ser-Cys-Lys-Asn-Xaa5-Gly-Lys-# Name:     P4.1Species:  purpurascens Cloned:   Yes DNA Sequence: (SEQ ID NO:177)ATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCTTCACTTCAGATCGTGCATCGGATGACAGGAATACCAACGACAAAGCATCTCGCCTGCTCTCTCACGTTGTCAGGGGATGCTGTGGTAGCTATCCCAATGCTGCCTGTCATCCTTGCGGTTGTAAAGATAGGCCATCGTATTGTGGTCAAGGACGCTGATGCTCCAGGACCCTCTGAACCACGACGT Translation: (SEQ ID NO:178)MFTVFLLVVLATTVVSFTSDRASDDRNTNDKASRLLSHVVRGCCGSYPNA ACHPCGCKDRPSYCGQGRToxin Sequence: (SEQ ID NO:179)Gly-Cys-Cys-Gly-Ser-Xaa5-Xaa3-Asn-Ala-Ala-Cys-His-Xaa3-Cys-Gly-Cys-Lys-Asp-Arg-Xaa3-Ser-Xaa5-Cys- Gly-Gln-# Name:     P4.2Species:  purpurascens Cloned:   Yes DNA Sequence: (SEQ ID NO:180)ATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCTTCACCGTAGATCGTGCAACTGATGGCAGGAGTGCTGCAGCCATAGCGTTTGCCCTGATCGCTCCGACCGTCCGGGAAGGATGCTGTTCTAATCCTGCCTGTCATCCTTGCGGTTGTAAAGATAGGCCATCGTATTGTGGTCAAGGACGCTGATGCTCCAGGACCCTCTGAACCACGACGT Translation: (SEQ ID NO:181)MFTVFLLVVLATTVVSFTVDRATDGRSAAAIAFALIAPTVREGCCSNPAC HPCGCKDRPSYCGQGRToxin Sequence: (SEQ ID NO:182)Xaa1-Gly-Cys-Cys-Ser-Asn-Xaa3-Ala-Cys-His-Xaa3-Cys-Gly-Cys-Lys-Asp-Arg-Xaa3-Ser-Xaa5-Cys-Gly-Gln- # Name:     P8.1Species:  purpurascens Cloned:   Yes DNA Sequence: (SEQ ID NO:183)ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCACCCTGGCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGGAAAACACGCCTGACGAGGGACTTCTATCGTACTCTGCCAGTGTCTACTAGAGGATGCAGCGGCTCCCCTTGTTTTAAAAACAAAACGTGTCGGGATGAATGCATATGCGGCGGCTTATCCAATTGTTGGTGTGGCTACGGCGGTAGTCGAGGATGCAAGTGTACATGTAGAGAGTGATTAATCGACTCTTTAACTCGTTGAATTATTTAAAAAATCCAGAGCAATATGTTCGAGAAAAACCGAAGAC Translation: (SEQ ID NO:184)MMSKMGAMFVLLLLFTLASSQQEGDVQARKTRLTRDFYRTLPVSTRGCSGSPCFKNKTCRDECICGGLSNCWCGYGGSRGCKCTCRE Toxin Sequence: (SEQ ID NO:185)Gly-Cys-Ser-Gly-Ser-Xaa3-Cys-Phe-Lys-Asn-Lys-Thr-Cys-Arg-Asp-Xaa1-Cys-Ile-Cys-Gly-Gly-Leu-Ser-Asn-Cys-Xaa4-Cys-Gly-Xaa5-Gly-Gly-Ser-Arg-Gly-Cys-Lys-Cys-Thr-Cys-Arg-Xaa1-{circumflex over ( )} Name:     U021 homologSpecies:  purpurascens Cloned:   Yes DNA Sequence: (SEQ ID NO:186)CGACCTCAAGAGGGATCGATAGCAGTTCATGATGTCTAAACTGGGAGCCTTGTTGACCATCTGTCTGCTTCTGTTTCCCATTACTGCTCTTCTGATGGATGGAGATCAACCTGCAGACCGACCTGCAGAACGTATGGATTACGACATTTCATCTGAGGTGCATCGTTTGCTTGAAAGGAGACACCCGCCCTGTTGCATGTACGGCAGATGCCGTCGATATCCCGGATGCTCTAGTGCCTCTTGTTGCCAGGGAGGATAACGTGTTGATGACCAACTTTGTTACACGGCTACGTCAAGTGTCTACTGAATAAGTAAAACGATTGCAGT Translation: (SEQ ID NO:184)MMSKLGALLTICLLLFPITALLMDGDQPADRPAERMDYDISSEVHRLLERRHPPCCMYGRCRRYPGCSSASCCQGG Toxin Sequence: (SEQ ID NO:188) His-Xaa3-Xaa3-Cys-Cys-Met-Xaa5-Gly-Arg-Cys-Arg-Arg-Xaa5-Xaa3-Gly-Cys-Ser-Ser-Ala-Ser-Cys-Cys-Gln- Gly-#Name:     ψ-PIIIF Species:  purpurascens Isolated: Yes Toxin Sequence:(SEQ ID NO:189) Gly-Xaa3-Xaa3-Cys-Cys-Leu-Xaa5-Gly-Ser-Cys-Arg-Xaa3-Phe-Xaa3-Gly-Cys-Xaa5-Asn-Ala-Leu-Cys-Cys- Arg-Lys-#Name:     Qc6.4 Species:  quercinus Cloned:   Yes DNA Sequence: (SEQ IDNO:190) GGATCCATGAAACTGACGTGCGTGGTGATCATCGCCGTGCTGTTTCTGACAGCCAGTCAGCTCGTTACAGCTGATTACACCAGAGATAAATGGCAATACCCTGCAGCGAGTTTGAGAGGCGGAATGTGGAATTTGAGAGATACCAGGGCGTGCTCGCAAGTAGGTGAAGCTTGTTTTCCTCAGAAACCTTGCTGCCCTGGATTCCTTTGCAATCACATCGGAGGCATGTGCCACCACTAGTAACAGTCTGGCATCTGATATTTCCCCTCTGCGCTCCACCCTCTTTTGGCTGATTCATCCTTACCTGTGTGTGGTCATGAACCACTCAGTAGCTACACCTCTGGTGGCTTCAGAGGACGTATATCAAAATAAAACCACATTGCAAAAAAAAAAAAAAAAA Translation: (SEQ IDNO:191) MKLTCVVIIAVLFLTASQLVTADYTRDKWQYPAASLRGGMWNLRDTRACSQVGEACFPQKPCCPGFLCNHIGGMCIIII Toxin Sequence: (SEQ ID NO:192)Ala-Cys-Ser-Gln-Val-Gly-Xaa1-Ala-Cys-Phe-Xaa3-Gln-Lys-Xaa3-Cys-Cys-Xaa3-Gly-Phe-Leu-Cys-Asn-His-Ile-Gly-Gly-Met-Cys-His-His-{circumflex over ( )} Name:     QcIISpecies:  quercinus Isolated: Yes Toxin Sequence: (SEQ ID NO:193)Asp-Cys-Gln-Xaa3-Cys-Gly-His-Asn-val-Cys-Cys-{circumflex over ( )}Name:     EST17l Species:  radiatus Cloned:   Yes DNA Sequence: (SEQ IDNO:194) CATGAACTGTCTCGTACTGGCTTTGGTTACCATCGGTCTTCTGGCTGCAACAACCGCAGCCCCTCTGGACACCACCACGGTCCTCCTCAGCACAACTACACGCGATGTCAAGGGCTGTGTGTACGAGGGCATAGAGTACAGTGTCGGAGAGACCTACCAGGCAGACTGCAACACGTGTCGCTGTGATGGCTTTGACCTGGCTACATGCACCGTCGCGGGCTGCACAGGCTTTGGACCCGAGTGATTGGTACTATTCCACACCTAGCAATGTTCACACTGGAACCGGAACTTGATACTACCTTCTAAATATAATCAATTTGTTTCAAAAGGCCCAAA Translation: (SEQ ID NO:195)MNCLVLALVTIGLLAATTAAPLDTTTVLLSTTTRDVKGCVYEGIEYSVGETYQADCNTCRCDGFDLATCTVAGCTGFGPE Toxin Sequence: (SEQ ID NO:196)Gly-Cys-Val-Xaa5-Xaa1-Gly-Ile-Xaa1-Xaa5-Ser-Val-Gly-Xaa1-Thr-Xaa5-Gln-Ala-Asp-Cys-Asn-Thr-Cys-Arg-Cys-Asp-Gly-Phe-Asp-Leu-Ala-Thr-Cys-Thr-Val-Ala-Gly-Cys-Thr-Gly-Phe-Gly-Xaa3-Xaa1-{circumflex over ( )} Name:     EST202Species:  radiatus Cloned:   Yes DNA Sequence: (SEQ ID NO:197)GTGAGAGTCCAACAGCCCAAACCTTTCAACTCACTATGTGGCAGTTGCAGTTTTCAACGTCTGGACAGGATTCAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTAACCCTTCTACTTCTTGTGTCCATGGCAACCAGTCGTCAGGATAGAGGAGTGGGACAGCTGATGCCACGCGTCTCGTTCAAAGCCTGCAAATCAAATTATGATTGCCCCCAGCGTTTCAAATGCTGCAGTTACACCTGGAATGGATCCAGTGGATACTGTAAACGTGTTTGCTATCTTTATCGTTAGTGTAATACACAAAGTGACTCTGTTCATTCCTCTCCATCATCTCTTTAGAAACAACACGGTGTCGAGATCGTTTCTTTGTGATGAAGAGTAGTATCACGGGCAGAGTTCACTAGAGATCTCAAATGAAAAACAAGATTATTTAGTAAGTTGGGGAAAATCTGGATCTCGAAAAGATTCCTTGAAAACTCCGTATTTAACACGCTTGAGAGATGATAATAAAGAATTCTGAAAGACAAA Translation: (SEQ ID NO:198)MSGLGIMVLTLLLLVSMATSRQDRGVGQLMPRVSFKACKSNYDCPQRFKC CSYTWNGSSGYCKRVCYLYRToxin Sequence: (SEQ ID NO:199)Ala-Cys-Lys-Ser-Asn-Xaa5-Asp-Cys-Xaa3-Gln-Arg-Phe-Lys-Cys-Cys-Ser-Xaa5-Thr-Xaa4-Asn-Gly-Ser-Ser-Gly-Xaa5-Cys-Lys-Arg-Val-Cys-Xaa5-Leu-Arg-{circumflex over ( )}Name:     R8.1 Species:  radiatus Cloned:   Yes DNA Sequence: (SEQ IDNO:200) ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCACCCTGGCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGGAAAACACACCCGAAGAGAGAGTTCCAACGTATTCTGCTAAGGTCTGGCAGAAAGTGCAATTTCGACAAATGTAAAGGTACCGGAGTCTACAATTGTGGGGAATCCTGCTCATGCGAAGGTTTGCACAGTTGTCGCTGCACTTATAACATCGGTTCTATGAAGTCTGGATGCGCGTGTATTTGTACATACTATTAATGATTAATTGACTCGTTTAACTCGTTGAACGATTTAAAAAATCCAGAGCAATATGTTCGAGAAAAACC GAAGAC Translation:(SEQ ID NO:201) MIMSKMGAMFVLLLLFTLASSQQEGDVQARKTHPKREFQRILLRSGRKCNEDKCKGTGVYNCGESCSCEGLHSCRCTYNIGSMKSGCACICTYY Toxin Sequence: (SEQ IDNO:202) Lys-Cys-Asn-Phe-Asp-Lys-Cys-Lys-Gly-Thr-Gly-Val-Xaa5-Asn-Cys-Gly-Xaa1-Ser-Cys-Ser-Cys-Xaa1-Gly-Leu-His-Ser-Cys-Arg-Cys-Thr-Xaa5-Asn-Ile-Gly-Ser-Met-Lys-Ser-Gly-Cys-Ala-Cys-Ile-Cys-Thr-Xaa5-Xaa5- {circumflex over ( )}Name:     R8.2 Species:  radiatus Cloned:   Yes DNA Sequence: (SEQ IDNO:203) ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCACCCTGGCATCCAGGCAGCAGGAAGGAGATGTCCAGGCAAGGAAAACACGCCTGACGAGCGACTTCTATAGTGTTCTGCAAAGGTATGGACTAGGATGCGCTGGCACTTGTGGTTCAAGCAGCAATTGTGTTAGAGATTATTGTGACTGCCCAAAACCCAATTGTTACTGCACTGGCAAAGGCTTTCGTCAACCAGGATGCGGGTGTTCATGTTTGGGGTGATTAATTGGCTCTTTTAACTCGTTGAACGATTTAAAAAATCCAGAGCAATATGTTCGAGAAAAACCGAAGAC Translation: (SEQ ID NO:204)MMSKMGAMFVLLLLFTLASRQQEGDVQARKTRLTSDFYSVLQRYGLGCAGTCGSSSNCVRDYCDCPKIPNCYCTGKGFRQPGCGCSCLG Toxin Sequence: (SEQ ID NO:205)Xaa5-Gly-Leu-Gly-Cys-Ala-Gly-Thr-Cys-Gly-Ser-Ser-Ser-Asn-Cys-Val-Arg-Asp-Xaa5-Cys-Asp-Cys-Xaa3-Lys- Xaa3 -Asn-Cys-Xaa5-Cys-Thr-Gly-Lys-Gly-Phe-Arg- Gln-Xaa3-Gly-Cys-Gly-Cys-Ser-Cys-Leu-#Name:     Bromosleeper-Sn Species:  sponsalis Cloned:   Yes DNASequence: (SEQ ID NO:206)GACAGGATTGAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTGACCCTTTTGCTTCTTGTGTCCATGGCAACCAGCCATAAGGATGGAGGAGAGAAGCAGGCGATGCAAAGGGACGCAATCAACGTCAGACTGAGAAGATCACTCACTCGGAGAGCAGTAACTGAGGCGTGCACGGAGGACTGTAAGACTCAGGACAAGAAGTGCTGCGGCGAAATGAATGGACAACACACATGTGCCAAGATATGCCTCGGATAGTCTCTGTACGCTGTCTCATTCATTATCTCATCAGTACAAGTGTAAACGAGACAGGTCAGAAAGTCGAAGGTTGTTCGAAATTTGATAAGCATTGTTTACTGGGACGAACGGA Translation: (SEQ ID NO:207)MSGLGIMVLTLLLLVSMATSHKDGGEKQAMQRDAINVRLRRSLTRRAVTEACTEDCKTQDKKCCGEMNGQHTCAKICLG Toxin Sequence: (SEQ ID NO:208)Ala-Val-Thr-Xaa1-Ala-Cys-Thr-Xaa1-Asp-Cys-Lys-Thr-Gln-Asp-Lys-Lys-Cys-Cys-Gly-Xaa1-Met-Asn-Gly-Gln-His-Thr-Cys-Ala-Lys-Ile-Cys-Leu-# Name:     Contryphan-Sm-dW4, V7Species:  stercusmuscarum Isolated: Yes Toxin Sequence: (SEQ ID NO:209)Gly-Cys-Xaa3-Xaa4-Gln-Xaa3-Val-Cys-# Name:     Conopressin-SSpecies:  striatus Isolated: Yes Toxin Sequence: (SEQ ID NO:210)Cys-Ile-Ile-Arg-Asn-Cys-Xaa3-Arg-Gly-# Name:     S6.4 Species:  striatusCloned:   Yes DNA Sequence: (SEQ ID NO:211)AGGTCGACTCGCTGCTTGCCTGACGGAACGTCTTGCCTTTTTAGTAGGATCAGATGCTGCGGTACTTGCAGTTCAATCTTAAAGTCATGTGTGAGCTGATCCAGCGGTTGATCTTCCTCCCTCTGTGCTCCATCCTTTTCTGCCTGAGTTCTCCTTACCTGAGAGTGGTCATGAACCACTCATCACCTACTCTTCTGGAGGCTTCAGAGGAGCTACAGTGAAATAAAAGCCGCATTGC Translation: (SEQ ID NO:212)STRCLPDGTSCLFSRIRCCGTCSSILKSCVS Toxin Sequence: (SEQ ID NO:213)Cys-Leu-Xaa3-Asp-Gly-Thr-Ser-Cys-Leu-Phe-Ser-Arg-Ile-Arg-Cys-Cys-Gly-Thr-Cys-Ser-Ser-Ile-Leu-Lys-Ser-Cys-Val-Ser-{circumflex over ( )} Name:     U010 homologSpecies:  striatus Cloned:   Yes DNA Sequence: (SEQ ID NO:214)CGGCTTCTAATACGACTCACTATAGGGCAAGCAGTGGTAACAACGCAGAGTACGCGGGGGGACGGCAGACCAGCTGGGGACCAGACAGACGTCAAACAGCATCGCAGTCAGGTGTGGAGATCCCAAGACACCCAGAAGAAGGAGACAGAAGAGTTATCGTTCGTAACACAATGGCCATGAACATGTCGATGACACTCTGCATGTTTGTAATGGTCGTCGTGGCAGCCACTGTCATTGATTCCACTCAGTTACAAGAACCAGATCTCAGTCGCATGCGACGCAGCGGGCCTGCTGACTGTTGCAGGATGAAAGAGTGTTGCACCGACAGAGTGAACGAGTGTCTACAGCGCTATTCTGGCCGGGAAGATAAATTCGTTTCGTTTTGTTATCAGGAGGCCACAGTCACATGTGGATCTTTTAACGAAATCGTGGGCTGTTGCTATGGATATCAAATGTGCATGATACGAGTTGTGAAACCGAACAGTCTAAGTGGGGCCCATGAGGCGTGCAAAACCGTTTCTTGTGGTAACCCTTGCGCTTGAGGTGTCCTCGCGCCACGTCACCTGTGTACAGCGCCGTCACCAGAGCCCTGATCTTTATGCCCTTATCTGTCTTTTTGCTCTTTCACTCTCTGAAGTCTTGAGGTTTGTTCCATTCTTGTCAATCATCTCACGCGCATCCAAGTAAATAAAGGTGACGT GACAAAC Translation:(SEQ ID NO:215) MAMNMSMTLCMFVMVVVAATVIDSTQLQEPDLSRMRRSGPADCCRMKECCTDRVNECLQRYSGREDKFVSFCYQEATVTCGSFNEIVGCCYGYQMCMRVYKPNSLSGAHEACKTVSCGNPCA Toxin Sequence: (SEQ ID NO:216)Ser-Gly-Xaa3-Ala-Asp-Cys-Cys-Arg-Met-Lys-Xaa1-Cys-Cys-Thr-Asp-Arg-Val-Asn-Xaa1-Cys-Leu-Gln-Arg-Xaa5-Ser-Gly-Arg-Xaa1-Asp-Lys-Phe-Val-Ser-Phe-Cys-Xaa5-Gln-Xaa1-Ala-Thr-Val-Thr-Cys-Gly-Ser-Phe-Asn-Xaa1-Ile-Val-Gly-Cys-Cys-Xaa5-Gly-Xaa5-Gln-Met-Cys-Met-Ile-Arg-Val-Val-Lys-Xaa3-Asn-Ser-Leu-Ser-Gly-Ala-His-Xaa1-Ala-Cys-Lys-Thr-Val-Ser-Cys-Gly-Asn-Xaa3- Cys-Ala-{circumflexover ( )} Name:     WG002 Species:  striatus Isolated: Yes ToxinSequence: (SEQ ID NO:217)Xaa4-Ser-Xaa4-Arg-Met-Gly-Asn-Gly-Asp-Arg-Arg-Ser- Asp-Gln-{circumflexover ( )} Name:     Sx8.1 Species:  striolatus Cloned:   Yes DNASequence: (SEQ ID NO:218)ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTGACCCTGGCATCCAGCCAGCAGGAGGGAGATGTCCAGGCAAGGAAAACAAGCCTGAAGAGCGACTTCTATCGTGCTCTGAGACCGTATGACAGACAGTGCACTTTTGTCAACAATTGTCAACAGAACGGTGCGTGTAACGGTGATTGCTCTTGCGGGGACCAGATTTGTAAATGCGGTTATAGAATCAGTCCTGGGAGGTCAGGATGCGCGTGTACTTGTAGAAATGCCAAATGAATCACTTAACTCGTTGAAAGATTTTTAAAAATCCAGAGCTATATGTTCGAGAAAAACCGAAGAC Translation: (SEQ ID NO:219)MMSKMGAMFVLLLLLTLASSQQEGDVQARKTSLKSDFYRALRIPYDRQCTFVNNCQQNGACNGDCSCGDQICKCGYRISPGRSGCACTCRNAK Toxin Sequence: (SEQ IDNO:220) Xaa2-Cys-Thr-Phe-Val-Asn-Asn-Cys-Gln-Gln-Asn-Gly-Ala-Cys-Asn-Gly-Asp-Cys-Ser-Cys-Gly-Asp-Gln-Ile-Cys-Lys-Cys-Gly-Xaa5-Arg-Ile-Ser-Xaa3-Gly-Arg-Ser-Gly-Cys-Ala-Cys-Thr-Cys-Arg-Asn-Ala-Lys-{circumflex over ( )}Name:     Ts6.3 Species:  tessulatus Cloned:   Yes DNA Sequence: (SEQ IDNO:221) GGATCCATGAAACTGACGTGTGTGGTGATCATCGCCGTGCTGTTCCTGACGGCCTGTCAATTCATTATAGCTGATTTCTCCAGAGATAAGCGGGTACATCGTGCAGAGAGGTTGAGAGACATAATGCAGAATTTCAGAGGTACCAGGTCGTGCGCGGAATTTGGTGAAGTTTGTAGTTCTACCGCTTGCTGCCCTGATTTGGATTGCGTTGAGGCCTATTCACCCATCTGTCTCTGGGAATAGTCTGGCATCTGATATTTCCCGTCTGTGCTCTACCTACTTCTGCCGGATTCATCCATACCTATGTGTGGCCATGAACCACTCAGTACCTACACCTCTGGTGGCTTCCTAGGGACGTATATCAAAATAAAACCACATTGCAAAAAAAAAAAAAAAAA Translation: (SEQ IDNO:222) MKLTCVVIIAVLFLTACQFIIADFSRDKRVHRAERLRDIMQNFRGTRSCAEFGEVCSSTACCPDLDCVEAYSPICLWE Toxin Sequence: (SEQ ID NO:223)Ser-Cys-Ala-Xaa1-Phe-Gly-Xaa1-Val-Cys-Ser-Ser-Thr-Ala-Cys-Cys-Xaa3-Asp-Leu-Asp-Cys-Val-Xaa1-Ala-Xaa5-Ser-Xaa3-Ile-Cys-Leu-Xaa4-Xaa1-{circumflex over ( )} Name:     4/43SNX Species:  textile Isolated: Yes Cloned:   Yes DNA Sequence: (SEQ IDNO:224) CGATTGCAGGGGTTACGATGCGCCGTGTAGCTCTGGCGCGCCATGTTGTGATTGGTGGACATGTTCAGCACGAACCAACCGCTGTTTTTAGGCTGACCACAAGCCATCCGACATCACCACTCTCCTCTTCAGAGGCTTCAAGGCTTTTTGTTCTCCTTTTGAAGAATCTTTACGAGTGAACAAACAAGTAGAATAGCACGTTTTTCCCCCTTTGAAAAATCAATAATGGAGGTTAAACAAAACTGTCTTCTTCAATAAAGATTTTATCATAAT Translation: (SEQ ID NO:225)IQGGGDERQKAKIMThSRSDRDCRGYDAPCSSGAPCCDWWTCSARTNRCF Toxin Sequence: (SEQID NO:226) Asp-Cys-Arg-Gly-Xaa5-Asp-Ala-Xaa3-Cys-Ser-Ser-Gly-Ala-Xaa3-Cys-CyS-Asp-Xaa4-Xaa4-Thr-Cys-Ser-Ala-Arg-Thr-Asn-Arg-Cys-Phe-{circumflex over ( )} Name:     convulsionSpecies:  textile Isolated: Yes Toxin Sequence: (SEQ ID NO:227)Asn-Cys-Xaa3-Xaa5-Cys-Val-Val-Xaa5-Cys-Cys-Xaa3-Xaa3-Ala-Xaa5-Cys-Xaa1-Ala-Set-Gly-Cys-Arg-Xaa3- Xaa3-# Name:     Tx1.6Species:  textile Cloned:   Yes DNA Sequence: (SEQ ID NO:228)ATGCACTGTCTCCCAATCTTCGTCATTCTTCTGCTGCTGACTGCATCTGGACCTAGCGTTGATGCCCAACTGAAGACCAAAGATGATGTGCCCCTGTCATCTTTCCGAGATCATGCAAAGAGTACCCTACGAAGACTTCAGGACAAACAGACTTGCTGTGGCTATAGGATGTGTGTTCCTTGTGGTTAACCAGCATGAAG GATCC Translation:(SEQ ID NO:229) MHCLPIFVILLLLTASGPSVDAQLKTKDDVPLSSFRDHAKSTLRRLQDKQTCCGYRMCVPCG Toxin Sequence: (SEQ ID NO:230)Xaa2-Thr-Cys-Cys-Gly-Xaa5-Arg-Met-Cys-Val-Xaa3- Cys-H Name:     Tx6.14Species:  textile Cloned:   Yes DNA Sequence: (SEQ ID NO:231)GTTATGGAGCGATTGCTATAGTTGGTTAGGATCATGTATTGCGCCCTCGCAGTGTTGTTCTGAGGTTTGTGATTATTACTGCCGCCTATGGCGATGAACT CGGACCACAAGCCATTranslation: (SEQ ID NO:232) LWSDCYSWLGSCIAPSQCCSEVCDYYCRLWR ToxinSequence: (SEQ ID NO:233) Asp-Cys-Xaa5-Ser-Xaa4-Leu-Gly-Ser-Cys-Ile-Ala-Xaa3-Ser-Gln-Cys-Cys-Ser-Xaa1-Val-Cys-Asp-Xaa5-Xaa5-Cys-Arg-Leu-Xaa4-Arg-{circumflex over ( )} Name:     Tx6.3Species:  textile Cloned:   Yes DNA Sequence: (SEQ ID NO:234)AGCTGACGAATGAAAAATTCCGAGAATGTCAAGCTCAGCAAGAGAAAATGTGTGGAACAATGGAAATACTGCACCCGAGAGTCCTTATGTTGCGCGGGTTTGTGTTTGTTTAGTTTCTGCATTCTATAACGCTAATCCAGAGTCGTATATTCCGTCTAAGCTCCACCTGGCACTGTCTGGTATGTTCCTGCCAGTGACTGGTCTCATACCGCTTAGACTCTGGTCCGTCTTCTCTGCAACCACAGGAGAACGTGCATTATTACAATAAACGCATACTGC Translation: (SEQ ID NO:235)RMKNSENVKLSKRKCVEQWKYCTRESLCCAGLCLFSFCIL Toxin Sequence: (SEQ ID NO:236)Lys-Cys-Val-Xaa1-Gln-Xaa4-Lys-Xaa5-Cys-Thr-Arg-Xaa1-Ser-Leu-Cys-Cys-Ala-Gly-Leu-Cys-Leu-Phe-Ser-Phe-Cys-Ile-Leu-{circumflex over ( )} Name:     Tx6.7 Species:  textileCloned:   Yes DNA Sequence: (SEQ ID NO:237)CAGAGCCGCTCTGGTGTGCAGACCTGTCTCCAGCCCTCCGTCTCCCTGATCGGTGGTTCTGCCTGCATAGCTGTCTTCTCCACGAAGCTTTCCACAGGTATAAATAACGCTTCAGTCTCCCGTCCTGTATTGGGCCGCCGTTACAAGCCAGACCGATACAGCCAGGTCCAGTCTACTTTGCGAGTGAGTTAAAAGCTCCAGCATTCTACCAGCATCACCAGAATGAAGGTGAGCAGCGTGCTGATCGTGGCTACGCTGACACTGACCGCAGGCCAGCTGGTTAGTGCTTCTTCCCATTACTCAAAAGATGTCCAGATTCTTCCTTCTGTGAGATCAGCTGACGAAGTGGAAAATTCCGAGAATGTCAGGCTCAGCAAGAGAAGATGTGTGGAACAATGGGAAGTCTGCGGCATAATCTTGTTCTCCTCATCATGTTGCGGGCAGTTGTGTTTGTTTGGTTTCTGCGTTCTATAACGCTAATCCAGAGTCGTATATTCCGT CTAAGCTCCATranslation: (SEQ ID NO:238)MKVSSVLIVATLTLTAGQLVSASSHYSKDVQILPSVRSADEVENSENVRLSKRRCVEQWEVCGIILFSSSCCGQLCLFGFCVL Toxin Sequence: (SEQ ID NO:239)Cys-Val-Xaa1-Gln-Xaa4-Xaa1-Val-Cys-Gly-Ile-Ile-Leu-Phe-Ser-Ser-Ser-Cys-Cys-Gly-Gln-Leu-Cys-Leu-Phe-Gly-Phe-Cys-Val-Leu-{circumflex over ( )} Name:     TxVIIASpecies:  textile Isolated: Yes Toxin Sequence: (SEQ ID NO:240)Cys-Gly-Gly-Xaa5-Ser-Thr-Xaa5-Cys-Xaa1-Val-Asp-Ser-Xaa1-Cys-Cys-Ser-Asp-Asn-Cys-Val-Arg-Ser-Xaa5- Cys-Thr-Leu-Phe-#Name:     U030 Species:  textile Isolated: Yes Toxin Sequence: (SEQ IDNO:241) Gly-Cys-Asn-Asn-Ser-Cys-Gln-Xaa1-His-Ser-Asp-Cys-Xaa1-Ser-His-Cys-Ile-Cys-Thr-Ser-Arg-Gly-Cys-Gly- Ala-Val-Asn-#Name:     Bromosleeper-T1 Species:  tulipa Cloned:   Yes DNA Sequence:(SEQ ID NO:242) CAGGATTGAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTAACCCTTCTACTTCTTGTGTCCATGGCAACCAGTCATCGTTATGCAAGAGAAAAGCAGGCGACGCGAAGGGACGCAGTCAACGTCAGACGGAGAAGCAGACCAAAAACAAAGGAGTGCGAAAGGTACTGTGAGCTGGAGGAAAAGCACTGCTGCTGCATAAGAAGTAACGGACCCAAATGTTCCAGAATATGCATATTCAAATTTTGGTGTTAGTTTTCTGTACACTGTCCATTCATTATCTTATCAGTACAAGTGTAAACGAGACATGTCAGAAAGTCGAAGGTTGTGCGTAATTTGATAAGCATTGTTTACTGGGACGAACGGA Translation: (SEQ ID NO:243)MSGLGIMVLTLLLLVSMATSHRYAREKQATRRDAVNVRRRSRPKTKECERYCELEEKIHCCCIIRSNGPKCSRICIFKFWC Toxin Sequence: (SEQ ID NO:244)Xaa3-Lys-Thr-Lys-Xaa1-Cys-Xaa1-Arg-Xaa5-Cys-Xaa1-Leu-Xaa1-Xaa1-Lys-His-Cys-Cys-Cys-Ile-Arg-Ser-Asn-Gly-Xaa3-Lys-Cys-Ser-Arg-Ile-Cys-Ile-Phe-Lys-Phe- Xaa4-Cys-{circumflexover ( )} Name:     Bromosleeper-T2 Species:  tulipa Cloned:   Yes DNASequence: (SEQ ID NO:245)CAGGATTGAACAAAATTCAGGATGTCAGGATTGGGAATCATGGTGCTAACCCTTCTCCTTCTTGTGCTAATGACAACCAGTCATCAGGATGCAGGAGAGAAGCAGGCGATGCAAAGGGACGCAAAGAACTTCAGTCGGAGAAGATTAGTCATTCGGAGACCAAAAACAAGGGAGTGCGAAATGCAGTGTGAGCAGGAGGAGAAACACTGCTGCCGCGTAAGAGATGGTACGGGCCAATGTGCCCCTAAGTGCTTGGGAATTAACTGGTAGTTTCTGTACACTGTCTCATTCATTATCTTATCAGTACACGTGTAACGAGACATGTCAGAAAGTCGAAGGTAGTGCGTAATTTGATAAGCATTGTTTACTGGGACGAACGGA Translation: (SEQ ID NO:246)MSGLGIMVLTLLLLVLMTTSHQDAGEKQAMQRDAKNFSRRRLVIRRPKTRECEMQCEQEEKHCCRVRDGTGQCAPKCLGINW Toxin Sequence: (SEQ ID NO:247)Xaa3-Lys-Thr-Arg-Xaa1-Cys-Xaa1-Met-Gln-Cys-Xaa1-Gln-Xaa1-Xaa1-Lys-His-Cys-Cys-Arg-Val-Arg-Asp-Gly-Gln-Cys-Ala-Xaa3-Lys-Cys-Leu-Gly-Ile-Asn-Xaa4-{circumflex over ( )}Name:     T8.1 Species:  tulipa Cloned:   Yes DNA Sequence: (SEQ IDNO:248) ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCACCCTGGCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGGAAAACACGCCTGAAGAGCGACTTCTATCGTGCTCTGCCAAGGTTTGGCCCAATATGCACTTGTTTTAAAAGCCAGAACTGTCGGGGTTCTTGTGAATGCATGTCACCTCCCGGTTGTTACTGCAGTAACAATGGCATTCGTGAACGAGGATGCTCGTGTACATGTCCAGGGACTGGTTGAATGATTTGAAAAATTCAGAGCAATATGTTGCAGAAAAACCGAAGACCGAGACTTCTCACAATAAATCCATAAAGACATTAAAA AAAAAAAAAAAAATranslation: (SEQ ID NO:249)MMSKMGAMFVLLLLFTLASSQQEGDVQARKTRLKSDFYRALPRFGPICTCFKSQNCRGSCECMSPPGCYCSNNGIRERGCSCTCPGTG Toxin Sequence: (SEQ ID NO:250)Phe-Gly-Xaa3-Ile-Cys-Thr-Cys-Phe-Lys-Ser-Gln-Asn-Cys-Arg-Gly-Ser-Cys-Xaa1-Cys-Met-Ser-Xaa3-Xaa3-Gly-Cys-Xaa5-Cys-Ser-Asn-Asn-Gly-Ile-Arg-Xaa1-Arg-Gly-Cys-Ser-Cys-Thr-Cys-Xaa3-Gly-Thr-# Name:     T8.2 Species:  tulipaCloned:   Yes DNA Sequence: (SEQ ID NO:251)ATGATGTCGAAAATGGGAGCTATGTTTGTCCTTTTGCTTCTTTTCACCCTGGCATCCAGCCAGCAGGAAGGAGATGTCCAGGCAAGGAAAACACGCCTGAAGAGCGACTTCTATCGTACTCTGGCAATATCTGACAGAGGATGCACTGGCAACTGTGATTGGACGTGTAGCGGTGATTGCAGCTGCCAGGGCACATCTGACTCGTGTCACTGCATTCCACCAAAATCAATAGGCAACAGATGCCGGTGTCAGTGTAAAAGAAAAATCGAAATTGACTGATTCTTTTAACTCGTTGAACGATTTAAAAATCAGACCAATATGTAGGCAGAAAACCGAAGACTCTGAGACTCTCGTAATAATCGTAAGCAAAAAAAAAAAAAAAA Translation: (SEQ ID NO:252)MIMSKMGAMFVLLLLFTLASSQQEGDVQARKTRLKSDFYRTLAISDRGCTGNCDWTCSGDCSCQGTSDSCHCIPPKSIGNRCRCQCKRKIEID Toxin Sequence: (SEQ IDNO:253) Gly-Cys-Thr-Gly-Asn-Cys-Asp-Xaa4-Thr-Cys-Ser-Gly-Asp-Gly-Asp-Cys-Ser-Cys-Gln-Gly-Thr-Ser-Asp-Ser-Cys-His-Cys-Ile-Xaa3-Xaa3-Lys-Ser-Ile-Gly-Asn-Arg-Cys-Arg-Cys-Gln-Cys-Lys-Arg-Ile-Xaa1-Asp-{circumflex over ( )}Name:     Vr6.1 Species:  Virgo Cloned:   Yes DNA Sequence: (SEQ IDNO:254) GGATCCATGAAACTGACGTGTGTGGTGATCATCACTGTGCTGTTCCTGACGGCCAGTCAGCTCATTACAGCTGATTACTCCAGAGATCAGCGGCAGTACCGTGCAGTGAGGTTGGGAGATGAAATGCGGAATTTCAAAGGTGCCAGGGACTGCGGGGGACAAGGTGAAGGTTGTTATACTCAACCTTGCTGCCCTGGTCTGCGGTGCCGTGGCGGCGGTACTGGAGGAGGCGTATGCCAGCTGTAGTAATAGTTTGGCATCTGATATTTCCCCTCTGTGCTCCACCCTCTTTTGCCTGATTCATCCTTACCTATGTGTGGTCATGAACCACTCAGTAGCTACACCTCTGGTGGATTCAGAGAACGTATATCAAAATAAAACCACATTGCAATAAAAAAAA AAAA Translation:(SEQ ID NO:255) MKLTCVVIITVLFLTASQLITADYSRDQRQYRAVRLGDEMRNFKGARDCGGQGEGCYTQPCCPGLRCRGGGTGGGVCQL Toxin Sequence: (SEQ ID NO:256)Asp-Cys-Gly-Gly-Gln-Gly-Xaa1-Gly-Cys-Xaa5-Thr-Gln-Xaa3-Cys-Cys-Xaa3-Gly-Leu-Arg-Cys-Arg-Gly-Gly-Gly-Thr-Gly-Gly-Gly-Val-Cys-Gln-Leu-{circumflex over ( )} Name:     R6.9Species:  radiatus Cloned:   Yes DNA Sequence: (SEQ ID NO:257)ATCATGCAGAAACTGACAATCCTGCTTCTTGTTGCTGCTATACTGATGTCGACCCAGGTCCTGATTCAAGGTGGTGGAGAAAAACGCCAAAAAGTCAACATTTTTTCAAAAAGAAAGACAGATGCTGAGACCTGGTGGGAGGGCGAATGCTCTAATTGGTTAGGAAGTTGTTCGACGCCCTCAAATTGCTGTCTCAAGAGTTGTAATGGGCACTGCACATTGTGGTGATGAACTCTGACCACAAAGCCATCCAACATCACCGCTCTCCTCTTCAGAGTCTTCAAG Translation: (SEQ ID NO:258)MQKLTILLLVAMLMSTQVLIQGGGEKRQKVMFSKRLKTDAETWWEGECSNWLGSCSTPSNCCLKSCNGHCTLW Toxin Sequence: (SEQ ID NO:259)Xaa4-Xaa4-Xaa1-Gly-Xaa1-Cys-Ser-Asn-Xaa4-Leu-Gly-Ser-Cys-Ser-Thr-Xaa3-Ser-Asn-Cys-Cys-Leu-Lys-Ser-Cys-Asn-Gly-His-Cys-Thr-Leu-Xaa4-{circumflex over ( )} Name:     R6.10Species:  radiatus Cloned:   Yes DNA Sequence: (SEQ ID NO:260)ATCATGCAGAAACTGATAATCCTGCTTCTTGTTGCTGCTGTACTGATGTCCACCCAGGCCCTGATTCAAGGTGGTGGAGGAAAACGCCAACAGGCAAAGAGCAAGTATTTTTCCGAAAGAAAGGCACCTGCTAAGCGTTGGTTTGGACACGAAGAATGCACTTATTGGTTGGGGCCTTGTGAGGTGGACGACACGTGTTGTTCTGCCAGTTGTGAGTCCAAGTTCTGCGGGTTGTGGTGATGGACACTGACCACAAGTCATCCTACATCGCCACTCTCCTGTTCAGAGTCTTCAAG Translation: (SEQ IDNO:261) MQKLIILLLVAAVLMSTQALIQGGGGKRQQAKSKYFSERKAPAKRWFGHEECTYWLGPCEVDDTCCSASCESKFCGLW Toxin Sequence: (SEQ ID NO:262)Xaa4-Phe-Gly-His-Xaa1-Xaa1-Cys-Thr-Xaa5-Xaa4-Leu-Gly-Xaa3-Cys-Xaa1-Val-Asp-Asp-Thr-Cys-Cys-Ser-Ala-Ser-Cys-Xaa1-Ser-Ala-Ser-Cys-Xaa1-Ser-Lys-Phe-Cys-Gly-Leu-Xaa4-{circumflex over ( )} Name:     Wi6.1 Species:  wittigiCloned:   Yes DNA Sequence: (SEQ ID NO:263)GGATCCATGAAACTGACGTGTGTGGTGATCATCGCCTTGCTGTTCCTGACGGCCTGTCAGCTCATTACGGCTGATTACTCCAGAGATGAGCAGTCTGGCAGTACAGTGCGGTTTCTAGACAGACCACGGCGTTTTGGTTCGTTCATACCGTGCGCCCGTTTAGGTGAACCATGTACCATATGCTGCCGTCCTTTGAGGTGCCGTGAAAGCGGAACACCCACATGTCAAGTGTGATTGTCTGGCATCTGATATTTCCCCTCTGTGCCCTACCCTCTTTTGCCTGAGTCATCCATACCTGTG CTCGAG Translation:(SEQ ID NO:264) MKLTCVVIIALLFLTACQLITADYSRDEQSGSTVRFLDRPRRFGSFIPCARLGEPCTICCRPLRCRESGTPTCQV Toxin Sequence: (SEQ ID NO:265)Phe-Gly-Ser-Phe-Ile-Xaa3-Cys-Ala-Arg-Leu-Gly-Xaa1-Xaa3-Cys-Thr-Ile-Cys-Cys-Arg-Xaa3-Leu-Arg-Cys-Arg-Xaa1-Ser-Gly-Thr-Xaa3-Thr-Cys-Gln-Val-{circumflex over ( )}Name:     Rg6.6 Species:  regius Cloned:   Yes DNA Sequence: (SEQ IDNO:266) GGATCCATGAAACTGACGTGCGTGGTGATCATGGCCTCGCTGTTCCTGGCGGCCTGTCAATTCCTTACAGCTGGAGGTGACTCAAGAAGTAAGCAGCGGTATCCTGATTGGAGGCTGGGCTACCGAAAGTCCAAGTTGATGGCTAAGAAGACGTGCCTGGAACATAACAAACTATGTTGGTATGATAGAGACTGCTGCACCATATATTGTAATGAAAACAAATGCGGCGTGAAACCTCAATGAATGTTTCACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACATCTGGCGTCTGACCATTCCCCCTCTGTGCTCTATCCTCTTGTTCCTGAGTCATCCATACCTGTGCTCGAG Translation: (SEQ ID NO:267)MKLTCVVIMASLFLAACQFLTAGGDSRSKQRYPDWRLGYRKSKLMAKKTCLEHNKLCWYDRDCCTIYCNENIKCGVKPQ Toxin Sequence: (SEQ ID NO:268)Thr-Cys-Leu-Xaa1-His-Asn-Lys-Leu-Cys-Xaa4-Xaa5-Asp-Arg-Asp-Cys-Cys-Thr-Ile-Xaa5-Cys-Asn-Xaa1-Asn-Lys-Cys-Gly-Val-Lys-Xaa3-Gln-{circumflex over ( )} Name:     R6.9Species:  radiatus Cloned:   Yes DNA Sequence: (SEQ ID NO:269)ATCATGCAGAAACTGACAATCCTGCTTCTTGTTGCTGCTATACTGATGTCGACCCAGGTCCTGATTCAAGGTGGTGGAGAAAAACGCCAAAAAGTCAACATTTTTTCAAAAAGAAAGACAGATGCTGAGACCTGGTGGGAGGGCGAATGCTCTAATTGGTTAGGAAGTTGTTCGACGCCCTCAAATTGCTGTCTCAAGAGTTGTAATGGGCACTGCACATTGTGGTGATGAACTCTGACCACAAAGCCATCCAACATCACCGCTCTCCTCTTCAGAGTCTTCAAG Translation: (SEQ ID NO:270)MQKLTILLLVAAILMSTQVLIQGGGEKRQKVNWSKRKTDAETWWEGECSNWLGSCSTPSNCCLKSCNGHCTLW Toxin Sequence: (SEQ ID NO:271)Xaa4-Xaa4-Xaa1-Gly-Xaa1-Cys-Ser-Asn-Xaa4-Leu-Gly-Ser-Cys-Ser-Thr-Xaa3-Ser-Asn-Cys-Cys-Leu-Lys-Ser-Cys-Asn-Gly-His-Cys-Thr-Leu-Xaa4-{circumflex over ( )} Name:     R6.10Species:  radiatus Isolated: Yes Cloned:   Yes DNA Sequence: (SEQ IDNO:272) ATCATGCAGAAACTGATAATCCTGCTTCTTGTTGCTGCTGTACTGATGTCCACCCAGGCCCTGATTCAAGGTGGTGGAGGAAAACGCCAACAGGCAAAGAGCAAGTATTTTTCCGAAAGAAAGGCACCTGCTAAGCGTTGGTTTGGACACGAAGAATGCACTTATTGGTTGGGGCCTTGTGAGGTGGACGACACGTGTTGTTCTGCCAGTTGTGAGTCCAAGTTCTGCGGGTTGTGGTGATGGACACTGACCACAAGTCATCCTACATCGCCACTCTCCTGTTCAGAGTCTTCAAG Translation: (SEQ IDNO:273) MQKLIILLLVAAVLMSTQALIQGGGGKRQQAKSKYFSERKAPAKRWFGHEECTYWLGPCEVDDTCCSASCESKFCGLW Toxin Sequence: (SEQ ID NO:274)Xaa4-Phe-Gly-His-Xaa1-Xaa1-Cys-Thr-Xaa5-Xaa4-Leu-Gly-Xaa3-Cys-Xaa1-Val-Asp-Asp-Thr-Cys-Cys-Ser-Ala-Ser-Cys-Xaa1-Ser-Ala-Ser-Cys-Xaa1-Ser-Lys-Phe-Cys-Gly-Leu-Xaa4-{circumflex over ( )} Name:     Sf5.1 Species:  spuriusCloned:   Yes DNA Sequence: (SEQ ID NO:275)GGAAGCTGACTACAAGCAGAATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGATTCCATCTGCACCTAGCACTGATGCCCGACCGAAGACCAAAGATGATGTGCGCCTGGCATCTTTCCACGGTAAGGCAAAGCGAACCCTACAAATACCTAGGGGGAATATCCACTGTTGCACAAAATATCAGCCGTGCTGTTCTTCACCATCATAAAGGGAAATGACTTTGATGAGACCCCTGCGAACTGTCCCTGGATGTGAAATTTGGAAACGAGACTGTTCCTTTCGCGCGTGTTCGTGGAATTTCGAATGGTCGTTAATAACACGCTGCCTCTTGCAAACTACAATCTCTCTGTCCTTTATCTGTGGACTGGATGTCAACACTG Translation: (SEQ ID NO:276)MRCLPVFVILLLLIPSAPSTDARPKTKDDVRLASFHGKAKRTLQIPRGNL HCCTKYQPCCSSPS ToxinSequence: (SEQ ID NO:277)Gly-Asn-Ile-His-Cys-Cys-Thr-Lys-Xaa5-Gln-Xaa3-Gln-Xaa3-Cys-Cys-Ser-Ser-Xaa3-Ser-{circumflex over ( )} Name:     Nb5.1Species:  riobilis Cloned:   Yes DNA Sequence: (SEQ ID NO:278)ATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGCACCAAGCGTTGATGCCCGACCGAAGACCAAAGATGATGTGCTCCGGGCATCTTTCCGCGATAATGCAAAGAGTACCCTACAAAGACTTTGGAACAAACGCATCTGCTGCCCCATAATTCTTTGGTGCTGTGGTTAACCAGCATGAAGTTC CCAGGA Translation:(SEQ ID NO:279) MRCLPVFVILLLLTASAPSVDARPKTKDDVLRASFRDNAKSTLQRLWNKRICCPIILWCCG Toxin Sequence: (SEQ ID NO:280)Ile-Cys-Cys-Xaa3-Ile-Ile-Leu-Xaa4-Cys-Cys-# Name:     Bt5.1Species:  betulinus Cloned:   Yes DNA Sequence: (SEQ ID NO:281)ATGCGCTGTCTCCCAGTCTTCATCATTCTTCTGGTGCTGATTGCATCTGCACCTACCGTTGATGCCCGACCAAAGATCGAAGATGATGAGTCCCTGGCATCTTTCCATGNTCATNAACCACCATNANNGNTNCANCTTTTGAACAAACGCAATTGCTGCCCAGACTCTCCTCCGTGCTGTCATTAACCAGCATGAAGGTT CAGGA Translation:(SEQ ID NO:282) MRCLPVFIILLVLIASAPTVDARPKIEDDESLASFH?H?PP????LLKRNCCPDSPPCCH Toxin Sequence: (SEQ ID NO:283)Asn-Cys-Cys-Xaa3-Asp-Ser-Xaa3-Xaa3-Cys-Cys-His-{circumflex over ( )}Name:     t-PVA Species:  purpurascens Isolated: Yes Cloned:   Yes DNASequence: (SEQ ID NO:284)GGAATTCCAAATGATGTAATTACTGACTACATGGTCATAGTGTATACCCATTGAAAAATTTCTATGACATTTCAGTTGTTAGATCATCCAGTTCCACAGATGGAAAGACAGAGAGATAGTAGCTTGCAAGTGGCAGCGTGTTGTTAACGACCATTCGACATTCCATGAACACGTGTGAAAGGAGCAGTCTGCTTTCCAAATCTGACATCCAGGGACAGTTTGCAGGGGTCTCATCCAAAGTCATCTTCCTTTATCCCAAAGTACAGCACCGCATCTGTTTTGGACAGCAACCGCGTTTCTTCCAAAATCTTTGTAGGGTTCCTTTTGCATTATCGTGGAGATGCCAGGGGCATATCATCTTTGGTCTTCGGATGAGCATCAACGCAAGGTGCAGATGGAATCAGCAGCAGAAGAATGACGAAGACTGGCAGACAGCGCATTCTGCTTGTAGTCAGCTTCCGAATTCCAAGCCGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCG TATGACAATTCACTGGCTranslation: (SEQ ID NO:285)MRCLPFVILLLLIPSAPCVDAHPKTKDDMPLASFHDNAKGTLQRFWKKGC CPKQMRCCTLG ToxinSequence: (SEQ ID NO:286)Gly-Cys-Cys-Xaa3-Lys-Gln-Met-Arg-Cys-Cys-Thr-Leu# Name:     Af5.2Species:  ammiralis Cloned:   Yes DNA Sequence: (SEQ ID NO:287)GGAAGCTGACTACAAGCAGAATGCACTGTCTCCCAGTCGTCGTCATTCTTCTGCTGCTGACTGCATCTGGTGGACCTAGCGTTGATGCCCGACTGAAGACCAAAGATGATGTGCCCCTGTCATCTTTCCGCGATAATACAAGAGTATCCTACAAACACTTTGGAAGCGAGGCAACTGCTGTGAATTTTGGGAGTTTTGCT GTGATTAACCAGCATGAAGGTranslation: (SEQ ID NO:288)MHCLPVVVILLLLTASGGPSVDARLKTKDDVPLSSFRDNTKSILQRLWKR GNCCEFWEFCCD ToxinSequence: (SEQ ID NO:289)Gly-Asn-Cys-Cys-Xaa1-Phe-Xaa4-Xaa1-Phe-Cys-Cys- Asp-{circumflex over( )} Name:     Da5.1 Species:  dalli Cloned:   Yes DNA Sequence: (SEQ IDNO:290) GGAAGCTGACTACAAGCAGAATGCACTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGGACCTAGCGTTGATGCCCAACCGAAGACCGAAGTTGATGTGCCCCTGTCATCTTTCCGCGATAATGCAAAGCGTGCCCTACAAAGACTTCCGCGTTGCTGTGAATATTGGAAGTTGTGCTGTGGTTAACCA GCATGAAGGTranslation: (SEQ ID NO:291)MHCLPVFVILLLLTASGPSVDAQPKTEVDVPLSSFRDNAKRALQRLPRCC EYWKLCCG ToxinSequence: (SEQ ID NO:292) Cys-Cys-Xaa1-Xaa5-Xaa4-Lys-Leu-Cys-Cys-#Name:     Om5.1 Species:  omaria Cloned:   Yes DNA Sequence: (SEQ IDNO:293) GGAAGCTGACTACAAGCAGAATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTAACTGCATCTGCACCTAGCGTTGATGCCCGACCGAAGGCCAAAGATGATGTGCCCCTGGCATCTTTCCGTGATAATGCAAAGAGTACCCTACAAAGACTTCAGGACAAAACGCGTTTGCTGTGGCTATAAGTTTTTTTGCTG TCGTTAACCAGCATGAAGGTranslation: (SEQ ID NO:294)MRCLPVFVILLLLTASAPSVDARPKAKDDVPLASFRDNAKSTLQRLQDKR VCCGYKFFCCR ToxinSequence: (SEQ ID NO:295)Val-Cys-Cys-Gly-Xaa5-Lys-Phe-Phe-Cys-Cys-Arg-{circumflex over ( )}Name:     Au5.1 Species:  aulicus Cloned:   Yes DNA Sequence: (SEQ IDNO:296) GGAAGCTGACTACAAGCAGAATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGCACCTAACGTTGATGCCCAACCGAAGACCAAAGATGATGTGCCCCTGGCATCTTTGCACGATGATGCAAAGAGTGCACTACAACATTGGAACCAACGCTGCTGCCCCATGATCTATTGGTGCTGTAGTTAA CCAGCATGAAGGTranslation: (SEQ ID NO:297)MRCLPVFVILLLLTASAPNVDAQPKTKDDVPLASLHDDAKSALQHWNQRC CPMIYWCCS ToxinSequence: (SEQ ID NO:298)Cys-Cys-Xaa3-Met-Ile-Xaa5-Xaa4-Cys-Cys-Ser-{circumflex over ( )}Name:     Au5.4 Species:  aulicus Cloned:   Yes DNA Sequence: (SEQ IDNO:299) GGAAGCTGACTACAAGCAGAATGCACTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGCACCTAACGTTGATGCCCAACCGAAGACCAAAGATGATGTGCCCCTGGCATCTTTGCACGATGATGCAAACAGTGCACTACAACATTGGAACCAACGCTGCTGCCCCGAGATCTATTGGTGCTGTAGTTAA CCAGCATGAAGGTranslation: (SEQ ID NO:300)MHCLPVFVILLLLTASAPNVDAQPKTKDDVPLASLHDDAKSALQHWNQRC CPEIYWCCS ToxinSequence: (SEQ ID NO:301)Cys-Cys-Xaa3-Xaa1-Ile-Xaa5-Xaa4-Cys-Cys-Ser-{circumflex over ( )}Name:     Af5.1 Species:  ammiralis Cloned:   Yes DNA Sequence: (SEQ IDNO:302) GGAAGCTGACTACAAGCAGAATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGATTGCATCTGCACCTAGCGTTGATGCCCAACCGAAGACCAAAGATGATGTGTCCCTGGCATCTTTGCACGATAATATAAAGAGTACTCTACAAACACTTTGGAACAAACGCTGCTGCCCCCCTGTGATTTGGTGCTGTGGT TAACCAGCATAAAGGTranslation: (SEQ ID NO:303)MRCLPVFVILLLLIASAPSVDAQPKTKDDVSLASLHDNIKSTLQTLWNKR CCPPVIWCCG ToxinSequence: (SEQ ID NO:304) Cys-Cys-Xaa3-Xaa3-Val-Ile-Xaa4-Cys-Cys-#Name:     Au5.3 Species:  aulicus Cloned:   Yes DNA Sequence: (SEQ IDNO:305) GGAAGCTGACTACAAGCAGAATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGGACCTAGCGTTGATGCCCGACCGAAGACCAAAGATGATGTGCCTCTGTCATCTTTCCGCGATAACGCAAAGAGTATCCTACAAAGACGTTGGAACAACTATTGCTGCACGAATGAGCTTTGGTGCTGTGGT TAACCAGCATGAAGGTranslation: (SEQ ID NO:306)MRCLPVFVILLLLTASGPSVDARLPKTKDDVPLSSFRDNAKSILQRRWNN YCCTNLLWCCG ToxinSequence: (SEQ ID NO:307)Xaa4-Asn-Asn-Xaa5-Cys-Cys-Thr-Asn-Xaa1-Leu-Xaa4- Cys-Cys-#Name:     Da5.2 Species:  dalli Cloned:   Yes DNA Sequence: (SEQ IDNO:308) GGAAGCTGACTACAAGCAGAATGCACTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGGACCTAGCGTTGATGCCCGACCGAAGACCGAAGATGATGTGCCCCTGTCATCTTTCCGCGATAATACAAAGAGTACCCTACAAAGACTTTTGAAGCCAGTCAACTGCTGTCCTATTGATCAATCTTGCTGT TCTTAACCAGCATGAAGGTranslation: (SEQ ID NO:309)MHCLPVFVILLLLTASGPSVDARPKTEDDVPLSSFRDNTKSTLQRLLKPV NCCPIDQSCCS ToxinSequence: (SEQ ID NO:310)Xaa3-Val-Asn-Cys-Cys-Xaa3-Ile-Asp-Gln-Ser-Cys-Cys- Ser-{circumflex over( )} Name:     Cn10.3 Species:  consors Cloned:   Yes DNA Sequence: (SEQID NO:311) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCATCCCTTCAGATCGTGCATCTGAAGGCAGGAATGCCGTAGTCCACGAGAGAGCGCCTGAGCTGGTCGTTACGGCCACCACGACTTGCTGTGGTTATGATCCGATGACAATATGCCCTCCTTGCATGTGCACTCATTCCTGTCCACCAAAAAGAAAACCAGGCCGCAGAAACGACTGATGCTCGAG Translation: (SEQ ID NO:312)MFTVFLLVVLATTVVSIPSDRASEGRNAVVHERAPELVVTATTTCCGYDPMTICPPCMCTHSCPPKRKPGRRND Toxin Sequence: (SEQ ID NO:313)Ala-Xaa3-Xaa1-Leu-Val-Val-Thr-Ala-Thr-Thr-Thr-Cys-Cys-Gly-Xaa5-Asp-Xaa3-Met-Thr-Ile-Cys-Xaa3-Xaa3-Cys-Met-Cys-Thr-His-Ser-Cys-Xaa3-Xaa3-Lys-Arg-Lys- Xaa3-#Name:     A10.2 Species:  aurisiacus Cloned:   Yes DNA Sequence: (SEQ IDNO:314) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCATCCCTTCAGATCGTGCATCTGATGGCAGGAATGCCGCAGTCAACGAGAGACAATCTTGGCTGGTCCCTTCGACAATCACGACTTGCTGTGGATATGATCCGGGGACAATGTGCCCTCCTTGCAGGTGCAATAATACCTGTAAACCAAAAAAACCAAAACCAGGAAAAGGCCGCAGAAACGACTGATGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:315)MFTVFLLVVLATTVVSIPSDRASDGRNAAVNERQSWLVPSTITTCCGYDPGTMCPPCRCNNTCKPKXPKPGKGRRND Toxin Sequence: (SEQ ID NO:316)Xaa2-Ser-Xaa4-Leu-Val-Xaa3-Ser-Thr-Thr-Cys-Cys-Gly-Xaa5-Asp-Xaa3-Gly-Thr-Met-Cys-Xaa3-Xaa3-Cys-Arg-Cys-Asn-Asn-Thr-Cys-Lys-Xaa3-Lys-Lys-Xaa3-Lys- Xaa3-Gly-Lys-#Name:     Cn10.4 Species:  consors Cloned:   Yes DNA Sequence: (SEQ IDNO:317) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCATCCCTTCAGATCGTGCATCTGATGGCAGGAATGCCGTAGTCCACGAGAGAGCGCCTGAGCTGGTCGTTACGGCCACCACGACTTGCTGTGGTTATGATCCGATGACATGGTGCCCTTCTTGCATGTGCACTTATTCCTGTCCCCACCAAAGGAAAAAACCAGGCCGCAGAAACGACTGATGCTCCAGGACCCTCT GAACCACGACCTCGAGTranslation: (SEQ ID NO:318)MFTVFLLVVLATTVVSIPSDRASDGRNAVVHERAPELVVTATTTCCGYDPMTWCPSCMCTYSCPHQRKKPGRRND Toxin Sequence: (SEQ ID NO:319)Ala-Xaa3-Xaa1-Leu-Val-Val-Thr-Ala-Thr-Thr-Thr-Cys-Cys-Gly-Xaa5-Asp-Xaa3-Met-Thr-Xaa4-Cys-Xaa3-Ser-Cys-Met-Cys-Thr-Xaa5-Ser-Cys-Xaa3-His-Gln-Arg-Lys- Lys-Xaa3-#Name:     M10.3 Species:  magus Cloned: Yes DNA Sequence: (SEQ IDNO:320) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCAGTGTCGTTTCCATCCCTTCAGATCGTGCATCTGATGGCGGGAATGCCGTAGTCCACGAGAGAGCGCCTGAGCTGGTCGTTACGGCCACCACGACTTGCTGTGGTTATGATCCGATGACAATATGCCCTCCCTGCATGTGCACTCATTCCTGTCCACCAAAAGGAAAACCAGGCCGCAGGAACGACTGATGTCCAGGACCTCTGAACC ACGACNCGAGTranslation: (SEQ ID NO:321)MFTVELLVVLATSVVSIPSDRASDGGNAVVHERAPELVVTATTTCCGYDPMTICPPCMCTHSCPPKGKIPGRRNP Toxin Sequence: (SEQ ID NO:322)Ala-Xaa3-Xaa1-Leu-Val-Val-Thr-Ala-Thr-Thr-Thr-Cys-Cys-Gly-Xaa5-Asp-Xaa3-Met-Thr-Ile-Cys-Xaa3-Xaa3-Cys-Met-Cys-Thr-His-Ser-Cys-Xaa3-Xaa3-Lys-Gly-Lys- Xaa3-#Name:     A10.3 Species:  aurisiacus Cloned:   Yes DNA Sequence: (SEQ IDNO:323) GAATTCGCCCTTGAGGATCCGTGTGGTTCTGGGTCCAGAACCTGATGGCAGGAATGCCGCAGTCAACGAGAGACAGAAATGGCTGGTCCATTCGAANATCACGTATTGCTGTGGTTATAATAAGATGGACATGTGCCCTCCTTGCATGTGCACTTATTCCTGTCCCCCCCTAAAAAAAAAAAGACCAGGCCGCAGAAACGACTGATGCTCCAGGACCCTCTGAACCACGACCTCGAGCGAAGGGCGAATT C Translation: (SEQID NO:324) VVLGPEPDGRNAAVNIERQKWLVHSKITYCCGYNKMDMCPPCMCTYSCPPLKKKRPGRRNP Toxin Sequence: (SEQ ID NO:325)Xaa2-Lys-Xaa4-Leu-Val-His-Ser-Lys-Ile-Thr-Xaa5-Cys-Cys-Gly-Xaa5-Asn-Lys-Met-Asp-Met-Cys-Xaa3-Xaa3-Cys-Met-Cys-Thr-Xaa5-Ser-Cys-Xaa3-Xaa3-Leu- Lys-Lys-Lys-Arg-Xaa3-#Name:     A10.4 Species:  aurisiacus Cloned:   Yes DNA Sequence: (SEQ IDNO:326) GAATTCGCCCTTGAGGATCCGTGTGGTTCTGGGTCCAGCATTTGATGGCAGGAATGCCGCAGTCAACGAGAGAGCGCCTTGGACGGTCGTTACGGCCACCACGAATTGCTGCGGTATTACCGGGCCAGGCTGCCTTCCTTGCCGTTGTACTCAAACATGTGGCTGATGCTCCAGGACCCTCTGAACCACGACCTCGAGCG AAGGGCGAATTCTranslation: (SEQ ID NO:327)VVLGPAFDGRNAAVNERAPWTVVTATTNCCGITGPGCLPCRCTQTCG Toxin Sequence: (SEQ IDNO:328) Ala-Xaa3-Xaa4-Thr-Val-Val-Thr-Ala-Thr-Thr-Asn-Cys-Cys-Gly-Ile-Thr-Gly-Xaa3-Gly-Cys-Leu-Xaa3-Cys-Arg- Cys-Thr-Gln-Thr-Cys-#Name:     Mr1.3 Species:  marmoreus Cloned:   Yes DNA Sequence: (SEQ IDNO:329) GGAAGCTGACTACAAGCAGAATGCGCTGTCTCCCAGTCTTGATCATTCTTCTGCTGCTGACTGCATCTGCACCTGGCGTTGTTGTCCTACCGAAGACCGAAGATGATGTGCCCATGTCATCTGTCTACGGTAATGGAAAGAGTATCCTACGAGGGATTCTGAGGAACGGTGTTTGCTGTGGCTATAAGTTGTGCCTTCCA TGTTAACCAGCATGAAGGTranslation: (SEQ ID NO:330)MRCLPVLIILLLLTASAPGVVVLPKTEDDVPMSSVYGNGKSILRGILRNG VCCGYKLCLPC ToxinSequence: (SEQ ID NO:331)Asn-Gly-Val-Cys-Cys-Gly-Xaa5-Lys-Leu-Cys-Leu-Xaa3- Cys-{circumflex over( )} Name:     Pn1.5 Species:  pennaceus Cloned:   Yes DNA Sequence:(SEQ ID NO:332) GGAATTCGGAAGCTGACTACAAGCAGAATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGCACCTAGCGTTGATGCCAAAGTTCATCTGAAGACCAAAGGTGATGGGCCCCTGTCATCTTTCCGAGATAATGCAAAGAGTACCCTACAAAGACTTCAGGACAAAAGCACTTGCTGTGGCTTTAAGATGTGTATCCCTTGTAGTTAACCAGCATGAAGGATCC Translation: (SEQ ID NO:333)MRCLPYFVILLLLTASAPSVDAKVHLKTKGDGPLSSFRDNAKSTLQRLQD KSTCCGFKMCIPCS ToxinSequence: (SEQ ID NO:334)Ser-Thr-Cys-Cys-Gly-Phe-Lys-Met-Cys-Ile-Xaa3-Cys- Ser-{circumflex over( )} Name:     Pn1.6 Species:  pennaceus Cloned:   Yes DNA Sequence:(SEQ ID NO:335) GAATTCGGAAGCTGACTACAAGCAGAATGCGTTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGGACCTAGCGTTGATGCCCGACTGAAGACCAAAGATGATGTGCCCCTGTCATCTTTCCGAGATAATGCAAAGAGTACCCTACAAAGACTTCAGGACAAACGCCTTTGCTGTGGCTTTTGGATGTGTATTCCTTGTAATTAACCAGCATGAAGGATCC Translation: (SEQ ID NO:336)MRCLPVFVILLLLTASGPSVDARLKTKDDVPLSSFRDNAKSTLQRLQDKR LCCGFWMCIPCN ToxinSequence: (SEQ ID NO:337)Leu-Cys-Cys-Gly-Phe-Xaa4-Met-Cys-Ile-Xaa3-Cys-Asn- {circumflex over ( )}Name:     Pn1.7 Species:  pennaceus Cloned:   Yes DNA Sequence: (SEQ IDNO:338) GAATTCTCCCTTGGAATTCTGAAGCTGACTACAANCAGAATGCGTTGTCTCCCACTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGGACCTACTGTTGATGCCCGACTGAAGACCAAAGATGATGTGCCCCTGTCATCTTTCCGAGATAATGCAAAGAGTACCCTACAAAGACTTCAGGACAAAAGCACTTGCTGTGGCTTTAAGATGTGTATTCCTTGTGGTTAACCAGCATGAAGGATCC Translation: (SEQ IDNO:339) MRCLPLFVILLLLTASGPTVDARLKTKDDVPLSSFRDNAKSTLQRLQDKS TCCGFKMCIPCGToxin Sequence: (SEQ ID NO:340)Ser-Thr-Cys-Cys-Gly-Phe-Lys-Met-Cys-Ile-Xaa3-Cys-# Name:     Ep1.5Species:  episcopatus Cloned:   Yes DNA Sequence: (SEQ ID NO:341)GAATTCGCCCTTGGAATTCGGAAGCTGACTACAAGCAGAATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGGACCTANTGTTGATGCCAAAGTTCATCTGAAGACCAAAGGTGATGGGCCCCTGTCATCTTTCCGAGATAATGCAAAGAGTACCCTACAAAGACTTCAGGACAAAAGCACTTGCTGTGGCTATAGGATGTGTGTTCCTTGTGGTTAACCAGCATGAAGGATCC V Translation: (SEQID NO:342) MRCLPVFVILLLLTASGPSVDAKVHLKTKGDGPLSSFRDNAKSTLQRLQDKSTCCGYRMCVPCG Toxin Sequence: (SEQ ID NO:343)Ser-Thr-Cys-Cys-G1y-Xaa5-Arg-Met-Cys-Val-Xaa3-Cys- # Name:     Mr1.1Species:  marmoreus Isolated: Yes Cloned:   Yes DNA Sequence: (SEQ IDNO:344) GGCGAATACACCTGGCAGGTACTCAACGAACTTCAGGACACATTCTTTTCACCTGGACACTGGAAACTGACAACAGGCAGAATGCGCTGTCTCCCAGTCTTGATCATTCTTCTGCTGCTGACTGCATCTGCACCTGGCGTTGTTGTCCTACCGAAGACCGAAGATGATGTGCCCATGTCATCTGTCTACGGTAATGGAAAGAGTATCCTACGAGGAATTCTGAGGAACGGTGTTTGCTGTGGCTATAAGTTGTGCCATCCATGTTAACCAGCATGAAGGGAAATGACTTTGGATGAGACCCCTGCGAACTGTCCCTGGATGTGAAATTTGGAAAGCAGACTGTTCCTTTCGCACGTATTCGTGGAATTTCGAATGGTCGTAAACAACACGCTGCCACTTGCAGGCTACTATCTCTCTGTCCTTTCATCTGTGGAAATGGATGATCTAACAACTGAAATATCAGAAATTTTTCAATGGCTATACACTATGACCATGTAGTCAGTAATTATATCATTTGGACCTTTTGAAATATTTTTCAATATGTAAAGTTTTTGCACCCTGGAAAGGTCTTTTGGAGTTAAATATTTTAGTATGTTATGTTTTGCATACAAGTTATAGAATGCTGTCTTTCTTTTTGTTCCCACATCAATGGTGGGGGCAGAAATTATTTGTTTTGGTCAATGTAATTATGACCTGCATTTAGTGCTATAGTGATTGCATTTTCAGCGTGGAATGTTTAATCTGCAAACAGAAAGTGGTTGATCGACTAATAAAGATTTGCATGGCACAAAAAAAAAAAAAAAAAGTACTCTGCGTTGTTACTCGAG Translation: (SEQ ID NO:345)MRCLPVLIILLLLTASAPGVVVLPKTEDDVPMSSVYGNGKSILRGILRNG VCCGYKLCHPC ToxinSequence: (SEQ ID NO:346)Asn-Gly-Val-Cys-Cys-Gly-Xaa5-Lys-Leu-Cys-His-Xaa3- Cys-{circumflex over( )} Name:     Mr1.2 Species:  marmoreus Isolated: Yes Toxin Sequence:(SEQ ID NO:347) Gly-Val-Cys-Cys-Gly-Xaa5-Lys-Leu-Cys-His-Xaa3-Cys-{circumflex over ( )} Name:     Bn1.5 Species:  bandanus Cloned:   YesDNA Sequence: (SEQ ID NO:348)ATGCGCTGTCTCCCAGTCTTGATCATTCTTCTGCTGCTGACTGCATCTGCACCTGGCGTTGATGTCCTACCGAAGACCGAAGATGATGTGCCCCTGTCATCTGTCTACGATAATACAAAGAGTATCCTACGAGGACTTCTGGACAAACGTGCTTGCTGTGGCTACAAGCTTTGCTCACCATGTTAACCAGCATGAAGGAT CC Translation: (SEQID NO:349) MRCLPVLIILLLLTASAPGVDVLPKTEDDVPLSSVYDNTKSILRGLLDKRACCGYKLCSPC Toxin Sequence: (SEQ ID NO:350)Ala-Cys-Cys-Gly-Xaa5-Lys-Leu-Cys-Ser-Xaa3-Cys-{circumflex over ( )}Name:     Au1.4 Species:  aulicus Cloned:   Yes DNA Sequence: (SEQ IDNO:351) GGAAGCTGACTACAAGCAGAATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGGACCTAGCGTTGATGCCCGACTGAAGACCAAAGATGATGTGCCCCTGTCATCTTTCCGAGATAATGCAAAGAGTACCCTACAAAGACATCAGGACAAAAGCGTTTGCTGTGGCTATAAGCTGTGTTTTCCT TGTGGTTAACCAGCATGAAGGTranslation: (SEQ ID NO:352)MRCLPVFVILLLLTASGPSVDARLKTKDDVPLSSFRDNAKSTLQRHQDKS VCCGYKLCFPCG ToxinSequence: (SEQ ID NO:353)Ser-Val-Cys-Cys-Gly-Xaa5-Lys-Leu-Cys-Phe-Xaa3-Cys- # Name:     Tx1.7Species:  textile Cloned:   Yes DNA Sequence: (SEQ ID NO:354)CAGGATCCAATGGGGTTTGTTGTGGCTATAGGATGTGTGTTCCTTGTGGTTAACCAGCATGAAGGGAAATGACTTTGGATGAGACCCCTGCGAACTGTCCCTGGATGTGAGATTTGGAAAGCAGACTGTTCATTTTGCACGTGTTCGTGGAATTTCGAATGGTCGTTAACAACACGCTGCCACTTGCAAGCTACTATCTCTCTGTCCTTTTATCTGTGGAACTGTATGATCTAACAACTGAAATATCATANANATTTTTCAATGGGTATNCACTATGCATATGATCATGTAGGGTTCAAG GGGTCAAGATNCTranslation: (SEQ ID NO:355) GSNGVCCGYRMCVPCG Toxin Sequence: (SEQ IDNO:356) Asn-Gly-Val-Cys-Cys-Gly-Xaa5-Arg-Met-Cys-Val-Xaa3- Cys-#Name:     Tx1.6 Species:  textile Cloned:   Yes DNA Sequence: (SEQ IDNO:357) ATGCACTGTCTCCCAATCTTCGTCATTCTTCTGCTGCTGACTGCATCTGGACCTAGCGTTGATGCCCAACTGAAGACCAAAGATGATGTGCCCCTGTCATCTTTCCGAGATCATGCAAAGAGTACCCTACGAAGACTTCAGGACAAACAGACTTGCTGTGGCTATAGGATGTGTGTTCCTTGTGGTTAACCAGCATGAAG GATCC Translation:(SEQ ID NO:358) MHCLPIFVILLLLTASGPSVDAQLKTKDDVPLSSFRDHAKSTLRRLQDKQTCCGYRMCVPCG Toxin Sequence: (SEQ ID NO:359)Xaa2-Thr-Cys-Cys-Gly-Xaa5-Arg-Met-Cys-Val-Xaa3- Cys-# Name:     Af1.3Species:  ammiralis Cloned:   Yes DNA Sequence: (SEQ ID NO:360)AGAAGCTGACTACAAGCAGAATGCACTACCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGGACCTAGCGTTGATGCCCAACTGAAGACCAAAGATGATGTGCCCCTGTCATCTTTCCGAGATAATGCAAAGAGTACCCTACGAAGACTCCAGTACAAACAGGCTTGCTGTGGCTTTAAGATGTGTGTTCCT TGTGGTTAACCAGCATGAAGGTranslation: (SEQ ID NO:361)MHYLPVFVILLLLTASGPSVDAQLKTKDDVPLSSFRDNAKSTLRRLQYKQ ACCGFKMCVPCG ToxinSequence: (SEQ ID NO:362)Xaa2-Ala-Cys-Cys-Gly-Phe-Lys-Met-Cys-Val-Xaa3-Cys# Name:     Pn1.3Species:  pennaceus Cloned:   Yes DNA Sequence: (SEQ ID NO:363)ATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGCACCTAGCGTTGATGCCAAAGTTCATCTGAAGACCAAAGGTGATGGGCCCCTGTCATCTTTCCGAGATAATGCAAAGAGTACCCTACAAAGACTTCAGGACAAAAGCACTTGCTGTGGCTTTAAGATGTGTATTCCTTGTCGTTAACCAGC ATGAAGGATCCTranslation: (SEQ ID NO:364)MRCLPVFVILLLLTASALPSVDAKVHLKTKGDGPLSSFRDNAKSTLQRLQ DKSTCCGFKMCIPCR ToxinSequence: (SEQ ID NO:365)Ser-Thr-Cys-Cys-Gly-Phe-Lys-Met-Cys-Ile-Xaa3-Cys- Arg-{circumflex over( )} Name:     Pn1.4 Species:  pennaceus Cloned:   Yes DNA Sequence:(SEQ ID NO:366) CAGGATCCAATGGGGTTTGTTGTGGCTTTTGGATGTGTATTCCTTGTAATTAACCAGCATGAAGGGAAATGACTTTGGATAAGACCCCTGCGAACTGTCCTTGGATGTGAGATTTGGAAAGCAGACTGTTCCTTTTGCACGTGTTCGTGGAATTTCGAATGGTCGTTAACAACACGCTGCCACTTGCAAGCTACTATCTCTCTGTCCTTTCATCTGTGGAACTGTATGATCTAACAACTGAAATATCATAGAAATTTTTCAATGGGTATACACTATGCATATGACCATGTANGGGTCAAC AGNC Translation:(SEQ ID NO:367) GSNGVCCGFWMCIPCN Toxin Sequence: (SEQ ID NO:368)Asn-Gly-Val-Cys-Cys-Gly-Phe-Xaa4-Met-Cys-Ile-Xaa3- Cys-Asn-{circumflexover ( )} Name:     Om1.7 Species:  omaria Cloned:   Yes DNA Sequence:(SEQ ID NO:369) GGAAGCTGACTACAAGCAGAATGCGCTGTCTCCCAGTCTTCGTCATTCTTCTGCTGCTGACTGCATCTGCACCTAGCGTTGATGCCCGACCGAAGGCCAAAGATGATGTGCCCCTGTCATCTTTCCGTGATAATGCAAAGAGTACCCTACAAAGACTTCAGGACAAAGACGTTTGCTGTTACGTTAGAATGTGTCCTTGT CGTTAACCAGCATGAAGGTranslation: (SEQ ID NO:370)MRCLPVFVILLLLTASAPSVDARPKADDVPLSSFRDNAKSTLQRLQDKDV CCYVRMCPCR ToxinSequence: (SEQ ID NO:371)Asp-Val-Cys-Cys-Xaa5-Val-Arg-Met-Cys-Xaa3-Cys-Arg- {circumflex over ( )}Name:     Conophysin-R Species:  radiatus Isolated: Yes Toxin Sequence:(SEQ ID NO:372) His-Xaa3-Thr-Lys-Xaa3-Cys-Met-Xaa5-Cys-Ser-Phe-Gly-Gln-Cys-Val-Gly-Xaa3-His-Ile-Cys-Cys-Gly-Xaa3-Thr-Gly-Cys-Xaa1-Met-Gly-Thr-Ala-Xaa1-Ala-Asn-Met-Cys-Ser-Xaa1-Xaa1-Asp-Xaa1-Asp-Xaa3-Ile-Xaa3-Cys-Gln-Val-Phe-Gly-Ser-Asp-Cys-Ala-Leu-Asn-Asn-Xaa3-Asp-Asn-Ile-His-Gly-His-Cys-Val-Ala-Asp-Gly-Ile-Cys-Cys-Val-Asp-Asp-Thr-Cys-Thr-Thr-His-Leu-Gly- Cys-Leu-{circumflexover ( )} Name:     Ts10.1 Species:  tessulatus Cloned:   Yes DNASequence: (SEQ ID NO:373)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTTGTTTCCTTCAGTGCAGATCGTGCCAACGTCAAAGCGTCTGACCTGATCGCCCAGGCCACCAGAGACGGCTGTCCACCACATCCCGTTCCTGGCATGCATAAGTGCATGTGTACTAATACATGTGGTTGAAGACGCTGATGCTCCAGGACCCT CTGAACCACGACCTCGAGTranslation: (SEQ ID NO:374)MFTVFLLVVLATTVVSFSADRANVKASDLIAQATRDGCPPHPVPGMHKCM CTNTCG ToxinSequence: (SEQ ID NO:375)Asp-Gly-Cys-Xaa3-Xaa3-His-Xaa3-Val-Xaa3-Gly-Met-His-Lys-Cys-Met-Cys-Thr-Asn-Thr-Cys-# Name:     G1.4Species:  geographus Cloned:   Yes DNA Sequence: (SEQ ID NO:376)ANNTAGANTNYGTCGTANTANNGGATCNAATANTGNNTCGANATGATNANGAGTGATAAATGANNGGTGCACTNNTANTTANGNTNNTANGATNNNNATATTATTNNTANNNNNTAANANATATNGGTNNGGANNAAGAAGANTAAAAGTANNGNTTNGTGAAANAANGANNNNATGTTNNANNTCATAACNNNAATGTAAATAATANACGNNCCAGTGTGAAANNTNTCNNNNATAAAAATTCTNTNTNTNANGTNNNTGTNTGNGTGTGTGTGTGTGTGTGTGTGTGTGNGTGTGTGNGTGTGTGTGTGTGTGTGTGTGTGTGTGNGTGTGTGTNTGTGNGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTNTGTGGTTCTGGGTCCAGCATCTGATGNCAGGGATGACACAGCCAAAGACGAAGGGTCTNACATGGACAAATTGGTCGAGAAAAAAGAATGTTGCCATCCTGCCTGTGGCAAACACTACAGTTGTGGACGCTGATGCTCCAGGGTNTGAAGGANCAA Translation: (SEQ ID NO:377)SDXRDDTAKDEGSXIVIDKLVEKKECCHPACGKHYSCGR Toxin Sequence: (SEQ ID NO:378)Xaa1-Cys-Cys-His-Xaa3-Ala-Cys-GLy-Lys-His-Xaa5- Ser-Cys-# Name:     G1.5Species:  geographus Cloned:   Yes DNA Sequence: (SEQ ID NO:379)GGATCCATGTTCACCGTGTTTCTGTTGGTGGTCTTGGCAACCACTGTCGTTTCCTTCCCTTCAGAACGTGCATCTGATGGCAGGGATGACACAGCCAAAGACGAAGGGTCTGACATGGAGAAATTGGTCGAGAAAAAAGAATGTTGCAATCCTGCCTGTGGCAGACACTTCAGTTGTGGACGCTGATGCTCCAGGACCCT CTGAACCACGACTCGAGTranslation: (SEQ ID NO:380)MFTVFLLVVLATTVVSFPSERASDGRDDTAKDEGSDMEKLVEKKCCNPAC GRHFSCGR ToxinSequence: (SEQ ID NO:381)Xaa1-Cys-Cys-Asn-Xaa3-Ala-Cys-Gly-Arg-His-Phe-Ser- cys# Name:     S1.8Species:  striatus Cloned:   Yes DNA Sequence: (SEQ ID NO:382)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCTTCACTTCAGATCGTGCATCTGATGGCAGGGATGACGAAGCCAAAGACGACGGTCTGACATGCACGAATCGGACCGGAAAGGACGCGCATACTGTTGCCATCCTGCCTGTGGCCCAAACTATAGTTGTGGCACCTCATGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:383)MFTVFLLVVLATTVVSFTSDRASDGRDDEAIUZERSDMHESDRKGRAYCC HPACGPNYSCGTSCSRTLToxin Sequence: (SEQ ID NO:384)Ala-Xaa5-Cys-Cys-His-Xaa3-Ala-Cys-Gly-Xaa3-Asn-Xaa5-Ser-Cys-Gly-Thr-Ser-Cys-Ser-Arg-Thr-Leu-{circumflex over ( )}Name:     S1.9 Species:  striatus Cloned:   Yes DNA Sequence: (SEQ IDNO:385) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCTTCACTTCAGATCGTGCATCTGATGGCAGGGATGACGAAGCCAAAGACGAAAGGTCTGACATGCACGAATCGGACCGGAAAGGACGCGCATACTGTTGCCATCCTGTCTGTGGCAAAAACTTTGATTGTGGACGCTGATGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:386)MFTVFLLVVLATTVVSFTSDRASDGRDDEA1CDERSDMHESDRKGRAYCC HPVCGKNFDCGR ToxinSequence: (SEQ ID NO:387)Ala-Xaa5-Cys-Cys-His-Xaa3-Val-Cys-Gly-Lys-Asn-Phe- Asp-Cys-#Name:     Ra1.1 Species:  rattus Cloned:   Yes DNA Sequence: (SEQ IDNO:388) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCTTCCCTTCAGATCGTGCATCTGATGGCAGGGATGACGAAGCCAAGACGAAAGGTCTGACATGCACGAATCGGACCGGAATGGACGCGGATGCTGTTGCAATCCTGCCTGTGGCCCAAACTATGGTTGTGGCACCTCATGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:389)MFTVFLLVVLATTVVSFPSDRASDGRDDEAKDERSDMHESDRNGRGCCCN PACGPNYGCGTSCSRTLToxin Sequence: (SEQ ID NO:390)Gly-Cys-Cys-Cys-Asn-Xaa3-Ala-Cys-Gly-Xaa3-Asn-Xaa5-Gly-Cys-Gly-Thr-Ser-Cys-Ser-Arg-Thr-Leu-{circumflex over ( )}Name:     Ar1.1 Species:  arenatus Cloned:   Yes DNA Sequence: (SEQ IDNO:391) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTGGATTCCTTCACTCCAGTTCGTACTTCTGTTGGCAGGAGTGCTGCAGCCAACGCGTTTGACCGGATCGCTCTGACCGCCAGGCAAGATTATTGCTGTACCATTCCCAGCTGTTGGGATcGCTATAAAGAGAGATGTAGACACATACGCTGATGCTCCAGGACCCTCTGAACCACGACCTTGAG Translation: (SEQ ID NO:392)MFTVFLLVVLATTVDSFTPVRTSVGRSAAANAFDRIALTARQDYCCTTPS CWDRYKERCRHIR ToxinSequence: (SEQ ID NO:393)Xaa2-Asp-Xaa5-Cys-Cys-Tbr-Ile-Xaa3-Ser-Cys-Xaa4-Asp-Arg-Xaa5-Lys-Xaa1-Arg-Cys-Arg-His-Ile-Arg-{circumflex over ( )}Name:     Er1.1 Species:  ebumeus Cloned:   Yes DNA Sequence: (SEQ IDNO:394) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTGGATTCCTTCACTTCAGTTCGTACTTCCGTTGGCAGGAGTGCTGCAGCCAACGCGTTTGACCGGATCGCTCTGACCGCCAGGCAAGATTATTGCTGTACCATTCCCAGCTGTTGGGATCGCTATAAAGAGAGATGTAGACACATACGCTGATGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:395)MFTVFLLVVLATTVDSFTSVRTSVGRSAAANAFDRIALTARQDYCCTIPS CWDRYKERCRHIR ToxinSequence: (SEQ ID NO:396)Xaa2-Asp-Xaa5-Cys-Cys-Thr-Ile-Xaa3-Ser-Cys-Xaa4-Asp-Arg-Xaa5-Lys-Xaa1-Arg-Cys-Arg-His-Ile-Arg-{circumflex over ( )}Name:     Mil.2 Species:  miles Cloned:   Yes DNA Sequence: (SEQ IDNO:397) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACTGCTGTTCTTCCAGTCACTTTAGATCGTGCATCTGATGGAAGGAATGCAGCAGCCAACGCCAAAACGCCTCGCCTGATCGCGCCATTCATCAGGGATTATTGCTGTCATAGAGGTCCCTGTATGGTATGGTGTGGTTGAAGCCGCTGCTGCTCCAGGAC CCTCTGAACCACGACCTCGAGTranslation: (SEQ ID NO:398)MFTVFLLVVLATAVLPVTLDRASDGRNAAANAKTPRLIALPFIRDYCCHR GPCMVWCG ToxinSequence: (SEQ ID NO:399) Asp-Xaa5-Cys-Cys-His-Arg-Gly-Xaa3-Cys-Met-Val-Xaa4-Cys-# Name:     Jp1.1 Species:  jaspedius Cloned:   Yes DNASequence: (SEQ ID NO:400)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCAACTCTTCAGATCGTGGTCCAGCATCTAATAAAAGGAAGAATGCCGCAATGCTTGACATGATCGCTCAACACGCCATAAGGGGTTGCTGTTCCGATCCTCGCTGTAGATATAGATGTCGTTGAAGACGCTGCTGCTCCAGGACCCT CTGAACCACGACCTCGAGTranslation: (SEQ ID NO:401)METVELLVVLATTVVSNSSDRGPASNKRKNAAMLDMIAQHAIRGCCSDPR CRYRCR ToxinSequence: (SEQ ID NO:402)Gly-Cys-Cys-Ser-Asp-Xaa3-Arg-Cys-Arg-Xaa5-Arg-Cys- Arg-{circumflex over( )} Name:     a-OmIA Species:  omaria Isolated: Yes Toxin Sequence:(SEQ ID NO:403) Gly-Cys-Cys-Ser-His-Xaa3-Ala-Cys-Asn-Val-Asn-Asn-Xaa3-His-Ile-Cys-Gly-# Name:     a-OmIA [COOH] Species:  omariaCloned:   No Toxin Sequence: (SEQ ID NO:404)Gly-Cys-Cys-Ser-His-Xaa3-Ala-Cys-Asn-Val-Asn-Asn-Xaa3-His-Ile-Cys-Gly-{circumflex over ( )} Name:     Qc1.1Species:  quercinus Cloned:   Yes DNA Sequence: (SEQ ID NO:405)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCACTTCAGATCGTGTATCTAATGGCAGGAAAGCTGCAGCCAAATTCAAAGCGCCTGCCCTGATGGAGCTGTCCGTCAGGCAAGGATGCTGTTCAGATCCTGCCTGTGCCGTGAGCAATCCAGACATCTGTGGCGGAGGACGCTGATGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:406)MFTVFLLVVLATTVTSDRVSNGRKAAAIKFKAPALMELSVRQGCCSDPAC AVSNPDICGGGR ToxinSequence: (SEQ ID NO:407)Xaa2-Gly-Cys-Cys-Ser-Asp-Xaa3-Ala-Cys-Ala-Val-Ser-Asn-Xaa3-Asp-Ile-Cys-Gly-Gly-# Name:     Bn1.6 Species:  bandanusCloned:   Yes DNA Sequence: (SEQ ID NO:408)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTTGTTTCCTTCACTTCAAATCGTGCATTTCGTCGTAGGAATGCCGTAGCCAAAGCGTCTGACCTGATCGCTCTGAACGCCAGGAGACCAGAATGCTGTACTCATCCTGCCTGTCACGTGAGTCATCCAGAACTCTGTGGTTGAAGACGCTGACGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:409)MFTVFLLVVLATTVVSFTSNRAFRRRNAVAKASDLIALNARRPECCTHPA CHVSHPELCG ToxinSequence: (SEQ ID NO:410)Xaa3-Xaa1-Cys-Cys-Thr-His-Xaa3-Ala-Cys-His-Val-Ser-His-Xaa3-Xaa1-Leu-Cys-# Name:     Mr1.5 Species:  marmoreusCloned:   Yes DNA Sequence: (SEQ ID NO:411)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTTGTTTCCTTCACTTCAAATCGTGTTCTGGATCCAGCATTTCGTCGTAGGAATGCCGCAGCCAAAGCGTCTGACCTGATCGCTCTGAACGCCAGGAGACCAGAATGCTGTACTCATCCTGCCTGTCACGTGAGTAATCCAGAACTCTGTGGCTGAAGACGCTGATGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:412)MFTVFLLVVLATTVVSFTSNRVLDPAFRRRNAAAKASDLIAINAIUIIEC CTHPACHVSNPELCG ToxinSequence: (SEQ ID NO:413)Xaa3-Xaa1-Cys-Cys-Thr-His-Xaa3-Ala-Cys-His-Val-Ser-Asn-Xaa3-Xaa1-Leu-Cys-# Name:     Mi1.1 Species:  milesCloned:   Yes DNA Sequence: (SEQ ID NO:414)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCGTCACTTCATATCGTGCATCTCATGGCAGGAAGGACGCAGCCGACCTGAGCGCTCTGAACGACAACAATAATTGCTGTAACCATCCTGCCTGTGCCGGGAAAAATTCAGATCTTTGTGGTTGAAGACGCTGCTGCTCCAGGACCCT CTGAACCACGACCTCGAGTranslation: (SEQ ID NO:415)MFTVFLLVVLATTVVSVTSYRASHGRKDAADLSAINDNNNCCHPACAGKN SDLCG Toxin Sequence:(SEQ ID NO:416) Cys-Cys-Asn-His-Xaa3-Ala-Cys-Ala-Gly-Lys-Asn-Ser-Asp-Leu-Cys-# Name:     MII[YHT] Species:  magus Toxin Sequence: (SEQ IDNO:417) Gly-Cys-Cys-Xaa5-His-Xaa3-Thr-Cys-His-Leu-Xaa1-His-Ser-Asn-Leu-Cys-# Name:     Nb1.1 Species:  nobilis Cloned:   YesDNA Sequence: (SEQ ID NO:418)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTTGTTTCCTTCACTTCAGATCGTGCATCTGATGGCAGGAATGCCGCAGCCAAAGCTTCTGACCTGATTGCTTTGACCGTCAGGGGATGCTGTGAGCGACCTCCCTGTCGCTGGCAAAATCCAGATCTTTGTGGTGGAAGGCGCTGANATTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:419)MFTVFLLVVLATTVVSFTSDRASDGRNAAAKASDLIALTVRGCCERPPCR WQNPDLCGGRR ToxinSequence: (SEQ ID NO:420)Gly-Cys-Cys-Xaa1-Arg-Xaa3-Xaa3-Cys-Arg-Xaa4-Gln-Asn-Xaa3-Asp-Leu-Cys-Gly-# Name:     Ak1.1 Species:  atlanticusCloned:   Yes DNA Sequence: (SEQ ID NO:421)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACAGTCGTTTCCTTCACTTCAGATAGTGCATTTGATAGCAGGAATGTCGCAGCCAACGACAAAGTGTCTGACATGATCGCTCTGACCGCCAGGAGAACATGCTGTTCCCGTCCTACCTGTAGAATGGAATATCCAGAACTTTGTGGTGGAAGACGCTGATACTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:422)MFTVFLLVVLATTVVSFTSDSAFDSRNVAANDKVSDMIALTARRTCCSRP TCRMEYPELCGGRR ToxinSequence: (SEQ ID NO:423) Thr-Cys-Cys-Ser-Arg-Xaa3-Thr-Cys-Arg-Met-Xaa1-Xaa5-Xaa3-Xaa1-Leu-Cys-Gly-# Name:     Qc1.2 Species:  quercinusCloned:   Yes DNA Sequence: (SEQ ID NO:424)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAATCACGGTGGTTTCCTTCACCTCAGATCATGCATCTGATGGCAGGAATACCGCAGCCAACGACAAAGCGTCTAAACTGATGGCTCTTACGAACGAATGCTGTGACAATCCTCCGTGCAAGTCGAGTAATCCAGATTTGTGTGACTGGAGAAGCTGATGCTCCAGGACCCTNTGAACCACGACCTCGAG Translation: (SEQ ID NO:425)MFTVFLLVVLAITVVSFTSDHASDGRNTAANDKASKLMALTNECCDNPPC KSSNPDLCDWRS ToxinSequence: (SEQ ID NO:426)Asn-Xaa1-Cys-Cys-Asp-Asn-Xaa3-Xaa3-Cys-Lys-Ser-Ser-Asn-Xaa3-Asp-Leu-Cys-Asp-Xaa4-Arg-Ser-{circumflex over ( )}Name:     Lp1.1 Species:  leopardus Cloned:   Yes DNA Sequence: (SEQ IDNO:427) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACGGTCGTTTCCCTCACTTTAGATCGTGCATCTGGTGGCAGGAGATCTGGAGCCGACAACATGATTGCTCTTCTGATCATCAGAAAATGCTGTTCCAATCCCGCCTGTAACAGGTATAATCCAGCAATTTGTGATTGAAGACGCTAATGCTCCAGGAC CCTCTGAACCACGACCTCGAGTranslation: (SEQ ID NO:428)MFTVFLLVVLATTVVSLTLDRASGGRRSGADNMIALLIIRKCCSNPACNR YNPAICD ToxinSequence: (SEQ ID NO:429) Cys-Cys-Ser-Asn-Xaa3-Ala-Cys-Asn-Arg-Xaa5-Asn-Xaa3-Ala-Ile-Cys-Asp-{circumflex over ( )} Name:     Em1.1Species:  emaciatus Cloned:   Yes DNA Sequence: (SEQ ID NO:430)GGATCCATGTTCACCGTGTTTCTGTTGGTTCTCTTGCCAACCACTGTCACTTTACATCGTGCATCTAATGGCAGGAATGCCGCAGCCAGCAGGAAAGCGTCTGCCCTGATCGCTCAGATCGCCGGTAGAGACTGCTGTAACTTTCCTGCTTGTGCCGCGAGTAATCCAGGCCTTTGTACTTGAAGACGCTGCTGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:431)MFTVFLLVLLATTVTLHRASNGRNAAASRKASALIAQIAGRDCCNEPACA ASNPGLCT ToxinSequence: (SEQ ID NO:432)Asp-Cys-Cys-Asn-Phe-Xaa3-Ala-Cys-Ala-Ala-Ser-Asn-Xaa3-Gly-Leu-Cys-Thr-{circumflex over ( )} Name:     C. victor alphaSpecies:  victor Cloned:   Yes DNA Sequence: (SEQ ID NO:433)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACCATCGTTTCCTCCACTTTAGATCGTGCATCTGATGGCATGAATGCTGCAGCGTCTGACCTGATCGCTCTGAGCATCAGGAGATGCTGTTCTTCTCCTCCCTGTTTCGCGAGTAATCCAGCTTGTGGTAGACGACGCTGATGCTCCAGGACCCTCTG AACCACGACCTCGAGTranslation: (SEQ ID NO:434)MFTVFLLVVLATTIVSSTLDRASDGMNAAASDLIALSIRRCCSSPPCFAS NPACGRRR ToxinSequence: (SEQ ID NO:435) Cys-Cys-Ser-Ser-Xaa3-Xaa3-Cys-Phe-Ala-Ser-Asn-Xaa3-Ala-Cys-# Name:     Cj1.1 Species:  cinereus gubba Cloned:   YesDNA Sequence: (SEQ ID NO:436)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCCTGGCAACCACTATCGTTTCCTCCACTTCAGGTCATGCATTTGATGGCAGGAATGCTGCAGCCGACTACAAAGGGTCTGAATTGCTTGCTATGACCGTCAGGGGAGGATGCTGTTCCTTTCCTCCCTGTATCGCAAATAATCCTTTTTGTGCTGGAAGACGCTGATGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:437)MFTVFLLVVLATTIVSSTSGHAFDGRNAAADYKGSELLAMTVRGGCCSFP PCIANNPFCAGRR ToxinSequence: (SEQ ID NO:438)Gly-Gly-Cys-Cys-Ser-Phe-Xaa3-Xaa3-Cys-Ile-Ala-Asn-Asn-Xaa3-Phe-Cys-Ala-# Name:     Fd1.1 Species:  flavidus Cloned:   YesDNA Sequence: (SEQ ID NO:439)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTCGCATCCTCTGTCACTTTAGATCGTGCATCTCATGGCAGGTATATCCCAGTCGTCGACAGAGCGTCTGCCCTGATGGCTCAGGCCGACCTTAGAGGTTGCTGTTCCAATCCTCCTTGTTCCTATCTTAATCCAGCCTGTGGTTAAAGACGCTGCCGCTCCAGGAC CCTCTGAACCACGACCTCGAGTranslation: (SEQ ID NO:440)MFTVFLLVVFASSVTLDRASHGRYIPVVDRASALMAQADLRGCCSNPPCS YLNPACG ToxinSequence: (SEQ ID NO:441)Gly-Cys-Cys-Ser-Asn-Xaa3-Xaa3-Cys-Ser-Xaa5-Leu- Asn-Xaa3-Ala-Cys-#Name:     Em1.2 Species:  emaciatus Cloned:   Yes DNA Sequence: (SEQ IDNO:442) GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTCGCATCCTCTGTCACTTTAGATCGTGCATCTCATGGCAGGTATGCCGCAGTCGTCAACAGAGCGTCTGCCCTGATGGCTCATGCCGCCCTTCGAGATTGCTGTTCCGATCCTCCTTGTGCTCATAATAATCCAGACTGTCGTTAAAGACGCTGCTGCTCCAGGAC CCTCTGAACCACGACCTCGAGTranslation: (SEQ ID NO:443)MFTVFLLVVFASSVTLDRASHGRYAAVVNRASALMAHAALRDCCSDPPCA HNNPDCR ToxinSequence: (SEQ ID NO:444)Asp-Cys-Cys-Ser-Asp-Xaa3-Xaa3-Cys-Ala-His-Asn-Asn-Xaa3-Asp-Cys-Arg-{circumflex over ( )} Name:     Ge1.1Species:  generalis Cloned:   Yes DNA Sequence: (SEQ ID NO:445)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACTACTGTCGTTTCCTTCACTTCAGATCGTGGGTCTGATGGCAGGAATGCCGCAGCCAAGGACAAAGCGTCTGACCTGGTCGCTCTGACCGTCAAGGGATGCTGTTCTAATCCTCCCTGTTACGCGAATAATCAAGCCTATTGTAATGGAAGACGCTGATGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:446)MFTVFLLVVLATTVVSFTSDRGSDGRNAAAKDKASDLVALTVKGCCSNPP CYANNQAYCNGRR ToxinSequence: (SEQ ID NO:447)Gly-Cys-Cys-Ser-Asn-Xaa3-Xaa3-Cys-Xaa5-Ala-Asn-Asn-Gln-Ala-Xaa5-Cys-Asn-# Name:     Wi1.1 Species:  wittigiCloned:   Yes DNA Sequence: (SEQ ID NO:448)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCCTGGCAACCACTGTCGTTTCCCCCACTAGAGATCGTGCATCTGGTGTCAGGAATGTTGTTGCAACAAGCTTTCAGACTCTGACCCACGATGAATGCTGTGCACACCCTTCCTGTTGGAAGGCCGAAGACCTGATTTGTACTAATCAACGTCGCAGGACCCTCTGAAC CACGACCTCGAGTranslation: (SEQ ID NO:449)MFTVFLLVVLATTVVSPTRDRASGVRNVVATSFQTLTHDECCAHLPSCWK AEDLICTNQRRRTL ToxinSequence: (SEQ ID NO:450)Asp-Xaa1-Cys-Cys-Ala-His-Xaa3-Ser-Cys-Xaa4-Lys-Ala-Xaa1-Asp-Leu-Ile-Cys-Thr-Asn-Gln-Arg-Arg-Arg- Thr-Leu-{circumflexover ( )} Name:     Ca1.5 Species:  caracteristicus Cloned:   Yes DNASequence: (SEQ ID NO:451)GGATCCATGTTCACCGTGTTTCTGTTGGTTGTCTTGGCAACCACTGTCGTTTCCTTCACTTCAGATCGTGCGTCTGAAGGCAGGAATGCTGCAGCCAAGGACAAAGCGTCTGACCTGGTGGCTCTGAGAGTCAGGGGATGCTGTGCCATTCGTGAATGTCGCTTGCAGAATGCAGCGTATTGTGGTGGAATATCCTGATGCTCCAGGACCCTCTGAACCACGACCTCGAG Translation: (SEQ ID NO:452)MFTVFLLVVLATTVVSFTSDRASEGRNAAAKDKASDLVALRVRGCCAIRE RLQNAAYCGGIS ToxinSequence: (SEQ ID NO:453)Gly-Cys-Cys-Ala-Ile-Arg-Xaa1-Cys-Arg-Leu-Gln-Asn-Ala-Ala-Xaa5-Cys-Gly-Gly-Ile-Ser-{circumflex over ( )} Name:     Bt1.10Species:  betulinus Cloned:   Yes DNA Sequence: (SEQ ID NO:454)AGTAATTNATATANNAGAAAGNAANANAAAANNATANAGAATTTAAGTAATNTAAGAANGAGANAGTGAATAGNAGNTAAGTAGANNAAGANAGGTAGANAGNANANGNGGANGNTAGNTAATAGATANNNTATNGAGANATTANTAGCNGTATANANAAGAAAAGAGGGNAANNGAAATGNNGNAANNATAANTANTANNGATNGANNNGNAAGTGNNAAGNGTANAAGGAANAACAAANTNGTTGTNTAATNTGNNTGNGTGTGTNTGTGTGNGTGTGTGTGTGTGNGTGNGTGTGTNTGTGNGNNTGTGTGNGNGNGNGNGTGTGTGTGTGNGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGNGTGTGTGGTTCTGGATCCAGCATCTGGTGGCAGGAAGGCTGCAGCCAAAGCGTCTAACCGGATCGCTCTGACCGTCAGGAGTGCAACATGCTGTTATTATCCTCCCTGTTACGAGGCTTATCCAGGGTTGTCTGTAACGTGAATCATCCAGACCTTTGTGGCTGAAGACCCTGATGCTC CAGGGGCAAGTTCAATranslation: (SEQ ID NO:455) SGGRKAAAKASNRIALTVRSATCCYYPPCYEAYPESCLToxin Sequence: (SEQ ID NO:456)Ser-Ala-Thr-Cys-Cys-Xaa5-Xaa5-Xaa3-Xaa3-Cys-Xaa5-Xaa1-Ala-Xaa5-Xaa3-Xaa1-Ser-Cys-Leu-{circumflex over ( )}

[0087] TABLE 2 Alignment of γ-Conopeptides¹ (SEQ ID NO:) 4/43 SNX-------DCRGYDAPCSSGAPCCDWWTCS (457) ARTNRCF{circumflex over ( )} Af6.1GMW---GDCKDGLTTCFAPSECCSE-DC- (458) E-GS-CTMW{circumflex over ( )} At6.2---WREGSCTSWLATCTQDQQCCTD-VDY (459) KRDY-CALWDDR{circumflex over ( )}Af6.3 ----N---CSDDWQYCESPSDCCSW-DC- (460) D-VV-CS# Af6.4--WWRWGGCMAWFGKCSKDSECCSN-SC- (461) DITR-CELMRFPPDW{circumflex over ( )}At6.5 -------DCRGYDAPCSSGAPCCDWWTCS (462) ARTGRCF{circumflex over ( )}At6.6 ---L----CPDYTEPCSHAHECCSW-NC- (463) HNGH-CT# g-PnVIIA--------DCTSWFGRCTVNSXCCSN-SC (465) -DQTY-CXLYAFOS{circumflex over ( )}²Gm6.7 --------ECRAWYAPCSPGAQCCSLLMC (466) SKATSRCILAL{circumflex over( )}² J010 --------CKTYSKYCXADSXCCTX-QC- (467) VRSY-CTLF#² Mr6.1----N-GQCEDVWMPCTSNWXCCSL-DC- (468) E-MY-CTQI#² Mr6.2--------CGGWSTYCEVDEXCCSE-SC- (469) VRSY-CTLF#² Mr6.3----N-GGCKATWMSCSSGWXCCSM-SC- (470) D-MY-C#² R6.10--UFGHXXCTYULGPCXVDDTCCSA-SC- (471) XSKF-CGLU{circumflex over ( )} R6.9--WWE-GECSNWLGSCSTPSNCCLK-SC- (472) N-GH-CTLW{circumflex over ( )} Tx6.1---L----CODYTXOCSHAHXCCSW-NC- (473) YNGH-CT#² Tx6.14-------DCYSWLGSCIAPSQCCSE-VC- (474) D-YY-CRLWR{circumflex over ( )}Tx6.4 --WL---ECSVWFSHCTKDSXCCSN-SC- (475) DQTY-CTLMPPDW{circumflex over( )}² Tx6.5 GMW---GECKDGLTTCLAPSXCCSE-DC- (476) E-GS-CTMW{circumflexover ( )}² Tx6.6 D-WWD-DGCSV-WGPCTVNAXCCSG-DC- (477)H-ET-CIFGWEV{circumflex over ( )}² Tx6.9 --WWRWGGCMAWFGLCSRDSXCCSN-SC-(478) DVTR-CELMPFPPDW{circumflex over ( )}² TxVIIA--------CGGYSTYCXVDSXCCSD-NC- (479) VRSY-CTLF#

[0088] TABLE 3 Alignment of σ-Conopeptides (SEQ ID NO:) Ca8.1GCS-GT-CHRREDGKC-RGTCDCSG-YSYCR (480) CG-DAHHFYRGCTCSCQ# Ca8.2GCSG-T-CHRREDGKC-RGTCDCSG-YSYCR (481) CG-DAHHFYRGCTCTC{circumflex over( )} Ca8.3 GCSG-T-CRRHRDGKC-RGTCDCSG-YSYCR (482)CG-DAHHFYRGCTCTC{circumflex over ( )} Ca8.4GCSG-T-CRRHRDGKC-RGTCDCSG-YSYCR (483) CG-DAHHFYRGCTCTC{circumflex over( )} Ca8.5 GCSG-T-CHRREDGKC-RGTCDCSG-YSYCR (484)CG-DAHHFYRGCTCTC{circumflex over ( )} Ca8.6GCSG-T-CHRRQNGEC-QGTCDCDG-HDHCD (485) CG-DTLGTYSGCVCIC{circumflex over( )} La8.1 QSE--TACRSLGWYQCM-GKCQ-LGVHSWCE (486)CIYNRGSQKSGCACRCQK{circumflex over ( )} Mn8.1QCTLVNNCDRNGERACN-GDCSCEGQI--CK (487) CGYRVSPGKSGCACTCRNAK{circumflexover ( )} P8.1 GCS-GSPCFKNKT--C-RDECICGG-LSNCW (488)CGY-GGS--RGCKCTCRE{circumflex over ( )} R8.1KCNF-DKCKGTGVYNCG-ESCSCEGLHS-CR (489) CTYNIGSMKSGCACICTYY{circumflexover ( )} R8.2 YGLGCA-GT-CGSSSN--CVRDYCDC-P-KP (490)NCYCT-GKGFRQPGCGCSCL# Sx8.1 QCTFVNNCQQNG--CAN-GDCSCGDQI--CK (491)CGYRISPGRSGCACTCRNAK{circumflex over ( )} T8.1FGPIC---T-CFKSQN--C-RGSCECMS-PP (492) GCYCS-NNGIRERGCSCTCPGT# T8.2GCT--GNCDW----TCS-GDCSCQGTSDSCH (493) CIPPKSIGNR-CRCQCKRKIEID{circumflexover ( )}

[0089] TABLE 4 Alignment of τ-Conopeptides (SEQ ID NO:) Tx5.2a---ECCEDGW-CCTAAPLT#¹ (494) Tx5.2b ---GCCEDGW-CCTAAPLT# (495) Mr5.1--NGCC-RAGDCCSRFEIKENDF#¹ (496) Mr5.3 --NGCC-RAGDCCS{circumflex over( )}¹ (497) Mr5.2 --NACC-IVRQCC{circumflex over ( )}¹ (498) Qc5.1---GCCAR-LTCCV#¹ (499) Qc5.2 ---GCCAM-LTCCV#¹ (500) t-PVA---GCCPKQMRCCTL#¹ (501) Ca5.1 -----CCPRRLACCII#¹ (502) Ca5.2----CCPNK-PCCFI#¹ (503) G5.1 -ZGWCCKENIACCI{circumflex over ( )}¹ (504)G5.2 -ZGWCCKENIACCV{circumflex over ( )}¹ (505) Im5.1 DWNSCCGKNPGCCPW#¹(506) Bt5.1 ---NCCPDSPPCCH{circumflex over ( )} (507) Af5.2--GNCCEFWEFCCD{circumflex over ( )} (508) Da5.1 ----CCEYWKLCC# (509)Om5.1 ---VCCGYKFFCCR{circumflex over ( )} (510) t-AuVA---FCCPVIRYCCW{circumflex over ( )}¹ (511) t-AuVB---FCCPFIRYCCW{circumflex over ( )}¹ (512) Au5.1----CCPMIYWCCS{circumflex over ( )} (513) Au5.4----CCPEIYWCCS{circumflex over ( )} (514) Nb5.1 ---ICCPIILWCC# (515)Af5.1 ----CCPPVIWCC# (516) Tx5.1 -----CCQTFYWCCVQ# (517) Au5.3WNNYCCTNELWCC# (518) Gm5.1 ---LCCVTEDWCCEWW{circumflex over ( )}¹ (519)Gm5.2 ---VCCRPVQDCCS#¹ (520) Da5.2 -PVNCCPIDQSCCS{circumflex over ( )}(521) Sf5.1 GNIHCCTKYQPCCSSPS{circumflex over ( )} (522)

[0090] TABLE 5 Alignment of Mar-Type Conopeptides¹ (SEQ ID NO:) Tx1.6(Q819) -ZTCCGYRMCVPC# (523) Bn1.5 (Q818) -A-CCGYKLCSPC{circumflex over( )} (524) Pn1.3 (Q820) -STCCGFKMCIPCR{circumflex over ( )} (525) Pn1.5(AA200) -STCCGFKMCIPCS{circumflex over ( )} (526) Pn1.7 (AA456)-STCCGFKMCIPC# (527) Ep1.5 (AA457) -STCCGYRMCVPC# (528) Mr1.3NGVCCGYKLCLPC{circumflex over ( )} (529) Pn1.6 (AA390)--LCCGFWMCIPCN{circumflex over ( )} (530) Mr1.1 NGVCCGYKLCHOC{circumflexover ( )} (531) Mr1.2 -GVCCGYKLCHOC{circumflex over ( )} (532) Bn1.5--ACCGYKLCSPC{circumflex over ( )} (533) Au1.4 -SVCCGYKLCFPC# (534)Tx1.7 NGVCCGYRMCVPC# (535) Tx1.6 -ZTCCGYRMCVPC# (536) Af1.3-ZACCGFKMCVPC# (537) Pn1.3 -STCCGFKMCIPCR{circumflex over ( )} (538)Pn1.4 NGVCCGFWMCIPCN{circumflex over ( )} (539) Om1.7-DVCCYVRMC-PCR{circumflex over ( )} (540)

[0091] TABLE 6 Alignment of Contryphans* (SEQ ID NO:) Contryphan-ImZ--C-GQAWC# (541) Contryphan-Sm-dW4, V7 GCOWQPVC# (542) Contryphan-Ar-1ZYGCOOGLWCH{circumflex over ( )} (543) C. arenatus contryphanASGCPWRPWC# (544) 1A C. arenatus contryphan 2 ZYGCPVGLWCD{circumflexover ( )} (545) C. arenatus contryphan 4 SGCPWQPWC# (546) C. arenatuscontryphan 1 SGCPWHPWC# (547)

[0092] TABLE 7 Alignment of αA-Conopeptides* (SEQ ID NO:) αA-GCCGKYONAACHOCGCTVGROOYCDROSGG# (548) EIVB P4.1GCCGSYPNAACHPCGCK-DRPSYCGQ# (549) P4.2 EGCC---SNPACHPCGCK-DRPSYCGQ#(550)

[0093] TABLE 8 Alignment of Bromosleeper Conopeptides* (SEQ ID NO:)Bromosleeper-Ar1 VVTEACEESCEEEEKHCCH (551) VNNGVPSCAVICW#Bromosleeper-Ar1A IVTEACEESCEDEEKHCCH (552) VNNGVPSCAVICW#Bromosleeper-Ar2 IVTEACEEHCEDEEQFCCG (553) LENGQPFCAPVCF#Bromosleeper-Ar3 VVTGACEEHCEDEEKHCCG (554) LENGQPFCARLCL#Bromosleeper-Di1 NVDQECIDACQLEDKNCCG (555) RTDGEPRCAKICL#Bromosleeper-Di2 ETDQECIDICKQEDKKCCG (556) RSNGEPTCAKICL#Bromosleeper-Di3 ETDQECIDTCEQEDKKCCG (557) RTNGEPVCAKICF#Bromosleeper-P1 PKTEACEEVCELEEKHCCC (558) IRSDGPKCSRKCLLSIFC{circumflexover ( )} Bromosleeper-P2 VVSEECKKYCKKQNKNCCS (559) SKHEEPRCAKICF#Bromosleeper-Sn AVTEACTEDCKTQDKKCCG (560) EMNGQHTCAKICL# Bromosleeper-T1PKTKECERYCELEEKHCCC (561) IRSNGPKCSRICIFKFWC{circumflex over ( )}Bromosleeper-T2 PKTRECEMQCEQEEKHCCR (562) VRDGTGQCAPKCLGINW{circumflexover ( )}

[0094] TABLE 9 Alignment of Conopressins (SEQ ID NO:) Conopressin-GCFIRNCPKG# (563) Conopressin-S CIIRNCPRG# (564)

[0095] TABLE 10 Alignment of O-Superfamily (SEQ ID NO:) Ar6.1-----GCTPPGGVCGYHGH---CCD-F-C--DTFGNLCVS# (565) C. geogr. GS-A-----ACSGRGSRCPPQ-----CCMGLTC--GREYPPRC# (566) Ca6.3 (F166)-----NCGEQGEGCAT--RP--CCSGLSC-VGSRPGGLCQY# (567) convulsion-----NCPY----CVVY-----CCPPAYCEASG-----CRPP# (568) De6.1-----ACKOKNNLCAITXMAX-CCSGF-CLIY-----RC{circumflex over ( )} (569) Lv6.2(I16) -----SCGHSGAGCYT--RP--CCPGLHC-SGGQAGGLCV{circumflex over ( )}(570) Lv6.3 (I12)-----DCGESGQGCYSV-RP--CCPGLICKGTG-GGGLCRPSGI{circumflex over ( )} (571)Mi6.1 (F204)      ------CTPPGGLC-YHAYP--CCSKT-C---NLDTSQCEPRWS{circumflex over ( )}(572) Mi6.2 (F162)      ------CTDDSQFCNPSNHD--CCSG-KCIDEGDNG-ICAIVPENS{circumflex over( )} (573) Mi6.3 (F161)      ------CTEDSQFCNPSNHD--CCSG-KCIDEGDNG-ICAIVPENS{circumflex over( )} (574) Pu6.1 (JG14)      ------CSDFGSDCVPATHN--CCSG-ECFGFEDFG-LCT{circumflex over ( )}(575) Qc6.4 (F025)      -----ACSQVGEACFPQ-KP--CCPGFLC--NH-IGGMCHH{circumflex over ( )}(576) S6.4       ------CLPDGTSCLFSRIR--CCGT--C---SSILKSCVS{circumflexover ( )} (577) Ts6.3 (F081)      -----SCAEFGEVC-SS-TA--CCPDLDCVEAYSP--ICLWE{circumflex over ( )}(578) Tx6.3       -----KCVEQWKYCTR---ESLCCAGL-CLFS-----FCIL{circumflexover ( )} (579) Tx6.7      ------CVEQWEVCGIILFSSSCCGQL-CLFG-----FCVL{circumflex over ( )}(580) Vr6.1 (F198)      -----DCGGQGEGCYT--QP--CCPGLRCRGGGTGGGVCQL{circumflex over ( )}(581) Wi6.1 (M406)      FGSFIPCARLGEPC-----T-ICCRPLRCRESG--TPTCQV{circumflex over ( )}(582) Rg6.6 (K861)      -----TCLEHNKLCWYD---RDCCTIY-C---N--ENKCGVKPQ{circumflex over ( )}(583) EST202       -----ACKSNYDCPQRFKCCSYTWNGSSGYCKRVCYLYR{circumflexover ( )} (584)

[0096] TABLE 11 Alignment of ψ-Conopeptides* (SEQ ID NO:) ψ-PIIIFGOOCCLYGSCROFOGCYNALCCRK# (585) U021 HPPCCMYGRCRRYPGCSSASCCQG# (586)homolog

[0097] TABLE 12 Alignment of kappaA-Conopeptides* (SEQ ID NO:) Cn10.3(J454) APELVVTATTTCCGYDPMTICPPCMCTHS (587) CPPKRKP# A10.2 (H350)ZSWLVPSTITTCCGYDPGTMCPPCRCNNT (588) CKPKKPKPGK# Cn10.4 (G851)APELVVTATTTCCGYDPMTWCPSCMCTYS (589) CPHQRKKP# M10.3 (X003)APELVVTATTTCCGYDPMTICPPCMCTHS (590) CPPKGKP# A10.3 (AA400)ZKWLVHSKITYCCGYNKMDMCPPCMCTYS (591) CPPLKKKRP# A10.4 (AA401)APWTVVTATTNCCGITGPG-CLPCRCTQT (592) C#

[0098] TABLE 13 Alignment of α-Conopeptides (SEQ ID NO:) G1.4-ECCHPACGKHYSC# (593) G1.5 -ECCNPACGRHFSC# (594) S1.8AYCCHPACGPNYSCGTSCSRTL{circumflex over ( )} (595) S1.9 AYCCHPVCGKNFDC#(596) Ra1.1 GCCCNPACGPNYGCGTSCSRTL{circumflex over ( )} (597) Ar1.1ZDYCCTIPSCWDRYKERCRHIR{circumflex over ( )} (593) Er1.1ZDYCCTIPSCWDRYKERCRHIR{circumflex over ( )} (599) Mi1.2-DYCCHRGPCMVW----C# (600) Jp1.1 --GCCSDPRC--RYR--CR{circumflex over ( )}(601) a-OmIA --GCCSHPACNVNNPHICG# (602) a-OmIA [COOH]--GCCSHPACNVNNPHICG{circumflex over ( )} (603) Qc1.1Z-GCCSDPACAVSNPDICGG# (604) Bn1.6 PE-CCTHPACHVSHPELC# (605) Mr1.5PE-CCTHPACHVSNPELC# (606) Mi1.1 ---CCNHPACAGKNSDLC# (607) MII [YHT]--GCCYHPTCHLEHSNLC# (608) Nb1.1 --GCCERPPCRWQNPDLCG# (609) Ak1.1--TCCSRPTCRMEYPELCG# (610) Qc1.2 NE-CCDNPPCKSSNPDLCDWRS{circumflex over( )} (611) Lp1.1 ---CCSNPACNRYNPAICD{circumflex over ( )} (612) Em1.1-D-CCNFPACAASNPGLCT{circumflex over ( )} (613) C. victor alpha---CCSSPPCFASNPA-C# (614) Cj1.1 -GGCCSFPPCIANNPF-CA# (615) Fd1.1--GCCSNPPCSYLNPA-C# (616) Em1.2 -D-CCSDPPCAHNNPD-CR{circumflex over ( )}(617) Ge1.1 --GCCSNPPCYANNQAYCN# (618 Wi1.1DE-CCAHPSCWKAEDLICTNQRRRTL{circumflex over ( )} (619) Ca1.5--GCCAIRECRLQNAAYCGGIS{circumflex over ( )} (620) Bt1.10SATCCYYPPCYEAYPESCL{circumflex over ( )} (621)

[0099] TABLE 14 Alignment of Conopeptides* (SEQ ID NO:) ConvulsantVYXTHP{circumflex over ( )} (622) WG002 WSWRMGNGDRRSDQ{circumflex over( )} (623) QcII DCQPCGHNVCC{circumflex over ( )} (624) Scratching,KFLSGGFKXIVCHRYCAKGIAKEFCNCPD# (625) Convulsion MAG-1 RPKNSW{circumflexover ( )} (626) MAG-2 AROKNSW$ (627) MAG-3 ROKNSW{circumflex over ( )}(628) EST66 CCPSSKEDSLNCIETMATTATCMKSNKGEIY (629)SYACGYCGKKKESCFGDKKPVTDYQCQTRNI PNPCGGAAL{circumflex over ( )} G12.2DESKCDRCNCAELRSSRCTQAIFCLTPELCT (630) PSISCPTGECRCTKFHQSRCTRFVECVPNKCRDA{circumflex over ( )} G12.1 DDSYCDGCLCTILKKETCTSTMSCRGT- CR (631)KEWPCWEEDCYCTEIQGGACVTPSECKPGEC {circumflex over ( )} EST171GCVYEGIEYSVGETYQADCNTCRCDGFDLAT (632) CTVAGCTGFGPE{circumflex over ( )}U010 homolog SGPADCCRMKECCTDRVNECLQRYSGREDKF (633)VSFCYQEATVTCGSFNEIVGCCYGYQMCMIR VVKPNSLSGAHEACKTVSCGNPCA{circumflex over( )} P29 DCCGVKLEMCHPCLCDNSCKNYGK# (634) EST87GEPIPTTVINYGECCKDPSCWVKVKDFQCPG (635) ASPPN{circumflex over ( )} Ge3.1(F590) QCCTFCNFGCQPCCVP{circumflex over ( )} (636) Ts10.1DGCPPHPVPGMHKCMCTNTC (637) Conophysin-R HPTKPCMYCSFCQCVGPHICCGPTGCEMGTA(638) EANMCSEEDEDPIPCQVFGSDCALNNPDNIH GHCVADGICCVDDTCTTHLGCL{circumflexover ( )}

[0100] It will be appreciated that the methods and compositions of theinstant invention can be incorporated in the form of a variety ofembodiments, only a few of which are disclosed herein. It will beapparent to the artisan that other embodiments exist and do not departfrom the spirit of the invention. Thus, the described embodiments areillustrative and should not be construed as restrictive.

List of References

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1 638 1 290 DNA Conus ammiralis CDS (4)..(231) 1 atc atg gag aaa ctg ataatt ctg ctt ctt gtt gct gct gta ctg atg 48 Met Glu Lys Leu Ile Ile LeuLeu Leu Val Ala Ala Val Leu Met 1 5 10 15 tcg acc cag gcc ctg gtt gaacgt gct gga gaa aac cgc tca aag gag 96 Ser Thr Gln Ala Leu Val Glu ArgAla Gly Glu Asn Arg Ser Lys Glu 20 25 30 aac atc aat ttt tta tta aaa agaaag aga gct gct gac agg ggg atg 144 Asn Ile Asn Phe Leu Leu Lys Arg LysArg Ala Ala Asp Arg Gly Met 35 40 45 tgg ggc gat tgc aaa gat ggg tta acgaca tgt ttt gcg ccc tca gag 192 Trp Gly Asp Cys Lys Asp Gly Leu Thr ThrCys Phe Ala Pro Ser Glu 50 55 60 tgt tgt tct gag gat tgt gaa ggg agc tgcacg atg tgg tgatgacctc 241 Cys Cys Ser Glu Asp Cys Glu Gly Ser Cys ThrMet Trp 65 70 75 tgaccacaag ccatctgaca tcaccactct cctcttcaga ggcttcaag290 2 76 PRT Conus ammiralis 2 Met Glu Lys Leu Ile Ile Leu Leu Leu ValAla Ala Val Leu Met Ser 1 5 10 15 Thr Gln Ala Leu Val Glu Arg Ala GlyGlu Asn Arg Ser Lys Glu Asn 20 25 30 Ile Asn Phe Leu Leu Lys Arg Lys ArgAla Ala Asp Arg Gly Met Trp 35 40 45 Gly Asp Cys Lys Asp Gly Leu Thr ThrCys Phe Ala Pro Ser Glu Cys 50 55 60 Cys Ser Glu Asp Cys Glu Gly Ser CysThr Met Trp 65 70 75 3 31 PRT Conus ammiralis PEPTIDE (1)..(31) Xaa atresidues 18, 22 and 25 is Glu or gamma-carboxy-Glu; Xaa at residue 16 isPro or hydroxy-Pro; Xaa at residues 3 and 31is Trp (D or L) or bromo-Trp(D or L) 3 Gly Met Xaa Gly Asp Cys Lys Asp Gly Leu Thr Thr Cys Phe AlaXaa 1 5 10 15 Ser Xaa Cys Cys Ser Xaa Asp Cys Xaa Gly Ser Cys Thr MetXaa 20 25 30 4 295 DNA Conus ammiralis CDS (4)..(246) 4 atc atg gag aaactg aca att ctg ctt ctt gtt gct gct gta ctg atg 48 Met Glu Lys Leu ThrIle Leu Leu Leu Val Ala Ala Val Leu Met 1 5 10 15 tcg acc cag gcc ctgcct caa ggt ggt gga gaa aaa cgc cca agg gag 96 Ser Thr Gln Ala Leu ProGln Gly Gly Gly Glu Lys Arg Pro Arg Glu 20 25 30 aat atc aga ttt tta tcaaaa aga aag aca aat gct gag cgt tgg agg 144 Asn Ile Arg Phe Leu Ser LysArg Lys Thr Asn Ala Glu Arg Trp Arg 35 40 45 gag ggc agt tgc acc tct tggtta gcg acg tgt acg caa gac cag caa 192 Glu Gly Ser Cys Thr Ser Trp LeuAla Thr Cys Thr Gln Asp Gln Gln 50 55 60 tgc tgt act gat gtt tgt tac aaaagg gac tac tgc gcc ttg tgg gat 240 Cys Cys Thr Asp Val Cys Tyr Lys ArgAsp Tyr Cys Ala Leu Trp Asp 65 70 75 gac cgc tgaccacaag ccatctgacatcaccactct cctgttcaga gtcttcaag 295 Asp Arg 80 5 81 PRT Conus ammiralis5 Met Glu Lys Leu Thr Ile Leu Leu Leu Val Ala Ala Val Leu Met Ser 1 5 1015 Thr Gln Ala Leu Pro Gln Gly Gly Gly Glu Lys Arg Pro Arg Glu Asn 20 2530 Ile Arg Phe Leu Ser Lys Arg Lys Thr Asn Ala Glu Arg Trp Arg Glu 35 4045 Gly Ser Cys Thr Ser Trp Leu Ala Thr Cys Thr Gln Asp Gln Gln Cys 50 5560 Cys Thr Asp Val Cys Tyr Lys Arg Asp Tyr Cys Ala Leu Trp Asp Asp 65 7075 80 Arg 6 36 PRT Conus ammiralis PEPTIDE (1)..(36) Xaa at residue 3 isGlu or gamma-carboxy-Glu; Xaa at residues 1, 9 and 33 is Trp (D or L) orbromo-Trp (D or L); Xaa at residues 25 and 29 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 6 Xaa Arg XaaGly Ser Cys Thr Ser Xaa Leu Ala Thr Cys Thr Gln Asp 1 5 10 15 Gln GlnCys Cys Thr Asp Val Cys Xaa Lys Arg Asp Xaa Cys Ala Leu 20 25 30 Xaa AspAsp Arg 35 7 275 DNA Conus ammiralis CDS (4)..(219) 7 atc atg cag aaactg ata att ctg ctt ctt gtt gct gct gtg ctg atg 48 Met Gln Lys Leu IleIle Leu Leu Leu Val Ala Ala Val Leu Met 1 5 10 15 tcg acc cag gcc ctgttt caa gaa aaa cgc aca atg aag aag atc gat 96 Ser Thr Gln Ala Leu PheGln Glu Lys Arg Thr Met Lys Lys Ile Asp 20 25 30 ttt tta tca aag gga aaggca gat gct gag aag cag agg aag cgc aat 144 Phe Leu Ser Lys Gly Lys AlaAsp Ala Glu Lys Gln Arg Lys Arg Asn 35 40 45 tgc tcg gat gat tgg cag tattgt gaa agt ccc agt gac tgc tgt agt 192 Cys Ser Asp Asp Trp Gln Tyr CysGlu Ser Pro Ser Asp Cys Cys Ser 50 55 60 tgg gat tgt gat gtg gtc tgc tcggga tgaactctga ccacaagtca 239 Trp Asp Cys Asp Val Val Cys Ser Gly 65 70tccgacatca ccactctcct gttcagaggc ttcaag 275 8 72 PRT Conus ammiralis 8Met Gln Lys Leu Ile Ile Leu Leu Leu Val Ala Ala Val Leu Met Ser 1 5 1015 Thr Gln Ala Leu Phe Gln Glu Lys Arg Thr Met Lys Lys Ile Asp Phe 20 2530 Leu Ser Lys Gly Lys Ala Asp Ala Glu Lys Gln Arg Lys Arg Asn Cys 35 4045 Ser Asp Asp Trp Gln Tyr Cys Glu Ser Pro Ser Asp Cys Cys Ser Trp 50 5560 Asp Cys Asp Val Val Cys Ser Gly 65 70 9 25 PRT Conus ammiralisPEPTIDE (1)..(25) Xaa at residue 10 is Glu or gamma-carboxy-Glu; Xaa atresidue 12 is Pro or hydroxy-Pro; Xaa at residues 6 and 18 is Trp (D orL) or bromo-Trp (D or L); Xaa at residue 8 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 9 Asn Cys Ser AspAsp Xaa Gln Xaa Cys Xaa Ser Xaa Ser Asp Cys Cys 1 5 10 15 Ser Xaa AspCys Asp Val Val Cys Ser 20 25 10 280 DNA Conus ammiralis CDS (4)..(246)10 atc atg cag aaa ctg ata atc ctg ctt ctt gtt gct gct cta ctg ttg 48Met Gln Lys Leu Ile Ile Leu Leu Leu Val Ala Ala Leu Leu Leu 1 5 10 15tcg atc cag gcg gta aat caa gaa aaa cac caa cgg gca aag atc aac 96 SerIle Gln Ala Val Asn Gln Glu Lys His Gln Arg Ala Lys Ile Asn 20 25 30 ttgctt tca aag aga aag cca cct gct gag cgt tgg tgg cgg tgg gga 144 Leu LeuSer Lys Arg Lys Pro Pro Ala Glu Arg Trp Trp Arg Trp Gly 35 40 45 gga tgcatg gct tgg ttt ggg aaa tgt tcg aag gac tcg gaa tgt tgt 192 Gly Cys MetAla Trp Phe Gly Lys Cys Ser Lys Asp Ser Glu Cys Cys 50 55 60 tct aat agttgt gac ata acg cgc tgc gag tta atg cga ttc cca cca 240 Ser Asn Ser CysAsp Ile Thr Arg Cys Glu Leu Met Arg Phe Pro Pro 65 70 75 gac tggtgacatcgac actctcctgt tcagagtctt caag 280 Asp Trp 80 11 81 PRT Conusammiralis 11 Met Gln Lys Leu Ile Ile Leu Leu Leu Val Ala Ala Leu Leu LeuSer 1 5 10 15 Ile Gln Ala Val Asn Gln Glu Lys His Gln Arg Ala Lys IleAsn Leu 20 25 30 Leu Ser Lys Arg Lys Pro Pro Ala Glu Arg Trp Trp Arg TrpGly Gly 35 40 45 Cys Met Ala Trp Phe Gly Lys Cys Ser Lys Asp Ser Glu CysCys Ser 50 55 60 Asn Ser Cys Asp Ile Thr Arg Cys Glu Leu Met Arg Phe ProPro Asp 65 70 75 80 Trp 12 39 PRT Conus ammiralis PEPTIDE (1)..(39) Xaaat residues 19 and 31 is Glu or gamma-carboxy-Glu; Xaa at residues 36and 37 is Pro or hydroxy-Pro; Xaa at residues 1, 2, 4, 10 and 39 is Trp(D or L) or bromo-Trp (D or L) 12 Xaa Xaa Arg Xaa Gly Gly Cys Met AlaXaa Phe Gly Lys Cys Ser Lys 1 5 10 15 Asp Ser Xaa Cys Cys Ser Asn SerCys Asp Ile Thr Arg Cys Xaa Leu 20 25 30 Met Arg Phe Xaa Xaa Asp Xaa 3513 267 DNA Conus ammiralis CDS (4)..(213) 13 atc atg gag aaa ctg aca atcctg ctt ctt gtt gct gct gta ctg acg 48 Met Glu Lys Leu Thr Ile Leu LeuLeu Val Ala Ala Val Leu Thr 1 5 10 15 tcg acc cag gcc ctg att caa ggtggt gga gac gaa cgc caa aag gca 96 Ser Thr Gln Ala Leu Ile Gln Gly GlyGly Asp Glu Arg Gln Lys Ala 20 25 30 aag atc aac ttt ctt tca agg tcg gaccgc gat tgc agg ggt tac gat 144 Lys Ile Asn Phe Leu Ser Arg Ser Asp ArgAsp Cys Arg Gly Tyr Asp 35 40 45 gcg ccg tgt agc tct ggc gcg cca tgt tgtgat tgg tgg aca tgt tca 192 Ala Pro Cys Ser Ser Gly Ala Pro Cys Cys AspTrp Trp Thr Cys Ser 50 55 60 gca cga acc ggg cgc tgt ttt taggctgaccacaagccatc cgacatcacc 243 Ala Arg Thr Gly Arg Cys Phe 65 70 actctcctcttcagaggctt caag 267 14 70 PRT Conus ammiralis 14 Met Glu Lys Leu Thr IleLeu Leu Leu Val Ala Ala Val Leu Thr Ser 1 5 10 15 Thr Gln Ala Leu IleGln Gly Gly Gly Asp Glu Arg Gln Lys Ala Lys 20 25 30 Ile Asn Phe Leu SerArg Ser Asp Arg Asp Cys Arg Gly Tyr Asp Ala 35 40 45 Pro Cys Ser Ser GlyAla Pro Cys Cys Asp Trp Trp Thr Cys Ser Ala 50 55 60 Arg Thr Gly Arg CysPhe 65 70 15 29 PRT Conus ammiralis PEPTIDE (1)..(29) Xaa at residues 8and 14 is Pro or hydroxy-Pro; Xaa at residues 18 and 19 is Trp (D or L)or bromo-Trp (D or L); Xaa at residue 5 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 15 Asp Cys Arg Gly Xaa AspAla Xaa Cys Ser Ser Gly Ala Xaa Cys Cys 1 5 10 15 Asp Xaa Xaa Thr CysSer Ala Arg Thr Gly Arg Cys Phe 20 25 16 277 DNA Conus ammiralis CDS(4)..(222) 16 atc atg cag aaa ctg aca att ctg ctt ctt gtt gct gct gtgctg atg 48 Met Gln Lys Leu Thr Ile Leu Leu Leu Val Ala Ala Val Leu Met 15 10 15 tcg acc cag gcc gtg ctt caa gaa aaa cgc cca aag gag aag atc aag96 Ser Thr Gln Ala Val Leu Gln Glu Lys Arg Pro Lys Glu Lys Ile Lys 20 2530 ttt tta tca aag aaa aag aca gat gct gag aag cag cag aag cgc ctt 144Phe Leu Ser Lys Lys Lys Thr Asp Ala Glu Lys Gln Gln Lys Arg Leu 35 40 45tgc ccg gat tac acg gag cct tgt tca cat gcc cat gaa tgc tgt tca 192 CysPro Asp Tyr Thr Glu Pro Cys Ser His Ala His Glu Cys Cys Ser 50 55 60 tggaat tgt cat aat ggg cac tgc acg gga tgaactcgga ccacaagcca 242 Trp AsnCys His Asn Gly His Cys Thr Gly 65 70 tcgacatcat cactctcctg ttcagagtcttcaag 277 17 73 PRT Conus ammiralis 17 Met Gln Lys Leu Thr Ile Leu LeuLeu Val Ala Ala Val Leu Met Ser 1 5 10 15 Thr Gln Ala Val Leu Gln GluLys Arg Pro Lys Glu Lys Ile Lys Phe 20 25 30 Leu Ser Lys Lys Lys Thr AspAla Glu Lys Gln Gln Lys Arg Leu Cys 35 40 45 Pro Asp Tyr Thr Glu Pro CysSer His Ala His Glu Cys Cys Ser Trp 50 55 60 Asn Cys His Asn Gly His CysThr Gly 65 70 18 26 PRT Conus ammiralis PEPTIDE (1)..(26) Xaa atresidues 7 and 14 is Glu or gamma-carboxy-Glu; Xaa at residues 3 and 8is Pro or hydroxy-Pro; Xaa at residue 18 is Trp (D or L) or bromo-Trp (Dor L); Xaa at residue 5 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr,O-sulpho-Tyr or O-phospho-Tyr 18 Leu Cys Xaa Asp Xaa Thr Xaa Xaa Cys SerHis Ala His Xaa Cys Cys 1 5 10 15 Ser Xaa Asn Cys His Asn Gly His CysThr 20 25 19 287 DNA Conus ammiralis CDS (4)..(228) 19 atc atg cag aaactg ata att ctg ctc ctt gtt gct gct gta ctg atg 48 Met Gln Lys Leu IleIle Leu Leu Leu Val Ala Ala Val Leu Met 1 5 10 15 tcg acc cag gcc atgttt caa ggt gat gga gaa aaa tcc cgg aaa gcg 96 Ser Thr Gln Ala Met PheGln Gly Asp Gly Glu Lys Ser Arg Lys Ala 20 25 30 gag atc aac ttt tct aaaaca aga aat ttg gcg aga aac aag cag aaa 144 Glu Ile Asn Phe Ser Lys ThrArg Asn Leu Ala Arg Asn Lys Gln Lys 35 40 45 cgc tgc agt agt tgg gca aagtat tgt gaa gtt gac tcg gaa tgc tgt 192 Arg Cys Ser Ser Trp Ala Lys TyrCys Glu Val Asp Ser Glu Cys Cys 50 55 60 tcc gaa cag tgt gta agg tct tactgc gcg atg tgg tgatgacctc 238 Ser Glu Gln Cys Val Arg Ser Tyr Cys AlaMet Trp 65 70 75 tgaccacaag ccatccgata tcaccactct cctcttcaga gacttcaag287 20 75 PRT Conus ammiralis 20 Met Gln Lys Leu Ile Ile Leu Leu Leu ValAla Ala Val Leu Met Ser 1 5 10 15 Thr Gln Ala Met Phe Gln Gly Asp GlyGlu Lys Ser Arg Lys Ala Glu 20 25 30 Ile Asn Phe Ser Lys Thr Arg Asn LeuAla Arg Asn Lys Gln Lys Arg 35 40 45 Cys Ser Ser Trp Ala Lys Tyr Cys GluVal Asp Ser Glu Cys Cys Ser 50 55 60 Glu Gln Cys Val Arg Ser Tyr Cys AlaMet Trp 65 70 75 21 27 PRT Conus ammiralis PEPTIDE (1)..(27) Xaa atresidues 9, 13 adn 17 is Glu or gamma-carboxy-Glu; Xaa at residue 13 isPro or hydroxy-Pro; Xaa at residues 4 and 27 is Trp (D or L) orbromo-Trp (D or L); 21 Cys Ser Ser Xaa Ala Lys Xaa Cys Xaa Val Asp SerXaa Cys Cys Ser 1 5 10 15 Xaa Gln Cys Val Arg Ser Xaa Cys Ala Met Xaa 2025 22 461 DNA Conus ammiralis CDS (4)..(270) 22 gtt aaa atg cat ctg tcactg gca cgc tca gct gtt ttg atg ttg ctt 48 Lys Met His Leu Ser Leu AlaArg Ser Ala Val Leu Met Leu Leu 1 5 10 15 ctg ctg ttt gcc ttg ggc aacttt gtt gtg gtc cag tca gga cag ata 96 Leu Leu Phe Ala Leu Gly Asn PheVal Val Val Gln Ser Gly Gln Ile 20 25 30 aca aga gat gtg gac aat gga cagctc acg gac aac cgc cgt aac ctg 144 Thr Arg Asp Val Asp Asn Gly Gln LeuThr Asp Asn Arg Arg Asn Leu 35 40 45 caa tcg aag tgg aag cca gtg agt ctcttc atg tca cga cgg tct tgt 192 Gln Ser Lys Trp Lys Pro Val Ser Leu PheMet Ser Arg Arg Ser Cys 50 55 60 aac aat tct tgc aat gag cat tcc gat tgcgaa tcc cat tgt att tgc 240 Asn Asn Ser Cys Asn Glu His Ser Asp Cys GluSer His Cys Ile Cys 65 70 75 acg ttt agc gga tgc aaa att att ttg atataaacggatt gagtttgctc 290 Thr Phe Ser Gly Cys Lys Ile Ile Leu Ile 80 85gtcaacaaga tgtcgcacta cagctcctct ctacagtgtg tacatcgacc aaacgacgca 350tcttttattt ctttgtctgt tgtatttgtt ttcctgtgtt cataacgtac agagcccttt 410aattaccttt actgctcttc acttaacctg ataaccggaa ggtccagtgc t 461 23 89 PRTConus ammiralis 23 Lys Met His Leu Ser Leu Ala Arg Ser Ala Val Leu MetLeu Leu Leu 1 5 10 15 Leu Phe Ala Leu Gly Asn Phe Val Val Val Gln SerGly Gln Ile Thr 20 25 30 Arg Asp Val Asp Asn Gly Gln Leu Thr Asp Asn ArgArg Asn Leu Gln 35 40 45 Ser Lys Trp Lys Pro Val Ser Leu Phe Met Ser ArgArg Ser Cys Asn 50 55 60 Asn Ser Cys Asn Glu His Ser Asp Cys Glu Ser HisCys Ile Cys Thr 65 70 75 80 Phe Ser Gly Cys Lys Ile Ile Leu Ile 85 24 28PRT Conus ammiralis PEPTIDE (1)..(28) Xaa at residues 8 and 13 is Glu orgamma-carboxy-Glu 24 Ser Cys Asn Asn Ser Cys Asn Xaa His Ser Asp Cys XaaSer His Cys 1 5 10 15 Ile Cys Thr Phe Ser Gly Cys Lys Ile Ile Leu Ile 2025 25 459 DNA Conus ammiralis CDS (7)..(270) 25 gttaaa atg cat ctg tcactg gca cgc tta gct gtt ttg atg ttg ctt 48 Met His Leu Ser Leu Ala ArgLeu Ala Val Leu Met Leu Leu 1 5 10 ctg ctg ttt gcc ttg ggc aac ttt gttgtg gtc cag tca gga cag ata 96 Leu Leu Phe Ala Leu Gly Asn Phe Val ValVal Gln Ser Gly Gln Ile 15 20 25 30 aca aga gat gtg gac aat gga cag ctcacg gac aac cgc cgt aac ctg 144 Thr Arg Asp Val Asp Asn Gly Gln Leu ThrAsp Asn Arg Arg Asn Leu 35 40 45 caa tcg aag tgg aag cca gtg agt ctc ttcatg tca cga cgg tct tgt 192 Gln Ser Lys Trp Lys Pro Val Ser Leu Phe MetSer Arg Arg Ser Cys 50 55 60 aac aat tct tgc aat gag cat tcc gat tgc gaatcc cat tgt att tgc 240 Asn Asn Ser Cys Asn Glu His Ser Asp Cys Glu SerHis Cys Ile Cys 65 70 75 acg ttt aga gga tgc gga gct gtt aat ggttgagtttgct cgtcaacatg 290 Thr Phe Arg Gly Cys Gly Ala Val Asn Gly 80 85atgtcgcact acacactaca gctcctctct acagtgtgta catcgaccaa acgacgcatc 350ttttatttct ttgtctgttg tgtttgtttt cctgtgttca taacgtacag agccctttaa 410ttacttttac tgctcttcac ttaacctgat aaccagaagg tccagtgct 459 26 88 PRTConus ammiralis 26 Met His Leu Ser Leu Ala Arg Leu Ala Val Leu Met LeuLeu Leu Leu 1 5 10 15 Phe Ala Leu Gly Asn Phe Val Val Val Gln Ser GlyGln Ile Thr Arg 20 25 30 Asp Val Asp Asn Gly Gln Leu Thr Asp Asn Arg ArgAsn Leu Gln Ser 35 40 45 Lys Trp Lys Pro Val Ser Leu Phe Met Ser Arg ArgSer Cys Asn Asn 50 55 60 Ser Cys Asn Glu His Ser Asp Cys Glu Ser His CysIle Cys Thr Phe 65 70 75 80 Arg Gly Cys Gly Ala Val Asn Gly 85 27 27 PRTConus ammiralis PEPTIDE (1)..(27) Xaa at residues 8 and 13 is Glu orgamma-carboxy-Glu 27 Ser Cys Asn Asn Ser Cys Asn Xaa His Ser Asp Cys XaaSer His Cys 1 5 10 15 Ile Cys Thr Phe Arg Gly Cys Gly Ala Val Asn 20 2528 387 DNA Conus arenatus CDS (19)..(240) 28 accaaaacca tcatcaaa atg aaactg acg tgc gtg gtg atc gtc gct gtg 51 Met Lys Leu Thr Cys Val Val IleVal Ala Val 1 5 10 ctg ttc ctg acg gcc tgt caa ctc act aca gct gat gactcc aga ggt 99 Leu Phe Leu Thr Ala Cys Gln Leu Thr Thr Ala Asp Asp SerArg Gly 15 20 25 acg cag aag cat ggt gcc ctg aga tcg acc acc aaa ctc tccatg ttg 147 Thr Gln Lys His Gly Ala Leu Arg Ser Thr Thr Lys Leu Ser MetLeu 30 35 40 act cgg ggc tgc acg cct cct ggt gga gtt tgt ggt tat cat ggtcac 195 Thr Arg Gly Cys Thr Pro Pro Gly Gly Val Cys Gly Tyr His Gly His45 50 55 tgc tgc gat ttt tgc gat acg ttc ggc aat tta tgt gtg agt ggc 240Cys Cys Asp Phe Cys Asp Thr Phe Gly Asn Leu Cys Val Ser Gly 60 65 70tgacccggca tctgaccttt cccccttctt tgctccacta tcctttttct gcctgagtcc 300tccatacctg agagctgtca tgaaccactc aacacctact cttccggagg tttctgagga 360gctgcattga aataaaagcc gcattgc 387 29 74 PRT Conus arenatus 29 Met LysLeu Thr Cys Val Val Ile Val Ala Val Leu Phe Leu Thr Ala 1 5 10 15 CysGln Leu Thr Thr Ala Asp Asp Ser Arg Gly Thr Gln Lys His Gly 20 25 30 AlaLeu Arg Ser Thr Thr Lys Leu Ser Met Leu Thr Arg Gly Cys Thr 35 40 45 ProPro Gly Gly Val Cys Gly Tyr His Gly His Cys Cys Asp Phe Cys 50 55 60 AspThr Phe Gly Asn Leu Cys Val Ser Gly 65 70 30 28 PRT Conus arenatusPEPTIDE (1)..(28) Xaa at residues 4 and 5 is Pro or hydroxy-Pro; Xaa atresidue 11 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 30 Gly Cys Thr Xaa Xaa Gly Gly Val Cys Gly Xaa His Gly HisCys Cys 1 5 10 15 Asp Phe Cys Asp Thr Phe Gly Asn Leu Cys Val Ser 20 2531 375 DNA Conus arenatus CDS (24)..(260) 31 gacaggattg aacaaaattc aggatg tca gga ttg gga atc atg gtg cta acc 53 Met Ser Gly Leu Gly Ile MetVal Leu Thr 1 5 10 ctt cta ctt ctt gtg ttc atg gca acc agt cat cag gatgca gga gag 101 Leu Leu Leu Leu Val Phe Met Ala Thr Ser His Gln Asp AlaGly Glu 15 20 25 aag aag gcg atg caa agg gac gca atc aac gtc aga cgg agaaga tca 149 Lys Lys Ala Met Gln Arg Asp Ala Ile Asn Val Arg Arg Arg ArgSer 30 35 40 ctc act cgg gga gta gta act gag gcg tgc gaa gag tcc tgt gaggag 197 Leu Thr Arg Gly Val Val Thr Glu Ala Cys Glu Glu Ser Cys Glu Glu45 50 55 gag gaa aag cac tgc tgc cac gta aat aat gga gta ccc tct tgt gcc245 Glu Glu Lys His Cys Cys His Val Asn Asn Gly Val Pro Ser Cys Ala 6065 70 gtt ata tgc tgg gga tagtttctcg cacactgtct cattcattat tttatcagta300 Val Ile Cys Trp Gly 75 caagtgtaaa cgagacatgt cagaaagtcg aaggttgtgcgtatttgata agtattgttt 360 actgggatga acgga 375 32 79 PRT Conus arenatus32 Met Ser Gly Leu Gly Ile Met Val Leu Thr Leu Leu Leu Leu Val Phe 1 510 15 Met Ala Thr Ser His Gln Asp Ala Gly Glu Lys Lys Ala Met Gln Arg 2025 30 Asp Ala Ile Asn Val Arg Arg Arg Arg Ser Leu Thr Arg Gly Val Val 3540 45 Thr Glu Ala Cys Glu Glu Ser Cys Glu Glu Glu Glu Lys His Cys Cys 5055 60 His Val Asn Asn Gly Val Pro Ser Cys Ala Val Ile Cys Trp Gly 65 7075 33 32 PRT Conus arenatus PEPTIDE (1)..(32) Xaa at residues 4, 7, 8,11, 12, 13 and 14 is Glu or gamma- carboxy-Glu; Xaa at residue 25 is Proor hydroxy-Pro; Xaa at residue 32 is Trp (D or L) or bromo-Trp (D or L)33 Val Val Thr Xaa Ala Cys Xaa Xaa Ser Cys Xaa Xaa Xaa Xaa Lys His 1 510 15 Cys Cys His Val Asn Asn Gly Val Xaa Ser Cys Ala Val Ile Cys Xaa 2025 30 34 375 DNA Conus arenatus CDS (24)..(260) 34 gacaggattg aacaaaattcagg atg tca gga ttg gga atc atg gtg cta acc 53 Met Ser Gly Leu Gly IleMet Val Leu Thr 1 5 10 ctt cta ctt ctt gtg ttc atg gca acc agt cat caggat gca gga gag 101 Leu Leu Leu Leu Val Phe Met Ala Thr Ser His Gln AspAla Gly Glu 15 20 25 aag cag gcg acg gaa agg gac gca atc aac atc aga tggaga aga tca 149 Lys Gln Ala Thr Glu Arg Asp Ala Ile Asn Ile Arg Trp ArgArg Ser 30 35 40 cgc act cgg aga ata gta act gag gcg tgc gaa gag tcc tgtgag gac 197 Arg Thr Arg Arg Ile Val Thr Glu Ala Cys Glu Glu Ser Cys GluAsp 45 50 55 gag gaa aag cac tgc tgc cac gta aat aat gga gta ccc tct tgtgcc 245 Glu Glu Lys His Cys Cys His Val Asn Asn Gly Val Pro Ser Cys Ala60 65 70 gtt ata tgc tgg gga tagtttctcg cacactgtct cattcattat tttatcagta300 Val Ile Cys Trp Gly 75 caagtgtaaa cgagacatgt cagaaagtcg aaggttgtgcgtatttgata agtattgttt 360 actgggatga acgga 375 35 79 PRT Conus arenatus35 Met Ser Gly Leu Gly Ile Met Val Leu Thr Leu Leu Leu Leu Val Phe 1 510 15 Met Ala Thr Ser His Gln Asp Ala Gly Glu Lys Gln Ala Thr Glu Arg 2025 30 Asp Ala Ile Asn Ile Arg Trp Arg Arg Ser Arg Thr Arg Arg Ile Val 3540 45 Thr Glu Ala Cys Glu Glu Ser Cys Glu Asp Glu Glu Lys His Cys Cys 5055 60 His Val Asn Asn Gly Val Pro Ser Cys Ala Val Ile Cys Trp Gly 65 7075 36 32 PRT Conus arenatus PEPTIDE (1)..(32) Xaa at residues 4, 7, 8,11, 13 adn 14 is Glu or gamma-carboxy- Glu; Xaa at residue 25 is Pro orhydroxy-Pro; Xaa at residue 32 is Trp (D or L) or bromo-Trp (D or L) 36Ile Val Thr Xaa Ala Cys Xaa Xaa Ser Cys Xaa Asp Xaa Xaa Lys His 1 5 1015 Cys Cys His Val Asn Asn Gly Val Xaa Ser Cys Ala Val Ile Cys Xaa 20 2530 37 374 DNA Conus arenatus CDS (24)..(260) 37 gacaggattg aacaaaattcagg atg tca gaa ctg gga atc atg gtg cta acg 53 Met Ser Glu Leu Gly IleMet Val Leu Thr 1 5 10 ctt cta ctt ctt gtg ttc ctg gta acc agt cat caggat gca gga gag 101 Leu Leu Leu Leu Val Phe Leu Val Thr Ser His Gln AspAla Gly Glu 15 20 25 aag cag gcg acg gaa agg gac gca atc aac atc aga tggaga aga tca 149 Lys Gln Ala Thr Glu Arg Asp Ala Ile Asn Ile Arg Trp ArgArg Ser 30 35 40 ctc act cgg aga ata gta act gag gcg tgc gaa gag cac tgtgag gat 197 Leu Thr Arg Arg Ile Val Thr Glu Ala Cys Glu Glu His Cys GluAsp 45 50 55 gag gaa cag ttc tgc tgc ggc tta gag aat gga caa ccc ttt tgtgcc 245 Glu Glu Gln Phe Cys Cys Gly Leu Glu Asn Gly Gln Pro Phe Cys Ala60 65 70 cct gtt tgc ttc gga tagtttctgt acactgtctc attaattatt ttatcagtac300 Pro Val Cys Phe Gly 75 aagtgtaaac aaaacatgtc agaaagtcga aggttgtgcgtatttgataa gtattgtttg 360 ctgggacgaa cgga 374 38 79 PRT Conus arenatus38 Met Ser Glu Leu Gly Ile Met Val Leu Thr Leu Leu Leu Leu Val Phe 1 510 15 Leu Val Thr Ser His Gln Asp Ala Gly Glu Lys Gln Ala Thr Glu Arg 2025 30 Asp Ala Ile Asn Ile Arg Trp Arg Arg Ser Leu Thr Arg Arg Ile Val 3540 45 Thr Glu Ala Cys Glu Glu His Cys Glu Asp Glu Glu Gln Phe Cys Cys 5055 60 Gly Leu Glu Asn Gly Gln Pro Phe Cys Ala Pro Val Cys Phe Gly 65 7075 39 32 PRT Conus arenatus PEPTIDE (1)..(32) Xaa at residues 4, 7, 8,11, 13, 14 and 21 is Glu or gamma- carboxy-Glu; Xaa at residues 25 and29 is Pro or hydroxy-Pro 39 Ile Val Thr Xaa Ala Cys Xaa Xaa His Cys XaaAsp Xaa Xaa Gln Phe 1 5 10 15 Cys Cys Gly Leu Xaa Asn Gly Gln Xaa PheCys Ala Xaa Val Cys Phe 20 25 30 40 375 DNA Conus arenatus CDS(24)..(260) 40 gacaggattg aacaaaattc agg atg tca gga ttg gga atc atg gtgcta acc 53 Met Ser Gly Leu Gly Ile Met Val Leu Thr 1 5 10 ctt cta cttctt gtg ttc atg gca acc agt cat cag gat gca gga gag 101 Leu Leu Leu LeuVal Phe Met Ala Thr Ser His Gln Asp Ala Gly Glu 15 20 25 aag aag gtg atgcaa agg gac gca atc aac gtc aga cgg aga aga tca 149 Lys Lys Val Met GlnArg Asp Ala Ile Asn Val Arg Arg Arg Arg Ser 30 35 40 cgc act cgg aga gtagta act ggg gcg tgc gaa gag cac tgt gag gac 197 Arg Thr Arg Arg Val ValThr Gly Ala Cys Glu Glu His Cys Glu Asp 45 50 55 gag gaa aag cac tgc tgcggc tta gag aat gga caa ccc ttt tgt gcc 245 Glu Glu Lys His Cys Cys GlyLeu Glu Asn Gly Gln Pro Phe Cys Ala 60 65 70 cgt cta tgc tta ggatagttttctg tacactgtct tattcattat tttatcagta 300 Arg Leu Cys Leu Gly 75caagtgaaaa caaagcatgt cagaaagtcg aaggttgtgc gtatttgata agtattgttt 360actgggatga acgga 375 41 79 PRT Conus arenatus 41 Met Ser Gly Leu Gly IleMet Val Leu Thr Leu Leu Leu Leu Val Phe 1 5 10 15 Met Ala Thr Ser HisGln Asp Ala Gly Glu Lys Lys Val Met Gln Arg 20 25 30 Asp Ala Ile Asn ValArg Arg Arg Arg Ser Arg Thr Arg Arg Val Val 35 40 45 Thr Gly Ala Cys GluGlu His Cys Glu Asp Glu Glu Lys His Cys Cys 50 55 60 Gly Leu Glu Asn GlyGln Pro Phe Cys Ala Arg Leu Cys Leu Gly 65 70 75 42 32 PRT Conusarenatus PEPTIDE (1)..(31) Xaa at residues 7, 8, 11, 13, 14 and 21 isGlu or gamma-carboxy- Glu; Xaa at residue 25 is Pro or hydroxy-Pro 42Val Val Thr Gly Ala Cys Xaa Xaa His Cys Xaa Asp Xaa Xaa Lys His 1 5 1015 Cys Cys Gly Leu Xaa Asn Gly Gln Xaa Phe Cys Ala Arg Leu Cys Leu 20 2530 43 219 DNA Conus arenatus CDS (1)..(189) 43 atg ggg aaa ctg aca atactg gtt ctt gtt gct gct gta ctg ttg tcg 48 Met Gly Lys Leu Thr Ile LeuVal Leu Val Ala Ala Val Leu Leu Ser 1 5 10 15 acc cag gtc atg gtt caaggt gac gga gat caa cct gca gct cgc aat 96 Thr Gln Val Met Val Gln GlyAsp Gly Asp Gln Pro Ala Ala Arg Asn 20 25 30 gca gtg cca aaa gac gat aaccca gat gga gcg agt gga aag ttc atg 144 Ala Val Pro Lys Asp Asp Asn ProAsp Gly Ala Ser Gly Lys Phe Met 35 40 45 aat gtt cta cgt cgg tct gga tgtccg tgg cat cct tgg tgt ggc 189 Asn Val Leu Arg Arg Ser Gly Cys Pro TrpHis Pro Trp Cys Gly 50 55 60 tgatcggaat ccacgattgc aatgacagcc 219 44 63PRT Conus arenatus 44 Met Gly Lys Leu Thr Ile Leu Val Leu Val Ala AlaVal Leu Leu Ser 1 5 10 15 Thr Gln Val Met Val Gln Gly Asp Gly Asp GlnPro Ala Ala Arg Asn 20 25 30 Ala Val Pro Lys Asp Asp Asn Pro Asp Gly AlaSer Gly Lys Phe Met 35 40 45 Asn Val Leu Arg Arg Ser Gly Cys Pro Trp HisPro Trp Cys Gly 50 55 60 45 9 PRT Conus arenatus PEPTIDE (1)..(9) Xaa atresidues 4 and 7 is Pro or hydroxy-Pro; Xaa at residues 5 and 8 is Trp(D or L) or bromo-Trp (D or L) 45 Ser Gly Cys Xaa Xaa His Xaa Xaa Cys 15 46 219 DNA Conus arenatus CDS (1)..(189) 46 atg ggg aaa ctg aca atactg gtt ctt gtt gct gct gta ctg ttg tcg 48 Met Gly Lys Leu Thr Ile LeuVal Leu Val Ala Ala Val Leu Leu Ser 1 5 10 15 acc cag gtc atg gtt caaggt gac gga gat caa cct gca gct cgc aat 96 Thr Gln Val Met Val Gln GlyAsp Gly Asp Gln Pro Ala Ala Arg Asn 20 25 30 gca gtg cca aaa gac gat aaccca gat gga gcg agt gga aag ttc atg 144 Ala Val Pro Lys Asp Asp Asn ProAsp Gly Ala Ser Gly Lys Phe Met 35 40 45 aat gtt cta cgt cgg tct gga tgtccg tgg cgc cct tgg tgt ggc 189 Asn Val Leu Arg Arg Ser Gly Cys Pro TrpArg Pro Trp Cys Gly 50 55 60 tgatcggaat ccacgattgc aatgacagcc 219 47 63PRT Conus arenatus 47 Met Gly Lys Leu Thr Ile Leu Val Leu Val Ala AlaVal Leu Leu Ser 1 5 10 15 Thr Gln Val Met Val Gln Gly Asp Gly Asp GlnPro Ala Ala Arg Asn 20 25 30 Ala Val Pro Lys Asp Asp Asn Pro Asp Gly AlaSer Gly Lys Phe Met 35 40 45 Asn Val Leu Arg Arg Ser Gly Cys Pro Trp ArgPro Trp Cys Gly 50 55 60 48 10 PRT Conus arenatus PEPTIDE (1)..(10) Xaaat residues 5 and 8 is Pro or hydroxy-Pro; Xaa at residues 6 and 9 isTrp (D or L) or bromo-Trp (D or L) 48 Ala Ser Gly Cys Xaa Xaa Arg XaaXaa Cys 1 5 10 49 219 DNA Conus arenatus CDS (1)..(189) 49 atg ggg aaactg aca ata ctg gtt ctt gtt gct gct gta ctg ttg tcg 48 Met Gly Lys LeuThr Ile Leu Val Leu Val Ala Ala Val Leu Leu Ser 1 5 10 15 acc cag gtcatg gtt caa ggt gac gga gat caa cct gca ggt cga gat 96 Thr Gln Val MetVal Gln Gly Asp Gly Asp Gln Pro Ala Gly Arg Asp 20 25 30 gca gtt cca agagac gat aac cca ggt gga acg agt gga aag ttc atg 144 Ala Val Pro Arg AspAsp Asn Pro Gly Gly Thr Ser Gly Lys Phe Met 35 40 45 aat gct cta cgt caatat gga tgt ccg gtg ggt ctt tgg tgt gac 189 Asn Ala Leu Arg Gln Tyr GlyCys Pro Val Gly Leu Trp Cys Asp 50 55 60 tgatcagaat ccacgattgcaatgacagcc 219 50 63 PRT Conus arenatus 50 Met Gly Lys Leu Thr Ile LeuVal Leu Val Ala Ala Val Leu Leu Ser 1 5 10 15 Thr Gln Val Met Val GlnGly Asp Gly Asp Gln Pro Ala Gly Arg Asp 20 25 30 Ala Val Pro Arg Asp AspAsn Pro Gly Gly Thr Ser Gly Lys Phe Met 35 40 45 Asn Ala Leu Arg Gln TyrGly Cys Pro Val Gly Leu Trp Cys Asp 50 55 60 51 11 PRT Conus arenatusPEPTIDE (1)..(11) Xaa at residue 1 is Gln or pyro-Gln; Xaa at residue 5is Pro or hydroxy-Pro; Xaa at residue 9 is Trp (D or L) or bromo-Trp Dor L); Xaa at residue 2 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr,O-sulpho-Tyr or O-phospho-Tyr 51 Xaa Xaa Gly Cys Xaa Val Gly Leu Xaa CysAsp 1 5 10 52 210 DNA Conus arenatus CDS (1)..(180) 52 atg ggg aaa ctgaca ata ctg gtt ctt gtt gct gct gta ctg ttg tcg 48 Met Gly Lys Leu ThrIle Leu Val Leu Val Ala Ala Val Leu Leu Ser 1 5 10 15 acc cag gtc atgttt cga gat caa cct gca cgt cgt gat gca gtg cca 96 Thr Gln Val Met PheArg Asp Gln Pro Ala Arg Arg Asp Ala Val Pro 20 25 30 aga gac gat agc ccagat gga atg agt gga ggg ttc atg aat gtc cca 144 Arg Asp Asp Ser Pro AspGly Met Ser Gly Gly Phe Met Asn Val Pro 35 40 45 cgt cgg tct gga tgt ccgtgg caa cct tgg tgt ggc tgatcggaat 190 Arg Arg Ser Gly Cys Pro Trp GlnPro Trp Cys Gly 50 55 60 ccacgattgc aatgacagcc 210 53 60 PRT Conusarenatus 53 Met Gly Lys Leu Thr Ile Leu Val Leu Val Ala Ala Val Leu LeuSer 1 5 10 15 Thr Gln Val Met Phe Arg Asp Gln Pro Ala Arg Arg Asp AlaVal Pro 20 25 30 Arg Asp Asp Ser Pro Asp Gly Met Ser Gly Gly Phe Met AsnVal Pro 35 40 45 Arg Arg Ser Gly Cys Pro Trp Gln Pro Trp Cys Gly 50 5560 54 9 PRT Conus arenatus PEPTIDE (1)..(9) Xaa at residues 4 and 7 isPro or hydroxy-Pro; Xaa at residues 5 and 8 is Trp (D or L) or bromo-Trp(D or L) 54 Ser Gly Cys Xaa Xaa Gln Xaa Xaa Cys 1 5 55 210 DNA Conusarenatus CDS (1)..(180) 55 atg ggg aaa ctg aca ata ctg gtt ctt gtt gctgct gta ctg ttg tcg 48 Met Gly Lys Leu Thr Ile Leu Val Leu Val Ala AlaVal Leu Leu Ser 1 5 10 15 acc cag gcc atg gtt caa gat caa cct gca ggtcga gat gca gtt cca 96 Thr Gln Ala Met Val Gln Asp Gln Pro Ala Gly ArgAsp Ala Val Pro 20 25 30 aga gac gat aac cca ggt gga acg agt gga aag ttcgtg aat gct caa 144 Arg Asp Asp Asn Pro Gly Gly Thr Ser Gly Lys Phe ValAsn Ala Gln 35 40 45 cgt caa tat gga tgt ccg ccg ggt ctt tgg tgt cactgatcagaat 190 Arg Gln Tyr Gly Cys Pro Pro Gly Leu Trp Cys His 50 55 60ccacgattgc aatgacagcc 210 56 60 PRT Conus arenatus 56 Met Gly Lys LeuThr Ile Leu Val Leu Val Ala Ala Val Leu Leu Ser 1 5 10 15 Thr Gln AlaMet Val Gln Asp Gln Pro Ala Gly Arg Asp Ala Val Pro 20 25 30 Arg Asp AspAsn Pro Gly Gly Thr Ser Gly Lys Phe Val Asn Ala Gln 35 40 45 Arg Gln TyrGly Cys Pro Pro Gly Leu Trp Cys His 50 55 60 57 11 PRT Conus arenatusPEPTIDE (1)..(11) Xaa at residue 1 is Gln or pyro-Gln;Xaa at residues 5and 6 is Pro or hydroxy-Pro; Xaa at residue 9 is Trp (D or L) orbromo-Trp (D or L); Xaa at residue 2 is Tyr, 125I-Tyr, mono-iodo-Tyr,di- iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 57 Xaa Xaa Gly Cys Xaa XaaGly Leu Xaa Cys His 1 5 10 58 213 DNA Conus aurisiacus CDS (1)..(189) 58atg ttc acc gtg ttt ctg ttg gtt gtc ttg gca acc act gtc gtt tcc 48 MetPhe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val Val Ser 1 5 10 15atc cct tca gat cgt gca tct gat ggc agg aat gcc gca gtc aac gag 96 IlePro Ser Asp Arg Ala Ser Asp Gly Arg Asn Ala Ala Val Asn Glu 20 25 30 agagcg cct tgg ctg gtc cct tcg aca atc acg act tgc tgt gga tat 144 Arg AlaPro Trp Leu Val Pro Ser Thr Ile Thr Thr Cys Cys Gly Tyr 35 40 45 aat ccgggg aca atg tgc cct cct tgc agg tgc gat aat acc tgt 189 Asn Pro Gly ThrMet Cys Pro Pro Cys Arg Cys Asp Asn Thr Cys 50 55 60 taaccaaaaaaaaaaaaaaa aaaa 213 59 63 PRT Conus aurisiacus 59 Met Phe Thr Val PheLeu Leu Val Val Leu Ala Thr Thr Val Val Ser 1 5 10 15 Ile Pro Ser AspArg Ala Ser Asp Gly Arg Asn Ala Ala Val Asn Glu 20 25 30 Arg Ala Pro TrpLeu Val Pro Ser Thr Ile Thr Thr Cys Cys Gly Tyr 35 40 45 Asn Pro Gly ThrMet Cys Pro Pro Cys Arg Cys Asp Asn Thr Cys 50 55 60 60 30 PRT Conusaurisiacus PEPTIDE (1)..(30) Xaa at residues 2, 6, 17, 22 and 23 is Proor hydroxy-Pro; Xaa at residue 3 is Trp (D or L) or bromo-Trp (D or L);Xaa at residue 15 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr,O-sulpho-Tyr or O-phospho-Tyr 60 Ala Xaa Xaa Leu Val Xaa Ser Thr Ile ThrThr Cys Cys Gly Xaa Asn 1 5 10 15 Xaa Gly Thr Met Cys Xaa Xaa Cys ArgCys Asp Asn Thr Cys 20 25 30 61 202 DNA Conus bandanus CDS (1)..(183) 61atg cgc tgt ctc cca gtc ttg atc att ctt ctg ctg ctg act gca tct 48 MetArg Cys Leu Pro Val Leu Ile Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15gca cct ggc gtt gat gtc cta ccg aag acc gaa gat gat gtg ccc ctg 96 AlaPro Gly Val Asp Val Leu Pro Lys Thr Glu Asp Asp Val Pro Leu 20 25 30 tcatct gtc tac gat aat aca aag agt atc cta cga gga ctt ctg gac 144 Ser SerVal Tyr Asp Asn Thr Lys Ser Ile Leu Arg Gly Leu Leu Asp 35 40 45 aaa cgtgct tgc tgt ggc tac aag ctt tgc tca cca tgt taaccagcat 193 Lys Arg AlaCys Cys Gly Tyr Lys Leu Cys Ser Pro Cys 50 55 60 gaaggatcc 202 62 61 PRTConus bandanus 62 Met Arg Cys Leu Pro Val Leu Ile Ile Leu Leu Leu LeuThr Ala Ser 1 5 10 15 Ala Pro Gly Val Asp Val Leu Pro Lys Thr Glu AspAsp Val Pro Leu 20 25 30 Ser Ser Val Tyr Asp Asn Thr Lys Ser Ile Leu ArgGly Leu Leu Asp 35 40 45 Lys Arg Ala Cys Cys Gly Tyr Lys Leu Cys Ser ProCys 50 55 60 63 11 PRT Conus bandanus PEPTIDE (1)..(11) Xaa at residue10 is Pro or hydroxy-Pro; Xaa at residue 5 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho- Tyr 63 Ala CysCys Gly Xaa Lys Leu Cys Ser Xaa Cys 1 5 10 64 359 DNA Conuscaracteristicus CDS (7)..(246) 64 ggatcc atg aaa ctg acg tgc gtg gtg atcatc gcc gcg ctg ttc ctg 48 Met Lys Leu Thr Cys Val Val Ile Ile Ala AlaLeu Phe Leu 1 5 10 acg gcc tgt cag ctc aat aca gct gat gac tcc aga gataag cag gag 96 Thr Ala Cys Gln Leu Asn Thr Ala Asp Asp Ser Arg Asp LysGln Glu 15 20 25 30 tac cgt gca gtg agg ttg aga gac gga atg cgg aat ttcaaa ggt tcc 144 Tyr Arg Ala Val Arg Leu Arg Asp Gly Met Arg Asn Phe LysGly Ser 35 40 45 aag cgc aac tgc ggg gaa caa ggt gaa ggt tgt gct act cgccca tgc 192 Lys Arg Asn Cys Gly Glu Gln Gly Glu Gly Cys Ala Thr Arg ProCys 50 55 60 tgc tct ggt ctg agt tgc gtt ggc agc cgt cca gga ggc ctg tgccag 240 Cys Ser Gly Leu Ser Cys Val Gly Ser Arg Pro Gly Gly Leu Cys Gln65 70 75 tac ggc taatagtctg gcatctgata tttcccctct gcactctacc ttcttttgcc296 Tyr Gly 80 tgatgcatgt ttacttgtgt gtggtcatga accactcagt agctacacctccgaaggacg 356 tgc 359 65 80 PRT Conus caracteristicus 65 Met Lys LeuThr Cys Val Val Ile Ile Ala Ala Leu Phe Leu Thr Ala 1 5 10 15 Cys GlnLeu Asn Thr Ala Asp Asp Ser Arg Asp Lys Gln Glu Tyr Arg 20 25 30 Ala ValArg Leu Arg Asp Gly Met Arg Asn Phe Lys Gly Ser Lys Arg 35 40 45 Asn CysGly Glu Gln Gly Glu Gly Cys Ala Thr Arg Pro Cys Cys Ser 50 55 60 Gly LeuSer Cys Val Gly Ser Arg Pro Gly Gly Leu Cys Gln Tyr Gly 65 70 75 80 6631 PRT Conus caracteristicus PEPTIDE (1)..(31) Xaa at residues 4 and 7is Glu or gamma-carboxy-Glu; Xaa at residues 13 and 25 is Pro orhydroxy-Pro; Xaa at residue 31 is Tyr, Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 66 Asn Cys Gly Xaa Gln GlyXaa Gly Cys Ala Thr Arg Xaa Cys Cys Ser 1 5 10 15 Gly Leu Ser Cys ValGly Ser Arg Xaa Gly Gly Leu Cys Gln Xaa 20 25 30 67 349 DNA Conuscaracteristicus CDS (1)..(270) 67 atg atg tcg aaa atg gga gct atg tttgtc ctt ttg ctt ctt ttc atc 48 Met Met Ser Lys Met Gly Ala Met Phe ValLeu Leu Leu Leu Phe Ile 1 5 10 15 ctg cca tcc agc cag cag gaa gga gatgtc cag gca aga aaa acg cac 96 Leu Pro Ser Ser Gln Gln Glu Gly Asp ValGln Ala Arg Lys Thr His 20 25 30 ctg aag aga ggc ttc tac ggt act ctg gcaatg tct acc aga gga tgc 144 Leu Lys Arg Gly Phe Tyr Gly Thr Leu Ala MetSer Thr Arg Gly Cys 35 40 45 tct ggc act tgc cat cgt cgt gag gac ggc aagtgt cgg ggt act tgc 192 Ser Gly Thr Cys His Arg Arg Glu Asp Gly Lys CysArg Gly Thr Cys 50 55 60 gac tgc tcc gga tac agc tat tgt cgc tgc ggt gacgct cac cat ttt 240 Asp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly Asp AlaHis His Phe 65 70 75 80 tac cga gga tgc acg tgt tcg tgt caa ggttgattaattg actcttttaa 290 Tyr Arg Gly Cys Thr Cys Ser Cys Gln Gly 85 90ctcgttgaac gattgaaaaa aaaaatttta gagcaatatg ttcgagaaaa accgaagac 349 6890 PRT Conus caracteristicus 68 Met Met Ser Lys Met Gly Ala Met Phe ValLeu Leu Leu Leu Phe Ile 1 5 10 15 Leu Pro Ser Ser Gln Gln Glu Gly AspVal Gln Ala Arg Lys Thr His 20 25 30 Leu Lys Arg Gly Phe Tyr Gly Thr LeuAla Met Ser Thr Arg Gly Cys 35 40 45 Ser Gly Thr Cys His Arg Arg Glu AspGly Lys Cys Arg Gly Thr Cys 50 55 60 Asp Cys Ser Gly Tyr Ser Tyr Cys ArgCys Gly Asp Ala His His Phe 65 70 75 80 Tyr Arg Gly Cys Thr Cys Ser CysGln Gly 85 90 69 43 PRT Conus caracteristicus PEPTIDE (1)..(43) Xaa atresidue 10 is Glu or gamma-carboxy-Glu; Xaa at residues 23, 25 and 35 isTyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr69 Gly Cys Ser Gly Thr Cys His Arg Arg Xaa Asp Gly Lys Cys Arg Gly 1 510 15 Thr Cys Asp Cys Ser Gly Xaa Ser Xaa Cys Arg Cys Gly Asp Ala His 2025 30 His Phe Xaa Arg Gly Cys Thr Cys Ser Cys Gln 35 40 70 346 DNA Conuscaracteristicus CDS (1)..(264) 70 atg atg tcg aaa atg gga gct atg tttgtc ctt ttg ctt ctt ttc atc 48 Met Met Ser Lys Met Gly Ala Met Phe ValLeu Leu Leu Leu Phe Ile 1 5 10 15 ctg cca tcc agc cag cag gaa gga gatgtc cag gca aga aaa acg cac 96 Leu Pro Ser Ser Gln Gln Glu Gly Asp ValGln Ala Arg Lys Thr His 20 25 30 cgg aag agc ggc ttc tac ggt act ctg gcaatg tct gcc aga gga tgc 144 Arg Lys Ser Gly Phe Tyr Gly Thr Leu Ala MetSer Ala Arg Gly Cys 35 40 45 tct ggc act tgc cat cgt cgt gag gac ggc aagtgt cgg ggt act tgc 192 Ser Gly Thr Cys His Arg Arg Glu Asp Gly Lys CysArg Gly Thr Cys 50 55 60 gac tgc tcc gga tac agc tat tgt cgc tgc ggt gacgct cac cat ttt 240 Asp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly Asp AlaHis His Phe 65 70 75 80 tac cga gga tgc acg tgt aca tgt taaggttgattaattgactc ttttaactcg 294 Tyr Arg Gly Cys Thr Cys Thr Cys 85 ttgaaccgattaaaaaaaaa attagacgaa tatgttcgag aaaaccgaag ac 346 71 88 PRT Conuscaracteristicus 71 Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu LeuLeu Phe Ile 1 5 10 15 Leu Pro Ser Ser Gln Gln Glu Gly Asp Val Gln AlaArg Lys Thr His 20 25 30 Arg Lys Ser Gly Phe Tyr Gly Thr Leu Ala Met SerAla Arg Gly Cys 35 40 45 Ser Gly Thr Cys His Arg Arg Glu Asp Gly Lys CysArg Gly Thr Cys 50 55 60 Asp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly AspAla His His Phe 65 70 75 80 Tyr Arg Gly Cys Thr Cys Thr Cys 85 72 42 PRTConus caracteristicus PEPTIDE (1)..(42) Xaa at residue 10 is Glu orgamma-carboxy-Glu; Xaa at residues 23, 25 and 35 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 72 Gly Cys SerGly Thr Cys His Arg Arg Xaa Asp Gly Lys Cys Arg Gly 1 5 10 15 Thr CysAsp Cys Ser Gly Xaa Ser Xaa Cys Arg Cys Gly Asp Ala His 20 25 30 His PheXaa Arg Gly Cys Thr Cys Thr Cys 35 40 73 348 DNA Conus caracteristicusCDS (1)..(264) 73 atg atg tcg aaa atg gga gct atg ttt gtc ctt ttg cttctt ttc atc 48 Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu Leu LeuPhe Ile 1 5 10 15 ctg cca tcc agc cag cag gaa gga gat gtc cag gca agaaaa acg cac 96 Leu Pro Ser Ser Gln Gln Glu Gly Asp Val Gln Ala Arg LysThr His 20 25 30 cgg aag agc ggc ttc tac ggt act ctg gca atg tct acc agagga tgc 144 Arg Lys Ser Gly Phe Tyr Gly Thr Leu Ala Met Ser Thr Arg GlyCys 35 40 45 tct ggc act tgc cgt cgt cat cgg gac ggc aag tgt cgg ggt acttgc 192 Ser Gly Thr Cys Arg Arg His Arg Asp Gly Lys Cys Arg Gly Thr Cys50 55 60 gac tgc tcc gga tac agc tat tgt cgc tgc ggt gac gct cac cat ttt240 Asp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly Asp Ala His His Phe 6570 75 80 tac cga gga tgc acg tgt aca tgt taaggttgat taattcgatcttttaactcg 294 Tyr Arg Gly Cys Thr Cys Thr Cys 85 ttgaacgatt aaaaaaaaaattttagacga atatgttcga gaaaaaccga agac 348 74 88 PRT Conuscaracteristicus 74 Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu LeuLeu Phe Ile 1 5 10 15 Leu Pro Ser Ser Gln Gln Glu Gly Asp Val Gln AlaArg Lys Thr His 20 25 30 Arg Lys Ser Gly Phe Tyr Gly Thr Leu Ala Met SerThr Arg Gly Cys 35 40 45 Ser Gly Thr Cys Arg Arg His Arg Asp Gly Lys CysArg Gly Thr Cys 50 55 60 Asp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly AspAla His His Phe 65 70 75 80 Tyr Arg Gly Cys Thr Cys Thr Cys 85 75 42 PRTConus caracteristicus PEPTIDE (1)..(42) Xaa at residues 23, 25 and 35 isTyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr75 Gly Cys Ser Gly Thr Cys Arg Arg His Arg Asp Gly Lys Cys Arg Gly 1 510 15 Thr Cys Asp Cys Ser Gly Xaa Ser Xaa Cys Arg Cys Gly Asp Ala His 2025 30 His Phe Xaa Arg Gly Cys Thr Cys Thr Cys 35 40 76 349 DNA Conuscaracteristicus CDS (1)..(264) 76 atg atg tcg aaa atg gga gct atg tttgtc ctt ttg ctt ctt ttc atc 48 Met Met Ser Lys Met Gly Ala Met Phe ValLeu Leu Leu Leu Phe Ile 1 5 10 15 ctg cca tcc agc cag cag gaa gga gatgtc cag gca aga aaa acg cac 96 Leu Pro Ser Ser Gln Gln Glu Gly Asp ValGln Ala Arg Lys Thr His 20 25 30 ctg aag aga ggc ttc tac ggt act ctg gcaatg tct acc aga gga tgc 144 Leu Lys Arg Gly Phe Tyr Gly Thr Leu Ala MetSer Thr Arg Gly Cys 35 40 45 tct ggc act tgc cgt cgt cat cgg gac ggc aagtgt cgg ggt act tgc 192 Ser Gly Thr Cys Arg Arg His Arg Asp Gly Lys CysArg Gly Thr Cys 50 55 60 gac tgc tcc gga tac agc tat tgt cgc tgc ggt gacgct cac cat ttt 240 Asp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly Asp AlaHis His Phe 65 70 75 80 tac cga gga tgc acg tgt aca tgt taaggttgattaattgactc ttttaactcg 294 Tyr Arg Gly Cys Thr Cys Thr Cys 85 ttgaacgattaaaaaaaaaa attttagagc aatatgttcg agaaaaaccg aagac 349 77 88 PRT Conuscaracteristicus 77 Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu LeuLeu Phe Ile 1 5 10 15 Leu Pro Ser Ser Gln Gln Glu Gly Asp Val Gln AlaArg Lys Thr His 20 25 30 Leu Lys Arg Gly Phe Tyr Gly Thr Leu Ala Met SerThr Arg Gly Cys 35 40 45 Ser Gly Thr Cys Arg Arg His Arg Asp Gly Lys CysArg Gly Thr Cys 50 55 60 Asp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly AspAla His His Phe 65 70 75 80 Tyr Arg Gly Cys Thr Cys Thr Cys 85 78 42 PRTConus caracteristicus PEPTIDE (1)..(42) Xaa at residues 23, 25 and 35 isTyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr78 Gly Cys Ser Gly Thr Cys Arg Arg His Arg Asp Gly Lys Cys Arg Gly 1 510 15 Thr Cys Asp Cys Ser Gly Xaa Ser Xaa Cys Arg Cys Gly Asp Ala His 2025 30 His Phe Xaa Arg Gly Cys Thr Cys Thr Cys 35 40 79 345 DNA Conuscaracteristicus CDS (1)..(264) 79 atg atg tcg aaa atg gga gct atg tttgtc ctt ttg ttt ctt ttc acc 48 Met Met Ser Lys Met Gly Ala Met Phe ValLeu Leu Phe Leu Phe Thr 1 5 10 15 ctg cca tcc agc cag cag gaa gga gatgtc cag gca aga aaa acg cac 96 Leu Pro Ser Ser Gln Gln Glu Gly Asp ValGln Ala Arg Lys Thr His 20 25 30 ctg aag aga ggc ttc tac ggt act ctg gcaatg tct tcc aga gga tgc 144 Leu Lys Arg Gly Phe Tyr Gly Thr Leu Ala MetSer Ser Arg Gly Cys 35 40 45 tct ggc act tgc cat cgt cgt gag gac ggc aagtgt cgg ggt act tgc 192 Ser Gly Thr Cys His Arg Arg Glu Asp Gly Lys CysArg Gly Thr Cys 50 55 60 gac tgc tcc gga tac agc tat tgt cgc tgc ggt gacgct cac cat ttt 240 Asp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly Asp AlaHis His Phe 65 70 75 80 tac cga gga tgt acg tgt aca tgt taaggttgattaattgactc ttttaactcg 294 Tyr Arg Gly Cys Thr Cys Thr Cys 85 ttgaacgattaaaaaaaaat ttagagcaat atgttcgaga aaaccgaaga c 345 80 88 PRT Conuscaracteristicus 80 Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu PheLeu Phe Thr 1 5 10 15 Leu Pro Ser Ser Gln Gln Glu Gly Asp Val Gln AlaArg Lys Thr His 20 25 30 Leu Lys Arg Gly Phe Tyr Gly Thr Leu Ala Met SerSer Arg Gly Cys 35 40 45 Ser Gly Thr Cys His Arg Arg Glu Asp Gly Lys CysArg Gly Thr Cys 50 55 60 Asp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly AspAla His His Phe 65 70 75 80 Tyr Arg Gly Cys Thr Cys Thr Cys 85 81 42 PRTConus caracteristicus PEPTIDE (1)..(42) Xaa at residue 10 is Glu orgamma-carboxy-Glu; Xaa at residues 23, 25 and 35 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 81 Gly Cys SerGly Thr Cys His Arg Arg Xaa Asp Gly Lys Cys Arg Gly 1 5 10 15 Thr CysAsp Cys Ser Gly Xaa Ser Xaa Cys Arg Cys Gly Asp Ala His 20 25 30 His PheXaa Arg Gly Cys Thr Cys Thr Cys 35 40 82 344 DNA Conus caracteristicusCDS (1)..(264) 82 atg atg tcg aaa atg gga gct atg ttt gtc ctt ttg cttctt ttc atc 48 Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu Leu LeuPhe Ile 1 5 10 15 ctg cca tcc agc cag cag gaa gga gat gtc cag gca agaaaa acg cac 96 Leu Pro Ser Ser Gln Gln Glu Gly Asp Val Gln Ala Arg LysThr His 20 25 30 ctg aag agc ggc ttc tac ggt act ctg gca atg tct gcc agagga tgc 144 Leu Lys Ser Gly Phe Tyr Gly Thr Leu Ala Met Ser Ala Arg GlyCys 35 40 45 tct ggc act tgc cat cgt cgt caa aac ggc gag tgt cag ggt acttgc 192 Ser Gly Thr Cys His Arg Arg Gln Asn Gly Glu Cys Gln Gly Thr Cys50 55 60 gac tgc gac gga cac gac cat tgt gac tgc ggt gac act ctc ggt act240 Asp Cys Asp Gly His Asp His Cys Asp Cys Gly Asp Thr Leu Gly Thr 6570 75 80 tac tca gga tgc gtg tgt ata tgt taaggttgat taattgactcttttaactcg 294 Tyr Ser Gly Cys Val Cys Ile Cys 85 ttgaacgatt aaaaaaatttagagcaatat gttcgagaaa aaccgaagac 344 83 88 PRT Conus caracteristicus 83Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu Leu Leu Phe Ile 1 5 1015 Leu Pro Ser Ser Gln Gln Glu Gly Asp Val Gln Ala Arg Lys Thr His 20 2530 Leu Lys Ser Gly Phe Tyr Gly Thr Leu Ala Met Ser Ala Arg Gly Cys 35 4045 Ser Gly Thr Cys His Arg Arg Gln Asn Gly Glu Cys Gln Gly Thr Cys 50 5560 Asp Cys Asp Gly His Asp His Cys Asp Cys Gly Asp Thr Leu Gly Thr 65 7075 80 Tyr Ser Gly Cys Val Cys Ile Cys 85 84 42 PRT Conus caracteristicusPEPTIDE (1)..(42) Xaa at residue 13 is Glu or gamma-carboxy-Glu; Xaa atresidue 35 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 84 Gly Cys Ser Gly Thr Cys His Arg Arg Gln Asn Gly Xaa CysGln Gly 1 5 10 15 Thr Cys Asp Cys Asp Gly His Asp His Cys Asp Cys GlyAsp Thr Leu 20 25 30 Gly Thr Xaa Ser Gly Cys Val Cys Ile Cys 35 40 85422 DNA Conus caracteristicus CDS (7)..(258) 85 gttaca atg cat ctg tcactg gca cgc tca gct gtc ttg atg ttg ctt 48 Met His Leu Ser Leu Ala ArgSer Ala Val Leu Met Leu Leu 1 5 10 ctg ctg ttt gcc ttg gac aac ttc gttggg gtc cag cca gga cag ata 96 Leu Leu Phe Ala Leu Asp Asn Phe Val GlyVal Gln Pro Gly Gln Ile 15 20 25 30 aca aga gat gtg gac aac cgc cgt aaccgg caa tcg cga tgg aag cca 144 Thr Arg Asp Val Asp Asn Arg Arg Asn ArgGln Ser Arg Trp Lys Pro 35 40 45 agg agt ctc ttc aag tca ctt cat aaa cgagca tcg tgt gga ggg act 192 Arg Ser Leu Phe Lys Ser Leu His Lys Arg AlaSer Cys Gly Gly Thr 50 55 60 tgc acg gaa agt gcc gat tgc cct tcc acg tgtagt act tgc tta cat 240 Cys Thr Glu Ser Ala Asp Cys Pro Ser Thr Cys SerThr Cys Leu His 65 70 75 gct caa tgc gag tca aca tgatgtcgca ctacagctcttctctacagt 288 Ala Gln Cys Glu Ser Thr 80 gtgtacatcg accgtacgacgcatctttta tttctttggc tgtttcattc gttttcttgt 348 gttcataaca tgcggagcccttccgttacc tctactgctc tacacttaac ctgataacca 408 gaaaatccag tact 422 8684 PRT Conus caracteristicus 86 Met His Leu Ser Leu Ala Arg Ser Ala ValLeu Met Leu Leu Leu Leu 1 5 10 15 Phe Ala Leu Asp Asn Phe Val Gly ValGln Pro Gly Gln Ile Thr Arg 20 25 30 Asp Val Asp Asn Arg Arg Asn Arg GlnSer Arg Trp Lys Pro Arg Ser 35 40 45 Leu Phe Lys Ser Leu His Lys Arg AlaSer Cys Gly Gly Thr Cys Thr 50 55 60 Glu Ser Ala Asp Cys Pro Ser Thr CysSer Thr Cys Leu His Ala Gln 65 70 75 80 Cys Glu Ser Thr 87 28 PRT Conuscaracteristicus PEPTIDE (1)..(28) Xaa at residues 9 and 26 is Glu orgamma-carboxy-Glu; Xaa at residue 14 is Pro or hydroxy-Pro 87 Ala SerCys Gly Gly Thr Cys Thr Xaa Ser Ala Asp Cys Xaa Ser Thr 1 5 10 15 CysSer Thr Cys Leu His Ala Gln Cys Xaa Ser Thr 20 25 88 426 DNA Conuscaracteristicus CDS (7)..(258) 88 gttaca atg cat ctg tca ctg gca cgc tcagct gtt ttg atg ttg ctt 48 Met His Leu Ser Leu Ala Arg Ser Ala Val LeuMet Leu Leu 1 5 10 ctg ctg ttt gcc ttg gac aac ttc gtt ggg gtc caa ccagga cag ata 96 Leu Leu Phe Ala Leu Asp Asn Phe Val Gly Val Gln Pro GlyGln Ile 15 20 25 30 act aga gat gtg gac aac cgc cgt aac ctg caa tcg cgatgg aag cca 144 Thr Arg Asp Val Asp Asn Arg Arg Asn Leu Gln Ser Arg TrpLys Pro 35 40 45 agg agt ctc ttc aag tca ctt cat aaa cga gca tcg tgt ggaggg act 192 Arg Ser Leu Phe Lys Ser Leu His Lys Arg Ala Ser Cys Gly GlyThr 50 55 60 tgc acg gaa agt gcc gat tgc cct tcc acg tgt agt act tgc ttacat 240 Cys Thr Glu Ser Ala Asp Cys Pro Ser Thr Cys Ser Thr Cys Leu His65 70 75 gct caa tgc gag tga aca tgatgtcgca ctacagctct tctctacagt 288Ala Gln Cys Glu Thr 80 gtgtacatcg accgaccgta cgacgcatct tttatttctttgtctgtttc attcgttttc 348 ttgagttcat aacatgcgga gcccttccgt tacctctactgctctacact taagctgata 408 accagaaaat ccagtact 426 89 82 PRT Conuscaracteristicus 89 Met His Leu Ser Leu Ala Arg Ser Ala Val Leu Met LeuLeu Leu Leu 1 5 10 15 Phe Ala Leu Asp Asn Phe Val Gly Val Gln Pro GlyGln Ile Thr Arg 20 25 30 Asp Val Asp Asn Arg Arg Asn Leu Gln Ser Arg TrpLys Pro Arg Ser 35 40 45 Leu Phe Lys Ser Leu His Lys Arg Ala Ser Cys GlyGly Thr Cys Thr 50 55 60 Glu Ser Ala Asp Cys Pro Ser Thr Cys Ser Thr CysLeu His Ala Gln 65 70 75 80 Cys Glu 90 25 PRT Conus caracteristicusPEPTIDE (1)..(25) Xaa at residues 8 and 25 is Glu or gamma-carboxy-Glu;Xaa at residue 13 is Pro or hydroxy-Pro 90 Ser Cys Gly Gly Thr Cys ThrXaa Ser Ala Asp Cys Xaa Ser Thr Cys 1 5 10 15 Ser Thr Cys Leu His AlaGln Cys Xaa 20 25 91 220 DNA Conus circumcisus CDS (28)..(189) 91tgtgtgtgtg tggttctggg tccagca ttt gat ggc agg aat gcc gca gtc aac 54 PheAsp Gly Arg Asn Ala Ala Val Asn 1 5 gag aga gcg cct tgg acg gtc gtt ttgtcc acc acg aat tgc tgc ggt 102 Glu Arg Ala Pro Trp Thr Val Val Leu SerThr Thr Asn Cys Cys Gly 10 15 20 25 tat aat acg atg gaa ttc tgc cct gcttgc atg tgc act tat tcc tgt 150 Tyr Asn Thr Met Glu Phe Cys Pro Ala CysMet Cys Thr Tyr Ser Cys 30 35 40 cca aaa aag aaa aaa cca gga aaa ggc cgcaga aac aac tgatgctcca 199 Pro Lys Lys Lys Lys Pro Gly Lys Gly Arg ArgAsn Asn 45 50 ggaccctctg aaccacgacg t 220 92 54 PRT Conus circumcisus 92Phe Asp Gly Arg Asn Ala Ala Val Asn Glu Arg Ala Pro Trp Thr Val 1 5 1015 Val Leu Ser Thr Thr Asn Cys Cys Gly Tyr Asn Thr Met Glu Phe Cys 20 2530 Pro Ala Cys Met Cys Thr Tyr Ser Cys Pro Lys Lys Lys Lys Pro Gly 35 4045 Lys Gly Arg Arg Asn Asn 50 93 43 PRT Conus circumcisus PEPTIDE(1)..(43) Xaa at residue 19 is Glu or gamma-carboxy-Glu; Xaa at residues2, 22, 31 and 36 is Pro or hydroxy-Pro; Xaa at residue 3 is Trp (D or L)or bromo-Trp (D or L); Xaa at residues 15 and 28 is Tyr, 125 I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 93 Ala Xaa XaaThr Val Val Leu Ser Thr Thr Asn Cys Cys Gly Xaa Asn 1 5 10 15 Thr MetXaa Phe Cys Xaa Ala Cys Met Cys Thr Xaa Ser Cys Xaa Lys 20 25 30 Lys LysLys Xaa Gly Lys Gly Arg Arg Asn Asn 35 40 94 64 PRT Conus consors 94 GlyIle Phe Val Gly Val Gln Pro Glu Gln Ile Thr Arg Asp Val Asp 1 5 10 15Lys Gly Tyr Ser Thr Asp Asp Gly His Asp Leu Leu Ser Leu Leu Lys 20 25 30Gln Ile Ser Leu Arg Ala Cys Thr Gly Ser Cys Asn Ser Asp Ser Glu 35 40 45Cys Tyr Asn Phe Cys Asp Cys Ile Gly Thr Arg Cys Glu Ala Gln Lys 50 55 6095 27 PRT Conus consors PEPTIDE (1)..(27) Xaa at residues 11 and 24 isGlu or gamma-carboxy-Glu; Xaa at residue 13 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulph0-Tyr or O-phospho-Tyr 95 Ala Cys ThrGly Ser Cys Asn Ser Asp Ser Xaa Cys Xaa Asn Phe Cys 1 5 10 15 Asp CysIle Gly Thr Arg Cys Xaa Ala Gln Lys 20 25 96 27 PRT Conus delesstiiPEPTIDE (1)..(27) Xaa at residues 13 and 16 is Glu or gamma-carboxy-Glu;Xaa at residue 4 is Pro or hydroxy-Pro; Xaa at residue 25 is Tyr,125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 96Ala Cys Lys Xaa Lys Asn Asn Leu Cys Ala Ile Thr Xaa Met Ala Xaa 1 5 1015 Cys Cys Ser Gly Phe Cys Leu Ile Xaa Arg Cys 20 25 97 375 DNA Conusdistans CDS (24)..(260) 97 gacaggattg aacaaaattc agg atg tca gga ttg ggaatc atg gtg cta acc 53 Met Ser Gly Leu Gly Ile Met Val Leu Thr 1 5 10ctt cta ctt ctt gtg ccc atg gca acc agt caa cag gat gga gga gag 101 LeuLeu Leu Leu Val Pro Met Ala Thr Ser Gln Gln Asp Gly Gly Glu 15 20 25 aagcag gcg atg caa agg gac gca atc aac gtc gca cca gga aca tca 149 Lys GlnAla Met Gln Arg Asp Ala Ile Asn Val Ala Pro Gly Thr Ser 30 35 40 atc actcgg aga aat gta gat cag gag tgc att gac gcc tgt cag ctg 197 Ile Thr ArgArg Asn Val Asp Gln Glu Cys Ile Asp Ala Cys Gln Leu 45 50 55 gag gac aagaat tgc tgt ggc aga aca gat gga gaa ccc aga tgt gcg 245 Glu Asp Lys AsnCys Cys Gly Arg Thr Asp Gly Glu Pro Arg Cys Ala 60 65 70 aaa atc tgc ctcgga taatttctgt acgctgtctc attcattatt tcatccgtac 300 Lys Ile Cys Leu Gly75 gagtgtaaac gagacctatt agaaagtcga aggttgtgcg taatttgata agcattgttt 360gctgggacga acgga 375 98 79 PRT Conus distans 98 Met Ser Gly Leu Gly IleMet Val Leu Thr Leu Leu Leu Leu Val Pro 1 5 10 15 Met Ala Thr Ser GlnGln Asp Gly Gly Glu Lys Gln Ala Met Gln Arg 20 25 30 Asp Ala Ile Asn ValAla Pro Gly Thr Ser Ile Thr Arg Arg Asn Val 35 40 45 Asp Gln Glu Cys IleAsp Ala Cys Gln Leu Glu Asp Lys Asn Cys Cys 50 55 60 Gly Arg Thr Asp GlyGlu Pro Arg Cys Ala Lys Ile Cys Leu Gly 65 70 75 99 32 PRT Conus distansPEPTIDE (1)..(32) Xaa at residues 5, 13 and 24 is Glu orgamma-carboxy-Glu; Xaa at residue 25 is Pro or hydroxy-Pro 99 Asn ValAsp Gln Xaa Cys Ile Asp Ala Cys Gln Leu Xaa Asp Lys Asn 1 5 10 15 CysCys Gly Arg Thr Asp Gly Xaa Xaa Arg Cys Ala Lys Ile Cys Leu 20 25 30 100376 DNA Conus distans CDS (24)..(260) 100 gacaggattg aacaaaattc agg atgtca gga ttg gga atc atg gtg cta acc 53 Met Ser Gly Leu Gly Ile Met ValLeu Thr 1 5 10 ctt cta ctt ctt gtg ccc atg gca acc agt caa cag gat ggagga gag 101 Leu Leu Leu Leu Val Pro Met Ala Thr Ser Gln Gln Asp Gly GlyGlu 15 20 25 aag cag gcg atg caa agg gac gca atc aac gtc gca cca gga acatca 149 Lys Gln Ala Met Gln Arg Asp Ala Ile Asn Val Ala Pro Gly Thr Ser30 35 40 atc act cgg aca gaa aca gat cag gag tgc att gac atc tgt aag cag197 Ile Thr Arg Thr Glu Thr Asp Gln Glu Cys Ile Asp Ile Cys Lys Gln 4550 55 gag gac aag aaa tgc tgc ggc aga tca aat gga gaa ccc aca tgt gcg245 Glu Asp Lys Lys Cys Cys Gly Arg Ser Asn Gly Glu Pro Thr Cys Ala 6065 70 aaa atc tgc ctc gga taatttctgt acgctgtctc gttcattatt tcgtcagtac300 Lys Ile Cys Leu Gly 75 gagtttaaac gagacctatt agaaagtcga aggttcgtgcttaatttgat aagcattgtt 360 tgctgggatg aacgga 376 101 79 PRT Conus distans101 Met Ser Gly Leu Gly Ile Met Val Leu Thr Leu Leu Leu Leu Val Pro 1 510 15 Met Ala Thr Ser Gln Gln Asp Gly Gly Glu Lys Gln Ala Met Gln Arg 2025 30 Asp Ala Ile Asn Val Ala Pro Gly Thr Ser Ile Thr Arg Thr Glu Thr 3540 45 Asp Gln Glu Cys Ile Asp Ile Cys Lys Gln Glu Asp Lys Lys Cys Cys 5055 60 Gly Arg Ser Asn Gly Glu Pro Thr Cys Ala Lys Ile Cys Leu Gly 65 7075 102 32 PRT Conus distans PEPTIDE (1)..(32) Xaa at residues 1, 5, 13and 24 is Glu or gamma-carboxy-Glu; Xaa at residue 25 is Pro orhydroxy-Pro 102 Xaa Thr Asp Gln Xaa Cys Ile Asp Ile Cys Lys Gln Xaa AspLys Lys 1 5 10 15 Cys Cys Gly Arg Ser Asn Gly Xaa Xaa Thr Cys Ala LysIle Cys Leu 20 25 30 103 376 DNA Conus distans CDS (24)..(260) 103gacaggattg aacaaaattc agg atg tca gga ttg gga atc atg gtg cta acc 53 MetSer Gly Leu Gly Ile Met Val Leu Thr 1 5 10 ctt cta ctt ctt gtg ccc atggca acc agt caa cag gat gga gga gag 101 Leu Leu Leu Leu Val Pro Met AlaThr Ser Gln Gln Asp Gly Gly Glu 15 20 25 aag cag gcg atg caa agg gac gcaatc aac gtc gca cca gga aca tca 149 Lys Gln Ala Met Gln Arg Asp Ala IleAsn Val Ala Pro Gly Thr Ser 30 35 40 atc act cgg aga gaa aca gat cag gagtgc att gac acc tgt gag cag 197 Ile Thr Arg Arg Glu Thr Asp Gln Glu CysIle Asp Thr Cys Glu Gln 45 50 55 gag gac aag aaa tgc tgc ggc aga aca aatgga gaa ccc gta tgt gcg 245 Glu Asp Lys Lys Cys Cys Gly Arg Thr Asn GlyGlu Pro Val Cys Ala 60 65 70 aaa atc tgc ttc gga taatttctgt acgctgtctcattcataatt tcatcagtac 300 Lys Ile Cys Phe Gly 75 gagtttaaac gagacctattagaaagtcga aggttcgtgc ttaatttgat aagcattgtt 360 tgctgggatg aacgga 376104 79 PRT Conus distans 104 Met Ser Gly Leu Gly Ile Met Val Leu Thr LeuLeu Leu Leu Val Pro 1 5 10 15 Met Ala Thr Ser Gln Gln Asp Gly Gly GluLys Gln Ala Met Gln Arg 20 25 30 Asp Ala Ile Asn Val Ala Pro Gly Thr SerIle Thr Arg Arg Glu Thr 35 40 45 Asp Gln Glu Cys Ile Asp Thr Cys Glu GlnGlu Asp Lys Lys Cys Cys 50 55 60 Gly Arg Thr Asn Gly Glu Pro Val Cys AlaLys Ile Cys Phe Gly 65 70 75 105 32 PRT Conus distans PEPTIDE (1)..(32)Xaa at residues 1, 5, 11, 13 and 24 is Glu or gamma-carboxy-Glu; Xaa atresidue 25 is Pro or hydroxy-Pro 105 Xaa Thr Asp Gln Xaa Cys Ile Asp ThrCys Xaa Gln Xaa Asp Lys Lys 1 5 10 15 Cys Cys Gly Arg Thr Asn Gly XaaXaa Val Cys Ala Lys Ile Cys Phe 20 25 30 106 250 DNA Conus ermineus CDS(1)..(219) 106 atg ttc acc gtg ttt ctg ttg gtt gtc ttg gca acc act gtcgtt tcc 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val ValSer 1 5 10 15 ttc act tca gat cgt gca tcg gat gac agg aat acc aac gacaaa gca 96 Phe Thr Ser Asp Arg Ala Ser Asp Asp Arg Asn Thr Asn Asp LysAla 20 25 30 tct cgc ctg ctc tct cac gtt gtc agg gga tgc tgt ggt aag tatccc 144 Ser Arg Leu Leu Ser His Val Val Arg Gly Cys Cys Gly Lys Tyr Pro35 40 45 aat gct gcc tgt cat cct tgc ggt tgt aca gtg ggt agg cca ccg tat192 Asn Ala Ala Cys His Pro Cys Gly Cys Thr Val Gly Arg Pro Pro Tyr 5055 60 tgt gac aga ccc agt ggt gga gga cgc tgatgctcca ggaccctctg 239 CysAsp Arg Pro Ser Gly Gly Gly Arg 65 70 aaccacgacg t 250 107 73 PRT Conusermineus 107 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val ValSer 1 5 10 15 Phe Thr Ser Asp Arg Ala Ser Asp Asp Arg Asn Thr Asn AspLys Ala 20 25 30 Ser Arg Leu Leu Ser His Val Val Arg Gly Cys Cys Gly LysTyr Pro 35 40 45 Asn Ala Ala Cys His Pro Cys Gly Cys Thr Val Gly Arg ProPro Tyr 50 55 60 Cys Asp Arg Pro Ser Gly Gly Gly Arg 65 70 108 30 PRTConus ermineus PEPTIDE (1)..(30) Xaa at residue7, 13, 21, 22 and 27 isPro or hydroxy-Pro; Xaa at residues 6 and 23 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 108 Gly CysCys Gly Lys Xaa Xaa Asn Ala Ala Cys His Xaa Cys Gly Cys 1 5 10 15 ThrVal Gly Arg Xaa Xaa Xaa Cys Asp Arg Xaa Ser Gly Gly 20 25 30 109 241 DNAConus generalis CDS (1)..(210) 109 gga tcc atg atg tct aaa ctg gga gtcttg ttg acc atc tgt ctg gtt 48 Gly Ser Met Met Ser Lys Leu Gly Val LeuLeu Thr Ile Cys Leu Val 1 5 10 15 ctg ttt ccc ctt act gct ctt cca ctggat gga gaa caa cct gta gac 96 Leu Phe Pro Leu Thr Ala Leu Pro Leu AspGly Glu Gln Pro Val Asp 20 25 30 cga cat gcc gag cat atg cag gat gac aattca gct gca cag aac ccc 144 Arg His Ala Glu His Met Gln Asp Asp Asn SerAla Ala Gln Asn Pro 35 40 45 tgg gtt att gcc atc aga cag tgt tgc acg ttctgc aac ttt gga tgc 192 Trp Val Ile Ala Ile Arg Gln Cys Cys Thr Phe CysAsn Phe Gly Cys 50 55 60 cag cct tgt tgc gtc ccc tgataacgtg ttgatgaccaactttctcga g 241 Gln Pro Cys Cys Val Pro 65 70 110 70 PRT Conusgeneralis 110 Gly Ser Met Met Ser Lys Leu Gly Val Leu Leu Thr Ile CysLeu Val 1 5 10 15 Leu Phe Pro Leu Thr Ala Leu Pro Leu Asp Gly Glu GlnPro Val Asp 20 25 30 Arg His Ala Glu His Met Gln Asp Asp Asn Ser Ala AlaGln Asn Pro 35 40 45 Trp Val Ile Ala Ile Arg Gln Cys Cys Thr Phe Cys AsnPhe Gly Cys 50 55 60 Gln Pro Cys Cys Val Pro 65 70 111 16 PRT Conusgeneralis PEPTIDE (1)..(16) Xaa at residue 1 is Gln or pyro-Gln; Xaa atresidues 12 and 16 is Pro or hydroxy-Pro 111 Xaa Cys Cys Thr Phe Cys AsnPhe Gly Cys Gln Xaa Cys Cys Val Xaa 1 5 10 15 112 404 DNA Conusgeographus CDS (18)..(242) 112 gcaagatcat cagcaga atg aac ctg acg tgcgtg ttg atc atc gcc gtg 50 Met Asn Leu Thr Cys Val Leu Ile Ile Ala Val 15 10 ctg ttt ctg acg gcc tgc cag ctc att gca gct gat gac tcc aga gat 98Leu Phe Leu Thr Ala Cys Gln Leu Ile Ala Ala Asp Asp Ser Arg Asp 15 20 25aac cag aag cac cgt gca gtg agg atg aga gac gca ttg aag aat ttc 146 AsnGln Lys His Arg Ala Val Arg Met Arg Asp Ala Leu Lys Asn Phe 30 35 40 aaagat tcc agg gcg tgc tcc ggt aga ggt tct aga tgt cct ccc caa 194 Lys AspSer Arg Ala Cys Ser Gly Arg Gly Ser Arg Cys Pro Pro Gln 45 50 55 tgc tgcatg ggt ttg acg tgc ggt cgt gag tat cca ccc aga tgc ggt 242 Cys Cys MetGly Leu Thr Cys Gly Arg Glu Tyr Pro Pro Arg Cys Gly 60 65 70 75tgatatacgg tgaacaactg atatttcccc tctgtgctct accctctttt gcctgattca 302cccacaccta tgtgtggtca tgaaccactc agtacctaca cctctggtgg cttcagagga 362cgtatattaa aataaaacca cattgcaatg aaaaaaaaaa aa 404 113 75 PRT Conusgeographus 113 Met Asn Leu Thr Cys Val Leu Ile Ile Ala Val Leu Phe LeuThr Ala 1 5 10 15 Cys Gln Leu Ile Ala Ala Asp Asp Ser Arg Asp Asn GlnLys His Arg 20 25 30 Ala Val Arg Met Arg Asp Ala Leu Lys Asn Phe Lys AspSer Arg Ala 35 40 45 Cys Ser Gly Arg Gly Ser Arg Cys Pro Pro Gln Cys CysMet Gly Leu 50 55 60 Thr Cys Gly Arg Glu Tyr Pro Pro Arg Cys Gly 65 7075 114 27 PRT Conus geographus PEPTIDE (1)..(27) Xaa at residue 22 isGlu or gamma-carboxy-Glu; Xaa at residues 10, 11, 24 and 25 is Pro orhydroxy-Pro; Xaa at residue 23 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 114 Ala Cys Ser Gly Arg GlySer Arg Cys Xaa Xaa Gln Cys Cys Met Gly 1 5 10 15 Leu Thr Cys Gly ArgXaa Xaa Xaa Xaa Arg Cys 20 25 115 9 PRT Conus geographus PEPTIDE(1)..(9) Xaa at residue 7 is Pro or hydroxy-Pro 115 Cys Phe Ile Arg AsnCys Xaa Lys Gly 1 5 116 360 DNA Conus geographus CDS (1)..(213) 116 tgctgc ccg agt agc aaa gag gat tcc ctg aac tgc att gag acc atg 48 Cys CysPro Ser Ser Lys Glu Asp Ser Leu Asn Cys Ile Glu Thr Met 1 5 10 15 gcgacc acg gcc acg tgc atg aag tcc aac aag ggg gag atc tac tcc 96 Ala ThrThr Ala Thr Cys Met Lys Ser Asn Lys Gly Glu Ile Tyr Ser 20 25 30 tat gcgtgc ggc tac tgc ggc aag aag aag gag agc tgt ttc ggc gac 144 Tyr Ala CysGly Tyr Cys Gly Lys Lys Lys Glu Ser Cys Phe Gly Asp 35 40 45 aaa aag ccagtg act gac tac cag tgc cag acg cgg aac att ccc aac 192 Lys Lys Pro ValThr Asp Tyr Gln Cys Gln Thr Arg Asn Ile Pro Asn 50 55 60 ccc tgc ggc ggcgct gct ctc tgaaggcacc aacagcacca acagcacgat 243 Pro Cys Gly Gly Ala AlaLeu 65 70 ctcctgtgtt tcgtcactgc atttatgacg tcaaaaccac gtcatgcatgatgacgacga 303 tctcggctat ggcatgtatt gaagaatgga aataaaccta gttttcagctgaaaaaa 360 117 71 PRT Conus geographus 117 Cys Cys Pro Ser Ser Lys GluAsp Ser Leu Asn Cys Ile Glu Thr Met 1 5 10 15 Ala Thr Thr Ala Thr CysMet Lys Ser Asn Lys Gly Glu Ile Tyr Ser 20 25 30 Tyr Ala Cys Gly Tyr CysGly Lys Lys Lys Glu Ser Cys Phe Gly Asp 35 40 45 Lys Lys Pro Val Thr AspTyr Gln Cys Gln Thr Arg Asn Ile Pro Asn 50 55 60 Pro Cys Gly Gly Ala AlaLeu 65 70 118 71 PRT Conus geographus PEPTIDE (1)..(71) Xaa at residues7, 14, 29 and 43 is Glu or gamma-carboxy-Glu; Xaa at residues 3, 51, 63and 65 is Pro or hydroxy-Pro; Xaa at residues 31, 33, 37 and 55 is Tyr,125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 118Cys Cys Xaa Ser Ser Lys Xaa Asp Ser Leu Asn Cys Ile Xaa Thr Met 1 5 1015 Ala Thr Thr Ala Thr Cys Met Lys Ser Asn Lys Gly Xaa Ile Xaa Ser 20 2530 Xaa Ala Cys Gly Xaa Cys Gly Lys Lys Lys Xaa Ser Cys Phe Gly Asp 35 4045 Lys Lys Xaa Val Thr Asp Xaa Gln Cys Gln Thr Arg Asn Ile Xaa Asn 50 5560 Xaa Cys Gly Gly Ala Ala Leu 65 70 119 769 DNA Conus geographusmisc_feature (1)..(769) n is unknown 119 cgggcgctgc attccggacgtgaaacagca tcgccagcaa gtgggcatag tgcaagacac 60 tcagaacaat gacgcacatagtctganaaa ataaccatgg gtatgcggat gangtttagt 120 gtgtttcngc aggttgtcntgggnaccact gtcgtttcct tcacntcacg tcgtggtcca 180 aaatctcgtc gcggggaacctattccgacc actgtaatca actacgggga gtgctgtaag 240 gatccatcct gttgggttaaggtgaaggat ttccagtgtc ctggagcaag tcctcccaac 300 tgaaccacga catgtcgccctctgcctgac ctgcttcacg ttccgtctct ttctgccact 360 agaactcaac aactcgatccaacagactcc tactttacct ccgtattctg aaactacttg 420 gatttgattg tctttaatatctactcacac ttgctgttat tacatcatcc aaaatttaac 480 aagaacatga aaggtgtctgttcaaacaaa atcaggcaat gacaangggg gaaagtctcc 540 antctatctg aaaactgtcacctgtcactc tcttaaccag gtttanaact gantaccact 600 anagctgttg tnccacatcangatcagncc aatttgtann gtttcctttg caaaactttt 660 gcctgaaatt cttgaaaagaaacgctcaca atgttgggaa gtgcttttna ttanctgaca 720 anntgncanc atgttccntttcantaantc tnaaatgnaa acctctgtt 769 120 68 PRT Conus geographus 120 MetGly Met Arg Met Met Phe Ser Val Phe Leu Gln Val Val Leu Gly 1 5 10 15Thr Thr Val Val Ser Phe Thr Ser Arg Arg Gly Pro Lys Ser Arg Arg 20 25 30Gly Glu Pro Ile Pro Thr Thr Val Ile Asn Tyr Gly Glu Cys Cys Lys 35 40 45Asp Pro Ser Cys Trp Val Lys Val Lys Asp Phe Gln Cys Pro Gly Ala 50 55 60Ser Pro Pro Asn 65 121 36 PRT Conus geographus PEPTIDE (1)..(36) Xaa atresidues 2 and 13 is Glu or gamma-carboxy-Glu; Xaa at residues 3, 5, 18,30, 35 adn 35 is Pro or hydroxy-Pro; Xaa at residue 21 is Trp (D or L)or bromo-Trp (D or L); 121 Gly Xaa Xaa Ile Xaa Thr Thr Val Ile Asn XaaGly Xaa Cys Cys Lys 1 5 10 15 Asp Xaa Ser Cys Xaa Val Lys Val Lys AspPhe Gln Cys Xaa Gly Ala 20 25 30 Ser Xaa Xaa Asn 35 122 519 DNA Conusgeographus CDS (113)..(391) 122 agccttgata cagagctggt atctgctgttaatacttgaa agaacaagtg ctgtgagcct 60 tcatctctct ctgactttag tttgggtcctggagaaaacc ttgacgggca gt atg aaa 118 Met Lys 1 att tac ctg tgt ctt gctttt gtt ctg ctc ctg gct tct acc ata gtt 166 Ile Tyr Leu Cys Leu Ala PheVal Leu Leu Leu Ala Ser Thr Ile Val 5 10 15 gat tca ggg ctt ctt gat aaaatt gag act ata aga aac tgg aaa cgc 214 Asp Ser Gly Leu Leu Asp Lys IleGlu Thr Ile Arg Asn Trp Lys Arg 20 25 30 gat gac agc tat tgt gat gga tgccta tgc acc ata tta aaa aaa gag 262 Asp Asp Ser Tyr Cys Asp Gly Cys LeuCys Thr Ile Leu Lys Lys Glu 35 40 45 50 act tgc aca tcg act atg agc tgcagg gga aca tgc cga aaa gag tgg 310 Thr Cys Thr Ser Thr Met Ser Cys ArgGly Thr Cys Arg Lys Glu Trp 55 60 65 cca tgt tgg gaa gaa gac tgc tac tgtact gaa atc caa ggt gga gct 358 Pro Cys Trp Glu Glu Asp Cys Tyr Cys ThrGlu Ile Gln Gly Gly Ala 70 75 80 tgc gtc aca ccc tca gaa tgc aaa cct ggagag tgttgaggat tggagtggcc 411 Cys Val Thr Pro Ser Glu Cys Lys Pro GlyGlu 85 90 agttccagca catacagcac catggtgccc tggacaatcg tctattgaattgaatatgcc 471 tgtggcagga atctgtccta caaaataaaa aaatcataag ttaaaaaa 519123 93 PRT Conus geographus 123 Met Lys Ile Tyr Leu Cys Leu Ala Phe ValLeu Leu Leu Ala Ser Thr 1 5 10 15 Ile Val Asp Ser Gly Leu Leu Asp LysIle Glu Thr Ile Arg Asn Trp 20 25 30 Lys Arg Asp Asp Ser Tyr Cys Asp GlyCys Leu Cys Thr Ile Leu Lys 35 40 45 Lys Glu Thr Cys Thr Ser Thr Met SerCys Arg Gly Thr Cys Arg Lys 50 55 60 Glu Trp Pro Cys Trp Glu Glu Asp CysTyr Cys Thr Glu Ile Gln Gly 65 70 75 80 Gly Ala Cys Val Thr Pro Ser GluCys Lys Pro Gly Glu 85 90 124 60 PRT Conus geographus PEPTIDE (1)..(60)Xaa at residues 16, 36, 37, 43, 56 and 59 is Glu or gamma- carboxy-Glu;Xaa at residues 33, 52 and 57 is Pro or hydroxy-Pro; Xaa at residues 32and 35 is Trp (D or L) or bromo-Trp (D or L); 124 Asp Asp Ser Xaa CysAsp Gly Cys Leu Cys Thr Ile Leu Lys Lys Xaa 1 5 10 15 Thr Cys Thr SerThr Met Ser Cys Arg Gly Thr Cys Arg Lys Xaa Xaa 20 25 30 Xaa Cys Xaa XaaXaa Asp Cys Xaa Cys Thr Xaa Ile Gln Gly Gly Ala 35 40 45 Cys Val Thr XaaSer Xaa Cys Lys Xaa Gly Xaa Cys 50 55 60 125 409 DNA Conus geographusCDS (17)..(313) 125 aacgttgacg ggcagt atg aac att tac ctg tgt ctt gctttt ctt ctg ttc 52 Met Asn Ile Tyr Leu Cys Leu Ala Phe Leu Leu Phe 1 510 ctg cct tct acc ata gtt gat tca ggg ctt ctt gat aaa att gag aca 100Leu Pro Ser Thr Ile Val Asp Ser Gly Leu Leu Asp Lys Ile Glu Thr 15 20 25ata agg aat tgg aga cgt gat gaa agc aag tgt gat cga tgc aat tgc 148 IleArg Asn Trp Arg Arg Asp Glu Ser Lys Cys Asp Arg Cys Asn Cys 30 35 40 gccgaa tta aga tca tcc aga tgc aca caa gct atc ttc tgc ctt aca 196 Ala GluLeu Arg Ser Ser Arg Cys Thr Gln Ala Ile Phe Cys Leu Thr 45 50 55 60 ccggag tta tgc aca ccg agc atc tca tgt ccg aca ggt gaa tgc cgc 244 Pro GluLeu Cys Thr Pro Ser Ile Ser Cys Pro Thr Gly Glu Cys Arg 65 70 75 tgt actaag ttc cat cag tca aga tgc act aga ttc gta gaa tgc gta 292 Cys Thr LysPhe His Gln Ser Arg Cys Thr Arg Phe Val Glu Cys Val 80 85 90 cct aat aagtgt aga gac gca tagaggccag ttccagcaca tacagcacca 343 Pro Asn Lys Cys ArgAsp Ala 95 tgatgccctg gacaatcgtg ttgttggatt gaatatgccc gtggcaggaatctgtcctac 403 aaaaaa 409 126 99 PRT Conus geographus 126 Met Asn IleTyr Leu Cys Leu Ala Phe Leu Leu Phe Leu Pro Ser Thr 1 5 10 15 Ile ValAsp Ser Gly Leu Leu Asp Lys Ile Glu Thr Ile Arg Asn Trp 20 25 30 Arg ArgAsp Glu Ser Lys Cys Asp Arg Cys Asn Cys Ala Glu Leu Arg 35 40 45 Ser SerArg Cys Thr Gln Ala Ile Phe Cys Leu Thr Pro Glu Leu Cys 50 55 60 Thr ProSer Ile Ser Cys Pro Thr Gly Glu Cys Arg Cys Thr Lys Phe 65 70 75 80 HisGln Ser Arg Cys Thr Arg Phe Val Glu Cys Val Pro Asn Lys Cys 85 90 95 ArgAsp Ala 127 65 PRT Conus geographus PEPTIDE (1)..(65) Xaa at residues 2,12, 28, 40 and 56 is Glu or gamma-carboxy-Glu; Xaa at residues 27, 32,37 and 59 is Pro or hydroxy-Pro 127 Asp Xaa Ser Lys Cys Asp Arg Cys AsnCys Ala Xaa Leu Arg Ser Ser 1 5 10 15 Arg Cys Thr Gln Ala Ile Phe CysLeu Thr Xaa Xaa Leu Cys Thr Xaa 20 25 30 Ser Ile Ser Cys Xaa Thr Gly XaaCys Arg Cys Thr Lys Phe His Gln 35 40 45 Ser Arg Cys Thr Arg Phe Val XaaCys Val Xaa Asn Lys Cys Arg Asp 50 55 60 Ala 65 128 29 PRT Conusgeographus PEPTIDE (1)..(29) Xaa at residues 9 and 23 is Glu orgamma-carboxy-Glu; Xaa at residue 28 is Pro or hydroxy-Pro; Xaa atresidue 15 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 128 Lys Phe Leu Ser Gly Gly Phe Lys Xaa Ile Val Cys HisArg Xaa Cys 1 5 10 15 Ala Lys Gly Ile Ala Lys Xaa Phe Cys Asn Cys XaaAsp 20 25 129 7 PRT Conus imperialis PEPTIDE (1)..(7) Xaa at residue 1is Gln or pyro-Gln; Xaa at residue 6 is Trp (D or L) or bromo-Trp (D orL) 129 Xaa Cys Gly Gln Ala Xaa Cys 1 5 130 524 DNA Conus imperialis CDS(7)..(285) 130 gttaaa atg cat ctg tca ctg gca agc tca gct gct ttg atgttg ctt 48 Met His Leu Ser Leu Ala Ser Ser Ala Ala Leu Met Leu Leu 1 510 ctg ctt ttt gcc ttg ggc aac ttc gtt ggg gtc cag cca gga caa ata 96Leu Leu Phe Ala Leu Gly Asn Phe Val Gly Val Gln Pro Gly Gln Ile 15 20 2530 aga gat ctg aac aaa gga cag ctc aag gac aac cgc cgt aac ctg caa 144Arg Asp Leu Asn Lys Gly Gln Leu Lys Asp Asn Arg Arg Asn Leu Gln 35 40 45tcg cag agg aaa caa atg agt ctc ctc aag tca ctt cat gat cga aat 192 SerGln Arg Lys Gln Met Ser Leu Leu Lys Ser Leu His Asp Arg Asn 50 55 60 gggtgt aac ggc aac acg tgt tcc aat agc ccc tgc cct aac aac tgt 240 Gly CysAsn Gly Asn Thr Cys Ser Asn Ser Pro Cys Pro Asn Asn Cys 65 70 75 tat tgcgat act gag gac gac tgc cac cct gac agg cgt gaa cat 285 Tyr Cys Asp ThrGlu Asp Asp Cys His Pro Asp Arg Arg Glu His 80 85 90 tagagattagagagtttcct tgtcaacatg atgtcgcacc acacctctgc tctgcagtgt 345 gtacatcgaccagtcgacgc atctgttatt tctttgtctg ttggattgta catcgaccag 405 tccacgcatctgttatttct ttgtctgttt gatttgtttt cgtgtgttca taacacacag 465 agcctttctattatctgtat tgcaatacac tttgcctgat aaccagaaag tccagtgct 524 131 93 PRTConus imperialis 131 Met His Leu Ser Leu Ala Ser Ser Ala Ala Leu Met LeuLeu Leu Leu 1 5 10 15 Phe Ala Leu Gly Asn Phe Val Gly Val Gln Pro GlyGln Ile Arg Asp 20 25 30 Leu Asn Lys Gly Gln Leu Lys Asp Asn Arg Arg AsnLeu Gln Ser Gln 35 40 45 Arg Lys Gln Met Ser Leu Leu Lys Ser Leu His AspArg Asn Gly Cys 50 55 60 Asn Gly Asn Thr Cys Ser Asn Ser Pro Cys Pro AsnAsn Cys Tyr Cys 65 70 75 80 Asp Thr Glu Asp Asp Cys His Pro Asp Arg ArgGlu His 85 90 132 32 PRT Conus imperialis PEPTIDE (1)..(32) Xaa atresidues 22 and 31 is Glu or gamma-carboxy-Glu; Xaa at residues 13, 14and 27 is Pro or hydroxy-Pro; Xaa at residue 18 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 132 Asn GlyCys Asn Gly Asn Thr Cys Ser Asn Ser Xaa Cys Xaa Asn Asn 1 5 10 15 CysXaa Cys Asp Thr Xaa Asp Asp Cys His Xaa Asp Arg Arg Xaa His 20 25 30 133350 DNA Conus lacterculatus CDS (1)..(273) 133 atg atg tcg aaa atg ggagct atg ttt gtc ctt ttg ctt ctt ttc acc 48 Met Met Ser Lys Met Gly AlaMet Phe Val Leu Leu Leu Leu Phe Thr 1 5 10 15 ctg gca tcc agc cag caggaa gga gat gtc cag gca agg aaa aca cac 96 Leu Ala Ser Ser Gln Gln GluGly Asp Val Gln Ala Arg Lys Thr His 20 25 30 ccg aag aga gag ttc cat cgtatt ctg cta agg cct gac aga cag tcc 144 Pro Lys Arg Glu Phe His Arg IleLeu Leu Arg Pro Asp Arg Gln Ser 35 40 45 gaa acg gct tgt agg tcg ctc ggaagc tac caa tgt atg ggt aaa tgc 192 Glu Thr Ala Cys Arg Ser Leu Gly SerTyr Gln Cys Met Gly Lys Cys 50 55 60 caa ctc ggg gtt cat tcc tgg tgt gaatgc att tat aac cga ggt agt 240 Gln Leu Gly Val His Ser Trp Cys Glu CysIle Tyr Asn Arg Gly Ser 65 70 75 80 cag aag tct gga tgc gcg tgt agg tgtcaa aag tgattaattg actcatttaa 293 Gln Lys Ser Gly Cys Ala Cys Arg CysGln Lys 85 90 ctcgttgaac gatttaaaaa atccagagca atatgttcga gaaaaaccgaagacgac 350 134 91 PRT Conus lacterculatus 134 Met Met Ser Lys Met GlyAla Met Phe Val Leu Leu Leu Leu Phe Thr 1 5 10 15 Leu Ala Ser Ser GlnGln Glu Gly Asp Val Gln Ala Arg Lys Thr His 20 25 30 Pro Lys Arg Glu PheHis Arg Ile Leu Leu Arg Pro Asp Arg Gln Ser 35 40 45 Glu Thr Ala Cys ArgSer Leu Gly Ser Tyr Gln Cys Met Gly Lys Cys 50 55 60 Gln Leu Gly Val HisSer Trp Cys Glu Cys Ile Tyr Asn Arg Gly Ser 65 70 75 80 Gln Lys Ser GlyCys Ala Cys Arg Cys Gln Lys 85 90 135 45 PRT Conus lacterculatus PEPTIDE(1)..(45) Xaa at residue 1 is Gln or pyro-Gln; Xaa at residues 3 and 27is Glu or gamma-carboxy-Glu; Xaa at residue 25 is Trp (D or L) orbromo-Trp (D or L); Xaa at residues 12 and 30 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 135 Xaa SerXaa Thr Ala Cys Arg Ser Leu Gly Ser Xaa Gln Cys Met Gly 1 5 10 15 LysCys Gln Leu Gly Val His Ser Xaa Cys Xaa Cys Ile Xaa Asn Arg 20 25 30 GlySer Gln Lys Ser Gly Cys Ala Cys Arg Cys Gln Lys 35 40 45 136 318 DNAConus lividus CDS (7)..(237) 136 ggatcc atg aaa ctg acg tgt gtg gtg atcatc gcc gtg ctg ttc ctg 48 Met Lys Leu Thr Cys Val Val Ile Ile Ala ValLeu Phe Leu 1 5 10 acg gcc agt cag ctc att aca gct gat tac tcc aga gataag cag gag 96 Thr Ala Ser Gln Leu Ile Thr Ala Asp Tyr Ser Arg Asp LysGln Glu 15 20 25 30 tat cgt gca gag agg ctg aga gac gca atg ggg aaa ttcaaa ggt tcc 144 Tyr Arg Ala Glu Arg Leu Arg Asp Ala Met Gly Lys Phe LysGly Ser 35 40 45 agg tcg tgc gga cat agt ggt gca ggt tgt tat act cgc ccttgc tgc 192 Arg Ser Cys Gly His Ser Gly Ala Gly Cys Tyr Thr Arg Pro CysCys 50 55 60 cct ggt ctg cat tgc tct ggc ggc caa gct gga ggc ctg tgc gtg237 Pro Gly Leu His Cys Ser Gly Gly Gln Ala Gly Gly Leu Cys Val 65 70 75taatagtaat aatctggcgt ctgatatttc cagtctgtgc tctaccctct tttgcctgag 297tcatccatac ctgtgctcga g 318 137 77 PRT Conus lividus 137 Met Lys Leu ThrCys Val Val Ile Ile Ala Val Leu Phe Leu Thr Ala 1 5 10 15 Ser Gln LeuIle Thr Ala Asp Tyr Ser Arg Asp Lys Gln Glu Tyr Arg 20 25 30 Ala Glu ArgLeu Arg Asp Ala Met Gly Lys Phe Lys Gly Ser Arg Ser 35 40 45 Cys Gly HisSer Gly Ala Gly Cys Tyr Thr Arg Pro Cys Cys Pro Gly 50 55 60 Leu His CysSer Gly Gly Gln Ala Gly Gly Leu Cys Val 65 70 75 138 30 PRT Conuslividus PEPTIDE (1)..(30) Xaa at residues 13 and 16 is Pro orhydroxy-Pro; Xaa at residue 10 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Ty 138 Ser Cys Gly His Ser GlyAla Gly Cys Xaa Thr Arg Xaa Cys Cys Xaa 1 5 10 15 Gly Leu His Cys SerGly Gly Gln Ala Gly Gly Leu Cys Val 20 25 30 139 312 DNA Conus lividusCDS (7)..(252) 139 ggatcc atg aaa ctg acg tgt gtg gtg atc ata tcc gtgctg ttc ctg 48 Met Lys Leu Thr Cys Val Val Ile Ile Ser Val Leu Phe Leu 15 10 acg gcc agt gag ttc ctt aca gct gat tac tcc aga gat aag cgg cag 96Thr Ala Ser Glu Phe Leu Thr Ala Asp Tyr Ser Arg Asp Lys Arg Gln 15 20 2530 tac cgt gct gtg agg ttg aga gac gca atg cgg aat ttc aaa ggt acc 144Tyr Arg Ala Val Arg Leu Arg Asp Ala Met Arg Asn Phe Lys Gly Thr 35 40 45agg gac tgc ggg gaa tca ggt caa ggt tgc tat agt gta cgt cct tgc 192 ArgAsp Cys Gly Glu Ser Gly Gln Gly Cys Tyr Ser Val Arg Pro Cys 50 55 60 tgccct ggt ctg att tgc aaa ggc acc ggt ggt gga ggc ctg tgc cgg 240 Cys ProGly Leu Ile Cys Lys Gly Thr Gly Gly Gly Gly Leu Cys Arg 65 70 75 ccc tctggc atc tgatatctcc cctctgtgct ccaccctctt ttgcctgagt 292 Pro Ser Gly Ile80 catccatacc tgtgctcgag 312 140 82 PRT Conus lividus 140 Met Lys LeuThr Cys Val Val Ile Ile Ser Val Leu Phe Leu Thr Ala 1 5 10 15 Ser GluPhe Leu Thr Ala Asp Tyr Ser Arg Asp Lys Arg Gln Tyr Arg 20 25 30 Ala ValArg Leu Arg Asp Ala Met Arg Asn Phe Lys Gly Thr Arg Asp 35 40 45 Cys GlyGlu Ser Gly Gln Gly Cys Tyr Ser Val Arg Pro Cys Cys Pro 50 55 60 Gly LeuIle Cys Lys Gly Thr Gly Gly Gly Gly Leu Cys Arg Pro Ser 65 70 75 80 GlyIle 141 35 PRT Conus lividus PEPTIDE (1)..(35) Xaa at residue 4 is Gluor gamma-carboxy-Glu; Xaa at residues 14, 17 and 32 is Pro orhydroxy-Pro; Xaa at residue 10 is Tyr, 125I- Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 141 Asp Cys Gly Xaa Ser GlyGln Gly Cys Xaa Ser Val Arg Xaa Cys Cys 1 5 10 15 Xaa Gly Leu Ile CysLys Gly Thr Gly Gly Gly Gly Leu Cys Arg Xaa 20 25 30 Ser Gly Ile 35 1426 PRT Conus magus PEPTIDE (1)..(6) Xaa at residue 3 is Glu orgamma-carboxy-Glu; Xaa at residue 6 is Pro or hydroxy-Pro; Xaa atresidue 2 is Tyr, 125I-Tyr, mono- iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 142 Val Xaa Xaa Thr His Xaa 1 5 143 6 PRT Conus magusPEPTIDE (1)..(6) Xaa at residue 3 is Glu or gamma-carboxy-Glu; Xaa atresidue 6 is Pro or hydroxy-Pro; Xaa at residue 2 is Tyr, 125I-Tyr,mono- iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 143 Arg XaaLys Asn Ser Xaa 1 5 144 7 PRT Conus magus PEPTIDE (1)..(7) Xaa atresidue 2 is Pro or hydroxy-Pro; Xaa at residue 6 is Trp (D or L) orbromo-Trp (D or L) 144 Ala Arg Xaa Lys Asn Ser Xaa 1 5 145 6 PRT Conusmagus PEPTIDE (1)..(6) Xaa at residue 3 is Pro or hydroxy-Pro; Xaa atresidue 7 is Trp (D or L) or bromo-Trp (D or L) 145 Arg Xaa Lys Asn SerXaa 1 5 146 360 DNA Conus miles CDS (7)..(270) 146 ggatcc atg aaa ctgacg tgc gtg gtg atc gtc gcc gtg ctg ttc ctg 48 Met Lys Leu Thr Cys ValVal Ile Val Ala Val Leu Phe Leu 1 5 10 acg gcc tgt caa ctc att act gctgcg aat tac gcc aga gat gaa cag 96 Thr Ala Cys Gln Leu Ile Thr Ala AlaAsn Tyr Ala Arg Asp Glu Gln 15 20 25 30 gag tac ccc gct gtg agg tcg agcgac gtg atg cag gat tcc gaa gac 144 Glu Tyr Pro Ala Val Arg Ser Ser AspVal Met Gln Asp Ser Glu Asp 35 40 45 ttg acg ttg acc aag aaa tgc acg gacgat tct cag ttc tgt aac cct 192 Leu Thr Leu Thr Lys Lys Cys Thr Asp AspSer Gln Phe Cys Asn Pro 50 55 60 tcg aat cat gac tgc tgc agt ggg aag tgtatc gac gaa gga gac aac 240 Ser Asn His Asp Cys Cys Ser Gly Lys Cys IleAsp Glu Gly Asp Asn 65 70 75 ggc ata tgc gct ata gtc cct gaa aac tcttaacaatgta tactgacatt 290 Gly Ile Cys Ala Ile Val Pro Glu Asn Ser 80 85tccccctctg tgctccgccg tccgtggcct gactcgtcca tccttgggcg tggtcatgaa 350ccgctcggtt 360 147 88 PRT Conus miles 147 Met Lys Leu Thr Cys Val ValIle Val Ala Val Leu Phe Leu Thr Ala 1 5 10 15 Cys Gln Leu Ile Thr AlaAla Asn Tyr Ala Arg Asp Glu Gln Glu Tyr 20 25 30 Pro Ala Val Arg Ser SerAsp Val Met Gln Asp Ser Glu Asp Leu Thr 35 40 45 Leu Thr Lys Lys Cys ThrAsp Asp Ser Gln Phe Cys Asn Pro Ser Asn 50 55 60 His Asp Cys Cys Ser GlyLys Cys Ile Asp Glu Gly Asp Asn Gly Ile 65 70 75 80 Cys Ala Ile Val ProGlu Asn Ser 85 148 36 PRT Conus miles PEPTIDE (1)..(36) Xaa at residues23 and 34 is Glu or gamma-carboxy-Glu; Xaa at residues 10 and 33 is Proor hydroxy-Pro 148 Cys Thr Asp Asp Ser Gln Phe Cys Asn Xaa Ser Asn HisAsp Cys Cys 1 5 10 15 Ser Gly Lys Cys Ile Asp Xaa Gly Asp Asn Gly IleCys Ala Ile Val 20 25 30 Xaa Xaa Asn Ser 35 149 357 DNA Conus miles CDS(7)..(270) 149 ggatcc atg aaa ctg acg tgt gtg gtg atc gtc gcc gtg ctgttc ctg 48 Met Lys Leu Thr Cys Val Val Ile Val Ala Val Leu Phe Leu 1 510 acg gcc tgt caa ctc att act gct gcg aat tac gcc aga gat gaa cag 96Thr Ala Cys Gln Leu Ile Thr Ala Ala Asn Tyr Ala Arg Asp Glu Gln 15 20 2530 gag tac cct gct gtg agg tcg agc gac gtg atg cag gat tcc gaa gac 144Glu Tyr Pro Ala Val Arg Ser Ser Asp Val Met Gln Asp Ser Glu Asp 35 40 45ctg acg ttg acc aag aaa tgc acg gag gat tct cag ttc tgt aac cct 192 LeuThr Leu Thr Lys Lys Cys Thr Glu Asp Ser Gln Phe Cys Asn Pro 50 55 60 tcgaat cat gac tgc tgc agt ggg aag tgt atc gac gaa gga gac aac 240 Ser AsnHis Asp Cys Cys Ser Gly Lys Cys Ile Asp Glu Gly Asp Asn 65 70 75 ggc atatgc gct ata gtc cct gaa aac tct taacaatgta tactgacatt 290 Gly Ile CysAla Ile Val Pro Glu Asn Ser 80 85 tccccctctg tgctccgccg tccgtggcctgactcgtcca tccttgggcg tggtcatgaa 350 ccgctcg 357 150 88 PRT Conus miles150 Met Lys Leu Thr Cys Val Val Ile Val Ala Val Leu Phe Leu Thr Ala 1 510 15 Cys Gln Leu Ile Thr Ala Ala Asn Tyr Ala Arg Asp Glu Gln Glu Tyr 2025 30 Pro Ala Val Arg Ser Ser Asp Val Met Gln Asp Ser Glu Asp Leu Thr 3540 45 Leu Thr Lys Lys Cys Thr Glu Asp Ser Gln Phe Cys Asn Pro Ser Asn 5055 60 His Asp Cys Cys Ser Gly Lys Cys Ile Asp Glu Gly Asp Asn Gly Ile 6570 75 80 Cys Ala Ile Val Pro Glu Asn Ser 85 151 36 PRT Conus milesPEPTIDE (1)..(36) Xaa at residues 3, 23 and 34 is Glu orgamma-carboxy-Glu; Xaa at residues 10 and 33 is Pro or hydroxy-Pro 151Cys Thr Xaa Asp Ser Gln Phe Cys Asn Xaa Ser Asn His Asp Cys Cys 1 5 1015 Ser Gly Lys Cys Ile Asp Xaa Gly Asp Asn Gly Ile Cys Ala Ile Val 20 2530 Xaa Xaa Asn Ser 35 152 327 DNA Conus miliaris CDS (12)..(239) 152ggatccatga a ctg acg tgt gtg gtg atc atc gcc gtg ctg ttc ctg acg 50 LeuThr Cys Val Val Ile Ile Ala Val Leu Phe Leu Thr 1 5 10 gcc tgt caa ctcact aca gct gtg act tcc tcc aga ggt caa cag aag 98 Ala Cys Gln Leu ThrThr Ala Val Thr Ser Ser Arg Gly Gln Gln Lys 15 20 25 cat cgt gct ctg aggtca act gac aaa aac tcc agg atg acc aag cgt 146 His Arg Ala Leu Arg SerThr Asp Lys Asn Ser Arg Met Thr Lys Arg 30 35 40 45 tgc acg cct cca ggtgga ctc tgt tac cat gct tat ccc tgc tgc agc 194 Cys Thr Pro Pro Gly GlyLeu Cys Tyr His Ala Tyr Pro Cys Cys Ser 50 55 60 aag act tgc aat ctc gatacc agc caa tgt gag cct agg tgg tca 239 Lys Thr Cys Asn Leu Asp Thr SerGln Cys Glu Pro Arg Trp Ser 65 70 75 tgaaccactc aataccctct cctctggaggcttcagagga actacattga aataaaaccg 299 cattgcaacg aaaaaaaaaa aaaaaaaa 327153 76 PRT Conus miliaris 153 Leu Thr Cys Val Val Ile Ile Ala Val LeuPhe Leu Thr Ala Cys Gln 1 5 10 15 Leu Thr Thr Ala Val Thr Ser Ser ArgGly Gln Gln Lys His Arg Ala 20 25 30 Leu Arg Ser Thr Asp Lys Asn Ser ArgMet Thr Lys Arg Cys Thr Pro 35 40 45 Pro Gly Gly Leu Cys Tyr His Ala TyrPro Cys Cys Ser Lys Thr Cys 50 55 60 Asn Leu Asp Thr Ser Gln Cys Glu ProArg Trp Ser 65 70 75 154 31 PRT Conus miliaris PEPTIDE (1)..(31) Xaa atresidue 27 is Glu or gamma-carboxy-Glu; Xaa at residues 3, 4, 13 and 28is Pro or hydroxy-Pro; Xaa at residue 30 is Trp (D or L) or bromo-Trp (Dor L); Xaa at residues 9 and 12 is Tyr, 125 I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 154 Cys Thr Xaa Xaa Gly GlyLeu Cys Xaa His Ala Xaa Xaa Cys Cys Ser 1 5 10 15 Lys Thr Cys Asn LeuAsp Thr Ser Gln Cys Xaa Xaa Arg Xaa Ser 20 25 30 155 193 DNA Conusmonachus CDS (28)..(162) 155 tgtgtgtgtg tggttctggg tccagca tct gat gtcagg aat gcc gca gtc cac 54 Ser Asp Val Arg Asn Ala Ala Val His 1 5 gaaaga cag aag gat ctg gtc gtt acg gcc acc acg act tgc tgt ggt 102 Glu ArgGln Lys Asp Leu Val Val Thr Ala Thr Thr Thr Cys Cys Gly 10 15 20 25 tataat ccg atg aca atg tgc cct cct tgc atg tgc act aat acc tgc 150 Tyr AsnPro Met Thr Met Cys Pro Pro Cys Met Cys Thr Asn Thr Cys 30 35 40 aaa aaaagt ggc tgatgctcca ggaccctctg aaccacgacg t 193 Lys Lys Ser Gly 45 156 45PRT Conus monachus 156 Ser Asp Val Arg Asn Ala Ala Val His Glu Arg GlnLys Asp Leu Val 1 5 10 15 Val Thr Ala Thr Thr Thr Cys Cys Gly Tyr AsnPro Met Thr Met Cys 20 25 30 Pro Pro Cys Met Cys Thr Asn Thr Cys Lys LysSer Gly 35 40 45 157 33 PRT Conus monachus PEPTIDE (1)..(33) Xaa atresidue 1 is Gln or pyro-Gln; Xaa at residues 17, 22 and 23 is Pro orhydroxy-Pro; Xaa at residue 15 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Ty 157 Xaa Lys Asp Leu Val ValThr Ala Thr Thr Thr Cys Cys Gly Xaa Asn 1 5 10 15 Xaa Met Thr Met CysXaa Xaa Cys Met Cys Thr Asn Thr Cys Lys Lys 20 25 30 Ser 158 350 DNAConus monachus CDS (1)..(282) 158 atg atg tcg aaa atg gga gct atg tttgtc ctt ttg ctt ctt ttc acc 48 Met Met Ser Lys Met Gly Ala Met Phe ValLeu Leu Leu Leu Phe Thr 1 5 10 15 ctg gca tcc agc cag cag gaa gga gatgtc cag gca agg aaa aca agc 96 Leu Ala Ser Ser Gln Gln Glu Gly Asp ValGln Ala Arg Lys Thr Ser 20 25 30 ctg aag agc gac ttc tat cgt gct ctg agaggg tat gac aga cag tgc 144 Leu Lys Ser Asp Phe Tyr Arg Ala Leu Arg GlyTyr Asp Arg Gln Cys 35 40 45 act ctt gtc aac aat tgt gac cgg aac ggt gagcgt gcg tgt aac ggt 192 Thr Leu Val Asn Asn Cys Asp Arg Asn Gly Glu ArgAla Cys Asn Gly 50 55 60 gat tgc tct tgc gag ggc cag att tgt aaa tgc ggttat aga gtc agt 240 Asp Cys Ser Cys Glu Gly Gln Ile Cys Lys Cys Gly TyrArg Val Ser 65 70 75 80 cct ggg aag tca gga tgc gcg tgt act tgt aga aatgcc aaa 282 Pro Gly Lys Ser Gly Cys Ala Cys Thr Cys Arg Asn Ala Lys 8590 tgaatcattt aactcgttga aagatttttt aaaaatccag agctatatgt tcgagaaaaa 342ccgaagac 350 159 94 PRT Conus monachus 159 Met Met Ser Lys Met Gly AlaMet Phe Val Leu Leu Leu Leu Phe Thr 1 5 10 15 Leu Ala Ser Ser Gln GlnGlu Gly Asp Val Gln Ala Arg Lys Thr Ser 20 25 30 Leu Lys Ser Asp Phe TyrArg Ala Leu Arg Gly Tyr Asp Arg Gln Cys 35 40 45 Thr Leu Val Asn Asn CysAsp Arg Asn Gly Glu Arg Ala Cys Asn Gly 50 55 60 Asp Cys Ser Cys Glu GlyGln Ile Cys Lys Cys Gly Tyr Arg Val Ser 65 70 75 80 Pro Gly Lys Ser GlyCys Ala Cys Thr Cys Arg Asn Ala Lys 85 90 160 48 PRT Conus monachusPEPTIDE (1)..(48) Xaa at residue 1 is Gln or pyro-Gln; Xaa at residues13 and 23 is Glu or gamma-carboxy-Glu; Xaa at residue 35 is Pro orhydroxy- Pro; Xaa at residue 31 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 160 Xaa Cys Thr Leu Val Asn AsnCys Asp Arg Asn Gly Xaa Arg Ala Cys 1 5 10 15 Asn Gly Asp Cys Ser CysXaa Gly Gln Ile Cys Lys Cys Gly Xaa Arg 20 25 30 Val Ser Xaa Gly Lys SerGly Cys Ala Cys Thr Cys Arg Asn Ala Lys 35 40 45 161 211 DNA Conuspennaceus CDS (1)..(192) 161 atg cgc tgt ctc cca gtc ttc gtc att ctt ctgctg ctg act gca tct 48 Met Arg Cys Leu Pro Val Phe Val Ile Leu Leu LeuLeu Thr Ala Ser 1 5 10 15 gca cct agc gtt gat gcc aaa gtt cat ctg aagacc aaa ggt gat ggg 96 Ala Pro Ser Val Asp Ala Lys Val His Leu Lys ThrLys Gly Asp Gly 20 25 30 ccc ctg tca tct ttc cga gat aat gca aag agt acccta caa aga ctt 144 Pro Leu Ser Ser Phe Arg Asp Asn Ala Lys Ser Thr LeuGln Arg Leu 35 40 45 cag gac aaa agc act tgc tgt ggc ttt aag atg tgt attcct tgt cgt 192 Gln Asp Lys Ser Thr Cys Cys Gly Phe Lys Met Cys Ile ProCys Arg 50 55 60 taaccagcat gaaggatcc 211 162 64 PRT Conus pennaceus 162Met Arg Cys Leu Pro Val Phe Val Ile Leu Leu Leu Leu Thr Ala Ser 1 5 1015 Ala Pro Ser Val Asp Ala Lys Val His Leu Lys Thr Lys Gly Asp Gly 20 2530 Pro Leu Ser Ser Phe Arg Asp Asn Ala Lys Ser Thr Leu Gln Arg Leu 35 4045 Gln Asp Lys Ser Thr Cys Cys Gly Phe Lys Met Cys Ile Pro Cys Arg 50 5560 163 13 PRT Conus pennaceus PEPTIDE (1)..(13) Xaa at residue 11 is Proor hydroxy-Pro 163 Ser Thr Cys Cys Gly Phe Lys Met Cys Ile Xaa Cys Arg 15 10 164 450 DNA Conus pennaceus CDS (1)..(234) 164 atg ttg ctt ctg ctgttt gcc ttg ggc agc ttc gtt gtg gtc cag tca 48 Met Leu Leu Leu Leu PheAla Leu Gly Ser Phe Val Val Val Gln Ser 1 5 10 15 gga cag ata aca agagat gtg gac aat ggg cag ctc gcg gac aac cgc 96 Gly Gln Ile Thr Arg AspVal Asp Asn Gly Gln Leu Ala Asp Asn Arg 20 25 30 cgt acc ctg cga tcg cagtgg aag caa gtg agt ttc ttc aag tca ctt 144 Arg Thr Leu Arg Ser Gln TrpLys Gln Val Ser Phe Phe Lys Ser Leu 35 40 45 gat aaa cga ctg act tgt aacgat cct tgc cag atg cat tcc gat tgc 192 Asp Lys Arg Leu Thr Cys Asn AspPro Cys Gln Met His Ser Asp Cys 50 55 60 ggc ata tgt gaa tgc gtg gaa aataaa tgc ata ttt ttc atg 234 Gly Ile Cys Glu Cys Val Glu Asn Lys Cys IlePhe Phe Met 65 70 75 taaacggatt gagtttgctt gtcaacacaa tgtcgcactgcagctcttct ctaccggtgg 294 gtacatcgac caaacgacgc atcttttatt tctttgtctgtttcgtttgt tctcctgtgt 354 tcataacgta cagagccctt taactaccct tactgctcttcacttaacct gataacctga 414 aggtccggtg cagctggcgt agccttcaca gtttcg 450165 78 PRT Conus pennaceus 165 Met Leu Leu Leu Leu Phe Ala Leu Gly SerPhe Val Val Val Gln Ser 1 5 10 15 Gly Gln Ile Thr Arg Asp Val Asp AsnGly Gln Leu Ala Asp Asn Arg 20 25 30 Arg Thr Leu Arg Ser Gln Trp Lys GlnVal Ser Phe Phe Lys Ser Leu 35 40 45 Asp Lys Arg Leu Thr Cys Asn Asp ProCys Gln Met His Ser Asp Cys 50 55 60 Gly Ile Cys Glu Cys Val Glu Asn LysCys Ile Phe Phe Met 65 70 75 166 27 PRT Conus pennaceus PEPTIDE(1)..(27) Xaa at residues 17 and 20 is Glu or gamma-carboxy-Glu; Xaa atresidue 6 is Pro or hydroxy-Pro 166 Leu Thr Cys Asn Asp Xaa Cys Gln MetHis Ser Asp Cys Gly Ile Cys 1 5 10 15 Xaa Cys Val Xaa Asn Lys Cys IlePhe Phe Met 20 25 167 413 DNA Conus pulicarius CDS (1)..(243) 167 atgaaa ctg acg tgt gtg gtg atc gtc gcc gtg ctg ttc ctg acg gcc 48 Met LysLeu Thr Cys Val Val Ile Val Ala Val Leu Phe Leu Thr Ala 1 5 10 15 tgtcaa ctc agt aca gct gat gac tcc aga gat gag cag cag gat cct 96 Cys GlnLeu Ser Thr Ala Asp Asp Ser Arg Asp Glu Gln Gln Asp Pro 20 25 30 ttg gtgagg tcg cat cgt gag gag cag aaa gcc gag gac ccc aag acg 144 Leu Val ArgSer His Arg Glu Glu Gln Lys Ala Glu Asp Pro Lys Thr 35 40 45 gcc gag agatgt tca gat ttc gga tcc gac tgt gtt cct gct act cat 192 Ala Glu Arg CysSer Asp Phe Gly Ser Asp Cys Val Pro Ala Thr His 50 55 60 aac tgc tgc agtggt gaa tgt ttt ggc ttc gag gac ttc ggc tta tgc 240 Asn Cys Cys Ser GlyGlu Cys Phe Gly Phe Glu Asp Phe Gly Leu Cys 65 70 75 80 acg taaaactggtctgacgtctg atattccccc ctctgtcctt catcctcttt 293 Thr tgcctgattcatccatacct atatgtgctc ctgaaccgct gtgtaccttt accctggtgg 353 cttcagaggacgttatatca aaataaaacc gcgttgcaat gacaaaaaaa aaaaaaaaaa 413 168 81 PRTConus pulicarius 168 Met Lys Leu Thr Cys Val Val Ile Val Ala Val Leu PheLeu Thr Ala 1 5 10 15 Cys Gln Leu Ser Thr Ala Asp Asp Ser Arg Asp GluGln Gln Asp Pro 20 25 30 Leu Val Arg Ser His Arg Glu Glu Gln Lys Ala GluAsp Pro Lys Thr 35 40 45 Ala Glu Arg Cys Ser Asp Phe Gly Ser Asp Cys ValPro Ala Thr His 50 55 60 Asn Cys Cys Ser Gly Glu Cys Phe Gly Phe Glu AspPhe Gly Leu Cys 65 70 75 80 Thr 169 30 PRT Conus pulicarius PEPTIDE(1)..(30) Xaa at residues 19 and 24 is Glu or gamma-carboxy-Glu; Xaa atresidue 10 is Pro or hydroxy-Pro 169 Cys Ser Asp Phe Gly Ser Asp Cys ValXaa Ala Thr His Asn Cys Cys 1 5 10 15 Ser Gly Xaa Cys Phe Gly Phe XaaAsp Phe Gly Leu Cys Thr 20 25 30 170 375 DNA Conus purpurascens CDS(24)..(260) 170 gacaggattg aacaaaattc agg atg tca aga ttt gga atc atggtg cta acc 53 Met Ser Arg Phe Gly Ile Met Val Leu Thr 1 5 10 ttt ctactt ctt gtg tcc atg gca acc agc cat cgt tat gca aga ggg 101 Phe Leu LeuLeu Val Ser Met Ala Thr Ser His Arg Tyr Ala Arg Gly 15 20 25 aag cag gcgacg cga agg aac gca atc aac atc aga cgg aga agc aca 149 Lys Gln Ala ThrArg Arg Asn Ala Ile Asn Ile Arg Arg Arg Ser Thr 30 35 40 cca aaa act gaggcg tgc gaa gag gtc tgt gag ctg gaa gaa aag cac 197 Pro Lys Thr Glu AlaCys Glu Glu Val Cys Glu Leu Glu Glu Lys His 45 50 55 tgc tgc tgc ata agaagt gac gga ccc aaa tgt tcc cgt aag tgc ctg 245 Cys Cys Cys Ile Arg SerAsp Gly Pro Lys Cys Ser Arg Lys Cys Leu 60 65 70 ttg tca atc ttc tgttagtttctgt acactgtctc attcattatc ttatcagtac 300 Leu Ser Ile Phe Cys 75aagtgtaaac gagacatgtc agaaagtcga aggttgtgcg taatttgata agtattgttt 360gctgggatga acgga 375 171 79 PRT Conus purpurascens 171 Met Ser Arg PheGly Ile Met Val Leu Thr Phe Leu Leu Leu Val Ser 1 5 10 15 Met Ala ThrSer His Arg Tyr Ala Arg Gly Lys Gln Ala Thr Arg Arg 20 25 30 Asn Ala IleAsn Ile Arg Arg Arg Ser Thr Pro Lys Thr Glu Ala Cys 35 40 45 Glu Glu ValCys Glu Leu Glu Glu Lys His Cys Cys Cys Ile Arg Ser 50 55 60 Asp Gly ProLys Cys Ser Arg Lys Cys Leu Leu Ser Ile Phe Cys 65 70 75 172 37 PRTConus purpurascens PEPTIDE (1)..(37) Xaa at residues 4, 7, 8, 11, 13 and14 is Glu or gamma-carboxy- Glu; Xaa at residues 3 and 25 is Pro orhydroxy-Pro 172 Xaa Lys Thr Xaa Ala Cys Xaa Xaa Val Cys Xaa Leu Xaa XaaLys His 1 5 10 15 Cys Cys Cys Ile Arg Ser Asp Gly Xaa Lys Cys Ser ArgLys Cys Leu 20 25 30 Leu Ser Ile Phe Cys 35 173 373 DNA Conuspurpurascens CDS (24)..(260) 173 gacaggattg aacaaaattc agg atg tca ggattg gga atc atg gtg cta acc 53 Met Ser Gly Leu Gly Ile Met Val Leu Thr 15 10 ctt cta ctt ctt gtg tcc atg gca acc aac cat cag gat aga gga gag 101Leu Leu Leu Leu Val Ser Met Ala Thr Asn His Gln Asp Arg Gly Glu 15 20 25aag cag gtg acg caa agg gac gca atc aac gtc aga cgg aga aga tca 149 LysGln Val Thr Gln Arg Asp Ala Ile Asn Val Arg Arg Arg Arg Ser 30 35 40 atcacc cag caa gtc gta tct gag gag tgc aaa aag tac tgt aag aaa 197 Ile ThrGln Gln Val Val Ser Glu Glu Cys Lys Lys Tyr Cys Lys Lys 45 50 55 cag aacaag aat tgc tgc agc agt aaa cat gaa gaa ccc aga tgt gcc 245 Gln Asn LysAsn Cys Cys Ser Ser Lys His Glu Glu Pro Arg Cys Ala 60 65 70 aag ata tgcttc gga tagtttctgt acacggtctc attcattatt ttatcagtac 300 Lys Ile Cys PheGly 75 aagttaaacg agacctatca gaagtcgaag gttgtgcata atttgataaa cattgtttgc360 tgggatgaac gga 373 174 79 PRT Conus purpurascens 174 Met Ser Gly LeuGly Ile Met Val Leu Thr Leu Leu Leu Leu Val Ser 1 5 10 15 Met Ala ThrAsn His Gln Asp Arg Gly Glu Lys Gln Val Thr Gln Arg 20 25 30 Asp Ala IleAsn Val Arg Arg Arg Arg Ser Ile Thr Gln Gln Val Val 35 40 45 Ser Glu GluCys Lys Lys Tyr Cys Lys Lys Gln Asn Lys Asn Cys Cys 50 55 60 Ser Ser LysHis Glu Glu Pro Arg Cys Ala Lys Ile Cys Phe Gly 65 70 75 175 32 PRTConus purpurascens PEPTIDE (1)..(32) Xaa at residues 4, 5, 23 and 24 isGlu or gamma-carboxy-Glu; Xaa at residue 25 is Pro or hydroxy-Pro; Xaaat residue 9 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyror O-phospho-Tyr 175 Val Val Ser Xaa Xaa Cys Lys Lys Xaa Cys Lys Lys GlnAsn Lys Asn 1 5 10 15 Cys Cys Ser Ser Lys His Xaa Xaa Xaa Arg Cys AlaLys Ile Cys Phe 20 25 30 176 24 PRT Conus purpurascens PEPTIDE (1)..(24)Xaa at residue 8 is Glu or gamma-carboxy-Glu; Xaa at residue 12 is Proor hydroxy-Pro; Xaa at residue 22 is Tyr, 125I-Tyr, mono- iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 176 Asp Cys Cys Gly Val LysLeu Xaa Met Cys His Xaa Cys Leu Cys Asp 1 5 10 15 Asn Ser Cys Lys AsnXaa Gly Lys 20 177 235 DNA Conus purpurascens CDS (1)..(204) 177 atg ttcacc gtg ttt ctg ttg gtt gtc ttg gca acc act gtc gtt tcc 48 Met Phe ThrVal Phe Leu Leu Val Val Leu Ala Thr Thr Val Val Ser 1 5 10 15 ttc acttca gat cgt gca tcg gat gac agg aat acc aac gac aaa gca 96 Phe Thr SerAsp Arg Ala Ser Asp Asp Arg Asn Thr Asn Asp Lys Ala 20 25 30 tct cgc ctgctc tct cac gtt gtc agg gga tgc tgt ggt agc tat ccc 144 Ser Arg Leu LeuSer His Val Val Arg Gly Cys Cys Gly Ser Tyr Pro 35 40 45 aat gct gcc tgtcat cct tgc ggt tgt aaa gat agg cca tcg tat tgt 192 Asn Ala Ala Cys HisPro Cys Gly Cys Lys Asp Arg Pro Ser Tyr Cys 50 55 60 ggt caa gga cgctgatgctcca ggaccctctg aaccacgacg t 235 Gly Gln Gly Arg 65 178 68 PRTConus purpurascens 178 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala ThrThr Val Val Ser 1 5 10 15 Phe Thr Ser Asp Arg Ala Ser Asp Asp Arg AsnThr Asn Asp Lys Ala 20 25 30 Ser Arg Leu Leu Ser His Val Val Arg Gly CysCys Gly Ser Tyr Pro 35 40 45 Asn Ala Ala Cys His Pro Cys Gly Cys Lys AspArg Pro Ser Tyr Cys 50 55 60 Gly Gln Gly Arg 65 179 25 PRT Conuspurpurascens PEPTIDE (1)..(25) Xaa at residues 7, 13 and 20 is Pro orhydroxy-Pro; Xaa at residues 6 and 22 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo- Tyr, O-sulpho-Tyr or O-phospho-Tyr 179 Gly Cys Cys Gly Ser XaaXaa Asn Ala Ala Cys His Xaa Cys Gly Cys 1 5 10 15 Lys Asp Arg Xaa SerXaa Cys Gly Gln 20 25 180 229 DNA Conus purpurascens CDS (1)..(198) 180atg ttc acc gtg ttt ctg ttg gtt gtc ttg gca acc act gtc gtt tcc 48 MetPhe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val Val Ser 1 5 10 15ttc acc gta gat cgt gca act gat ggc agg agt gct gca gcc ata gcg 96 PheThr Val Asp Arg Ala Thr Asp Gly Arg Ser Ala Ala Ala Ile Ala 20 25 30 tttgcc ctg atc gct ccg acc gtc cgg gaa gga tgc tgt tct aat cct 144 Phe AlaLeu Ile Ala Pro Thr Val Arg Glu Gly Cys Cys Ser Asn Pro 35 40 45 gcc tgtcat cct tgc ggt tgt aaa gat agg cca tcg tat tgt ggt caa 192 Ala Cys HisPro Cys Gly Cys Lys Asp Arg Pro Ser Tyr Cys Gly Gln 50 55 60 gga cgctgatgctcca ggaccctctg aaccacgacg t 229 Gly Arg 65 181 66 PRT Conuspurpurascens 181 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr ValVal Ser 1 5 10 15 Phe Thr Val Asp Arg Ala Thr Asp Gly Arg Ser Ala AlaAla Ile Ala 20 25 30 Phe Ala Leu Ile Ala Pro Thr Val Arg Glu Gly Cys CysSer Asn Pro 35 40 45 Ala Cys His Pro Cys Gly Cys Lys Asp Arg Pro Ser TyrCys Gly Gln 50 55 60 Gly Arg 65 182 23 PRT Conus purpurascens PEPTIDE(1)..(23) Xaa at residue 1 is Glu or gamma-carboxy-Glu; Xaa at residues7, 11 and 18 is Pro or hydroxy-Pro; Xaa at residue 20 is Tyr, 125I- Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O- phospho-Tyr 182 Xaa GlyCys Cys Ser Asn Xaa Ala Cys His Xaa Cys Gly Cys Lys Asp 1 5 10 15 ArgXaa Ser Xaa Cys Gly Gln 20 183 334 DNA Conus purpurascens CDS (1)..(261)183 atg atg tcg aaa atg gga gct atg ttt gtc ctt ttg ctt ctt ttc acc 48Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu Leu Leu Phe Thr 1 5 1015 ctg gca tcc agc cag cag gaa gga gat gtc cag gca agg aaa aca cgc 96Leu Ala Ser Ser Gln Gln Glu Gly Asp Val Gln Ala Arg Lys Thr Arg 20 25 30ctg acg agg gac ttc tat cgt act ctg cca gtg tct act aga gga tgc 144 LeuThr Arg Asp Phe Tyr Arg Thr Leu Pro Val Ser Thr Arg Gly Cys 35 40 45 agcggc tcc cct tgt ttt aaa aac aaa acg tgt cgg gat gaa tgc ata 192 Ser GlySer Pro Cys Phe Lys Asn Lys Thr Cys Arg Asp Glu Cys Ile 50 55 60 tgc ggcggc tta tcc aat tgt tgg tgt ggc tac ggc ggt agt cga gga 240 Cys Gly GlyLeu Ser Asn Cys Trp Cys Gly Tyr Gly Gly Ser Arg Gly 65 70 75 80 tgc aagtgt aca tgt aga gag tgattaatcg actctttaac tcgttgaatt 291 Cys Lys Cys ThrCys Arg Glu 85 atttaaaaaa tccagagcaa tatgttcgag aaaaaccgaa gac 334 18487 PRT Conus purpurascens 184 Met Met Ser Lys Met Gly Ala Met Phe ValLeu Leu Leu Leu Phe Thr 1 5 10 15 Leu Ala Ser Ser Gln Gln Glu Gly AspVal Gln Ala Arg Lys Thr Arg 20 25 30 Leu Thr Arg Asp Phe Tyr Arg Thr LeuPro Val Ser Thr Arg Gly Cys 35 40 45 Ser Gly Ser Pro Cys Phe Lys Asn LysThr Cys Arg Asp Glu Cys Ile 50 55 60 Cys Gly Gly Leu Ser Asn Cys Trp CysGly Tyr Gly Gly Ser Arg Gly 65 70 75 80 Cys Lys Cys Thr Cys Arg Glu 85185 41 PRT Conus purpurascens PEPTIDE (1)..(41) Xaa at residue 16 is Gluor gamma-carboxy-Glu; Xaa at residue 6 is Pro or hydroxy-Pro; Xaa atresidue 26 is Trp (D or L) or bromo-Trp (D or L); Xaa at residue 29 isTyr, 125I-Tyr, mono- iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 185 Gly Cys Ser Gly Ser Xaa Cys Phe Lys Asn Lys Thr CysArg Asp Xaa 1 5 10 15 Cys Ile Cys Gly Gly Leu Ser Asn Cys Xaa Cys GlyXaa Gly Gly Ser 20 25 30 Arg Gly Cys Lys Cys Thr Cys Arg Xaa 35 40 186327 DNA Conus purpurascens CDS (29)..(256) 186 cgacctcaag agggatcgatagcagttc atg atg tct aaa ctg gga gcc ttg 52 Met Met Ser Lys Leu Gly AlaLeu 1 5 ttg acc atc tgt ctg ctt ctg ttt ccc att act gct ctt ctg atg gat100 Leu Thr Ile Cys Leu Leu Leu Phe Pro Ile Thr Ala Leu Leu Met Asp 1015 20 gga gat caa cct gca gac cga cct gca gaa cgt atg gat tac gac att148 Gly Asp Gln Pro Ala Asp Arg Pro Ala Glu Arg Met Asp Tyr Asp Ile 2530 35 40 tca tct gag gtg cat cgt ttg ctt gaa agg aga cac ccg ccc tgt tgc196 Ser Ser Glu Val His Arg Leu Leu Glu Arg Arg His Pro Pro Cys Cys 4550 55 atg tac ggc aga tgc cgt cga tat ccc gga tgc tct agt gcc tct tgt244 Met Tyr Gly Arg Cys Arg Arg Tyr Pro Gly Cys Ser Ser Ala Ser Cys 6065 70 tgc cag gga gga taacgtgttg atgaccaact ttgttacacg gctacgtcaa 296Cys Gln Gly Gly 75 gtgtctactg aataagtaaa acgattgcag t 327 187 76 PRTConus purpurascens 187 Met Met Ser Lys Leu Gly Ala Leu Leu Thr Ile CysLeu Leu Leu Phe 1 5 10 15 Pro Ile Thr Ala Leu Leu Met Asp Gly Asp GlnPro Ala Asp Arg Pro 20 25 30 Ala Glu Arg Met Asp Tyr Asp Ile Ser Ser GluVal His Arg Leu Leu 35 40 45 Glu Arg Arg His Pro Pro Cys Cys Met Tyr GlyArg Cys Arg Arg Tyr 50 55 60 Pro Gly Cys Ser Ser Ala Ser Cys Cys Gln GlyGly 65 70 75 188 24 PRT Conus purpurascens PEPTIDE (1)..(24) Xaa atresidues 2, 3 and 14 is Pro or hydroxy-Pro; Xaa at residue residues 7and 13 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 188 His Xaa Xaa Cys Cys Met Xaa Gly Arg Cys Arg Arg XaaXaa Gly Cys 1 5 10 15 Ser Ser Ala Ser Cys Cys Gln Gly 20 189 24 PRTConus purpurascens PEPTIDE (1)..(24) Xaa at residues 2, 3, 12 and 14 isPro or hydroxy-Pro; Xaa at residues 7 and 17 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 189 Gly XaaXaa Cys Cys Leu Xaa Gly Ser Cys Arg Xaa Phe Xaa Gly Cys 1 5 10 15 XaaAsn Ala Leu Cys Cys Arg Lys 20 190 400 DNA Conus quercinus CDS(7)..(237) 190 ggatcc atg aaa ctg acg tgc gtg gtg atc atc gcc gtg ctgttt ctg 48 Met Lys Leu Thr Cys Val Val Ile Ile Ala Val Leu Phe Leu 1 510 aca gcc agt cag ctc gtt aca gct gat tac acc aga gat aaa tgg caa 96Thr Ala Ser Gln Leu Val Thr Ala Asp Tyr Thr Arg Asp Lys Trp Gln 15 20 2530 tac cct gca gcg agt ttg aga ggc gga atg tgg aat ttg aga gat acc 144Tyr Pro Ala Ala Ser Leu Arg Gly Gly Met Trp Asn Leu Arg Asp Thr 35 40 45agg gcg tgc tcg caa gta ggt gaa gct tgt ttt cct cag aaa cct tgc 192 ArgAla Cys Ser Gln Val Gly Glu Ala Cys Phe Pro Gln Lys Pro Cys 50 55 60 tgccct gga ttc ctt tgc aat cac atc gga ggc atg tgc cac cac 237 Cys Pro GlyPhe Leu Cys Asn His Ile Gly Gly Met Cys His His 65 70 75 tagtaacagtctggcatctg atatttcccc tctgcgctcc accctctttt ggctgattca 297 tccttacctgtgtgtggtca tgaaccactc agtagctaca cctctggtgg cttcagagga 357 cgtatatcaaaataaaacca cattgcaaaa aaaaaaaaaa aaa 400 191 77 PRT Conus quercinus 191Met Lys Leu Thr Cys Val Val Ile Ile Ala Val Leu Phe Leu Thr Ala 1 5 1015 Ser Gln Leu Val Thr Ala Asp Tyr Thr Arg Asp Lys Trp Gln Tyr Pro 20 2530 Ala Ala Ser Leu Arg Gly Gly Met Trp Asn Leu Arg Asp Thr Arg Ala 35 4045 Cys Ser Gln Val Gly Glu Ala Cys Phe Pro Gln Lys Pro Cys Cys Pro 50 5560 Gly Phe Leu Cys Asn His Ile Gly Gly Met Cys His His 65 70 75 192 30PRT Conus quercinus PEPTIDE (1)..(30) Xaa at residue 7 is Glu orgamma-carboxy-Glu; Xaa at residues 11, 14 and 17 is Pro or hydroxy-Pro192 Ala Cys Ser Gln Val Gly Xaa Ala Cys Phe Xaa Gln Lys Xaa Cys Cys 1 510 15 Xaa Gly Phe Leu Cys Asn His Ile Gly Gly Met Cys His His 20 25 30193 11 PRT Conus quercinus PEPTIDE (1)..(11) Xaa at residue 4 is Pro orhydroxy-Pro 193 Asp Cys Gln Xaa Cys Gly His Asn Val Cys Cys 1 5 10 194336 DNA Conus radiatus CDS (2)..(241) 194 c atg aac tgt ctc gta ctg gctttg gtt acc atc ggt ctt ctg gct gca 49 Met Asn Cys Leu Val Leu Ala LeuVal Thr Ile Gly Leu Leu Ala Ala 1 5 10 15 aca acc gca gcc cct ctg gacacc acc acg gtc ctc ctc agc aca act 97 Thr Thr Ala Ala Pro Leu Asp ThrThr Thr Val Leu Leu Ser Thr Thr 20 25 30 aca cgc gat gtc aag ggc tgt gtgtac gag ggc ata gag tac agt gtc 145 Thr Arg Asp Val Lys Gly Cys Val TyrGlu Gly Ile Glu Tyr Ser Val 35 40 45 gga gag acc tac cag gca gac tgc aacacg tgt cgc tgt gat ggc ttt 193 Gly Glu Thr Tyr Gln Ala Asp Cys Asn ThrCys Arg Cys Asp Gly Phe 50 55 60 gac ctg gct aca tgc acc gtc gcg ggc tgcaca ggc ttt gga ccc gag 241 Asp Leu Ala Thr Cys Thr Val Ala Gly Cys ThrGly Phe Gly Pro Glu 65 70 75 80 tgattggtac tattccacac ctagcaatgttcacactgga accggaactt gatactacct 301 tctaaatata atcaatttgt ttcaaaaggcccaaa 336 195 80 PRT Conus radiatus 195 Met Asn Cys Leu Val Leu Ala LeuVal Thr Ile Gly Leu Leu Ala Ala 1 5 10 15 Thr Thr Ala Ala Pro Leu AspThr Thr Thr Val Leu Leu Ser Thr Thr 20 25 30 Thr Arg Asp Val Lys Gly CysVal Tyr Glu Gly Ile Glu Tyr Ser Val 35 40 45 Gly Glu Thr Tyr Gln Ala AspCys Asn Thr Cys Arg Cys Asp Gly Phe 50 55 60 Asp Leu Ala Thr Cys Thr ValAla Gly Cys Thr Gly Phe Gly Pro Glu 65 70 75 80 196 43 PRT Conusradiatus PEPTIDE (1)..(43) Xaa at residues 5, 8, 13 and 43 is Glu orgamma-carboxy-Glu; Xaa at residue 42 is Pro or hydroxy-Pro; Xaa atresidues 4,9 and 15 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr,O-sulpho-Tyr or O-phospho-Tyr 196 Gly Cys Val Xaa Xaa Gly Ile Xaa XaaSer Val Gly Xaa Thr Xaa Gln 1 5 10 15 Ala Asp Cys Asn Thr Cys Arg CysAsp Gly Phe Asp Leu Ala Thr Cys 20 25 30 Thr Val Ala Gly Cys Thr Gly PheGly Xaa Xaa 35 40 197 536 DNA Conus radiatus CDS (87)..(296) 197gtgagagtcc aacagcccaa acctttcaac tcactatgtg gcagttgcag ttttcaacgt 60ctggacagga ttcaacaaaa ttcagg atg tca gga ttg gga atc atg gtg cta 113 MetSer Gly Leu Gly Ile Met Val Leu 1 5 acc ctt cta ctt ctt gtg tcc atg gcaacc agt cgt cag gat aga gga 161 Thr Leu Leu Leu Leu Val Ser Met Ala ThrSer Arg Gln Asp Arg Gly 10 15 20 25 gtg gga cag ctg atg cca cgc gtc tcgttc aaa gcc tgc aaa tca aat 209 Val Gly Gln Leu Met Pro Arg Val Ser PheLys Ala Cys Lys Ser Asn 30 35 40 tat gat tgc ccc cag cgt ttc aaa tgc tgcagt tac acc tgg aat gga 257 Tyr Asp Cys Pro Gln Arg Phe Lys Cys Cys SerTyr Thr Trp Asn Gly 45 50 55 tcc agt gga tac tgt aaa cgt gtt tgc tat ctttat cgt tagtgtaata 306 Ser Ser Gly Tyr Cys Lys Arg Val Cys Tyr Leu TyrArg 60 65 70 cacaaagtga ctctgttcat tcctctccat catctcttta gaaacaacacggtgtcgaga 366 tcgtttcttt gtgatgaaga gtagtatcac gggcagagtt cactagagatctcaaatgaa 426 aaacaagatt atttagtaag ttggggaaaa tctggatctc gaaaagattccttgaaaact 486 ccgtatttaa cacgcttgag agatgataat aaagaattct gaaagacaaa536 198 70 PRT Conus radiatus 198 Met Ser Gly Leu Gly Ile Met Val LeuThr Leu Leu Leu Leu Val Ser 1 5 10 15 Met Ala Thr Ser Arg Gln Asp ArgGly Val Gly Gln Leu Met Pro Arg 20 25 30 Val Ser Phe Lys Ala Cys Lys SerAsn Tyr Asp Cys Pro Gln Arg Phe 35 40 45 Lys Cys Cys Ser Tyr Thr Trp AsnGly Ser Ser Gly Tyr Cys Lys Arg 50 55 60 Val Cys Tyr Leu Tyr Arg 65 70199 34 PRT Conus radiatus PEPTIDE (1)..(34) Xaa at residue 9 is Pro orhydroxy-Pro; Xaa at residue 19 is Trp (D or L) or bromo-Trp (D or L);Xaa at residues 6, 17 and 33 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 199 Ala Cys Lys Ser Asn XaaAsp Cys Xaa Gln Arg Phe Lys Cys Cys Ser 1 5 10 15 Xaa Thr Xaa Asn GlySer Ser Gly Xaa Cys Lys Arg Val Cys Xaa Leu 20 25 30 Xaa Arg 200 356 DNAConus radiatus CDS (1)..(279) 200 atg atg tcg aaa atg gga gct atg tttgtc ctt ttg ctt ctt ttc acc 48 Met Met Ser Lys Met Gly Ala Met Phe ValLeu Leu Leu Leu Phe Thr 1 5 10 15 ctg gca tcc agc cag cag gaa gga gatgtc cag gca agg aaa aca cac 96 Leu Ala Ser Ser Gln Gln Glu Gly Asp ValGln Ala Arg Lys Thr His 20 25 30 ccg aag aga gag ttc caa cgt att ctg ctaagg tct ggc aga aag tgc 144 Pro Lys Arg Glu Phe Gln Arg Ile Leu Leu ArgSer Gly Arg Lys Cys 35 40 45 aat ttc gac aaa tgt aaa ggt acc gga gtc tacaat tgt ggg gaa tcc 192 Asn Phe Asp Lys Cys Lys Gly Thr Gly Val Tyr AsnCys Gly Glu Ser 50 55 60 tgc tca tgc gaa ggt ttg cac agt tgt cgc tgc acttat aac atc ggt 240 Cys Ser Cys Glu Gly Leu His Ser Cys Arg Cys Thr TyrAsn Ile Gly 65 70 75 80 tct atg aag tct gga tgc gcg tgt att tgt aca tactat taatgattaa 289 Ser Met Lys Ser Gly Cys Ala Cys Ile Cys Thr Tyr Tyr85 90 ttgactcgtt taactcgttg aacgatttaa aaaatccaga gcaatatgtt cgagaaaaac349 cgaagac 356 201 93 PRT Conus radiatus 201 Met Met Ser Lys Met GlyAla Met Phe Val Leu Leu Leu Leu Phe Thr 1 5 10 15 Leu Ala Ser Ser GlnGln Glu Gly Asp Val Gln Ala Arg Lys Thr His 20 25 30 Pro Lys Arg Glu PheGln Arg Ile Leu Leu Arg Ser Gly Arg Lys Cys 35 40 45 Asn Phe Asp Lys CysLys Gly Thr Gly Val Tyr Asn Cys Gly Glu Ser 50 55 60 Cys Ser Cys Glu GlyLeu His Ser Cys Arg Cys Thr Tyr Asn Ile Gly 65 70 75 80 Ser Met Lys SerGly Cys Ala Cys Ile Cys Thr Tyr Tyr 85 90 202 47 PRT Conus radiatusPEPTIDE (1)..(47) Xaa at residues 17 and 22 is Glu or gamma-carboxy-Glu;Xaa at residues 13, 31, 46 and 47 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 202 Lys Cys Asn Phe Asp Lys CysLys Gly Thr Gly Val Xaa Asn Cys Gly 1 5 10 15 Xaa Ser Cys Ser Cys XaaGly Leu His Ser Cys Arg Cys Thr Xaa Asn 20 25 30 Ile Gly Ser Met Lys SerGly Cys Ala Cys Ile Cys Thr Xaa Xaa 35 40 45 203 338 DNA Conus radiatusCDS (1)..(264) 203 atg atg tcg aaa atg gga gct atg ttt gtc ctt ttg cttctt ttc acc 48 Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu Leu LeuPhe Thr 1 5 10 15 ctg gca tcc agg cag cag gaa gga gat gtc cag gca aggaaa aca cgc 96 Leu Ala Ser Arg Gln Gln Glu Gly Asp Val Gln Ala Arg LysThr Arg 20 25 30 ctg acg agc gac ttc tat agt gtt ctg caa agg tat gga ctagga tgc 144 Leu Thr Ser Asp Phe Tyr Ser Val Leu Gln Arg Tyr Gly Leu GlyCys 35 40 45 gct ggc act tgt ggt tca agc agc aat tgt gtt aga gat tat tgtgac 192 Ala Gly Thr Cys Gly Ser Ser Ser Asn Cys Val Arg Asp Tyr Cys Asp50 55 60 tgc cca aaa ccc aat tgt tac tgc act ggc aaa ggc ttt cgt caa cca240 Cys Pro Lys Pro Asn Cys Tyr Cys Thr Gly Lys Gly Phe Arg Gln Pro 6570 75 80 gga tgc ggg tgt tca tgt ttg ggg tgattaattg gctcttttaactcgttgaac 294 Gly Cys Gly Cys Ser Cys Leu Gly 85 gatttaaaaa atccagagcaatatgttcga gaaaaaccga agac 338 204 88 PRT Conus radiatus 204 Met Met SerLys Met Gly Ala Met Phe Val Leu Leu Leu Leu Phe Thr 1 5 10 15 Leu AlaSer Arg Gln Gln Glu Gly Asp Val Gln Ala Arg Lys Thr Arg 20 25 30 Leu ThrSer Asp Phe Tyr Ser Val Leu Gln Arg Tyr Gly Leu Gly Cys 35 40 45 Ala GlyThr Cys Gly Ser Ser Ser Asn Cys Val Arg Asp Tyr Cys Asp 50 55 60 Cys ProLys Pro Asn Cys Tyr Cys Thr Gly Lys Gly Phe Arg Gln Pro 65 70 75 80 GlyCys Gly Cys Ser Cys Leu Gly 85 205 44 PRT Conus radiatus PEPTIDE(1)..(44) Xaa at residues 23, 25 and 37 is Pro or hydroxy-Pro; Xaa atresidues 1, 19 and 28 is Tyr, 125I-Tyr, mono-iodo-Tyr, di- iodo-Tyr,O-sulpho-Tyr or O-phospho-Tyr 205 Xaa Gly Leu Gly Cys Ala Gly Thr CysGly Ser Ser Ser Asn Cys Val 1 5 10 15 Arg Asp Xaa Cys Asp Cys Xaa LysXaa Asn Cys Xaa Cys Thr Gly Lys 20 25 30 Gly Phe Arg Gln Xaa Gly Cys GlyCys Ser Cys Leu 35 40 206 375 DNA Conus sponsalis CDS (24)..(260) 206gacaggattg aacaaaattc agg atg tca gga ttg gga atc atg gtg ctg acc 53 MetSer Gly Leu Gly Ile Met Val Leu Thr 1 5 10 ctt ttg ctt ctt gtg tcc atggca acc agc cat aag gat gga gga gag 101 Leu Leu Leu Leu Val Ser Met AlaThr Ser His Lys Asp Gly Gly Glu 15 20 25 aag cag gcg atg caa agg gac gcaatc aac gtc aga ctg aga aga tca 149 Lys Gln Ala Met Gln Arg Asp Ala IleAsn Val Arg Leu Arg Arg Ser 30 35 40 ctc act cgg aga gca gta act gag gcgtgc acg gag gac tgt aag act 197 Leu Thr Arg Arg Ala Val Thr Glu Ala CysThr Glu Asp Cys Lys Thr 45 50 55 cag gac aag aag tgc tgc ggc gaa atg aatgga caa cac aca tgt gcc 245 Gln Asp Lys Lys Cys Cys Gly Glu Met Asn GlyGln His Thr Cys Ala 60 65 70 aag ata tgc ctc gga tagtctctgt acgctgtctcattcattatc tcatcagtac 300 Lys Ile Cys Leu Gly 75 aagtgtaaac gagacaggtcagaaagtcga aggttgttcg aaatttgata agcattgttt 360 actgggacga acgga 375 20779 PRT Conus sponsalis 207 Met Ser Gly Leu Gly Ile Met Val Leu Thr LeuLeu Leu Leu Val Ser 1 5 10 15 Met Ala Thr Ser His Lys Asp Gly Gly GluLys Gln Ala Met Gln Arg 20 25 30 Asp Ala Ile Asn Val Arg Leu Arg Arg SerLeu Thr Arg Arg Ala Val 35 40 45 Thr Glu Ala Cys Thr Glu Asp Cys Lys ThrGln Asp Lys Lys Cys Cys 50 55 60 Gly Glu Met Asn Gly Gln His Thr Cys AlaLys Ile Cys Leu Gly 65 70 75 208 32 PRT Conus sponsalis PEPTIDE(1)..(32) Xaa at residues 4, 8 and 20 is Glu or gamma-carboxy-Glu 208Ala Val Thr Xaa Ala Cys Thr Xaa Asp Cys Lys Thr Gln Asp Lys Lys 1 5 1015 Cys Cys Gly Xaa Met Asn Gly Gln His Thr Cys Ala Lys Ile Cys Leu 20 2530 209 8 PRT Conus stercusmuscarum PEPTIDE (1)..(8) Xaa at residues 3and 6 is Pro or hydroxy-Pro; Xaa at residue 4 is D-Trp 209 Gly Cys XaaXaa Gln Xaa Val Cys 1 5 210 9 PRT Conus striatus PEPTIDE (1)..(9) Xaa atresidue 7 is Pro or hydroxy-Pro 210 Cys Ile Ile Arg Asn Cys Xaa Arg Gly1 5 211 238 DNA Conus striatus CDS (4)..(96) 211 agg tcg act cgc tgc ttgcct gac gga acg tct tgc ctt ttt agt agg 48 Ser Thr Arg Cys Leu Pro AspGly Thr Ser Cys Leu Phe Ser Arg 1 5 10 15 atc aga tgc tgc ggt act tgcagt tca atc tta aag tca tgt gtg agc 96 Ile Arg Cys Cys Gly Thr Cys SerSer Ile Leu Lys Ser Cys Val Ser 20 25 30 tgatccagcg gttgatcttcctccctctgt gctccatcct tttctgcctg agttctcctt 156 acctgagagt ggtcatgaaccactcatcac ctactcttct ggaggcttca gaggagctac 216 agtgaaataa aagccgcatt gc238 212 31 PRT Conus striatus 212 Ser Thr Arg Cys Leu Pro Asp Gly ThrSer Cys Leu Phe Ser Arg Ile 1 5 10 15 Arg Cys Cys Gly Thr Cys Ser SerIle Leu Lys Ser Cys Val Ser 20 25 30 213 28 PRT Conus striatus PEPTIDE(1)..(28) Xaa at residue 3 is Pro or hydroxy-Pro 213 Cys Leu Xaa Asp GlyThr Ser Cys Leu Phe Ser Arg Ile Arg Cys Cys 1 5 10 15 Gly Thr Cys SerSer Ile Leu Lys Ser Cys Val Ser 20 25 214 707 DNA Conus striatus CDS(171)..(539) 214 cggcttctaa tacgactcac tatagggcaa gcagtggtaa caacgcagagtacgcggggg 60 gacggcagac cagctgggga ccagacagac gtcaaacagc atcgcagtcaggtgtggaga 120 tcccaagaca cccagaagaa ggagacagaa gagttatcgt tcgtaacacaatg gcc 176 Met Ala 1 atg aac atg tcg atg aca ctc tgc atg ttt gta atggtc gtc gtg gca 224 Met Asn Met Ser Met Thr Leu Cys Met Phe Val Met ValVal Val Ala 5 10 15 gcc act gtc att gat tcc act cag tta caa gaa cca gatctc agt cgc 272 Ala Thr Val Ile Asp Ser Thr Gln Leu Gln Glu Pro Asp LeuSer Arg 20 25 30 atg cga cgc agc ggg cct gct gac tgt tgc agg atg aaa gagtgt tgc 320 Met Arg Arg Ser Gly Pro Ala Asp Cys Cys Arg Met Lys Glu CysCys 35 40 45 50 acc gac aga gtg aac gag tgt cta cag cgc tat tct ggc cgggaa gat 368 Thr Asp Arg Val Asn Glu Cys Leu Gln Arg Tyr Ser Gly Arg GluAsp 55 60 65 aaa ttc gtt tcg ttt tgt tat cag gag gcc aca gtc aca tgt ggatct 416 Lys Phe Val Ser Phe Cys Tyr Gln Glu Ala Thr Val Thr Cys Gly Ser70 75 80 ttt aac gaa atc gtg ggc tgt tgc tat gga tat caa atg tgc atg ata464 Phe Asn Glu Ile Val Gly Cys Cys Tyr Gly Tyr Gln Met Cys Met Ile 8590 95 cga gtt gtg aaa ccg aac agt cta agt ggg gcc cat gag gcg tgc aaa512 Arg Val Val Lys Pro Asn Ser Leu Ser Gly Ala His Glu Ala Cys Lys 100105 110 acc gtt tct tgt ggt aac cct tgc gct tgaggtgtcc tcgcgccacg 559Thr Val Ser Cys Gly Asn Pro Cys Ala 115 120 tcacctgtgt acagcgccgtcaccagagcc ctgatcttta tgcccttatc tgtctttttg 619 ctctttcact ctctgaagtcttgaggtttg ttccattctt gtcaatcatc tcacgcgcat 679 ccaagtaaat aaaggtgacgtgacaaac 707 215 123 PRT Conus striatus 215 Met Ala Met Asn Met Ser MetThr Leu Cys Met Phe Val Met Val Val 1 5 10 15 Val Ala Ala Thr Val IleAsp Ser Thr Gln Leu Gln Glu Pro Asp Leu 20 25 30 Ser Arg Met Arg Arg SerGly Pro Ala Asp Cys Cys Arg Met Lys Glu 35 40 45 Cys Cys Thr Asp Arg ValAsn Glu Cys Leu Gln Arg Tyr Ser Gly Arg 50 55 60 Glu Asp Lys Phe Val SerPhe Cys Tyr Gln Glu Ala Thr Val Thr Cys 65 70 75 80 Gly Ser Phe Asn GluIle Val Gly Cys Cys Tyr Gly Tyr Gln Met Cys 85 90 95 Met Ile Arg Val ValLys Pro Asn Ser Leu Ser Gly Ala His Glu Ala 100 105 110 Cys Lys Thr ValSer Cys Gly Asn Pro Cys Ala 115 120 216 86 PRT Conus striatus PEPTIDE(1)..(86) Xaa at residues 11, 19, 28, 38, 48 and 74 is Glu or gamma-carboxy-Glu; Xaa at residues 3, 66 and 84 is Pro or hydroxy-Pro; Xaa atresidues 24, 26, 54 and 56 is Tyr, 125I-Tyr, mono-iodo- Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 216 Ser Gly Xaa Ala Asp CysCys Arg Met Lys Xaa Cys Cys Thr Asp Arg 1 5 10 15 Val Asn Xaa Cys LeuGln Arg Xaa Ser Gly Arg Xaa Asp Lys Phe Val 20 25 30 Ser Phe Cys Xaa GlnXaa Ala Thr Val Thr Cys Gly Ser Phe Asn Xaa 35 40 45 Ile Val Gly Cys CysXaa Gly Xaa Gln Met Cys Met Ile Arg Val Val 50 55 60 Lys Xaa Asn Ser LeuSer Gly Ala His Xaa Ala Cys Lys Thr Val Ser 65 70 75 80 Cys Gly Asn XaaCys Ala 85 217 14 PRT Conus striatus PEPTIDE (1)..(14) Xaa at residues 1and 3 is Trp (D or L) or bromo-Trp (D or L) 217 Xaa Ser Xaa Arg Met GlyAsn Gly Asp Arg Arg Ser Asp Gln 1 5 10 218 343 DNA Conus striolatus CDS(1)..(276) 218 atg atg tcg aaa atg gga gct atg ttt gtc ctt ttg ctt cttttg acc 48 Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu Leu Leu LeuThr 1 5 10 15 ctg gca tcc agc cag cag gag gga gat gtc cag gca agg aaaaca agc 96 Leu Ala Ser Ser Gln Gln Glu Gly Asp Val Gln Ala Arg Lys ThrSer 20 25 30 ctg aag agc gac ttc tat cgt gct ctg aga ccg tat gac aga cagtgc 144 Leu Lys Ser Asp Phe Tyr Arg Ala Leu Arg Pro Tyr Asp Arg Gln Cys35 40 45 act ttt gtc aac aat tgt caa cag aac ggt gcg tgt aac ggt gat tgc192 Thr Phe Val Asn Asn Cys Gln Gln Asn Gly Ala Cys Asn Gly Asp Cys 5055 60 tct tgc ggg gac cag att tgt aaa tgc ggt tat aga atc agt cct ggg240 Ser Cys Gly Asp Gln Ile Cys Lys Cys Gly Tyr Arg Ile Ser Pro Gly 6570 75 80 agg tca gga tgc gcg tgt act tgt aga aat gcc aaa tgaatcactt 286Arg Ser Gly Cys Ala Cys Thr Cys Arg Asn Ala Lys 85 90 aactcgttgaaagattttta aaaatccaga gctatatgtt cgagaaaaac cgaagac 343 219 92 PRT Conusstriolatus 219 Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu Leu LeuLeu Thr 1 5 10 15 Leu Ala Ser Ser Gln Gln Glu Gly Asp Val Gln Ala ArgLys Thr Ser 20 25 30 Leu Lys Ser Asp Phe Tyr Arg Ala Leu Arg Pro Tyr AspArg Gln Cys 35 40 45 Thr Phe Val Asn Asn Cys Gln Gln Asn Gly Ala Cys AsnGly Asp Cys 50 55 60 Ser Cys Gly Asp Gln Ile Cys Lys Cys Gly Tyr Arg IleSer Pro Gly 65 70 75 80 Arg Ser Gly Cys Ala Cys Thr Cys Arg Asn Ala Lys85 90 220 46 PRT Conus striolatus PEPTIDE (1)..(46) Xaa at residue 1 isGln or pyro-Gln; Xaa at residue 33 is Pro or hydroxy-Pro; Xaa at residue29 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 220 Xaa Cys Thr Phe Val Asn Asn Cys Gln Gln Asn Gly AlaCys Asn Gly 1 5 10 15 Asp Cys Ser Cys Gly Asp Gln Ile Cys Lys Cys GlyXaa Arg Ile Ser 20 25 30 Xaa Gly Arg Ser Gly Cys Ala Cys Thr Cys Arg AsnAla Lys 35 40 45 221 398 DNA Conus tessulatus CDS (7)..(240) 221 ggatccatg aaa ctg acg tgt gtg gtg atc atc gcc gtg ctg ttc ctg 48 Met Lys LeuThr Cys Val Val Ile Ile Ala Val Leu Phe Leu 1 5 10 acg gcc tgt caa ttcatt ata gct gat ttc tcc aga gat aag cgg gta 96 Thr Ala Cys Gln Phe IleIle Ala Asp Phe Ser Arg Asp Lys Arg Val 15 20 25 30 cat cgt gca gag aggttg aga gac ata atg cag aat ttc aga ggt acc 144 His Arg Ala Glu Arg LeuArg Asp Ile Met Gln Asn Phe Arg Gly Thr 35 40 45 agg tcg tgc gcg gaa tttggt gaa gtt tgt agt tct acc gct tgc tgc 192 Arg Ser Cys Ala Glu Phe GlyGlu Val Cys Ser Ser Thr Ala Cys Cys 50 55 60 cct gat ttg gat tgc gtt gaggcc tat tca ccc atc tgt ctc tgg gaa 240 Pro Asp Leu Asp Cys Val Glu AlaTyr Ser Pro Ile Cys Leu Trp Glu 65 70 75 tagtctggca tctgatatttcccgtctgtg ctctacctac ttctgccgga ttcatccata 300 cctatgtgtg gccatgaaccactcagtacc tacacctctg gtggcttcct agggacgtat 360 atcaaaataa aaccacattgcaaaaaaaaa aaaaaaaa 398 222 78 PRT Conus tessulatus 222 Met Lys Leu ThrCys Val Val Ile Ile Ala Val Leu Phe Leu Thr Ala 1 5 10 15 Cys Gln PheIle Ile Ala Asp Phe Ser Arg Asp Lys Arg Val His Arg 20 25 30 Ala Glu ArgLeu Arg Asp Ile Met Gln Asn Phe Arg Gly Thr Arg Ser 35 40 45 Cys Ala GluPhe Gly Glu Val Cys Ser Ser Thr Ala Cys Cys Pro Asp 50 55 60 Leu Asp CysVal Glu Ala Tyr Ser Pro Ile Cys Leu Trp Glu 65 70 75 223 31 PRT Conustessulatus PEPTIDE (1)..(31) Xaa at residues 4, 7 and 22 is Glu orgamma-carboxy-Glu; Xaa at residues 16 and 26 is Pro or hydroxy-Pro; Xaaat residue 30 is Trp (D or L) or bromo-Trp (D or L); Xaa at residue 24is Tyr, 125I- Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 223 Ser Cys Ala Xaa Phe Gly Xaa Val Cys Ser Ser Thr AlaCys Cys Xaa 1 5 10 15 Asp Leu Asp Cys Val Xaa Ala Xaa Ser Xaa Ile CysLeu Xaa Xaa 20 25 30 224 273 DNA Conus textile 224 cgattgcagg ggttacgatgcgccgtgtag ctctggcgcg ccatgttgtg attggtggac 60 atgttcagca cgaaccaaccgctgttttta ggctgaccac aagccatccg acatcaccac 120 tctcctcttc agaggcttcaaggctttttg ttctcctttt gaagaatctt tacgagtgaa 180 caaacaagta gaatagcacgtttttccccc tttgaaaaat caataatgga ggttaaacaa 240 aactgtcttc ttcaataaagattttatcat aat 273 225 50 PRT Conus textile 225 Ile Gln Gly Gly Gly AspGlu Arg Gln Lys Ala Lys Ile Asn Phe Leu 1 5 10 15 Ser Arg Ser Asp ArgAsp Cys Arg Gly Tyr Asp Ala Pro Cys Ser Ser 20 25 30 Gly Ala Pro Cys CysAsp Trp Trp Thr Cys Ser Ala Arg Thr Asn Arg 35 40 45 Cys Phe 50 226 29PRT Conus textile PEPTIDE (1)..(29) Xaa at residues 8 and 14 is Pro orhydroxy-Pro; Xaa at residues 18 and 19 is Trp (D or L) or bromo-Trp (Dor L); Xaa at residue 5 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr,O-sulpho-Tyr or O-phospho-Tyr 226 Asp Cys Arg Gly Xaa Asp Ala Xaa CysSer Ser Gly Ala Xaa Cys Cys 1 5 10 15 Asp Xaa Xaa Thr Cys Ser Ala ArgThr Asn Arg Cys Phe 20 25 227 23 PRT Conus textile PEPTIDE (1)..(23) Xaaat residue 16 is Glu or gamma-carboxy-Glu; Xaa at residues 3, 11, 12, 22and 23 is Pro or hydroxy-Pro; Xaa at residues 4, 5 and 14 is Tyr,125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho- Tyr or O-phospho-Tyr 227Asn Cys Xaa Xaa Cys Val Val Xaa Cys Cys Xaa Xaa Ala Xaa Cys Xaa 1 5 1015 Ala Ser Gly Cys Arg Xaa Xaa 20 228 205 DNA Conus textile CDS(1)..(186) 228 atg cac tgt ctc cca atc ttc gtc att ctt ctg ctg ctg actgca tct 48 Met His Cys Leu Pro Ile Phe Val Ile Leu Leu Leu Leu Thr AlaSer 1 5 10 15 gga cct agc gtt gat gcc caa ctg aag acc aaa gat gat gtgccc ctg 96 Gly Pro Ser Val Asp Ala Gln Leu Lys Thr Lys Asp Asp Val ProLeu 20 25 30 tca tct ttc cga gat cat gca aag agt acc cta cga aga ctt caggac 144 Ser Ser Phe Arg Asp His Ala Lys Ser Thr Leu Arg Arg Leu Gln Asp35 40 45 aaa cag act tgc tgt ggc tat agg atg tgt gtt cct tgt ggt 186 LysGln Thr Cys Cys Gly Tyr Arg Met Cys Val Pro Cys Gly 50 55 60 taaccagcatgaaggatcc 205 229 62 PRT Conus textile 229 Met His Cys Leu Pro Ile PheVal Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 Gly Pro Ser Val Asp AlaGln Leu Lys Thr Lys Asp Asp Val Pro Leu 20 25 30 Ser Ser Phe Arg Asp HisAla Lys Ser Thr Leu Arg Arg Leu Gln Asp 35 40 45 Lys Gln Thr Cys Cys GlyTyr Arg Met Cys Val Pro Cys Gly 50 55 60 230 12 PRT Conus textilePEPTIDE (1)..(12) Xaa at residue 1 is Gln or pyro-Gln; Xaa at residue 11is Pro or hydroxy-Pro; Xaa at residue 6 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 230 Xaa Thr Cys Cys Gly XaaArg Met Cys Val Xaa Cys 1 5 10 231 115 DNA Conus textile CDS (2)..(94)231 g tta tgg agc gat tgc tat agt tgg tta gga tca tgt att gcg ccc tcg 49Leu Trp Ser Asp Cys Tyr Ser Trp Leu Gly Ser Cys Ile Ala Pro Ser 1 5 1015 cag tgt tgt tct gag gtt tgt gat tat tac tgc cgc cta tgg cga 94 GlnCys Cys Ser Glu Val Cys Asp Tyr Tyr Cys Arg Leu Trp Arg 20 25 30tgaactcgga ccacaagcca t 115 232 31 PRT Conus textile 232 Leu Trp Ser AspCys Tyr Ser Trp Leu Gly Ser Cys Ile Ala Pro Ser 1 5 10 15 Gln Cys CysSer Glu Val Cys Asp Tyr Tyr Cys Arg Leu Trp Arg 20 25 30 233 28 PRTConus textile PEPTIDE (1)..(28) Xaa at residue 18 is Glu orgamma-carboxy-Glu; Xaa at residue 13 is Pro or hydroxy-Pro; Xaa atresidues 5 and 27 is Trp (D or L) or bromo-Trp (D or L); Xaa at residue3, 22 and 23 is Tyr, 125I-Tyr , mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyror O-phospho-Tyr 233 Asp Cys Xaa Ser Xaa Leu Gly Ser Cys Ile Ala Xaa SerGln Cys Cys 1 5 10 15 Ser Xaa Val Cys Asp Xaa Xaa Cys Arg Leu Xaa Arg 2025 234 279 DNA Conus textile CDS (7)..(126) 234 agctga cga atg aaa aattcc gag aat gtc aag ctc agc aag aga aaa 48 Arg Met Lys Asn Ser Glu AsnVal Lys Leu Ser Lys Arg Lys 1 5 10 tgt gtg gaa caa tgg aaa tac tgc acccga gag tcc tta tgt tgc gcg 96 Cys Val Glu Gln Trp Lys Tyr Cys Thr ArgGlu Ser Leu Cys Cys Ala 15 20 25 30 ggt ttg tgt ttg ttt agt ttc tgc attcta taacgctaat ccagagtcgt 146 Gly Leu Cys Leu Phe Ser Phe Cys Ile Leu 3540 atattccgtc taagctccac ctggcactgt ctggtatgtt cctgccagtg actggtctca 206taccgcttag actctggtcc gtcttctctg caaccacagg agaacgtgca ttattacaat 266aaacgcatac tgc 279 235 40 PRT Conus textile 235 Arg Met Lys Asn Ser GluAsn Val Lys Leu Ser Lys Arg Lys Cys Val 1 5 10 15 Glu Gln Trp Lys TyrCys Thr Arg Glu Ser Leu Cys Cys Ala Gly Leu 20 25 30 Cys Leu Phe Ser PheCys Ile Leu 35 40 236 27 PRT Conus textile PEPTIDE (1)..(27) Xaa atresidues 4 and 12 is Glu or gamma-carboxy-Glu; Xaa at residue 6 is Trp(D or L) or bromo-Trp (D or L); Xaa at residue 8 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 236 Lys CysVal Xaa Gln Xaa Lys Xaa Cys Thr Arg Xaa Ser Leu Cys Cys 1 5 10 15 AlaGly Leu Cys Leu Phe Ser Phe Cys Ile Leu 20 25 237 510 DNA Conus textileCDS (223)..(471) 237 cagagccgct ctggtgtgca gacctgtctc cagccctccgtctccctgat cggtggttct 60 gcctgcatag ctgtcttctc cacgaagctt tccacaggtataaataacgc ttcagtctcc 120 cgtcctgtat tgggccgccg ttacaagcca gaccgatacagccaggtcca gtctactttg 180 cgagtgagtt aaaagctcca gcattctacc agcatcacca gaatg aag gtg agc 234 Met Lys Val Ser 1 agc gtg ctg atc gtg gct acg ctgaca ctg acc gca ggc cag ctg gtt 282 Ser Val Leu Ile Val Ala Thr Leu ThrLeu Thr Ala Gly Gln Leu Val 5 10 15 20 agt gct tct tcc cat tac tca aaagat gtc cag att ctt cct tct gtg 330 Ser Ala Ser Ser His Tyr Ser Lys AspVal Gln Ile Leu Pro Ser Val 25 30 35 aga tca gct gac gaa gtg gaa aat tccgag aat gtc agg ctc agc aag 378 Arg Ser Ala Asp Glu Val Glu Asn Ser GluAsn Val Arg Leu Ser Lys 40 45 50 aga aga tgt gtg gaa caa tgg gaa gtc tgcggc ata atc ttg ttc tcc 426 Arg Arg Cys Val Glu Gln Trp Glu Val Cys GlyIle Ile Leu Phe Ser 55 60 65 tca tca tgt tgc ggg cag ttg tgt ttg ttt ggtttc tgc gtt cta 471 Ser Ser Cys Cys Gly Gln Leu Cys Leu Phe Gly Phe CysVal Leu 70 75 80 taacgctaat ccagagtcgt atattccgtc taagctcca 510 238 83PRT Conus textile 238 Met Lys Val Ser Ser Val Leu Ile Val Ala Thr LeuThr Leu Thr Ala 1 5 10 15 Gly Gln Leu Val Ser Ala Ser Ser His Tyr SerLys Asp Val Gln Ile 20 25 30 Leu Pro Ser Val Arg Ser Ala Asp Glu Val GluAsn Ser Glu Asn Val 35 40 45 Arg Leu Ser Lys Arg Arg Cys Val Glu Gln TrpGlu Val Cys Gly Ile 50 55 60 Ile Leu Phe Ser Ser Ser Cys Cys Gly Gln LeuCys Leu Phe Gly Phe 65 70 75 80 Cys Val Leu 239 29 PRT Conus textilePEPTIDE (1)..(29) Xaa at residues 3 and 6 is Glu or gamma-carboxy-Glu;Xaa at residue 5 is Trp (D or L) or bromo-Trp (D or L) 239 Cys Val XaaGln Xaa Xaa Val Cys Gly Ile Ile Leu Phe Ser Ser Ser 1 5 10 15 Cys CysGly Gln Leu Cys Leu Phe Gly Phe Cys Val Leu 20 25 240 27 PRT Conustextile PEPTIDE (1)..(27) Xaa at residues 9 and 13 is Glu orgamma-carboxy-Glu; Xaa at resi residues 4, 7 and 23 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo- Tyr, O-sulpho-Tyr or O-phospho-Tyr 240 Cys GlyGly Xaa Ser Thr Xaa Cys Xaa Val Asp Ser Xaa Cys Cys Ser 1 5 10 15 AspAsn Cys Val Arg Ser Xaa Cys Thr Leu Phe 20 25 241 27 PRT Conus textilePEPTIDE (1)..(27) Xaa at residues 8 and 13 is Glu or gamma-carboxy-Glu241 Gly Cys Asn Asn Ser Cys Gln Xaa His Ser Asp Cys Xaa Ser His Cys 1 510 15 Ile Cys Thr Ser Arg Gly Cys Gly Ala Val Asn 20 25 242 373 DNAConus tulipa CDS (22)..(258) 242 caggattgaa caaaattcag g atg tca gga ttggga atc atg gtg cta acc 51 Met Ser Gly Leu Gly Ile Met Val Leu Thr 1 510 ctt cta ctt ctt gtg tcc atg gca acc agt cat cgt tat gca aga gaa 99Leu Leu Leu Leu Val Ser Met Ala Thr Ser His Arg Tyr Ala Arg Glu 15 20 25aag cag gcg acg cga agg gac gca gtc aac gtc aga cgg aga agc aga 147 LysGln Ala Thr Arg Arg Asp Ala Val Asn Val Arg Arg Arg Ser Arg 30 35 40 ccaaaa aca aag gag tgc gaa agg tac tgt gag ctg gag gaa aag cac 195 Pro LysThr Lys Glu Cys Glu Arg Tyr Cys Glu Leu Glu Glu Lys His 45 50 55 tgc tgctgc ata aga agt aac gga ccc aaa tgt tcc aga ata tgc ata 243 Cys Cys CysIle Arg Ser Asn Gly Pro Lys Cys Ser Arg Ile Cys Ile 60 65 70 ttc aaa ttttgg tgt tagttttctg tacactgtcc attcattatc ttatcagtac 298 Phe Lys Phe TrpCys 75 aagtgtaaac gagacatgtc agaaagtcga aggttgtgcg taatttgata agcattgttt358 actgggacga acgga 373 243 79 PRT Conus tulipa 243 Met Ser Gly Leu GlyIle Met Val Leu Thr Leu Leu Leu Leu Val Ser 1 5 10 15 Met Ala Thr SerHis Arg Tyr Ala Arg Glu Lys Gln Ala Thr Arg Arg 20 25 30 Asp Ala Val AsnVal Arg Arg Arg Ser Arg Pro Lys Thr Lys Glu Cys 35 40 45 Glu Arg Tyr CysGlu Leu Glu Glu Lys His Cys Cys Cys Ile Arg Ser 50 55 60 Asn Gly Pro LysCys Ser Arg Ile Cys Ile Phe Lys Phe Trp Cys 65 70 75 244 37 PRT Conustulipa PEPTIDE (1)..(37) Xaa at residues 5, 7, 11, 13 and 14 is Glu orgamma-carboxy-Glu; Xaa at residues 1 and 25 is Pro or hydroxy-Pro; Xaaat residue 36 is Trp (D or L) or bromo-Trp (D or L); 244 Xaa Lys Thr LysXaa Cys Xaa Arg Xaa Cys Xaa Leu Xaa Xaa Lys His 1 5 10 15 Cys Cys CysIle Arg Ser Asn Gly Xaa Lys Cys Ser Arg Ile Cys Ile 20 25 30 Phe Lys PheXaa Cys 35 245 381 DNA Conus tulipa CDS (22)..(267) 245 caggattgaacaaaattcag g atg tca gga ttg gga atc atg gtg cta acc 51 Met Ser Gly LeuGly Ile Met Val Leu Thr 1 5 10 ctt ctc ctt ctt gtg cta atg aca acc agtcat cag gat gca gga gag 99 Leu Leu Leu Leu Val Leu Met Thr Thr Ser HisGln Asp Ala Gly Glu 15 20 25 aag cag gcg atg caa agg gac gca aag aac ttcagt cgg aga aga tta 147 Lys Gln Ala Met Gln Arg Asp Ala Lys Asn Phe SerArg Arg Arg Leu 30 35 40 gtc att cgg aga cca aaa aca agg gag tgc gaa atgcag tgt gag cag 195 Val Ile Arg Arg Pro Lys Thr Arg Glu Cys Glu Met GlnCys Glu Gln 45 50 55 gag gag aaa cac tgc tgc cgc gta aga gat ggt acg ggccaa tgt gcc 243 Glu Glu Lys His Cys Cys Arg Val Arg Asp Gly Thr Gly GlnCys Ala 60 65 70 cct aag tgc ttg gga att aac tgg tagtttctgt acactgtctcattcattatc 297 Pro Lys Cys Leu Gly Ile Asn Trp 75 80 ttatcagtacacgtgtaacg agacatgtca gaaagtcgaa ggtagtgcgt aatttgataa 357 gcattgtttactgggacgaa cgga 381 246 82 PRT Conus tulipa 246 Met Ser Gly Leu Gly IleMet Val Leu Thr Leu Leu Leu Leu Val Leu 1 5 10 15 Met Thr Thr Ser HisGln Asp Ala Gly Glu Lys Gln Ala Met Gln Arg 20 25 30 Asp Ala Lys Asn PheSer Arg Arg Arg Leu Val Ile Arg Arg Pro Lys 35 40 45 Thr Arg Glu Cys GluMet Gln Cys Glu Gln Glu Glu Lys His Cys Cys 50 55 60 Arg Val Arg Asp GlyThr Gly Gln Cys Ala Pro Lys Cys Leu Gly Ile 65 70 75 80 Asn Trp 247 36PRT Conus tulipa PEPTIDE (1)..(36) Xaa at residues 5, 7, 11, 13 and 14is Glu or gamma-carboxy-Glu; Xaa at residues 1 and 29 is Pro orhydroxy-Pro; Xaa at residue 36 is Trp (D or L) or bromo-Trp (D or L) 247Xaa Lys Thr Arg Xaa Cys Xaa Met Gln Cys Xaa Gln Xaa Xaa Lys His 1 5 1015 Cys Cys Arg Val Arg Asp Gly Thr Gly Gln Cys Ala Xaa Lys Cys Leu 20 2530 Gly Ile Asn Xaa 35 248 363 DNA Conus tulipa CDS (1)..(264) 248 atgatg tcg aaa atg gga gct atg ttt gtc ctt ttg ctt ctt ttc acc 48 Met MetSer Lys Met Gly Ala Met Phe Val Leu Leu Leu Leu Phe Thr 1 5 10 15 ctggca tcc agc cag cag gaa gga gat gtc cag gca agg aaa aca cgc 96 Leu AlaSer Ser Gln Gln Glu Gly Asp Val Gln Ala Arg Lys Thr Arg 20 25 30 ctg aagagc gac ttc tat cgt gct ctg cca agg ttt ggc cca ata tgc 144 Leu Lys SerAsp Phe Tyr Arg Ala Leu Pro Arg Phe Gly Pro Ile Cys 35 40 45 act tgt tttaaa agc cag aac tgt cgg ggt tct tgt gaa tgc atg tca 192 Thr Cys Phe LysSer Gln Asn Cys Arg Gly Ser Cys Glu Cys Met Ser 50 55 60 cct ccc ggt tgttac tgc agt aac aat ggc att cgt gaa cga gga tgc 240 Pro Pro Gly Cys TyrCys Ser Asn Asn Gly Ile Arg Glu Arg Gly Cys 65 70 75 80 tcg tgt aca tgtcca ggg act ggt tgaatgattt gaaaaattca gagcaatatg 294 Ser Cys Thr Cys ProGly Thr Gly 85 ttgcagaaaa accgaagacc gagacttctc acaataaatc cataaagacattaaaaaaaa 354 aaaaaaaaa 363 249 88 PRT Conus tulipa 249 Met Met Ser LysMet Gly Ala Met Phe Val Leu Leu Leu Leu Phe Thr 1 5 10 15 Leu Ala SerSer Gln Gln Glu Gly Asp Val Gln Ala Arg Lys Thr Arg 20 25 30 Leu Lys SerAsp Phe Tyr Arg Ala Leu Pro Arg Phe Gly Pro Ile Cys 35 40 45 Thr Cys PheLys Ser Gln Asn Cys Arg Gly Ser Cys Glu Cys Met Ser 50 55 60 Pro Pro GlyCys Tyr Cys Ser Asn Asn Gly Ile Arg Glu Arg Gly Cys 65 70 75 80 Ser CysThr Cys Pro Gly Thr Gly 85 250 44 PRT Conus tulipa PEPTIDE (1)..(44) Xaaat residues 18 and 34 is Glu or gamma-carboxy-Glu; Xaa at residues 3,22, 23 and 42 is Pro or hydroxy-Pro; Xaa at residue 26 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 250 Phe GlyXaa Ile Cys Thr Cys Phe Lys Ser Gln Asn Cys Arg Gly Ser 1 5 10 15 CysXaa Cys Met Ser Xaa Xaa Gly Cys Xaa Cys Ser Asn Asn Gly Ile 20 25 30 ArgXaa Arg Gly Cys Ser Cys Thr Cys Xaa Gly Thr 35 40 251 383 DNA Conustulipa CDS (1)..(276) 251 atg atg tcg aaa atg gga gct atg ttt gtc cttttg ctt ctt ttc acc 48 Met Met Ser Lys Met Gly Ala Met Phe Val Leu LeuLeu Leu Phe Thr 1 5 10 15 ctg gca tcc agc cag cag gaa gga gat gtc caggca agg aaa aca cgc 96 Leu Ala Ser Ser Gln Gln Glu Gly Asp Val Gln AlaArg Lys Thr Arg 20 25 30 ctg aag agc gac ttc tat cgt act ctg gca ata tctgac aga gga tgc 144 Leu Lys Ser Asp Phe Tyr Arg Thr Leu Ala Ile Ser AspArg Gly Cys 35 40 45 act ggc aac tgt gat tgg acg tgt agc ggt gat tgc agctgc cag ggc 192 Thr Gly Asn Cys Asp Trp Thr Cys Ser Gly Asp Cys Ser CysGln Gly 50 55 60 aca tct gac tcg tgt cac tgc att cca cca aaa tca ata ggcaac aga 240 Thr Ser Asp Ser Cys His Cys Ile Pro Pro Lys Ser Ile Gly AsnArg 65 70 75 80 tgc cgg tgt cag tgt aaa aga aaa atc gaa att gactgattctttt 286 Cys Arg Cys Gln Cys Lys Arg Lys Ile Glu Ile Asp 85 90aactcgttga acgatttaaa aatcagacca atatgtaggc agaaaaccga agactctgag 346actctcgtaa taatcgtaag caaaaaaaaa aaaaaaa 383 252 92 PRT Conus tulipa 252Met Met Ser Lys Met Gly Ala Met Phe Val Leu Leu Leu Leu Phe Thr 1 5 1015 Leu Ala Ser Ser Gln Gln Glu Gly Asp Val Gln Ala Arg Lys Thr Arg 20 2530 Leu Lys Ser Asp Phe Tyr Arg Thr Leu Ala Ile Ser Asp Arg Gly Cys 35 4045 Thr Gly Asn Cys Asp Trp Thr Cys Ser Gly Asp Cys Ser Cys Gln Gly 50 5560 Thr Ser Asp Ser Cys His Cys Ile Pro Pro Lys Ser Ile Gly Asn Arg 65 7075 80 Cys Arg Cys Gln Cys Lys Arg Lys Ile Glu Ile Asp 85 90 253 46 PRTConus tulipa PEPTIDE (1)..(46) Xaa at residue 44 is Glu orgamma-carboxy-Glu; Xaa at residues 27 and 28 is Pro or hydroxy-Pro; Xaaat residue 8 is Trp (D or L) or bromo-Trp (D or L) 253 Gly Cys Thr GlyAsn Cys Asp Xaa Thr Cys Ser Gly Asp Cys Ser Cys 1 5 10 15 Gln Gly ThrSer Asp Ser Cys His Cys Ile Xaa Xaa Lys Ser Ile Gly 20 25 30 Asn Arg CysArg Cys Gln Cys Lys Arg Lys Ile Xaa Ile Asp 35 40 45 254 404 DNA Conusvirgo CDS (7)..(243) 254 ggatcc atg aaa ctg acg tgt gtg gtg atc atc actgtg ctg ttc ctg 48 Met Lys Leu Thr Cys Val Val Ile Ile Thr Val Leu PheLeu 1 5 10 acg gcc agt cag ctc att aca gct gat tac tcc aga gat cag cggcag 96 Thr Ala Ser Gln Leu Ile Thr Ala Asp Tyr Ser Arg Asp Gln Arg Gln15 20 25 30 tac cgt gca gtg agg ttg gga gat gaa atg cgg aat ttc aaa ggtgcc 144 Tyr Arg Ala Val Arg Leu Gly Asp Glu Met Arg Asn Phe Lys Gly Ala35 40 45 agg gac tgc ggg gga caa ggt gaa ggt tgt tat act caa cct tgc tgc192 Arg Asp Cys Gly Gly Gln Gly Glu Gly Cys Tyr Thr Gln Pro Cys Cys 5055 60 cct ggt ctg cgg tgc cgt ggc ggc ggt act gga gga ggc gta tgc cag240 Pro Gly Leu Arg Cys Arg Gly Gly Gly Thr Gly Gly Gly Val Cys Gln 6570 75 ctg tagtaatagt ttggcatctg atatttcccc tctgtgctcc accctctttt 293 Leugcctgattca tccttaccta tgtgtggtca tgaaccactc agtagctaca cctctggtgg 353attcagagaa cgtatatcaa aataaaacca cattgcaata aaaaaaaaaa a 404 255 79 PRTConus virgo 255 Met Lys Leu Thr Cys Val Val Ile Ile Thr Val Leu Phe LeuThr Ala 1 5 10 15 Ser Gln Leu Ile Thr Ala Asp Tyr Ser Arg Asp Gln ArgGln Tyr Arg 20 25 30 Ala Val Arg Leu Gly Asp Glu Met Arg Asn Phe Lys GlyAla Arg Asp 35 40 45 Cys Gly Gly Gln Gly Glu Gly Cys Tyr Thr Gln Pro CysCys Pro Gly 50 55 60 Leu Arg Cys Arg Gly Gly Gly Thr Gly Gly Gly Val CysGln Leu 65 70 75 256 32 PRT Conus virgo PEPTIDE (1)..(32) Xaa at residue7 is Glu or gamma-carboxy-Glu; Xaa at residues 13 and 16 is Pro orhydroxy-Pro; Xaa at residue 10 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 256 Asp Cys Gly Gly Gln GlyXaa Gly Cys Xaa Thr Gln Xaa Cys Cys Xaa 1 5 10 15 Gly Leu Arg Cys ArgGly Gly Gly Thr Gly Gly Gly Val Cys Gln Leu 20 25 30 257 285 DNA Conusradiatus CDS (4)..(225) 257 atc atg cag aaa ctg aca atc ctg ctt ctt gttgct gct ata ctg atg 48 Met Gln Lys Leu Thr Ile Leu Leu Leu Val Ala AlaIle Leu Met 1 5 10 15 tcg acc cag gtc ctg att caa ggt ggt gga gaa aaacgc caa aaa gtc 96 Ser Thr Gln Val Leu Ile Gln Gly Gly Gly Glu Lys ArgGln Lys Val 20 25 30 aac att ttt tca aaa aga aag aca gat gct gag acc tggtgg gag ggc 144 Asn Ile Phe Ser Lys Arg Lys Thr Asp Ala Glu Thr Trp TrpGlu Gly 35 40 45 gaa tgc tct aat tgg tta gga agt tgt tcg acg ccc tca aattgc tgt 192 Glu Cys Ser Asn Trp Leu Gly Ser Cys Ser Thr Pro Ser Asn CysCys 50 55 60 ctc aag agt tgt aat ggg cac tgc aca ttg tgg tgatgaactctgaccacaaa 245 Leu Lys Ser Cys Asn Gly His Cys Thr Leu Trp 65 70gccatccaac atcaccgctc tcctcttcag agtcttcaag 285 258 74 PRT Conusradiatus 258 Met Gln Lys Leu Thr Ile Leu Leu Leu Val Ala Ala Ile Leu MetSer 1 5 10 15 Thr Gln Val Leu Ile Gln Gly Gly Gly Glu Lys Arg Gln LysVal Asn 20 25 30 Ile Phe Ser Lys Arg Lys Thr Asp Ala Glu Thr Trp Trp GluGly Glu 35 40 45 Cys Ser Asn Trp Leu Gly Ser Cys Ser Thr Pro Ser Asn CysCys Leu 50 55 60 Lys Ser Cys Asn Gly His Cys Thr Leu Trp 65 70 259 31PRT Conus radiatus PEPTIDE (1)..(31) Xaa at residues 3 and is Glu orgamma-carboxy-Glu; Xaa at residue 16 is Pro or hydroxy-Pro; Xaa atresidues 1, 2, 9 and 31 is Trp (D or L) or bromo-Trp (D or L) 259 XaaXaa Xaa Gly Xaa Cys Ser Asn Xaa Leu Gly Ser Cys Ser Thr Xaa 1 5 10 15Ser Asn Cys Cys Leu Lys Ser Cys Asn Gly His Cys Thr Leu Xaa 20 25 30 260296 DNA Conus radiatus CDS (1)..(237) 260 atc atg cag aaa ctg ata atcctg ctt ctt gtt gct gct gta ctg atg 48 Ile Met Gln Lys Leu Ile Ile LeuLeu Leu Val Ala Ala Val Leu Met 1 5 10 15 tcc acc cag gcc ctg att caaggt ggt gga gga aaa cgc caa cag gca 96 Ser Thr Gln Ala Leu Ile Gln GlyGly Gly Gly Lys Arg Gln Gln Ala 20 25 30 aag agc aag tat ttt tcc gaa agaaag gca cct gct aag cgt tgg ttt 144 Lys Ser Lys Tyr Phe Ser Glu Arg LysAla Pro Ala Lys Arg Trp Phe 35 40 45 gga cac gaa gaa tgc act tat tgg ttgggg cct tgt gag gtg gac gac 192 Gly His Glu Glu Cys Thr Tyr Trp Leu GlyPro Cys Glu Val Asp Asp 50 55 60 acg tgt tgt tct gcc agt tgt gag tcc aagttc tgc ggg ttg tgg 237 Thr Cys Cys Ser Ala Ser Cys Glu Ser Lys Phe CysGly Leu Trp 65 70 75 tgatggacac tgaccacaag tcatcctaca tcgccactctcctgttcaga gtcttcaag 296 261 79 PRT Conus radiatus 261 Ile Met Gln LysLeu Ile Ile Leu Leu Leu Val Ala Ala Val Leu Met 1 5 10 15 Ser Thr GlnAla Leu Ile Gln Gly Gly Gly Gly Lys Arg Gln Gln Ala 20 25 30 Lys Ser LysTyr Phe Ser Glu Arg Lys Ala Pro Ala Lys Arg Trp Phe 35 40 45 Gly His GluGlu Cys Thr Tyr Trp Leu Gly Pro Cys Glu Val Asp Asp 50 55 60 Thr Cys CysSer Ala Ser Cys Glu Ser Lys Phe Cys Gly Leu Trp 65 70 75 262 33 PRTConus radiatus PEPTIDE (1)..(33) Xaa at residues 5, 6, 15 adn 26 is Gluor gamma-carboxy-Glu; Xaa at residue 13 is Pro or hydroxy-Pro; Xaa atresidues 1, 10 and 33 is Trp (D or L) or bromo-Trp (D or L); 262 Xaa PheGly His Xaa Xaa Cys Thr Xaa Xaa Leu Gly Xaa Cys Xaa Val 1 5 10 15 AspAsp Thr Cys Cys Ser Ala Ser Cys Xaa Ser Lys Phe Cys Gly Leu 20 25 30 Xaa263 306 DNA Conus wittigi CDS (7)..(231) 263 ggatcc atg aaa ctg acg tgtgtg gtg atc atc gcc ttg ctg ttc ctg 48 Met Lys Leu Thr Cys Val Val IleIle Ala Leu Leu Phe Leu 1 5 10 acg gcc tgt cag ctc att acg gct gat tactcc aga gat gag cag tct 96 Thr Ala Cys Gln Leu Ile Thr Ala Asp Tyr SerArg Asp Glu Gln Ser 15 20 25 30 ggc agt aca gtg cgg ttt cta gac aga ccacgg cgt ttt ggt tcg ttc 144 Gly Ser Thr Val Arg Phe Leu Asp Arg Pro ArgArg Phe Gly Ser Phe 35 40 45 ata ccg tgc gcc cgt tta ggt gaa cca tgt accata tgc tgc cgt cct 192 Ile Pro Cys Ala Arg Leu Gly Glu Pro Cys Thr IleCys Cys Arg Pro 50 55 60 ttg agg tgc cgt gaa agc gga aca ccc aca tgt caagtg tgattgtctg 241 Leu Arg Cys Arg Glu Ser Gly Thr Pro Thr Cys Gln Val65 70 75 gcatctgata tttcccctct gtgccctacc ctcttttgcc tgagtcatccatacctgtgc 301 tcgag 306 264 75 PRT Conus wittigi 264 Met Lys Leu ThrCys Val Val Ile Ile Ala Leu Leu Phe Leu Thr Ala 1 5 10 15 Cys Gln LeuIle Thr Ala Asp Tyr Ser Arg Asp Glu Gln Ser Gly Ser 20 25 30 Thr Val ArgPhe Leu Asp Arg Pro Arg Arg Phe Gly Ser Phe Ile Pro 35 40 45 Cys Ala ArgLeu Gly Glu Pro Cys Thr Ile Cys Cys Arg Pro Leu Arg 50 55 60 Cys Arg GluSer Gly Thr Pro Thr Cys Gln Val 65 70 75 265 33 PRT Conus wittigiPEPTIDE (1)..(33) Xaa at residues 12 and 25 is Glu or gamma-carboxy-Glu;Xaa at residues 6, 13, 20 and 29 is Pro or hydroxy-Pro 265 Phe Gly SerPhe Ile Xaa Cys Ala Arg Leu Gly Xaa Xaa Cys Thr Ile 1 5 10 15 Cys CysArg Xaa Leu Arg Cys Arg Xaa Ser Gly Thr Xaa Thr Cys Gln 20 25 30 Val 266381 DNA Conus regius CDS (7)..(240) 266 ggatcc atg aaa ctg acg tgc gtggtg atc atg gcc tcg ctg ttc ctg 48 Met Lys Leu Thr Cys Val Val Ile MetAla Ser Leu Phe Leu 1 5 10 gcg gcc tgt caa ttc ctt aca gct gga ggt gactca aga agt aag cag 96 Ala Ala Cys Gln Phe Leu Thr Ala Gly Gly Asp SerArg Ser Lys Gln 15 20 25 30 cgg tat cct gat tgg agg ctg ggc tac cga aagtcc aag ttg atg gct 144 Arg Tyr Pro Asp Trp Arg Leu Gly Tyr Arg Lys SerLys Leu Met Ala 35 40 45 aag aag acg tgc ctg gaa cat aac aaa cta tgt tggtat gat aga gac 192 Lys Lys Thr Cys Leu Glu His Asn Lys Leu Cys Trp TyrAsp Arg Asp 50 55 60 tgc tgc acc ata tat tgt aat gaa aac aaa tgc ggc gtgaaa cct caa 240 Cys Cys Thr Ile Tyr Cys Asn Glu Asn Lys Cys Gly Val LysPro Gln 65 70 75 tgaatgtttc acacacacac acacacacac acacacacac acacacacacacacacacac 300 acacacacac atctggcgtc tgaccattcc ccctctgtgc tctatcctcttgttcctgag 360 tcatccatac ctgtgctcga g 381 267 78 PRT Conus regius 267Met Lys Leu Thr Cys Val Val Ile Met Ala Ser Leu Phe Leu Ala Ala 1 5 1015 Cys Gln Phe Leu Thr Ala Gly Gly Asp Ser Arg Ser Lys Gln Arg Tyr 20 2530 Pro Asp Trp Arg Leu Gly Tyr Arg Lys Ser Lys Leu Met Ala Lys Lys 35 4045 Thr Cys Leu Glu His Asn Lys Leu Cys Trp Tyr Asp Arg Asp Cys Cys 50 5560 Thr Ile Tyr Cys Asn Glu Asn Lys Cys Gly Val Lys Pro Gln 65 70 75 26830 PRT Conus regius PEPTIDE (1)..(30) Xaa at residues 4 and 22 is Glu orgamma-carboxy-Glu; Xaa at residue 29 is Pro or hydroxy-Pro; Xaa atresidue 10 is Trp (D or L) or bromo-Trp (D or L); Xaa at residues 11 and19 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 268 Thr Cys Leu Xaa His Asn Lys Leu Cys Xaa Xaa Asp ArgAsp Cys Cys 1 5 10 15 Thr Ile Xaa Cys Asn Xaa Asn Lys Cys Gly Val LysXaa Gln 20 25 30 269 285 DNA Conus radiatus CDS (1)..(225) 269 atc atgcag aaa ctg aca atc ctg ctt ctt gtt gct gct ata ctg atg 48 Ile Met GlnLys Leu Thr Ile Leu Leu Leu Val Ala Ala Ile Leu Met 1 5 10 15 tcg acccag gtc ctg att caa ggt ggt gga gaa aaa cgc caa aaa gtc 96 Ser Thr GlnVal Leu Ile Gln Gly Gly Gly Glu Lys Arg Gln Lys Val 20 25 30 aac att ttttca aaa aga aag aca gat gct gag acc tgg tgg gag ggc 144 Asn Ile Phe SerLys Arg Lys Thr Asp Ala Glu Thr Trp Trp Glu Gly 35 40 45 gaa tgc tct aattgg tta gga agt tgt tcg acg ccc tca aat tgc tgt 192 Glu Cys Ser Asn TrpLeu Gly Ser Cys Ser Thr Pro Ser Asn Cys Cys 50 55 60 ctc aag agt tgt aatggg cac tgc aca ttg tgg tgatgaactc tgaccacaaa 245 Leu Lys Ser Cys AsnGly His Cys Thr Leu Trp 65 70 75 gccatccaac atcaccgctc tcctcttcagagtcttcaag 285 270 75 PRT Conus radiatus 270 Ile Met Gln Lys Leu Thr IleLeu Leu Leu Val Ala Ala Ile Leu Met 1 5 10 15 Ser Thr Gln Val Leu IleGln Gly Gly Gly Glu Lys Arg Gln Lys Val 20 25 30 Asn Ile Phe Ser Lys ArgLys Thr Asp Ala Glu Thr Trp Trp Glu Gly 35 40 45 Glu Cys Ser Asn Trp LeuGly Ser Cys Ser Thr Pro Ser Asn Cys Cys 50 55 60 Leu Lys Ser Cys Asn GlyHis Cys Thr Leu Trp 65 70 75 271 31 PRT Conus radiatus PEPTIDE (1)..(31)Xaa at residues 3 and 5 is Glu or gamma-carboxy-Glu; Xaa at residue 16is Pro or hydroxy-Pro; Xaa at residues 1,2, 9 and 31 is Trp (D or L) orbromo-Trp (D or L) 271 Xaa Xaa Xaa Gly Xaa Cys Ser Asn Xaa Leu Gly SerCys Ser Thr Xaa 1 5 10 15 Ser Asn Cys Cys Leu Lys Ser Cys Asn Gly HisCys Thr Leu Xaa 20 25 30 272 296 DNA Conus radiatus CDS (4)..(237) 272atc atg cag aaa ctg ata atc ctg ctt ctt gtt gct gct gta ctg atg 48 MetGln Lys Leu Ile Ile Leu Leu Leu Val Ala Ala Val Leu Met 1 5 10 15 tccacc cag gcc ctg att caa ggt ggt gga gga aaa cgc caa cag gca 96 Ser ThrGln Ala Leu Ile Gln Gly Gly Gly Gly Lys Arg Gln Gln Ala 20 25 30 aag agcaag tat ttt tcc gaa aga aag gca cct gct aag cgt tgg ttt 144 Lys Ser LysTyr Phe Ser Glu Arg Lys Ala Pro Ala Lys Arg Trp Phe 35 40 45 gga cac gaagaa tgc act tat tgg ttg ggg cct tgt gag gtg gac gac 192 Gly His Glu GluCys Thr Tyr Trp Leu Gly Pro Cys Glu Val Asp Asp 50 55 60 acg tgt tgt tctgcc agt tgt gag tcc aag ttc tgc ggg ttg tgg 237 Thr Cys Cys Ser Ala SerCys Glu Ser Lys Phe Cys Gly Leu Trp 65 70 75 tgatggacac tgaccacaagtcatcctaca tcgccactct cctgttcaga gtcttcaag 296 273 78 PRT Conus radiatus273 Met Gln Lys Leu Ile Ile Leu Leu Leu Val Ala Ala Val Leu Met Ser 1 510 15 Thr Gln Ala Leu Ile Gln Gly Gly Gly Gly Lys Arg Gln Gln Ala Lys 2025 30 Ser Lys Tyr Phe Ser Glu Arg Lys Ala Pro Ala Lys Arg Trp Phe Gly 3540 45 His Glu Glu Cys Thr Tyr Trp Leu Gly Pro Cys Glu Val Asp Asp Thr 5055 60 Cys Cys Ser Ala Ser Cys Glu Ser Lys Phe Cys Gly Leu Trp 65 70 75274 33 PRT Conus radiatus PEPTIDE (1)..(33) Xaa at residues 5, 6, 15 and26 is Glu or gamma-carboxy-Glu; Xaa at residue 13 is Pro or hydroxy-Pro;Xaa at residues 1, 10 and 33 is Trp (D or L) or bromo-Trp (D or L); 274Xaa Phe Gly His Xaa Xaa Cys Thr Xaa Xaa Leu Gly Xaa Cys Xaa Val 1 5 1015 Asp Asp Thr Cys Cys Ser Ala Ser Cys Xaa Ser Lys Phe Cys Gly Leu 20 2530 Xaa 275 387 DNA Conus spurius CDS (21)..(212) 275 ggaagctgactacaagcaga atg cgc tgt ctc cca gtc ttc gtc att ctt ctg 53 Met Arg CysLeu Pro Val Phe Val Ile Leu Leu 1 5 10 ctg ctg att cca tct gca cct agcact gat gcc cga ccg aag acc aaa 101 Leu Leu Ile Pro Ser Ala Pro Ser ThrAsp Ala Arg Pro Lys Thr Lys 15 20 25 gat gat gtg cgc ctg gca tct ttc cacggt aag gca aag cga acc cta 149 Asp Asp Val Arg Leu Ala Ser Phe His GlyLys Ala Lys Arg Thr Leu 30 35 40 caa ata cct agg ggg aat atc cac tgt tgcaca aaa tat cag ccg tgc 197 Gln Ile Pro Arg Gly Asn Ile His Cys Cys ThrLys Tyr Gln Pro Cys 45 50 55 tgt tct tca cca tca taaagggaaa tgactttgatgagacccctg cgaactgtcc 252 Cys Ser Ser Pro Ser 60 ctggatgtga aatttggaaacgagactgtt cctttcgcgc gtgttcgtgg aatttcgaat 312 ggtcgttaat aacacgctgcctcttgcaaa ctacaatctc tctgtccttt atctgtggac 372 tggatgtcaa cactg 387 27664 PRT Conus spurius 276 Met Arg Cys Leu Pro Val Phe Val Ile Leu Leu LeuLeu Ile Pro Ser 1 5 10 15 Ala Pro Ser Thr Asp Ala Arg Pro Lys Thr LysAsp Asp Val Arg Leu 20 25 30 Ala Ser Phe His Gly Lys Ala Lys Arg Thr LeuGln Ile Pro Arg Gly 35 40 45 Asn Ile His Cys Cys Thr Lys Tyr Gln Pro CysCys Ser Ser Pro Ser 50 55 60 277 17 PRT Conus spurius PEPTIDE (1)..(17)Xaa at residues 11 and 16 is Pro or hydroxy-Pro; Xaa at residue 9 isTyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr277 Gly Asn Ile His Cys Cys Thr Lys Xaa Gln Xaa Cys Cys Ser Ser Xaa 1 510 15 Ser 278 206 DNA Conus nobilis CDS (1)..(183) 278 atg cgc tgt ctccca gtc ttc gtc att ctt ctg ctg ctg act gca tct 48 Met Arg Cys Leu ProVal Phe Val Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 gca cca agc gttgat gcc cga ccg aag acc aaa gat gat gtg ctc cgg 96 Ala Pro Ser Val AspAla Arg Pro Lys Thr Lys Asp Asp Val Leu Arg 20 25 30 gca tct ttc cgc gataat gca aag agt acc cta caa aga ctt tgg aac 144 Ala Ser Phe Arg Asp AsnAla Lys Ser Thr Leu Gln Arg Leu Trp Asn 35 40 45 aaa cgc atc tgc tgc cccata att ctt tgg tgc tgt ggt taaccagcat 193 Lys Arg Ile Cys Cys Pro IleIle Leu Trp Cys Cys Gly 50 55 60 gaagttccca gga 206 279 61 PRT Conusnobilis 279 Met Arg Cys Leu Pro Val Phe Val Ile Leu Leu Leu Leu Thr AlaSer 1 5 10 15 Ala Pro Ser Val Asp Ala Arg Pro Lys Thr Lys Asp Asp ValLeu Arg 20 25 30 Ala Ser Phe Arg Asp Asn Ala Lys Ser Thr Leu Gln Arg LeuTrp Asn 35 40 45 Lys Arg Ile Cys Cys Pro Ile Ile Leu Trp Cys Cys Gly 5055 60 280 10 PRT Conus nobilis PEPTIDE (1)..(10) Xaa at residue 4 is Proor hydroxy-Pro; Xaa at residue 8 is Trp (D or L) or bromo-Trp (D or L)280 Ile Cys Cys Xaa Ile Ile Leu Xaa Cys Cys 1 5 10 281 205 DNA Conusbetulinus CDS (1)..(183) misc_feature (1)..(205) n is unknown 281 atgcgc tgt ctc cca gtc ttc atc att ctt ctg gtg ctg att gca tct 48 Met ArgCys Leu Pro Val Phe Ile Ile Leu Leu Val Leu Ile Ala Ser 1 5 10 15 gcacct acc gtt gat gcc cga cca aag atc gaa gat gat gag tcc ctg 96 Ala ProThr Val Asp Ala Arg Pro Lys Ile Glu Asp Asp Glu Ser Leu 20 25 30 gca tctttc cat gnt cat naa cca cca tna nng ntn can ctt ttg aac 144 Ala Ser PheHis Xaa His Xaa Pro Pro Xaa Xaa Xaa Thr Leu Leu Asn 35 40 45 aaa cgc aattgc tgc cca gac tct cct ccg tgc tgt cat taaccagcat 193 Lys Arg Asn CysCys Pro Asp Ser Pro Pro Cys Cys His 50 55 60 gaaggttcag ga 205 282 61PRT Conus betulinus misc_feature (1)..(61) Xaa is unknown 282 Met ArgCys Leu Pro Val Phe Ile Ile Leu Leu Val Leu Ile Ala Ser 1 5 10 15 AlaPro Thr Val Asp Ala Arg Pro Lys Ile Glu Asp Asp Glu Ser Leu 20 25 30 AlaSer Phe His Xaa His Xaa Pro Pro Xaa Xaa Xaa Thr Leu Leu Asn 35 40 45 LysArg Asn Cys Cys Pro Asp Ser Pro Pro Cys Cys His 50 55 60 283 11 PRTConus betulinus PEPTIDE (1)..(11) Xaa at residues 4, 7 and 8 is Pro orhydroxy-Pro 283 Asn Cys Cys Xaa Asp Ser Xaa Xaa Cys Cys His 1 5 10 284569 DNA Conus purpurascens 284 ggaattccaa atgatgtaat tactgactacatggtcatag tgtataccca ttgaaaaatt 60 tctatgacat ttcagttgtt agatcatccagttccacaga tggaaagaca gagagatagt 120 agcttgcaag tggcagcgtg ttgttaacgaccattcgaca ttccatgaac acgtgtgaaa 180 ggagcagtct gctttccaaa tctgacatccagggacagtt tgcaggggtc tcatccaaag 240 tcatcttcct ttatcccaaa gtacagcaccgcatctgttt tggacagcaa ccgcgtttct 300 tccaaaatct ttgtagggtt ccttttgcattatcgtggaa agatgccagg ggcatatcat 360 ctttggtctt cggatgagca tcaacgcaaggtgcagatgg aatcagcagc agaagaatga 420 cgaagactgg cagacagcgc attctgcttgtagtcagctt ccgaattcca agccgaattc 480 tgcagatatc catcacactg gcggccgctcgagcatgcat ctagagggcc caattcgccc 540 tatagtgagt cgtatgacaa ttcactggc 569285 63 PRT Conus purpurascens 285 Met Arg Cys Leu Pro Val Phe Val IleLeu Leu Leu Leu Ile Pro Ser 1 5 10 15 Ala Pro Cys Val Asp Ala His ProLys Thr Lys Asp Asp Met Pro Leu 20 25 30 Ala Ser Phe His Asp Asn Ala LysGly Thr Leu Gln Arg Phe Trp Lys 35 40 45 Lys Arg Gly Cys Cys Pro Lys GlnMet Arg Cys Cys Thr Leu Gly 50 55 60 286 12 PRT Conus purpurascensPEPTIDE (1)..(12) Xaa at residue 4 is Pro or hydroxy-Pro 286 Gly Cys CysXaa Lys Gln Met Arg Cys Cys Thr Leu 1 5 10 287 221 DNA Conus ammiralisCDS (21)..(206) 287 ggaagctgac tacaagcaga atg cac tgt ctc cca gtc gtcgtc att ctt ctg 53 Met His Cys Leu Pro Val Val Val Ile Leu Leu 1 5 10ctg ctg act gca tct ggt gga cct agc gtt gat gcc cga ctg aag acc 101 LeuLeu Thr Ala Ser Gly Gly Pro Ser Val Asp Ala Arg Leu Lys Thr 15 20 25 aaagat gat gtg ccc ctg tca tct ttc cgc gat aat aca aag agt atc 149 Lys AspAsp Val Pro Leu Ser Ser Phe Arg Asp Asn Thr Lys Ser Ile 30 35 40 cta caaaga ctt tgg aag cga ggc aac tgc tgt gaa ttt tgg gag ttt 197 Leu Gln ArgLeu Trp Lys Arg Gly Asn Cys Cys Glu Phe Trp Glu Phe 45 50 55 tgc tgt gattaaccagcat gaagg 221 Cys Cys Asp 60 288 62 PRT Conus ammiralis 288 MetHis Cys Leu Pro Val Val Val Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15Gly Gly Pro Ser Val Asp Ala Arg Leu Lys Thr Lys Asp Asp Val Pro 20 25 30Leu Ser Ser Phe Arg Asp Asn Thr Lys Ser Ile Leu Gln Arg Leu Trp 35 40 45Lys Arg Gly Asn Cys Cys Glu Phe Trp Glu Phe Cys Cys Asp 50 55 60 289 12PRT Conus ammiralis PEPTIDE (1)..(12) Xaa at residues 5 and 8 is Glu orgamma-carboxy-Glu; Xaa at residue 7 is Trp (D or L) or bromo-Trp (D orL) 289 Gly Asn Cys Cys Xaa Phe Xaa Xaa Phe Cys Cys Asp 1 5 10 290 209DNA Conus dalli CDS (21)..(194) 290 ggaagctgac tacaagcaga atg cac tgtctc cca gtc ttc gtc att ctt ctg 53 Met His Cys Leu Pro Val Phe Val IleLeu Leu 1 5 10 ctg ctg act gca tct gga cct agc gtt gat gcc caa ccg aagacc gaa 101 Leu Leu Thr Ala Ser Gly Pro Ser Val Asp Ala Gln Pro Lys ThrGlu 15 20 25 gtt gat gtg ccc ctg tca tct ttc cgc gat aat gca aag cgt gcccta 149 Val Asp Val Pro Leu Ser Ser Phe Arg Asp Asn Ala Lys Arg Ala Leu30 35 40 caa aga ctt ccg cgt tgc tgt gaa tat tgg aag ttg tgc tgt ggt 194Gln Arg Leu Pro Arg Cys Cys Glu Tyr Trp Lys Leu Cys Cys Gly 45 50 55taaccagcat gaagg 209 291 58 PRT Conus dalli 291 Met His Cys Leu Pro ValPhe Val Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 Gly Pro Ser Val AspAla Gln Pro Lys Thr Glu Val Asp Val Pro Leu 20 25 30 Ser Ser Phe Arg AspAsn Ala Lys Arg Ala Leu Gln Arg Leu Pro Arg 35 40 45 Cys Cys Glu Tyr TrpLys Leu Cys Cys Gly 50 55 292 9 PRT Conus dalli PEPTIDE (1)..(9) Xaa atresidue 1 is Glu or gamma-carboxy-Glu; Xaa at residue 5 is Trp (D or L)or bromo-Trp (D or L); Xaa at residue 4 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 292 Cys Cys Xaa Xaa Xaa LysLeu Cys Cys 1 5 293 218 DNA Conus omaria CDS (21)..(203) 293 ggaagctgactacaagcaga atg cgc tgt ctc cca gtc ttc gtc att ctt ctg 53 Met Arg CysLeu Pro Val Phe Val Ile Leu Leu 1 5 10 ctg cta act gca tct gca cct agcgtt gat gcc cga ccg aag gcc aaa 101 Leu Leu Thr Ala Ser Ala Pro Ser ValAsp Ala Arg Pro Lys Ala Lys 15 20 25 gat gat gtg ccc ctg gca tct ttc cgtgat aat gca aag agt acc cta 149 Asp Asp Val Pro Leu Ala Ser Phe Arg AspAsn Ala Lys Ser Thr Leu 30 35 40 caa aga ctt cag gac aaa cgc gtt tgc tgtggc tat aag ttt ttt tgc 197 Gln Arg Leu Gln Asp Lys Arg Val Cys Cys GlyTyr Lys Phe Phe Cys 45 50 55 tgt cgt taaccagcat gaagg 218 Cys Arg 60 29461 PRT Conus omaria 294 Met Arg Cys Leu Pro Val Phe Val Ile Leu Leu LeuLeu Thr Ala Ser 1 5 10 15 Ala Pro Ser Val Asp Ala Arg Pro Lys Ala LysAsp Asp Val Pro Leu 20 25 30 Ala Ser Phe Arg Asp Asn Ala Lys Ser Thr LeuGln Arg Leu Gln Asp 35 40 45 Lys Arg Val Cys Cys Gly Tyr Lys Phe Phe CysCys Arg 50 55 60 295 11 PRT Conus omaria PEPTIDE (1)..(11) Xaa atresidue 5 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 295 Val Cys Cys Gly Xaa Lys Phe Phe Cys Cys Arg 1 5 10 296212 DNA Conus aulicus CDS (21)..(197) 296 ggaagctgac tacaagcaga atg cgctgt ctc cca gtc ttc gtc att ctt ctg 53 Met Arg Cys Leu Pro Val Phe ValIle Leu Leu 1 5 10 ctg ctg act gca tct gca cct aac gtt gat gcc caa ccgaag acc aaa 101 Leu Leu Thr Ala Ser Ala Pro Asn Val Asp Ala Gln Pro LysThr Lys 15 20 25 gat gat gtg ccc ctg gca tct ttg cac gat gat gca aag agtgca cta 149 Asp Asp Val Pro Leu Ala Ser Leu His Asp Asp Ala Lys Ser AlaLeu 30 35 40 caa cat tgg aac caa cgc tgc tgc ccc atg atc tat tgg tgc tgtagt 197 Gln His Trp Asn Gln Arg Cys Cys Pro Met Ile Tyr Trp Cys Cys Ser45 50 55 taaccagcat gaagg 212 297 59 PRT Conus aulicus 297 Met Arg CysLeu Pro Val Phe Val Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 Ala ProAsn Val Asp Ala Gln Pro Lys Thr Lys Asp Asp Val Pro Leu 20 25 30 Ala SerLeu His Asp Asp Ala Lys Ser Ala Leu Gln His Trp Asn Gln 35 40 45 Arg CysCys Pro Met Ile Tyr Trp Cys Cys Ser 50 55 298 10 PRT Conus aulicusPEPTIDE (1)..(10) Xaa at residue 3 is Pro or hydroxy-Pro; Xaa at residue7 is Trp (D or L) or bromo-Trp (D or L); Xaa at residue 6 is Tyr,125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 298Cys Cys Xaa Met Ile Xaa Xaa Cys Cys Ser 1 5 10 299 212 DNA Conus aulicusCDS (21)..(197) 299 ggaagctgac tacaagcaga atg cac tgt ctc cca gtc ttcgtc att ctt ctg 53 Met His Cys Leu Pro Val Phe Val Ile Leu Leu 1 5 10ctg ctg act gca tct gca cct aac gtt gat gcc caa ccg aag acc aaa 101 LeuLeu Thr Ala Ser Ala Pro Asn Val Asp Ala Gln Pro Lys Thr Lys 15 20 25 gatgat gtg ccc ctg gca tct ttg cac gat gat gca aag agt gca cta 149 Asp AspVal Pro Leu Ala Ser Leu His Asp Asp Ala Lys Ser Ala Leu 30 35 40 caa cattgg aac caa cgc tgc tgc ccc gag atc tat tgg tgc tgt agt 197 Gln His TrpAsn Gln Arg Cys Cys Pro Glu Ile Tyr Trp Cys Cys Ser 45 50 55 taaccagcatgaagg 212 300 59 PRT Conus aulicus 300 Met His Cys Leu Pro Val Phe ValIle Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 Ala Pro Asn Val Asp Ala GlnPro Lys Thr Lys Asp Asp Val Pro Leu 20 25 30 Ala Ser Leu His Asp Asp AlaLys Ser Ala Leu Gln His Trp Asn Gln 35 40 45 Arg Cys Cys Pro Glu Ile TyrTrp Cys Cys Ser 50 55 301 10 PRT Conus aulicus PEPTIDE (1)..(10) Xaa atresidue 4 is Glu or gamma-carboxy-Glu; Xaa at residue 3 is Pro orhydroxy-Pro; Xaa at residue 7 is Trp (D or L) or bromo- Trp (D or L);Xaa at residue 6 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr,O-sulpho-Tyr or O-phospho-Tyr 301 Cys Cys Xaa Xaa Ile Xaa Xaa Cys CysSer 1 5 10 302 215 DNA Conus ammiralis CDS (21)..(200) 302 ggaagctgactacaagcaga atg cgc tgt ctc cca gtc ttc gtc att ctt ctg 53 Met Arg CysLeu Pro Val Phe Val Ile Leu Leu 1 5 10 ctg ctg att gca tct gca cct agcgtt gat gcc caa ccg aag acc aaa 101 Leu Leu Ile Ala Ser Ala Pro Ser ValAsp Ala Gln Pro Lys Thr Lys 15 20 25 gat gat gtg tcc ctg gca tct ttg cacgat aat ata aag agt act cta 149 Asp Asp Val Ser Leu Ala Ser Leu His AspAsn Ile Lys Ser Thr Leu 30 35 40 caa aca ctt tgg aac aaa cgc tgc tgc ccccct gtg att tgg tgc tgt 197 Gln Thr Leu Trp Asn Lys Arg Cys Cys Pro ProVal Ile Trp Cys Cys 45 50 55 ggt taaccagcat aaagg 215 Gly 60 303 60 PRTConus ammiralis 303 Met Arg Cys Leu Pro Val Phe Val Ile Leu Leu Leu LeuIle Ala Ser 1 5 10 15 Ala Pro Ser Val Asp Ala Gln Pro Lys Thr Lys AspAsp Val Ser Leu 20 25 30 Ala Ser Leu His Asp Asn Ile Lys Ser Thr Leu GlnThr Leu Trp Asn 35 40 45 Lys Arg Cys Cys Pro Pro Val Ile Trp Cys Cys Gly50 55 60 304 9 PRT Conus ammiralis PEPTIDE (1)..(9) Xaa at residues 3and 4 is Pro or hydroxy-Pro; Xaa at residue 7 is Trp (D or L) orbromo-Trp (D or L) 304 Cys Cys Xaa Xaa Val Ile Xaa Cys Cys 1 5 305 215DNA Conus aulicus CDS (21)..(200) 305 ggaagctgac tacaagcaga atg cgc tgtctc cca gtc ttc gtc att ctt ctg 53 Met Arg Cys Leu Pro Val Phe Val IleLeu Leu 1 5 10 ctg ctg act gca tct gga cct agc gtt gat gcc cga ccg aagacc aaa 101 Leu Leu Thr Ala Ser Gly Pro Ser Val Asp Ala Arg Pro Lys ThrLys 15 20 25 gat gat gtg cct ctg tca tct ttc cgc gat aac gca aag agt atccta 149 Asp Asp Val Pro Leu Ser Ser Phe Arg Asp Asn Ala Lys Ser Ile Leu30 35 40 caa aga cgt tgg aac aac tat tgc tgc acg aat gag ctt tgg tgc tgt197 Gln Arg Arg Trp Asn Asn Tyr Cys Cys Thr Asn Glu Leu Trp Cys Cys 4550 55 ggt taaccagcat gaagg 215 Gly 60 306 60 PRT Conus aulicus 306 MetArg Cys Leu Pro Val Phe Val Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15Gly Pro Ser Val Asp Ala Arg Pro Lys Thr Lys Asp Asp Val Pro Leu 20 25 30Ser Ser Phe Arg Asp Asn Ala Lys Ser Ile Leu Gln Arg Arg Trp Asn 35 40 45Asn Tyr Cys Cys Thr Asn Glu Leu Trp Cys Cys Gly 50 55 60 307 13 PRTConus aulicus PEPTIDE (1)..(13) Xaa at residue 9 is Glu orgamma-carboxy-Glu; Xaa at residues 1 and 11 is Trp (D or L) or bromo-Trp(D or L); Xaa at residue 4 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr,O-sulpho-Tyr or O-phospho-Tyr 307 Xaa Asn Asn Xaa Cys Cys Thr Asn XaaLeu Xaa Cys Cys 1 5 10 308 218 DNA Conus dalli CDS (21)..(203) 308ggaagctgac tacaagcaga atg cac tgt ctc cca gtc ttc gtc att ctt ctg 53 MetHis Cys Leu Pro Val Phe Val Ile Leu Leu 1 5 10 ctg ctg act gca tct ggacct agc gtt gat gcc cga ccg aag acc gaa 101 Leu Leu Thr Ala Ser Gly ProSer Val Asp Ala Arg Pro Lys Thr Glu 15 20 25 gat gat gtg ccc ctg tca tctttc cgc gat aat aca aag agt acc cta 149 Asp Asp Val Pro Leu Ser Ser PheArg Asp Asn Thr Lys Ser Thr Leu 30 35 40 caa aga ctt ttg aag cca gtc aactgc tgt cct att gat caa tct tgc 197 Gln Arg Leu Leu Lys Pro Val Asn CysCys Pro Ile Asp Gln Ser Cys 45 50 55 tgt tct taaccagcat gaagg 218 CysSer 60 309 61 PRT Conus dalli 309 Met His Cys Leu Pro Val Phe Val IleLeu Leu Leu Leu Thr Ala Ser 1 5 10 15 Gly Pro Ser Val Asp Ala Arg ProLys Thr Glu Asp Asp Val Pro Leu 20 25 30 Ser Ser Phe Arg Asp Asn Thr LysSer Thr Leu Gln Arg Leu Leu Lys 35 40 45 Pro Val Asn Cys Cys Pro Ile AspGln Ser Cys Cys Ser 50 55 60 310 13 PRT Conus dalli PEPTIDE (1)..(13)Xaa at residues 1 and 6 is Pro or hydroxy-Pro 310 Xaa Val Asn Cys CysXaa Ile Asp Gln Ser Cys Cys Ser 1 5 10 311 239 DNA Conus consors CDS(7)..(228) 311 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtc ttg gca accact gtc 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val 1 510 gtt tcc atc cct tca gat cgt gca tct gaa ggc agg aat gcc gta gtc 96Val Ser Ile Pro Ser Asp Arg Ala Ser Glu Gly Arg Asn Ala Val Val 15 20 2530 cac gag aga gcg cct gag ctg gtc gtt acg gcc acc acg act tgc tgt 144His Glu Arg Ala Pro Glu Leu Val Val Thr Ala Thr Thr Thr Cys Cys 35 40 45ggt tat gat ccg atg aca ata tgc cct cct tgc atg tgc act cat tcc 192 GlyTyr Asp Pro Met Thr Ile Cys Pro Pro Cys Met Cys Thr His Ser 50 55 60 tgtcca cca aaa aga aaa cca ggc cgc aga aac gac tgatgctcga g 239 Cys Pro ProLys Arg Lys Pro Gly Arg Arg Asn Asp 65 70 312 74 PRT Conus consors 312Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val Val Ser 1 5 1015 Ile Pro Ser Asp Arg Ala Ser Glu Gly Arg Asn Ala Val Val His Glu 20 2530 Arg Ala Pro Glu Leu Val Val Thr Ala Thr Thr Thr Cys Cys Gly Tyr 35 4045 Asp Pro Met Thr Ile Cys Pro Pro Cys Met Cys Thr His Ser Cys Pro 50 5560 Pro Lys Arg Lys Pro Gly Arg Arg Asn Asp 65 70 313 36 PRT Conusconsors PEPTIDE (1)..(36) Xaa at residue 3 is Glu or gamma-carboxy-Glu;Xaa at residues 2, 17, 22, 23, 31, 32 and 36 is Pro or hydroxy-Pro; Xaaat residue 15 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyror O-phospho-Tyr 313 Ala Xaa Xaa Leu Val Val Thr Ala Thr Thr Thr Cys CysGly Xaa Asp 1 5 10 15 Xaa Met Thr Ile Cys Xaa Xaa Cys Met Cys Thr HisSer Cys Xaa Xaa 20 25 30 Lys Arg Lys Xaa 35 314 272 DNA Conus aurisiacusCDS (7)..(237) 314 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtc ttg gcaacc act gtc 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val 15 10 gtt tcc atc cct tca gat cgt gca tct gat ggc agg aat gcc gca gtc 96Val Ser Ile Pro Ser Asp Arg Ala Ser Asp Gly Arg Asn Ala Ala Val 15 20 2530 aac gag aga caa tct tgg ctg gtc cct tcg aca atc acg act tgc tgt 144Asn Glu Arg Gln Ser Trp Leu Val Pro Ser Thr Ile Thr Thr Cys Cys 35 40 45gga tat gat ccg ggg aca atg tgc cct cct tgc agg tgc aat aat acc 192 GlyTyr Asp Pro Gly Thr Met Cys Pro Pro Cys Arg Cys Asn Asn Thr 50 55 60 tgtaaa cca aaa aaa cca aaa cca gga aaa ggc cgc aga aac gac 237 Cys Lys ProLys Lys Pro Lys Pro Gly Lys Gly Arg Arg Asn Asp 65 70 75 tgatgctccaggaccctctg aaccacgacc tcgag 272 315 77 PRT Conus aurisiacus 315 Met PheThr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val Val Ser 1 5 10 15 IlePro Ser Asp Arg Ala Ser Asp Gly Arg Asn Ala Ala Val Asn Glu 20 25 30 ArgGln Ser Trp Leu Val Pro Ser Thr Ile Thr Thr Cys Cys Gly Tyr 35 40 45 AspPro Gly Thr Met Cys Pro Pro Cys Arg Cys Asn Asn Thr Cys Lys 50 55 60 ProLys Lys Pro Lys Pro Gly Lys Gly Arg Arg Asn Asp 65 70 75 316 39 PRTConus aurisiacus PEPTIDE (1)..(39) Xaa at residue 1 is Gln or pyro-Gln;Xaa at residues 6, 17, 22, 23, 32, 35 and 37 is Pro or hydroxy-Pro; Xaaat residue 3 is Trp (D or L) or bromo-Trp (D or L); Xaa at residue 15 isTyr, 125I-Tyr , mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 316 Xaa Ser Xaa Leu Val Xaa Ser Thr Ile Thr Thr Cys CysGly Xaa Asp 1 5 10 15 Xaa Gly Thr Met Cys Xaa Xaa Cys Arg Cys Asn AsnThr Cys Lys Xaa 20 25 30 Lys Lys Xaa Lys Xaa Gly Lys 35 317 266 DNAConus consors CDS (7)..(231) 317 ggatcc atg ttc acc gtg ttt ctg ttg gttgtc ttg gca acc act gtc 48 Met Phe Thr Val Phe Leu Leu Val Val Leu AlaThr Thr Val 1 5 10 gtt tcc atc cct tca gat cgt gca tct gat ggc agg aatgcc gta gtc 96 Val Ser Ile Pro Ser Asp Arg Ala Ser Asp Gly Arg Asn AlaVal Val 15 20 25 30 cac gag aga gcg cct gag ctg gtc gtt acg gcc acc acgact tgc tgt 144 His Glu Arg Ala Pro Glu Leu Val Val Thr Ala Thr Thr ThrCys Cys 35 40 45 ggt tat gat ccg atg aca tgg tgc cct tct tgc atg tgc acttat tcc 192 Gly Tyr Asp Pro Met Thr Trp Cys Pro Ser Cys Met Cys Thr TyrSer 50 55 60 tgt ccc cac caa agg aaa aaa cca ggc cgc aga aac gactgatgctcca 241 Cys Pro His Gln Arg Lys Lys Pro Gly Arg Arg Asn Asp 65 7075 ggaccctctg aaccacgacc tcgag 266 318 75 PRT Conus consors 318 Met PheThr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val Val Ser 1 5 10 15 IlePro Ser Asp Arg Ala Ser Asp Gly Arg Asn Ala Val Val His Glu 20 25 30 ArgAla Pro Glu Leu Val Val Thr Ala Thr Thr Thr Cys Cys Gly Tyr 35 40 45 AspPro Met Thr Trp Cys Pro Ser Cys Met Cys Thr Tyr Ser Cys Pro 50 55 60 HisGln Arg Lys Lys Pro Gly Arg Arg Asn Asp 65 70 75 319 37 PRT Conusconsors PEPTIDE (1)..(37) Xaa at residue 3 is Glu or gamma-carboxy-Glu;Xaa at residues 2, 17, 22, 31 and 37 is Pro or hydroxy-Pro; Xaa atresidue 20 is Trp (D or L) or bromo-Trp (D or L); 319 Ala Xaa Xaa LeuVal Val Thr Ala Thr Thr Thr Cys Cys Gly Xaa Asp 1 5 10 15 Xaa Met ThrXaa Cys Xaa Ser Cys Met Cys Thr Xaa Ser Cys Xaa His 20 25 30 Gln Arg LysLys Xaa 35 320 260 DNA Conus magus CDS (7)..(231) misc_feature(1)..(260) n is unknown 320 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtcttg gca acc agt gtc 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala ThrSer Val 1 5 10 gtt tcc atc cct tca gat cgt gca tct gat ggc ggg aat gccgta gtc 96 Val Ser Ile Pro Ser Asp Arg Ala Ser Asp Gly Gly Asn Ala ValVal 15 20 25 30 cac gag aga gcg cct gag ctg gtc gtt acg gcc acc acg acttgc tgt 144 His Glu Arg Ala Pro Glu Leu Val Val Thr Ala Thr Thr Thr CysCys 35 40 45 ggt tat gat ccg atg aca ata tgc cct ccc tgc atg tgc act cattcc 192 Gly Tyr Asp Pro Met Thr Ile Cys Pro Pro Cys Met Cys Thr His Ser50 55 60 tgt cca cca aaa gga aaa cca ggc cgc agg aac gac tga tgtccaggac241 Cys Pro Pro Lys Gly Lys Pro Gly Arg Arg Asn Asp 65 70 ctctgaaccacgacncgag 260 321 74 PRT Conus magus 321 Met Phe Thr Val Phe Leu Leu ValVal Leu Ala Thr Ser Val Val Ser 1 5 10 15 Ile Pro Ser Asp Arg Ala SerAsp Gly Gly Asn Ala Val Val His Glu 20 25 30 Arg Ala Pro Glu Leu Val ValThr Ala Thr Thr Thr Cys Cys Gly Tyr 35 40 45 Asp Pro Met Thr Ile Cys ProPro Cys Met Cys Thr His Ser Cys Pro 50 55 60 Pro Lys Gly Lys Pro Gly ArgArg Asn Asp 65 70 322 36 PRT Conus magus PEPTIDE (1)..(36) Xaa atresidue 3 is Glu or gamma-carboxy-Glu; Xaa at residues 2, 17, 22, 23,31, 32 and 36 is Pro or hydroxy-Pro; Xaa at residue 15 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 322 Ala XaaXaa Leu Val Val Thr Ala Thr Thr Thr Cys Cys Gly Xaa Asp 1 5 10 15 XaaMet Thr Ile Cys Xaa Xaa Cys Met Cys Thr His Ser Cys Xaa Xaa 20 25 30 LysGly Lys Xaa 35 323 251 DNA Conus aurisiacus CDS (23)..(202) 323gaattcgccc ttgaggatcc gt gtg gtt ctg ggt cca gaa cct gat ggc agg 52 ValVal Leu Gly Pro Glu Pro Asp Gly Arg 1 5 10 aat gcc gca gtc aac gag agacag aaa tgg ctg gtc cat tcg aaa atc 100 Asn Ala Ala Val Asn Glu Arg GlnLys Trp Leu Val His Ser Lys Ile 15 20 25 acg tat tgc tgt ggt tat aat aagatg gac atg tgc cct cct tgc atg 148 Thr Tyr Cys Cys Gly Tyr Asn Lys MetAsp Met Cys Pro Pro Cys Met 30 35 40 tgc act tat tcc tgt ccc ccc cta aaaaaa aaa aga cca ggc cgc aga 196 Cys Thr Tyr Ser Cys Pro Pro Leu Lys LysLys Arg Pro Gly Arg Arg 45 50 55 aac gac tgatgctcca ggaccctctgaaccacgacc tcgagcgaag ggcgaattc 251 Asn Asp 60 324 60 PRT Conusaurisiacus 324 Val Val Leu Gly Pro Glu Pro Asp Gly Arg Asn Ala Ala ValAsn Glu 1 5 10 15 Arg Gln Lys Trp Leu Val His Ser Lys Ile Thr Tyr CysCys Gly Tyr 20 25 30 Asn Lys Met Asp Met Cys Pro Pro Cys Met Cys Thr TyrSer Cys Pro 35 40 45 Pro Leu Lys Lys Lys Arg Pro Gly Arg Arg Asn Asp 5055 60 325 38 PRT Conus aurisiacus PEPTIDE (1)..(38) Xaa at residue 1 isGln or pyro-Gln; Xaa at residues 22, 23, 31, 32 and 38 is Pro orhydroxy-Pro; Xaa at residue 3 is Trp (D or L) or bromo-Trp (D or L); Xaaat residues 11, 15 and 28 is Tyr, 125I -Tyr, mono-iodo-Tyr, di-iodo-Tyr,O-sulpho-Tyr or O-phospho-Tyr 325 Xaa Lys Xaa Leu Val His Ser Lys IleThr Xaa Cys Cys Gly Xaa Asn 1 5 10 15 Lys Met Asp Met Cys Xaa Xaa CysMet Cys Thr Xaa Ser Cys Xaa Xaa 20 25 30 Leu Lys Lys Lys Arg Xaa 35 326212 DNA Conus aurisiacus CDS (23)..(163) 326 gaattcgccc ttgaggatcc gtgtg gtt ctg ggt cca gca ttt gat ggc agg 52 Val Val Leu Gly Pro Ala PheAsp Gly Arg 1 5 10 aat gcc gca gtc aac gag aga gcg cct tgg acg gtc gttacg gcc acc 100 Asn Ala Ala Val Asn Glu Arg Ala Pro Trp Thr Val Val ThrAla Thr 15 20 25 acg aat tgc tgc ggt att acc ggg cca ggc tgc ctt cct tgccgt tgt 148 Thr Asn Cys Cys Gly Ile Thr Gly Pro Gly Cys Leu Pro Cys ArgCys 30 35 40 act caa aca tgt ggc tgatgctcca ggaccctctg aaccacgacctcgagcgaag 203 Thr Gln Thr Cys Gly 45 ggcgaattc 212 327 47 PRT Conusaurisiacus 327 Val Val Leu Gly Pro Ala Phe Asp Gly Arg Asn Ala Ala ValAsn Glu 1 5 10 15 Arg Ala Pro Trp Thr Val Val Thr Ala Thr Thr Asn CysCys Gly Ile 20 25 30 Thr Gly Pro Gly Cys Leu Pro Cys Arg Cys Thr Gln ThrCys Gly 35 40 45 328 29 PRT Conus aurisiacus PEPTIDE (1)..(29) Xaa atresidues 2, 18 and 22 is Pro or hydroxy-Pro; Xaa at residue 3 is Trp (Dor L) or bromo-Trp (D or L) 328 Ala Xaa Xaa Thr Val Val Thr Ala Thr ThrAsn Cys Cys Gly Ile Thr 1 5 10 15 Gly Xaa Gly Cys Leu Xaa Cys Arg CysThr Gln Thr Cys 20 25 329 218 DNA Conus marmoreus CDS (21)..(203) 329ggaagctgac tacaagcaga atg cgc tgt ctc cca gtc ttg atc att ctt ctg 53 MetArg Cys Leu Pro Val Leu Ile Ile Leu Leu 1 5 10 ctg ctg act gca tct gcacct ggc gtt gtt gtc cta ccg aag acc gaa 101 Leu Leu Thr Ala Ser Ala ProGly Val Val Val Leu Pro Lys Thr Glu 15 20 25 gat gat gtg ccc atg tca tctgtc tac ggt aat gga aag agt atc cta 149 Asp Asp Val Pro Met Ser Ser ValTyr Gly Asn Gly Lys Ser Ile Leu 30 35 40 cga ggg att ctg agg aac ggt gtttgc tgt ggc tat aag ttg tgc ctt 197 Arg Gly Ile Leu Arg Asn Gly Val CysCys Gly Tyr Lys Leu Cys Leu 45 50 55 cca tgt taaccagcat gaagg 218 ProCys 60 330 61 PRT Conus marmoreus 330 Met Arg Cys Leu Pro Val Leu IleIle Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 Ala Pro Gly Val Val Val LeuPro Lys Thr Glu Asp Asp Val Pro Met 20 25 30 Ser Ser Val Tyr Gly Asn GlyLys Ser Ile Leu Arg Gly Ile Leu Arg 35 40 45 Asn Gly Val Cys Cys Gly TyrLys Leu Cys Leu Pro Cys 50 55 60 331 13 PRT Conus marmoreus PEPTIDE(1)..(13) Xaa at residue 12 is Pro or hydroxy-Pro; Xaa at residue 7 isTyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr331 Asn Gly Val Cys Cys Gly Xaa Lys Leu Cys Leu Xaa Cys 1 5 10 332 238DNA Conus pennaceus CDS (28)..(219) 332 ggaattcgga agctgactac aagcagaatg cgc tgt ctc cca gtc ttc gtc att 54 Met Arg Cys Leu Pro Val Phe ValIle 1 5 ctt ctg ctg ctg act gca tct gca cct agc gtt gat gcc aaa gtt cat102 Leu Leu Leu Leu Thr Ala Ser Ala Pro Ser Val Asp Ala Lys Val His 1015 20 25 ctg aag acc aaa ggt gat ggg ccc ctg tca tct ttc cga gat aat gca150 Leu Lys Thr Lys Gly Asp Gly Pro Leu Ser Ser Phe Arg Asp Asn Ala 3035 40 aag agt acc cta caa aga ctt cag gac aaa agc act tgc tgt ggc ttt198 Lys Ser Thr Leu Gln Arg Leu Gln Asp Lys Ser Thr Cys Cys Gly Phe 4550 55 aag atg tgt atc cct tgt agt taaccagcat gaaggatcc 238 Lys Met CysIle Pro Cys Ser 60 333 64 PRT Conus pennaceus 333 Met Arg Cys Leu ProVal Phe Val Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 Ala Pro Ser ValAsp Ala Lys Val His Leu Lys Thr Lys Gly Asp Gly 20 25 30 Pro Leu Ser SerPhe Arg Asp Asn Ala Lys Ser Thr Leu Gln Arg Leu 35 40 45 Gln Asp Lys SerThr Cys Cys Gly Phe Lys Met Cys Ile Pro Cys Ser 50 55 60 334 13 PRTConus pennaceus PEPTIDE (1)..(13) Xaa at residue 11 is Pro orhydroxy-Pro 334 Ser Thr Cys Cys Gly Phe Lys Met Cys Ile Xaa Cys Ser 1 510 335 231 DNA Conus pennaceus CDS (27)..(212) 335 gaattcggaa gctgactacaagcaga atg cgt tgt ctc cca gtc ttc gtc att 53 Met Arg Cys Leu Pro ValPhe Val Ile 1 5 ctt ctg ctg ctg act gca tct gga cct agc gtt gat gcc cgactg aag 101 Leu Leu Leu Leu Thr Ala Ser Gly Pro Ser Val Asp Ala Arg LeuLys 10 15 20 25 acc aaa gat gat gtg ccc ctg tca tct ttc cga gat aat gcaaag agt 149 Thr Lys Asp Asp Val Pro Leu Ser Ser Phe Arg Asp Asn Ala LysSer 30 35 40 acc cta caa aga ctt cag gac aaa cgc ctt tgc tgt ggc ttt tggatg 197 Thr Leu Gln Arg Leu Gln Asp Lys Arg Leu Cys Cys Gly Phe Trp Met45 50 55 tgt att cct tgt aat taaccagcat gaaggatcc 231 Cys Ile Pro CysAsn 60 336 62 PRT Conus pennaceus 336 Met Arg Cys Leu Pro Val Phe ValIle Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 Gly Pro Ser Val Asp Ala ArgLeu Lys Thr Lys Asp Asp Val Pro Leu 20 25 30 Ser Ser Phe Arg Asp Asn AlaLys Ser Thr Leu Gln Arg Leu Gln Asp 35 40 45 Lys Arg Leu Cys Cys Gly PheTrp Met Cys Ile Pro Cys Asn 50 55 60 337 12 PRT Conus pennaceus PEPTIDE(1)..(12) Xaa at residue 10 is Pro or hydroxy-Pro; Xaa at residue 6 isTrp (D or L) or bromo-Trp (D or L) 337 Leu Cys Cys Gly Phe Xaa Met CysIle Xaa Cys Asn 1 5 10 338 244 DNA Conus pennaceus CDS (40)..(225)misc_feature (1)..(244) n is unknown 338 gaattctccc ttggaattctgaagctgact acaancaga atg cgt tgt ctc cca 54 Met Arg Cys Leu Pro 1 5 ctcttc gtc att ctt ctg ctg ctg act gca tct gga cct act gtt gat 102 Leu PheVal Ile Leu Leu Leu Leu Thr Ala Ser Gly Pro Thr Val Asp 10 15 20 gcc cgactg aag acc aaa gat gat gtg ccc ctg tca tct ttc cga gat 150 Ala Arg LeuLys Thr Lys Asp Asp Val Pro Leu Ser Ser Phe Arg Asp 25 30 35 aat gca aagagt acc cta caa aga ctt cag gac aaa agc act tgc tgt 198 Asn Ala Lys SerThr Leu Gln Arg Leu Gln Asp Lys Ser Thr Cys Cys 40 45 50 ggc ttt aag atgtgt att cct tgt ggt taaccagcat gaaggatcc 244 Gly Phe Lys Met Cys Ile ProCys Gly 55 60 339 62 PRT Conus pennaceus 339 Met Arg Cys Leu Pro Leu PheVal Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 Gly Pro Thr Val Asp AlaArg Leu Lys Thr Lys Asp Asp Val Pro Leu 20 25 30 Ser Ser Phe Arg Asp AsnAla Lys Ser Thr Leu Gln Arg Leu Gln Asp 35 40 45 Lys Ser Thr Cys Cys GlyPhe Lys Met Cys Ile Pro Cys Gly 50 55 60 340 12 PRT Conus pennaceusPEPTIDE (1)..(12) Xaa at residue 11 is Pro or hydroxy-Pro 340 Ser ThrCys Cys Gly Phe Lys Met Cys Ile Xaa Cys 1 5 10 341 250 DNA Conusepiscopatus misc_feature (1)..(250) n is unknown 341 gaattcgcccttggaattcg gaagctgact acaagcagaa tgcgctgtct cccagtcttc 60 gtcattcttctgctgctgac tgcatctgga cctantgttg atgccaaagt tcatctgaag 120 accaaaggtgatgggcccct gtcatctttc cgagataatg caaagagtac cctacaaaga 180 cttcaggacaaaagcacttg ctgtggctat aggatgtgtg ttccttgtgg ttaaccagca 240 tgaaggatcc250 342 64 PRT Conus episcopatus 342 Met Arg Cys Leu Pro Val Phe Val IleLeu Leu Leu Leu Thr Ala Ser 1 5 10 15 Gly Pro Ser Val Asp Ala Lys ValHis Leu Lys Thr Lys Gly Asp Gly 20 25 30 Pro Leu Ser Ser Phe Arg Asp AsnAla Lys Ser Thr Leu Gln Arg Leu 35 40 45 Gln Asp Lys Ser Thr Cys Cys GlyTyr Arg Met Cys Val Pro Cys Gly 50 55 60 343 12 PRT Conus episcopatusPEPTIDE (1)..(12) Xaa at residue 11 is Pro or hydroxy-Pro; Xaa atresidue 6 is Tyr , 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 343 Ser Thr Cys Cys Gly Xaa Arg Met Cys Val Xaa Cys 1 5 10344 827 DNA Conus marmoreus CDS (82)..(264) 344 ggcgaataca cctggcaggtactcaacgaa cttcaggaca cattcttttc acctggacac 60 tggaaactga caacaggcag aatg cgc tgt ctc cca gtc ttg atc att ctt 111 Met Arg Cys Leu Pro Val LeuIle Ile Leu 1 5 10 ctg ctg ctg act gca tct gca cct ggc gtt gtt gtc ctaccg aag acc 159 Leu Leu Leu Thr Ala Ser Ala Pro Gly Val Val Val Leu ProLys Thr 15 20 25 gaa gat gat gtg ccc atg tca tct gtc tac ggt aat gga aagagt atc 207 Glu Asp Asp Val Pro Met Ser Ser Val Tyr Gly Asn Gly Lys SerIle 30 35 40 cta cga gga att ctg agg aac ggt gtt tgc tgt ggc tat aag ttgtgc 255 Leu Arg Gly Ile Leu Arg Asn Gly Val Cys Cys Gly Tyr Lys Leu Cys45 50 55 cat cca tgt taaccagcat gaagggaaat gactttggat gagacccctg 304 HisPro Cys 60 cgaactgtcc ctggatgtga aatttggaaa gcagactgtt cctttcgcacgtattcgtgg 364 aatttcgaat ggtcgtaaac aacacgctgc cacttgcagg ctactatctctctgtccttt 424 catctgtgga aatggatgat ctaacaactg aaatatcaga aatttttcaatggctataca 484 ctatgaccat gtagtcagta attatatcat ttggaccttt tgaaatatttttcaatatgt 544 aaagtttttg caccctggaa aggtcttttg gagttaaata ttttagtatgttatgttttg 604 catacaagtt atagaatgct gtctttcttt ttgttcccac atcaatggtgggggcagaaa 664 ttatttgttt tggtcaatgt aattatgacc tgcatttagt gctatagtgattgcattttc 724 agcgtggaat gtttaatctg caaacagaaa gtggttgatc gactaataaagatttgcatg 784 gcacaaaaaa aaaaaaaaaa agtactctgc gttgttactc gag 827 34561 PRT Conus marmoreus 345 Met Arg Cys Leu Pro Val Leu Ile Ile Leu LeuLeu Leu Thr Ala Ser 1 5 10 15 Ala Pro Gly Val Val Val Leu Pro Lys ThrGlu Asp Asp Val Pro Met 20 25 30 Ser Ser Val Tyr Gly Asn Gly Lys Ser IleLeu Arg Gly Ile Leu Arg 35 40 45 Asn Gly Val Cys Cys Gly Tyr Lys Leu CysHis Pro Cys 50 55 60 346 13 PRT Conus marmoreus PEPTIDE (1)..(13) Xaa atresidue 12 is Pro or hydroxy-Pro; Xaa at residue 7 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 346 Asn GlyVal Cys Cys Gly Xaa Lys Leu Cys His Xaa Cys 1 5 10 347 12 PRT Conusmarmoreus PEPTIDE (1)..(12) Xaa at residue 11 is Pro or hydroxy-Pro; Xaaat residue 6 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyror O-phospho-Tyr 347 Gly Val Cys Cys Gly Xaa Lys Leu Cys His Xaa Cys 1 510 348 202 DNA Conus bandanus CDS (1)..(183) 348 atg cgc tgt ctc cca gtcttg atc att ctt ctg ctg ctg act gca tct 48 Met Arg Cys Leu Pro Val LeuIle Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 gca cct ggc gtt gat gtccta ccg aag acc gaa gat gat gtg ccc ctg 96 Ala Pro Gly Val Asp Val LeuPro Lys Thr Glu Asp Asp Val Pro Leu 20 25 30 tca tct gtc tac gat aat acaaag agt atc cta cga gga ctt ctg gac 144 Ser Ser Val Tyr Asp Asn Thr LysSer Ile Leu Arg Gly Leu Leu Asp 35 40 45 aaa cgt gct tgc tgt ggc tac aagctt tgc tca cca tgt taaccagcat 193 Lys Arg Ala Cys Cys Gly Tyr Lys LeuCys Ser Pro Cys 50 55 60 gaaggatcc 202 349 61 PRT Conus bandanus 349 MetArg Cys Leu Pro Val Leu Ile Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15Ala Pro Gly Val Asp Val Leu Pro Lys Thr Glu Asp Asp Val Pro Leu 20 25 30Ser Ser Val Tyr Asp Asn Thr Lys Ser Ile Leu Arg Gly Leu Leu Asp 35 40 45Lys Arg Ala Cys Cys Gly Tyr Lys Leu Cys Ser Pro Cys 50 55 60 350 11 PRTConus bandanus PEPTIDE (1)..(11) Xaa at residue 10 is Pro orhydroxy-Pro; Xaa at residue 5 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 350 Ala Cys Cys Gly Xaa LysLeu Cys Ser Xaa Cys 1 5 10 351 221 DNA Conus aulicus CDS (21)..(206) 351ggaagctgac tacaagcaga atg cgc tgt ctc cca gtc ttc gtc att ctt ctg 53 MetArg Cys Leu Pro Val Phe Val Ile Leu Leu 1 5 10 ctg ctg act gca tct ggacct agc gtt gat gcc cga ctg aag acc aaa 101 Leu Leu Thr Ala Ser Gly ProSer Val Asp Ala Arg Leu Lys Thr Lys 15 20 25 gat gat gtg ccc ctg tca tctttc cga gat aat gca aag agt acc cta 149 Asp Asp Val Pro Leu Ser Ser PheArg Asp Asn Ala Lys Ser Thr Leu 30 35 40 caa aga cat cag gac aaa agc gtttgc tgt ggc tat aag ctg tgt ttt 197 Gln Arg His Gln Asp Lys Ser Val CysCys Gly Tyr Lys Leu Cys Phe 45 50 55 cct tgt ggt taaccagcat gaagg 221Pro Cys Gly 60 352 62 PRT Conus aulicus 352 Met Arg Cys Leu Pro Val PheVal Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 Gly Pro Ser Val Asp AlaArg Leu Lys Thr Lys Asp Asp Val Pro Leu 20 25 30 Ser Ser Phe Arg Asp AsnAla Lys Ser Thr Leu Gln Arg His Gln Asp 35 40 45 Lys Ser Val Cys Cys GlyTyr Lys Leu Cys Phe Pro Cys Gly 50 55 60 353 12 PRT Conus aulicusPEPTIDE (1)..(12) Xaa at residue 11 is Pro or hydroxy-Pro; Xaa atresidue 6 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 353 Ser Val Cys Cys Gly Xaa Lys Leu Cys Phe Xaa Cys 1 5 10354 312 DNA Conus textile CDS (3)..(50) misc_feature (1)..(312) n isunknown 354 ca gga tcc aat ggg gtt tgt tgt ggc tat agg atg tgt gtt ccttgt 47 Gly Ser Asn Gly Val Cys Cys Gly Tyr Arg Met Cys Val Pro Cys 1 510 15 ggt taaccagcat gaagggaaat gactttggat gagacccctg cgaactgtcc 100 Glyctggatgtga gatttggaaa gcagactgtt cattttgcac gtgttcgtgg aatttcgaat 160ggtcgttaac aacacgctgc cacttgcaag ctactatctc tctgtccttt tatctgtgga 220actgtatgat ctaacaactg aaatatcata nanatttttc aatgggtatn cactatgcat 280atgatcatgt agggttcaag gggtcaagat nc 312 355 16 PRT Conus textile 355 GlySer Asn Gly Val Cys Cys Gly Tyr Arg Met Cys Val Pro Cys Gly 1 5 10 15356 13 PRT Conus textile PEPTIDE (1)..(13) Xaa at residue 12 is Pro orhydroxy-Pro; Xaa at residue 7 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 356 Asn Gly Val Cys Cys GlyXaa Arg Met Cys Val Xaa Cys 1 5 10 357 205 DNA Conus textile CDS(1)..(186) 357 atg cac tgt ctc cca atc ttc gtc att ctt ctg ctg ctg actgca tct 48 Met His Cys Leu Pro Ile Phe Val Ile Leu Leu Leu Leu Thr AlaSer 1 5 10 15 gga cct agc gtt gat gcc caa ctg aag acc aaa gat gat gtgccc ctg 96 Gly Pro Ser Val Asp Ala Gln Leu Lys Thr Lys Asp Asp Val ProLeu 20 25 30 tca tct ttc cga gat cat gca aag agt acc cta cga aga ctt caggac 144 Ser Ser Phe Arg Asp His Ala Lys Ser Thr Leu Arg Arg Leu Gln Asp35 40 45 aaa cag act tgc tgt ggc tat agg atg tgt gtt cct tgt ggt 186 LysGln Thr Cys Cys Gly Tyr Arg Met Cys Val Pro Cys Gly 50 55 60 taaccagcatgaaggatcc 205 358 62 PRT Conus textile 358 Met His Cys Leu Pro Ile PheVal Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 Gly Pro Ser Val Asp AlaGln Leu Lys Thr Lys Asp Asp Val Pro Leu 20 25 30 Ser Ser Phe Arg Asp HisAla Lys Ser Thr Leu Arg Arg Leu Gln Asp 35 40 45 Lys Gln Thr Cys Cys GlyTyr Arg Met Cys Val Pro Cys Gly 50 55 60 359 12 PRT Conus textilePEPTIDE (1)..(12) Xaa at residue 1 is Gln or pyro-Gln; Xaa at residue 11is Pro or hydroxy-Pro; Xaa at residue 6 is Tyr, 125I-Tyr, mono-iodo-Tyr,di--iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 359 Xaa Thr Cys Cys Gly XaaArg Met Cys Val Xaa Cys 1 5 10 360 221 DNA Conus ammiralis CDS(21)..(206) 360 agaagctgac tacaagcaga atg cac tac ctc cca gtc ttc gtcatt ctt ctg 53 Met His Tyr Leu Pro Val Phe Val Ile Leu Leu 1 5 10 ctgctg act gca tct gga cct agc gtt gat gcc caa ctg aag acc aaa 101 Leu LeuThr Ala Ser Gly Pro Ser Val Asp Ala Gln Leu Lys Thr Lys 15 20 25 gat gatgtg ccc ctg tca tct ttc cga gat aat gca aag agt acc cta 149 Asp Asp ValPro Leu Ser Ser Phe Arg Asp Asn Ala Lys Ser Thr Leu 30 35 40 cga aga ctccag tac aaa cag gct tgc tgt ggc ttt aag atg tgt gtt 197 Arg Arg Leu GlnTyr Lys Gln Ala Cys Cys Gly Phe Lys Met Cys Val 45 50 55 cct tgt ggttaaccagcat gaagg 221 Pro Cys Gly 60 361 62 PRT Conus ammiralis 361 MetHis Tyr Leu Pro Val Phe Val Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15Gly Pro Ser Val Asp Ala Gln Leu Lys Thr Lys Asp Asp Val Pro Leu 20 25 30Ser Ser Phe Arg Asp Asn Ala Lys Ser Thr Leu Arg Arg Leu Gln Tyr 35 40 45Lys Gln Ala Cys Cys Gly Phe Lys Met Cys Val Pro Cys Gly 50 55 60 362 12PRT Conus ammiralis PEPTIDE (1)..(12) Xaa at residue 1 is Gln orpyro-Gln; Xaa at residue 11 is Pro or hydroxy-Pro 362 Xaa Ala Cys CysGly Phe Lys Met Cys Val Xaa Cys 1 5 10 363 211 DNA Conus pennaceus CDS(1)..(192) 363 atg cgc tgt ctc cca gtc ttc gtc att ctt ctg ctg ctg actgca tct 48 Met Arg Cys Leu Pro Val Phe Val Ile Leu Leu Leu Leu Thr AlaSer 1 5 10 15 gca cct agc gtt gat gcc aaa gtt cat ctg aag acc aaa ggtgat ggg 96 Ala Pro Ser Val Asp Ala Lys Val His Leu Lys Thr Lys Gly AspGly 20 25 30 ccc ctg tca tct ttc cga gat aat gca aag agt acc cta caa agactt 144 Pro Leu Ser Ser Phe Arg Asp Asn Ala Lys Ser Thr Leu Gln Arg Leu35 40 45 cag gac aaa agc act tgc tgt ggc ttt aag atg tgt att cct tgt cgt192 Gln Asp Lys Ser Thr Cys Cys Gly Phe Lys Met Cys Ile Pro Cys Arg 5055 60 taaccagcat gaaggatcc 211 364 64 PRT Conus pennaceus 364 Met ArgCys Leu Pro Val Phe Val Ile Leu Leu Leu Leu Thr Ala Ser 1 5 10 15 AlaPro Ser Val Asp Ala Lys Val His Leu Lys Thr Lys Gly Asp Gly 20 25 30 ProLeu Ser Ser Phe Arg Asp Asn Ala Lys Ser Thr Leu Gln Arg Leu 35 40 45 GlnAsp Lys Ser Thr Cys Cys Gly Phe Lys Met Cys Ile Pro Cys Arg 50 55 60 36513 PRT Conus pennaceus PEPTIDE (1)..(13) Xaa at residue 13 is Pro orhydroxy-Pro 365 Ser Thr Cys Cys Gly Phe Lys Met Cys Ile Xaa Cys Arg 1 510 366 304 DNA Conus pennaceus CDS (3)..(50) misc_feature (1)..(304) nis unknown 366 ca gga tcc aat ggg gtt tgt tgt ggc ttt tgg atg tgt attcct tgt 47 Gly Ser Asn Gly Val Cys Cys Gly Phe Trp Met Cys Ile Pro Cys 15 10 15 aat taaccagcat gaagggaaat gactttggat aagacccctg cgaactgtcc 100Asn ttggatgtga gatttggaaa gcagactgtt ccttttgcac gtgttcgtgg aatttcgaat160 ggtcgttaac aacacgctgc cacttgcaag ctactatctc tctgtccttt catctgtgga220 actgtatgat ctaacaactg aaatatcata gaaatttttc aatgggtata cactatgcat280 atgaccatgt angggtcaac agnc 304 367 16 PRT Conus pennaceus 367 GlySer Asn Gly Val Cys Cys Gly Phe Trp Met Cys Ile Pro Cys Asn 1 5 10 15368 14 PRT Conus pennaceus PEPTIDE (1)..(14) Xaa at residue 12 is Pro orhydroxy-Pro; Xaa at residue 8 is Trp (D or L) or bromo-Trp (D or L) 368Asn Gly Val Cys Cys Gly Phe Xaa Met Cys Ile Xaa Cys Asn 1 5 10 369 218DNA Conus omaria CDS (21)..(203) 369 ggaagctgac tacaagcaga atg cgc tgtctc cca gtc ttc gtc att ctt ctg 53 Met Arg Cys Leu Pro Val Phe Val IleLeu Leu 1 5 10 ctg ctg act gca tct gca cct agc gtt gat gcc cga ccg aaggcc aaa 101 Leu Leu Thr Ala Ser Ala Pro Ser Val Asp Ala Arg Pro Lys AlaLys 15 20 25 gat gat gtg ccc ctg tca tct ttc cgt gat aat gca aag agt acccta 149 Asp Asp Val Pro Leu Ser Ser Phe Arg Asp Asn Ala Lys Ser Thr Leu30 35 40 caa aga ctt cag gac aaa gac gtt tgc tgt tac gtt aga atg tgt cct197 Gln Arg Leu Gln Asp Lys Asp Val Cys Cys Tyr Val Arg Met Cys Pro 4550 55 tgt cgt taaccagcat gaagg 218 Cys Arg 60 370 61 PRT Conus omaria370 Met Arg Cys Leu Pro Val Phe Val Ile Leu Leu Leu Leu Thr Ala Ser 1 510 15 Ala Pro Ser Val Asp Ala Arg Pro Lys Ala Lys Asp Asp Val Pro Leu 2025 30 Ser Ser Phe Arg Asp Asn Ala Lys Ser Thr Leu Gln Arg Leu Gln Asp 3540 45 Lys Asp Val Cys Cys Tyr Val Arg Met Cys Pro Cys Arg 50 55 60 37112 PRT Conus omaria PEPTIDE (1)..(12) Xaa at residue 10 is Pro orhydroxy-Pro; Xaa at residue 5 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 371 Asp Val Cys Cys Xaa ValArg Met Cys Xaa Cys Arg 1 5 10 372 84 PRT Conus radiatus PEPTIDE(1)..(84) Xaa at residues 27, 32, 38, 39 and 41 is Glu orgamma-carboxy-Glu ; Xaa at residues 2, 5, 17, 23, 43, 45 and 58 is Proor hydroxy- Pro;Xaa at residue 8 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo- Tyr, O-sulpho-Tyr or O-phospho-Tyr 372 His Xaa Thr Lys Xaa CysMet Xaa Cys Ser Phe Gly Gln Cys Val Gly 1 5 10 15 Xaa His Ile Cys CysGly Xaa Thr Gly Cys Xaa Met Gly Thr Ala Xaa 20 25 30 Ala Asn Met Cys SerXaa Xaa Asp Xaa Asp Xaa Ile Xaa Cys Gln Val 35 40 45 Phe Gly Ser Asp CysAla Leu Asn Asn Xaa Asp Asn Ile His Gly His 50 55 60 Cys Val Ala Asp GlyIle Cys Cys Val Asp Asp Thr Cys Thr Thr His 65 70 75 80 Leu Gly Cys Leu373 218 DNA Conus tessulatus CDS (7)..(174) 373 ggatcc atg ttc acc gtgttt ctg ttg gtt gtc ttg gca acc act gtt 48 Met Phe Thr Val Phe Leu LeuVal Val Leu Ala Thr Thr Val 1 5 10 gtt tcc ttc agt gca gat cgt gcc aacgtc aaa gcg tct gac ctg atc 96 Val Ser Phe Ser Ala Asp Arg Ala Asn ValLys Ala Ser Asp Leu Ile 15 20 25 30 gcc cag gcc acc aga gac ggc tgt ccacca cat ccc gtt cct ggc atg 144 Ala Gln Ala Thr Arg Asp Gly Cys Pro ProHis Pro Val Pro Gly Met 35 40 45 cat aag tgc atg tgt act aat aca tgt ggttgaagacgct gatgctccag 194 His Lys Cys Met Cys Thr Asn Thr Cys Gly 50 55gaccctctga accacgacct cgag 218 374 56 PRT Conus tessulatus 374 Met PheThr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val Val Ser 1 5 10 15 PheSer Ala Asp Arg Ala Asn Val Lys Ala Ser Asp Leu Ile Ala Gln 20 25 30 AlaThr Arg Asp Gly Cys Pro Pro His Pro Val Pro Gly Met His Lys 35 40 45 CysMet Cys Thr Asn Thr Cys Gly 50 55 375 20 PRT Conus tessulatus PEPTIDE(1)..(20) Xaa at residues 4, 5, 7 and 9 is Pro or hydroxy-Pro 375 AspGly Cys Xaa Xaa His Xaa Val Xaa Gly Met His Lys Cys Met Cys 1 5 10 15Thr Asn Thr Cys 20 376 536 DNA Conus geographus CDS (400)..(510)misc_feature (1)..(536) n is unknown 376 anntagantn tgtcgtantannggatcnta antantgnnt cganatgatn angagtgata 60 aatgannggt gcactnntanttangntnnt angatnnnna tattatnnta nnnnntaana 120 natatnggtn nggannaagaagantaaaag tanngnttng tgaaanaang annnnatgtt 180 nnanntcata acnnnaatgtaaataatana cgnnccagtg tgaaannntn tcnnnnataa 240 aaattctntn tntnaangtnnntgtntgng tgtgtgtgtg tgtgtgtgtg tgtgngtgtg 300 tgngtgtgtg tgtgtgtgtgtgtgtgtgtg ngtgtgtgtn tgtgngtgtg tgtgtgtgtg 360 tgtgtgtgtg tgtgtgtgtntgtggttctg ggtccagca tct gat gnc agg gat 414 Ser Asp Xaa Arg Asp 1 5 gacaca gcc aaa gac gaa ggg tct nac atg gac aaa ttg gtc gag aaa 462 Asp ThrAla Lys Asp Glu Gly Ser Xaa Met Asp Lys Leu Val Glu Lys 10 15 20 aaa gaatgt tgc cat cct gcc tgt ggc aaa cac tac agt tgt gga cgc 510 Lys Glu CysCys His Pro Ala Cys Gly Lys His Tyr Ser Cys Gly Arg 25 30 35 tgatgctccagggtntgaag gancaa 536 377 37 PRT Conus geographus misc_feature (1)..(37)Xaa is unknown 377 Ser Asp Xaa Arg Asp Asp Thr Ala Lys Asp Glu Gly SerXaa Met Asp 1 5 10 15 Lys Leu Val Glu Lys Lys Glu Cys Cys His Pro AlaCys Gly Lys His 20 25 30 Tyr Ser Cys Gly Arg 35 378 13 PRT Conusgeographus PEPTIDE (1)..(13) Xaa at residue 1 is Glu orgamma-carboxy-Glu; Xaa at residue 5 is Pro or hydroxy-Pro; Xaa atresidue 11 is Tyr, 125I-Tyr, mono- iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyror O-phospho-Tyr 378 Xaa Cys Cys His Xaa Ala Cys Gly Lys His Xaa Ser Cys1 5 10 379 217 DNA Conus geographus CDS (7)..(183) 379 ggatcc atg ttcacc gtg ttt ctg ttg gtg gtc ttg gca acc act gtc 48 Met Phe Thr Val PheLeu Leu Val Val Leu Ala Thr Thr Val 1 5 10 gtt tcc ttc cct tca gaa cgtgca tct gat ggc agg gat gac aca gcc 96 Val Ser Phe Pro Ser Glu Arg AlaSer Asp Gly Arg Asp Asp Thr Ala 15 20 25 30 aaa gac gaa ggg tct gac atggag aaa ttg gtc gag aaa aaa gaa tgt 144 Lys Asp Glu Gly Ser Asp Met GluLys Leu Val Glu Lys Lys Glu Cys 35 40 45 tgc aat cct gcc tgt ggc aga cacttc agt tgt gga cgc tgatgctcca 193 Cys Asn Pro Ala Cys Gly Arg His PheSer Cys Gly Arg 50 55 ggaccctctg aaccacgact cgag 217 380 59 PRT Conusgeographus 380 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr ValVal Ser 1 5 10 15 Phe Pro Ser Glu Arg Ala Ser Asp Gly Arg Asp Asp ThrAla Lys Asp 20 25 30 Glu Gly Ser Asp Met Glu Lys Leu Val Glu Lys Lys GluCys Cys Asn 35 40 45 Pro Ala Cys Gly Arg His Phe Ser Cys Gly Arg 50 55381 13 PRT Conus geographus PEPTIDE (1)..(13) Xaa at residue 1 is Glu orgamma-carboxy-Glu; Xaa at residue 5 is Pro or hydroxy-Pro 381 Xaa CysCys Asn Xaa Ala Cys Gly Arg His Phe Ser Cys 1 5 10 382 224 DNA Conusstriatus CDS (7)..(207) 382 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtcttg gca acc act gtc 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala ThrThr Val 1 5 10 gtt tcc ttc act tca gat cgt gca tct gat ggc agg gat gacgaa gcc 96 Val Ser Phe Thr Ser Asp Arg Ala Ser Asp Gly Arg Asp Asp GluAla 15 20 25 30 aaa gac gaa agg tct gac atg cac gaa tcg gac cgg aaa ggacgc gca 144 Lys Asp Glu Arg Ser Asp Met His Glu Ser Asp Arg Lys Gly ArgAla 35 40 45 tac tgt tgc cat cct gcc tgt ggc cca aac tat agt tgt ggc acctca 192 Tyr Cys Cys His Pro Ala Cys Gly Pro Asn Tyr Ser Cys Gly Thr Ser50 55 60 tgc tcc agg acc ctc tgaaccacga cctcgag 224 Cys Ser Arg Thr Leu65 383 67 PRT Conus striatus 383 Met Phe Thr Val Phe Leu Leu Val Val LeuAla Thr Thr Val Val Ser 1 5 10 15 Phe Thr Ser Asp Arg Ala Ser Asp GlyArg Asp Asp Glu Ala Lys Asp 20 25 30 Glu Arg Ser Asp Met His Glu Ser AspArg Lys Gly Arg Ala Tyr Cys 35 40 45 Cys His Pro Ala Cys Gly Pro Asn TyrSer Cys Gly Thr Ser Cys Ser 50 55 60 Arg Thr Leu 65 384 22 PRT Conusstriatus PEPTIDE (1)..(22) Xaa at residues 6 and 10 is Pro orhydroxy-Pro; Xaa at residues 2 and 12 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho- Tyr or O-phospho-Tyr 384 Ala Xaa Cys Cys His XaaAla Cys Gly Xaa Asn Xaa Ser Cys Gly Thr 1 5 10 15 Ser Cys Ser Arg ThrLeu 20 385 224 DNA Conus striatus CDS (7)..(189) 385 ggatcc atg ttc accgtg ttt ctg ttg gtt gtc ttg gca acc act gtc 48 Met Phe Thr Val Phe LeuLeu Val Val Leu Ala Thr Thr Val 1 5 10 gtt tcc ttc act tca gat cgt gcatct gat ggc agg gat gac gaa gcc 96 Val Ser Phe Thr Ser Asp Arg Ala SerAsp Gly Arg Asp Asp Glu Ala 15 20 25 30 aaa gac gaa agg tct gac atg cacgaa tcg gac cgg aaa gga cgc gca 144 Lys Asp Glu Arg Ser Asp Met His GluSer Asp Arg Lys Gly Arg Ala 35 40 45 tac tgt tgc cat cct gtc tgt ggc aaaaac ttt gat tgt gga cgc 189 Tyr Cys Cys His Pro Val Cys Gly Lys Asn PheAsp Cys Gly Arg 50 55 60 tgatgctcca ggaccctctg aaccacgacc tcgag 224 38661 PRT Conus striatus 386 Met Phe Thr Val Phe Leu Leu Val Val Leu AlaThr Thr Val Val Ser 1 5 10 15 Phe Thr Ser Asp Arg Ala Ser Asp Gly ArgAsp Asp Glu Ala Lys Asp 20 25 30 Glu Arg Ser Asp Met His Glu Ser Asp ArgLys Gly Arg Ala Tyr Cys 35 40 45 Cys His Pro Val Cys Gly Lys Asn Phe AspCys Gly Arg 50 55 60 387 14 PRT Conus striatus PEPTIDE (1)..(14) Xaa atresidue 6 is Pro or hydroxy-Pro; Xaa at residue 2 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 387 Ala XaaCys Cys His Xaa Val Cys Gly Lys Asn Phe Asp Cys 1 5 10 388 224 DNA Conusrattus CDS (7)..(207) 388 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtc ttggca acc act gtc 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr ThrVal 1 5 10 gtt tcc ttc cct tca gat cgt gca tct gat ggc agg gat gac gaagcc 96 Val Ser Phe Pro Ser Asp Arg Ala Ser Asp Gly Arg Asp Asp Glu Ala15 20 25 30 aaa gac gaa agg tct gac atg cac gaa tcg gac cgg aat gga cgcgga 144 Lys Asp Glu Arg Ser Asp Met His Glu Ser Asp Arg Asn Gly Arg Gly35 40 45 tgc tgt tgc aat cct gcc tgt ggc cca aac tat ggt tgt ggc acc tca192 Cys Cys Cys Asn Pro Ala Cys Gly Pro Asn Tyr Gly Cys Gly Thr Ser 5055 60 tgc tcc agg acc ctc tgaaccacga cctcgag 224 Cys Ser Arg Thr Leu 65389 67 PRT Conus rattus 389 Met Phe Thr Val Phe Leu Leu Val Val Leu AlaThr Thr Val Val Ser 1 5 10 15 Phe Pro Ser Asp Arg Ala Ser Asp Gly ArgAsp Asp Glu Ala Lys Asp 20 25 30 Glu Arg Ser Asp Met His Glu Ser Asp ArgAsn Gly Arg Gly Cys Cys 35 40 45 Cys Asn Pro Ala Cys Gly Pro Asn Tyr GlyCys Gly Thr Ser Cys Ser 50 55 60 Arg Thr Leu 65 390 22 PRT Conus rattusPEPTIDE (1)..(22) Xaa at residues 6 and 10 is Pro or hydroxy-Pro; Xaa atresidue 12 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 390 Gly Cys Cys Cys Asn Xaa Ala Cys Gly Xaa Asn Xaa GlyCys Gly Thr 1 5 10 15 Ser Cys Ser Arg Thr Leu 20 391 230 DNA Conusarenatus CDS (7)..(195) 391 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtcttg gca acc act gtg 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala ThrThr Val 1 5 10 gat tcc ttc act cca gtt cgt act tct gtt ggc agg agt gctgca gcc 96 Asp Ser Phe Thr Pro Val Arg Thr Ser Val Gly Arg Ser Ala AlaAla 15 20 25 30 aac gcg ttt gac cgg atc gct ctg acc gcc agg caa gat tattgc tgt 144 Asn Ala Phe Asp Arg Ile Ala Leu Thr Ala Arg Gln Asp Tyr CysCys 35 40 45 acc att ccc agc tgt tgg gat cgc tat aaa gag aga tgt aga cacata 192 Thr Ile Pro Ser Cys Trp Asp Arg Tyr Lys Glu Arg Cys Arg His Ile50 55 60 cgc tgatgctcca ggaccctctg aaccacgacc ttgag 230 Arg 392 63 PRTConus arenatus 392 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr ThrVal Asp Ser 1 5 10 15 Phe Thr Pro Val Arg Thr Ser Val Gly Arg Ser AlaAla Ala Asn Ala 20 25 30 Phe Asp Arg Ile Ala Leu Thr Ala Arg Gln Asp TyrCys Cys Thr Ile 35 40 45 Pro Ser Cys Trp Asp Arg Tyr Lys Glu Arg Cys ArgHis Ile Arg 50 55 60 393 22 PRT Conus arenatus PEPTIDE (1)..(22) Xaa atresidue 1 is Gln or pyro-Gln; Xaa at residue 16 is Glu orgamma-carboxy-Glu; Xaa at residue 8 is Pro or hydroxy-Pro; Xaa atresidue 11 is Trp (D or L) or bromo-Trp (D or L); 393 Xaa Asp Xaa CysCys Thr Ile Xaa Ser Cys Xaa Asp Arg Xaa Lys Xaa 1 5 10 15 Arg Cys ArgHis Ile Arg 20 394 230 DNA Conus eburneus CDS (7)..(195) 394 ggatcc atgttc acc gtg ttt ctg ttg gtt gtc ttg gca acc act gtg 48 Met Phe Thr ValPhe Leu Leu Val Val Leu Ala Thr Thr Val 1 5 10 gat tcc ttc act tca gttcgt act tcc gtt ggc agg agt gct gca gcc 96 Asp Ser Phe Thr Ser Val ArgThr Ser Val Gly Arg Ser Ala Ala Ala 15 20 25 30 aac gcg ttt gac cgg atcgct ctg acc gcc agg caa gat tat tgc tgt 144 Asn Ala Phe Asp Arg Ile AlaLeu Thr Ala Arg Gln Asp Tyr Cys Cys 35 40 45 acc att ccc agc tgt tgg gatcgc tat aaa gag aga tgt aga cac ata 192 Thr Ile Pro Ser Cys Trp Asp ArgTyr Lys Glu Arg Cys Arg His Ile 50 55 60 cgc tgatgctcca ggaccctctgaaccacgacc tcgag 230 Arg 395 63 PRT Conus eburneus 395 Met Phe Thr ValPhe Leu Leu Val Val Leu Ala Thr Thr Val Asp Ser 1 5 10 15 Phe Thr SerVal Arg Thr Ser Val Gly Arg Ser Ala Ala Ala Asn Ala 20 25 30 Phe Asp ArgIle Ala Leu Thr Ala Arg Gln Asp Tyr Cys Cys Thr Ile 35 40 45 Pro Ser CysTrp Asp Arg Tyr Lys Glu Arg Cys Arg His Ile Arg 50 55 60 396 22 PRTConus eburneus PEPTIDE (1)..(22) Xaa at residue 1 is Gln or pyro-Gln;Xaa at residue 16 is Glu or gamma-carboxy-Glu; Xaa at residue 8 is Proor hydroxy-Pro; Xaa at residue 11 is Trp (D or L) or bromo-Trp (D or L);396 Xaa Asp Xaa Cys Cys Thr Ile Xaa Ser Cys Xaa Asp Arg Xaa Lys Xaa 1 510 15 Arg Cys Arg His Ile Arg 20 397 221 DNA Conus miles CDS (7)..(177)397 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtc ttg gca act gct gtt 48Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Ala Val 1 5 10 ctt ccagtc act tta gat cgt gca tct gat gga agg aat gca gca gcc 96 Leu Pro ValThr Leu Asp Arg Ala Ser Asp Gly Arg Asn Ala Ala Ala 15 20 25 30 aac gccaaa acg cct cgc ctg atc gcg cca ttc atc agg gat tat tgc 144 Asn Ala LysThr Pro Arg Leu Ile Ala Pro Phe Ile Arg Asp Tyr Cys 35 40 45 tgt cat agaggt ccc tgt atg gta tgg tgt ggt tgaagccgct gctgctccag 197 Cys His ArgGly Pro Cys Met Val Trp Cys Gly 50 55 gaccctctga accacgacct cgag 221 39857 PRT Conus miles 398 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala ThrAla Val Leu Pro 1 5 10 15 Val Thr Leu Asp Arg Ala Ser Asp Gly Arg AsnAla Ala Ala Asn Ala 20 25 30 Lys Thr Pro Arg Leu Ile Ala Pro Phe Ile ArgAsp Tyr Cys Cys His 35 40 45 Arg Gly Pro Cys Met Val Trp Cys Gly 50 55399 13 PRT Conus miles PEPTIDE (1)..(13) Xaa at residue 8 is Pro orhydroxy-Pro; Xaa at residue 12 is Trp (D or L) or bromo-Trp (D or L);Xaa at residue 2 is Tyr, 125I-Tyr , mono-iodo-Tyr, di-iodo-Tyr,O-sulpho-Tyr or O-phospho-Tyr 399 Asp Xaa Cys Cys His Arg Gly Xaa CysMet Val Xaa Cys 1 5 10 400 218 DNA Conus jDedius CDS (7)..(174) 400ggatcc atg ttc acc gtg ttt ctg ttg gtt gtc ttg gca acc act gtc 48 MetPhe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val 1 5 10 gtt tcc aactct tca gat cgt ggt cca gca tct aat aaa agg aag aat 96 Val Ser Asn SerSer Asp Arg Gly Pro Ala Ser Asn Lys Arg Lys Asn 15 20 25 30 gcc gca atgctt gac atg atc gct caa cac gcc ata agg ggt tgc tgt 144 Ala Ala Met LeuAsp Met Ile Ala Gln His Ala Ile Arg Gly Cys Cys 35 40 45 tcc gat cct cgctgt aga tat aga tgt cgt tgaagacgct gctgctccag 194 Ser Asp Pro Arg CysArg Tyr Arg Cys Arg 50 55 gaccctctga accacgacct cgag 218 401 56 PRTConus jDedius 401 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr ThrVal Val Ser 1 5 10 15 Asn Ser Ser Asp Arg Gly Pro Ala Ser Asn Lys ArgLys Asn Ala Ala 20 25 30 Met Leu Asp Met Ile Ala Gln His Ala Ile Arg GlyCys Cys Ser Asp 35 40 45 Pro Arg Cys Arg Tyr Arg Cys Arg 50 55 402 13PRT Conus jDedius PEPTIDE (1)..(13) Xaa at residue 6 is Pro orhydroxy-Pro;Xaa at residue 10 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 402 Gly Cys Cys Ser Asp XaaArg Cys Arg Xaa Arg Cys Arg 1 5 10 403 17 PRT Conus omaria PEPTIDE(1)..(17) Xaa at residues 6 and 13 is Pro or hydroxy-Pro 403 Gly Cys CysSer His Xaa Ala Cys Asn Val Asn Asn Xaa His Ile Cys 1 5 10 15 Gly 404 17PRT Conus omaria PEPTIDE (1)..(17) Xaa at residues 6 and 13 is Pro orhydroxy-Pro; C terminus contai ns free hydroxyl 404 Gly Cys Cys Ser HisXaa Ala Cys Asn Val Asn Asn Xaa His Ile Cys 1 5 10 15 Gly 405 224 DNAConus quercinus CDS (7)..(189) 405 ggatcc atg ttc acc gtg ttt ctg ttggtt gtc ttg gca acc act gtc 48 Met Phe Thr Val Phe Leu Leu Val Val LeuAla Thr Thr Val 1 5 10 act tca gat cgt gta tct aat ggc agg aaa gct gcagcc aaa ttc aaa 96 Thr Ser Asp Arg Val Ser Asn Gly Arg Lys Ala Ala AlaLys Phe Lys 15 20 25 30 gcg cct gcc ctg atg gag ctg tcc gtc agg caa ggatgc tgt tca gat 144 Ala Pro Ala Leu Met Glu Leu Ser Val Arg Gln Gly CysCys Ser Asp 35 40 45 cct gcc tgt gcc gtg agc aat cca gac atc tgt ggc ggagga cgc 189 Pro Ala Cys Ala Val Ser Asn Pro Asp Ile Cys Gly Gly Gly Arg50 55 60 tgatgctcca ggaccctctg aaccacgacc tcgag 224 406 61 PRT Conusquercinus 406 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr ValThr Ser 1 5 10 15 Asp Arg Val Ser Asn Gly Arg Lys Ala Ala Ala Lys PheLys Ala Pro 20 25 30 Ala Leu Met Glu Leu Ser Val Arg Gln Gly Cys Cys SerAsp Pro Ala 35 40 45 Cys Ala Val Ser Asn Pro Asp Ile Cys Gly Gly Gly Arg50 55 60 407 19 PRT Conus quercinus PEPTIDE (1)..(19) Xaa at residue 1is Gln or pyro-Gln; Xaa at residues 7 and 14 is Pro or hydroxy-Pro 407Xaa Gly Cys Cys Ser Asp Xaa Ala Cys Ala Val Ser Asn Xaa Asp Ile 1 5 1015 Cys Gly Gly 408 230 DNA Conus bandanus CDS (7)..(186) 408 ggatcc atgttc acc gtg ttt ctg ttg gtt gtc ttg gca acc act gtt 48 Met Phe Thr ValPhe Leu Leu Val Val Leu Ala Thr Thr Val 1 5 10 gtt tcc ttc act tca aatcgt gca ttt cgt cgt agg aat gcc gta gcc 96 Val Ser Phe Thr Ser Asn ArgAla Phe Arg Arg Arg Asn Ala Val Ala 15 20 25 30 aaa gcg tct gac ctg atcgct ctg aac gcc agg aga cca gaa tgc tgt 144 Lys Ala Ser Asp Leu Ile AlaLeu Asn Ala Arg Arg Pro Glu Cys Cys 35 40 45 act cat cct gcc tgt cac gtgagt cat cca gaa ctc tgt ggt 186 Thr His Pro Ala Cys His Val Ser His ProGlu Leu Cys Gly 50 55 60 tgaagacgct gacgctccag gaccctctga accacgacctcgag 230 409 60 PRT Conus bandanus 409 Met Phe Thr Val Phe Leu Leu ValVal Leu Ala Thr Thr Val Val Ser 1 5 10 15 Phe Thr Ser Asn Arg Ala PheArg Arg Arg Asn Ala Val Ala Lys Ala 20 25 30 Ser Asp Leu Ile Ala Leu AsnAla Arg Arg Pro Glu Cys Cys Thr His 35 40 45 Pro Ala Cys His Val Ser HisPro Glu Leu Cys Gly 50 55 60 410 17 PRT Conus bandanus PEPTIDE (1)..(17)Xaa at residues 2 and 15 is Glu or gamma-carboxy-Glu; Xaa at residues 1,7 and 14 is Pro or hydroxy-Pro 410 Xaa Xaa Cys Cys Thr His Xaa Ala CysHis Val Ser His Xaa Xaa Leu 1 5 10 15 Cys 411 242 DNA Conus marmoreusCDS (7)..(198) 411 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtc ttg gcaacc act gtt 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val 15 10 gtt tcc ttc act tca aat cgt gtt ctg gat cca gca ttt cgt cgt agg 96Val Ser Phe Thr Ser Asn Arg Val Leu Asp Pro Ala Phe Arg Arg Arg 15 20 2530 aat gcc gca gcc aaa gcg tct gac ctg atc gct ctg aac gcc agg aga 144Asn Ala Ala Ala Lys Ala Ser Asp Leu Ile Ala Leu Asn Ala Arg Arg 35 40 45cca gaa tgc tgt act cat cct gcc tgt cac gtg agt aat cca gaa ctc 192 ProGlu Cys Cys Thr His Pro Ala Cys His Val Ser Asn Pro Glu Leu 50 55 60 tgtggc tgaagacgct gatgctccag gaccctctga accacgacct cgag 242 Cys Gly 412 64PRT Conus marmoreus 412 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala ThrThr Val Val Ser 1 5 10 15 Phe Thr Ser Asn Arg Val Leu Asp Pro Ala PheArg Arg Arg Asn Ala 20 25 30 Ala Ala Lys Ala Ser Asp Leu Ile Ala Leu AsnAla Arg Arg Pro Glu 35 40 45 Cys Cys Thr His Pro Ala Cys His Val Ser AsnPro Glu Leu Cys Gly 50 55 60 413 17 PRT Conus marmoreus PEPTIDE(1)..(17) Xaa at residues 2 and 15 is Glu or gamma-carboxy-Glu; Xaa atresidues 1, 7 and 14 is Pro or hydroxy-Pro 413 Xaa Xaa Cys Cys Thr HisXaa Ala Cys His Val Ser Asn Xaa Xaa Leu 1 5 10 15 Cys 414 218 DNA Conusmiles CDS (7)..(174) 414 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtc ttggca acc act gtc 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr ThrVal 1 5 10 gtt tcc gtc act tca tat cgt gca tct cat ggc agg aag gac gcagcc 96 Val Ser Val Thr Ser Tyr Arg Ala Ser His Gly Arg Lys Asp Ala Ala15 20 25 30 gac ctg agc gct ctg aac gac aac aat aat tgc tgt aac cat cctgcc 144 Asp Leu Ser Ala Leu Asn Asp Asn Asn Asn Cys Cys Asn His Pro Ala35 40 45 tgt gcc ggg aaa aat tca gat ctt tgt ggt tgaagacgct gctgctccag194 Cys Ala Gly Lys Asn Ser Asp Leu Cys Gly 50 55 gaccctctga accacgacctcgag 218 415 56 PRT Conus miles 415 Met Phe Thr Val Phe Leu Leu Val ValLeu Ala Thr Thr Val Val Ser 1 5 10 15 Val Thr Ser Tyr Arg Ala Ser HisGly Arg Lys Asp Ala Ala Asp Leu 20 25 30 Ser Ala Leu Asn Asp Asn Asn AsnCys Cys Asn His Pro Ala Cys Ala 35 40 45 Gly Lys Asn Ser Asp Leu Cys Gly50 55 416 15 PRT Conus miles PEPTIDE (1)..(15) Xaa at residue 5 is Proor hydroxy-Pro 416 Cys Cys Asn His Xaa Ala Cys Ala Gly Lys Asn Ser AspLeu Cys 1 5 10 15 417 16 PRT Conus magus PEPTIDE (1)..(16) Xaa atresidue 11 is Glu or gamma-carboxy-Glu; Xaa at residue 6 is Pro orhydroxy-Pro; Xaa at residue 4 is Tyr, 125I-Tyr, mono- iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 417 Gly Cys Cys Xaa His XaaThr Cys His Leu Xaa His Ser Asn Leu Cys 1 5 10 15 418 224 DNA Conusnobilis CDS (7)..(189) misc_feature (1)..(224) n is unknown 418 ggatccatg ttc acc gtg ttt ctg ttg gtt gtc ttg gca acc act gtt 48 Met Phe ThrVal Phe Leu Leu Val Val Leu Ala Thr Thr Val 1 5 10 gtt tcc ttc act tcagat cgt gca tct gat ggc agg aat gcc gca gcc 96 Val Ser Phe Thr Ser AspArg Ala Ser Asp Gly Arg Asn Ala Ala Ala 15 20 25 30 aaa gct tct gac ctgatt gct ttg acc gtc agg gga tgc tgt gag cga 144 Lys Ala Ser Asp Leu IleAla Leu Thr Val Arg Gly Cys Cys Glu Arg 35 40 45 cct ccc tgt cgc tgg caaaat cca gat ctt tgt ggt gga agg cgc 189 Pro Pro Cys Arg Trp Gln Asn ProAsp Leu Cys Gly Gly Arg Arg 50 55 60 tganattcca ggaccctctg aaccacgacctcgag 224 419 61 PRT Conus nobilis 419 Met Phe Thr Val Phe Leu Leu ValVal Leu Ala Thr Thr Val Val Ser 1 5 10 15 Phe Thr Ser Asp Arg Ala SerAsp Gly Arg Asn Ala Ala Ala Lys Ala 20 25 30 Ser Asp Leu Ile Ala Leu ThrVal Arg Gly Cys Cys Glu Arg Pro Pro 35 40 45 Cys Arg Trp Gln Asn Pro AspLeu Cys Gly Gly Arg Arg 50 55 60 420 17 PRT Conus nobilis PEPTIDE(1)..(17) Xaa at residue 4 is Glu or gamma-carboxy-Glu; Xaa at residues6, 7 and 13 is Pro or hydroxy-Pro; Xaa at residue 10 is Trp (D or L) orbromo-Trp (D or L) 420 Gly Cys Cys Xaa Arg Xaa Xaa Cys Arg Xaa Gln AsnXaa Asp Leu Cys 1 5 10 15 Gly 421 233 DNA Conus atlanticus CDS(7)..(198) 421 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtc ttg gca accaca gtc 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val 1 510 gtt tcc ttc act tca gat agt gca ttt gat agc agg aat gtc gca gcc 96Val Ser Phe Thr Ser Asp Ser Ala Phe Asp Ser Arg Asn Val Ala Ala 15 20 2530 aac gac aaa gtg tct gac atg atc gct ctg acc gcc agg aga aca tgc 144Asn Asp Lys Val Ser Asp Met Ile Ala Leu Thr Ala Arg Arg Thr Cys 35 40 45tgt tcc cgt cct acc tgt aga atg gaa tat cca gaa ctt tgt ggt gga 192 CysSer Arg Pro Thr Cys Arg Met Glu Tyr Pro Glu Leu Cys Gly Gly 50 55 60 agacgc tgatactcca ggaccctctg aaccacgacc tcgag 233 Arg Arg 422 64 PRT Conusatlanticus 422 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr ValVal Ser 1 5 10 15 Phe Thr Ser Asp Ser Ala Phe Asp Ser Arg Asn Val AlaAla Asn Asp 20 25 30 Lys Val Ser Asp Met Ile Ala Leu Thr Ala Arg Arg ThrCys Cys Ser 35 40 45 Arg Pro Thr Cys Arg Met Glu Tyr Pro Glu Leu Cys GlyGly Arg Arg 50 55 60 423 17 PRT Conus atlanticus PEPTIDE (1)..(17) Xaaat residues 11 and 14 is Glu or gamma-carboxy-Glu; Xaa at residues 6 and13 is Pro or hydroxy-Pro; Xaa at residue 12 is Tyr, 125I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 423 Thr CysCys Ser Arg Xaa Thr Cys Arg Met Xaa Xaa Xaa Xaa Leu Cys 1 5 10 15 Gly424 227 DNA Conus quercinus CDS (7)..(192) misc_feature (1)..(227) n isunknown 424 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtc ttg gca atc acggtg 48 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Ile Thr Val 1 5 10gtt tcc ttc acc tca gat cat gca tct gat ggc agg aat acc gca gcc 96 ValSer Phe Thr Ser Asp His Ala Ser Asp Gly Arg Asn Thr Ala Ala 15 20 25 30aac gac aaa gcg tct aaa ctg atg gct ctt acg aac gaa tgc tgt gac 144 AsnAsp Lys Ala Ser Lys Leu Met Ala Leu Thr Asn Glu Cys Cys Asp 35 40 45 aatcct ccg tgc aag tcg agt aat cca gat ttg tgt gac tgg aga agc 192 Asn ProPro Cys Lys Ser Ser Asn Pro Asp Leu Cys Asp Trp Arg Ser 50 55 60tgatgctcca ggaccctntg aaccacgacc tcgag 227 425 62 PRT Conus quercinus425 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Ile Thr Val Val Ser 1 510 15 Phe Thr Ser Asp His Ala Ser Asp Gly Arg Asn Thr Ala Ala Asn Asp 2025 30 Lys Ala Ser Lys Leu Met Ala Leu Thr Asn Glu Cys Cys Asp Asn Pro 3540 45 Pro Cys Lys Ser Ser Asn Pro Asp Leu Cys Asp Trp Arg Ser 50 55 60426 21 PRT Conus quercinus PEPTIDE (1)..(21) Xaa at residue 2 is Glu orgamma-carboxy-Glu; Xaa at residues 7, 8 and 14 is Pro or hydroxy-Pro;Xaa at residue 19 is Trp (D or L) or bromo-Trp (D or L) 426 Asn Xaa CysCys Asp Asn Xaa Xaa Cys Lys Ser Ser Asn Xaa Asp Leu 1 5 10 15 Cys AspXaa Arg Ser 20 427 221 DNA Conus leopardus CDS (7)..(177) 427 ggatcc atgttc acc gtg ttt ctg ttg gtt gtc ttg gca acc acg gtc 48 Met Phe Thr ValPhe Leu Leu Val Val Leu Ala Thr Thr Val 1 5 10 gtt tcc ctc act tta gatcgt gca tct ggt ggc agg aga tct gga gcc 96 Val Ser Leu Thr Leu Asp ArgAla Ser Gly Gly Arg Arg Ser Gly Ala 15 20 25 30 gac aac atg att gct cttctg atc atc aga aaa tgc tgt tcc aat ccc 144 Asp Asn Met Ile Ala Leu LeuIle Ile Arg Lys Cys Cys Ser Asn Pro 35 40 45 gcc tgt aac agg tat aat ccagca att tgt gat tgaagacgct aatgctccag 197 Ala Cys Asn Arg Tyr Asn ProAla Ile Cys Asp 50 55 gaccctctga accacgacct cgag 221 428 57 PRT Conusleopardus 428 Met Phe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr ValVal Ser 1 5 10 15 Leu Thr Leu Asp Arg Ala Ser Gly Gly Arg Arg Ser GlyAla Asp Asn 20 25 30 Met Ile Ala Leu Leu Ile Ile Arg Lys Cys Cys Ser AsnPro Ala Cys 35 40 45 Asn Arg Tyr Asn Pro Ala Ile Cys Asp 50 55 429 16PRT Conus leopardus PEPTIDE (1)..(16) Xaa at residues 5 and 12 is Pro orhydroxy-Pro; Xaa at residue 10 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 429 Cys Cys Ser Asn Xaa AlaCys Asn Arg Xaa Asn Xaa Ala Ile Cys Asp 1 5 10 15 430 224 DNA Conusemaciatus CDS (7)..(180) 430 ggatcc atg ttc acc gtg ttt ctg ttg gtt ctcttg gca acc act gtc 48 Met Phe Thr Val Phe Leu Leu Val Leu Leu Ala ThrThr Val 1 5 10 act tta cat cgt gca tct aat ggc agg aat gcc gca gcc agcagg aaa 96 Thr Leu His Arg Ala Ser Asn Gly Arg Asn Ala Ala Ala Ser ArgLys 15 20 25 30 gcg tct gcc ctg atc gct cag atc gcc ggt aga gac tgc tgtaac ttt 144 Ala Ser Ala Leu Ile Ala Gln Ile Ala Gly Arg Asp Cys Cys AsnPhe 35 40 45 cct gct tgt gcc gcg agt aat cca ggc ctt tgt act tgaagacgct190 Pro Ala Cys Ala Ala Ser Asn Pro Gly Leu Cys Thr 50 55 gctgctccaggaccctctga accacgacct cgag 224 431 58 PRT Conus emaciatus 431 Met PheThr Val Phe Leu Leu Val Leu Leu Ala Thr Thr Val Thr Leu 1 5 10 15 HisArg Ala Ser Asn Gly Arg Asn Ala Ala Ala Ser Arg Lys Ala Ser 20 25 30 AlaLeu Ile Ala Gln Ile Ala Gly Arg Asp Cys Cys Asn Phe Pro Ala 35 40 45 CysAla Ala Ser Asn Pro Gly Leu Cys Thr 50 55 432 17 PRT Conus emaciatusPEPTIDE (1)..(17) Xaa at residues 6 and 13 is Pro or hydroxy-Pro 432 AspCys Cys Asn Phe Xaa Ala Cys Ala Ala Ser Asn Xaa Gly Leu Cys 1 5 10 15Thr 433 215 DNA Conus victor CDS (7)..(180) 433 ggatcc atg ttc acc gtgttt ctg ttg gtt gtc ttg gca acc acc atc 48 Met Phe Thr Val Phe Leu LeuVal Val Leu Ala Thr Thr Ile 1 5 10 gtt tcc tcc act tta gat cgt gca tctgat ggc atg aat gct gca gcg 96 Val Ser Ser Thr Leu Asp Arg Ala Ser AspGly Met Asn Ala Ala Ala 15 20 25 30 tct gac ctg atc gct ctg agc atc aggaga tgc tgt tct tct cct ccc 144 Ser Asp Leu Ile Ala Leu Ser Ile Arg ArgCys Cys Ser Ser Pro Pro 35 40 45 tgt ttc gcg agt aat cca gct tgt ggt agacga cgc tgatgctcca 190 Cys Phe Ala Ser Asn Pro Ala Cys Gly Arg Arg Arg50 55 ggaccctctg aaccacgacc tcgag 215 434 58 PRT Conus victor 434 MetPhe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Ile Val Ser 1 5 10 15Ser Thr Leu Asp Arg Ala Ser Asp Gly Met Asn Ala Ala Ala Ser Asp 20 25 30Leu Ile Ala Leu Ser Ile Arg Arg Cys Cys Ser Ser Pro Pro Cys Phe 35 40 45Ala Ser Asn Pro Ala Cys Gly Arg Arg Arg 50 55 435 14 PRT Conus victorPEPTIDE (1)..(14) Xaa at residues 5, 6 and 13 is Pro or hydroxy-Pro 435Cys Cys Ser Ser Xaa Xaa Cys Phe Ala Ser Asn Xaa Ala Cys 1 5 10 436 230DNA Conus cinereus gubba CDS (7)..(195) 436 ggatcc atg ttc acc gtg tttctg ttg gtt gtc ctg gca acc act atc 48 Met Phe Thr Val Phe Leu Leu ValVal Leu Ala Thr Thr Ile 1 5 10 gtt tcc tcc act tca ggt cat gca ttt gatggc agg aat gct gca gcc 96 Val Ser Ser Thr Ser Gly His Ala Phe Asp GlyArg Asn Ala Ala Ala 15 20 25 30 gac tac aaa ggg tct gaa ttg ctt gct atgacc gtc agg gga gga tgc 144 Asp Tyr Lys Gly Ser Glu Leu Leu Ala Met ThrVal Arg Gly Gly Cys 35 40 45 tgt tcc ttt cct ccc tgt atc gca aat aat cctttt tgt gct gga aga 192 Cys Ser Phe Pro Pro Cys Ile Ala Asn Asn Pro PheCys Ala Gly Arg 50 55 60 cgc tgatgctcca ggaccctctg aaccacgacc tcgag 230Arg 437 63 PRT Conus cinereus gubba 437 Met Phe Thr Val Phe Leu Leu ValVal Leu Ala Thr Thr Ile Val Ser 1 5 10 15 Ser Thr Ser Gly His Ala PheAsp Gly Arg Asn Ala Ala Ala Asp Tyr 20 25 30 Lys Gly Ser Glu Leu Leu AlaMet Thr Val Arg Gly Gly Cys Cys Ser 35 40 45 Phe Pro Pro Cys Ile Ala AsnAsn Pro Phe Cys Ala Gly Arg Arg 50 55 60 438 17 PRT Conus cinereus gubbaPEPTIDE (1)..(17) Xaa at residues 7, 8 and 14 is Pro or hydroxy-Pro 438Gly Gly Cys Cys Ser Phe Xaa Xaa Cys Ile Ala Asn Asn Xaa Phe Cys 1 5 1015 Ala 439 221 DNA Conus flavidus CDS (7)..(177) 439 ggatcc atg ttc accgtg ttt ctg ttg gtt gtc ttc gca tcc tct gtc 48 Met Phe Thr Val Phe LeuLeu Val Val Phe Ala Ser Ser Val 1 5 10 act tta gat cgt gca tct cat ggcagg tat atc cca gtc gtc gac aga 96 Thr Leu Asp Arg Ala Ser His Gly ArgTyr Ile Pro Val Val Asp Arg 15 20 25 30 gcg tct gcc ctg atg gct cag gccgac ctt aga ggt tgc tgt tcc aat 144 Ala Ser Ala Leu Met Ala Gln Ala AspLeu Arg Gly Cys Cys Ser Asn 35 40 45 cct cct tgt tcc tat ctt aat cca gcctgt ggt taaagacgct gccgctccag 197 Pro Pro Cys Ser Tyr Leu Asn Pro AlaCys Gly 50 55 gaccctctga accacgacct cgag 221 440 57 PRT Conus flavidus440 Met Phe Thr Val Phe Leu Leu Val Val Phe Ala Ser Ser Val Thr Leu 1 510 15 Asp Arg Ala Ser His Gly Arg Tyr Ile Pro Val Val Asp Arg Ala Ser 2025 30 Ala Leu Met Ala Gln Ala Asp Leu Arg Gly Cys Cys Ser Asn Pro Pro 3540 45 Cys Ser Tyr Leu Asn Pro Ala Cys Gly 50 55 441 15 PRT Conusflavidus PEPTIDE (1)..(15) Xaa at residues 6, 7 and 13 is Pro orhydroxy-Pro; Xaa at residue 10 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 441 Gly Cys Cys Ser Asn XaaXaa Cys Ser Xaa Leu Asn Xaa Ala Cys 1 5 10 15 442 221 DNA Conusemaciatus CDS (7)..(177) 442 ggatcc atg ttc acc gtg ttt ctg ttg gtt gtcttc gca tcc tct gtc 48 Met Phe Thr Val Phe Leu Leu Val Val Phe Ala SerSer Val 1 5 10 act tta gat cgt gca tct cat ggc agg tat gcc gca gtc gtcaac aga 96 Thr Leu Asp Arg Ala Ser His Gly Arg Tyr Ala Ala Val Val AsnArg 15 20 25 30 gcg tct gcc ctg atg gct cat gcc gcc ctt cga gat tgc tgttcc gat 144 Ala Ser Ala Leu Met Ala His Ala Ala Leu Arg Asp Cys Cys SerAsp 35 40 45 cct cct tgt gct cat aat aat cca gac tgt cgt taaagacgctgctgctccag 197 Pro Pro Cys Ala His Asn Asn Pro Asp Cys Arg 50 55gaccctctga accacgacct cgag 221 443 57 PRT Conus emaciatus 443 Met PheThr Val Phe Leu Leu Val Val Phe Ala Ser Ser Val Thr Leu 1 5 10 15 AspArg Ala Ser His Gly Arg Tyr Ala Ala Val Val Asn Arg Ala Ser 20 25 30 AlaLeu Met Ala His Ala Ala Leu Arg Asp Cys Cys Ser Asp Pro Pro 35 40 45 CysAla His Asn Asn Pro Asp Cys Arg 50 55 444 16 PRT Conus emaciatus PEPTIDE(1)..(16) Xaa at residues 6, 7 and 13 is Pro or hydroxy-Pro 444 Asp CysCys Ser Asp Xaa Xaa Cys Ala His Asn Asn Xaa Asp Cys Arg 1 5 10 15 445230 DNA Conus generalis CDS (7)..(195) 445 ggatcc atg ttc acc gtg tttctg ttg gtt gtc ttg gca act act gtc 48 Met Phe Thr Val Phe Leu Leu ValVal Leu Ala Thr Thr Val 1 5 10 gtt tcc ttc act tca gat cgt ggg tct gatggc agg aat gcc gca gcc 96 Val Ser Phe Thr Ser Asp Arg Gly Ser Asp GlyArg Asn Ala Ala Ala 15 20 25 30 aag gac aaa gcg tct gac ctg gtc gct ctgacc gtc aag gga tgc tgt 144 Lys Asp Lys Ala Ser Asp Leu Val Ala Leu ThrVal Lys Gly Cys Cys 35 40 45 tct aat cct ccc tgt tac gcg aat aat caa gcctat tgt aat gga aga 192 Ser Asn Pro Pro Cys Tyr Ala Asn Asn Gln Ala TyrCys Asn Gly Arg 50 55 60 cgc tgatgctcca ggaccctctg aaccacgacc tcgag 230Arg 446 63 PRT Conus generalis 446 Met Phe Thr Val Phe Leu Leu Val ValLeu Ala Thr Thr Val Val Ser 1 5 10 15 Phe Thr Ser Asp Arg Gly Ser AspGly Arg Asn Ala Ala Ala Lys Asp 20 25 30 Lys Ala Ser Asp Leu Val Ala LeuThr Val Lys Gly Cys Cys Ser Asn 35 40 45 Pro Pro Cys Tyr Ala Asn Asn GlnAla Tyr Cys Asn Gly Arg Arg 50 55 60 447 17 PRT Conus generalis PEPTIDE(1)..(17) Xaa at residues 6 and 7 is Pro or hydroxy-Pro; Xaa at residues9 and 15 is Tyr, 125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr orO-phospho-Tyr 447 Gly Cys Cys Ser Asn Xaa Xaa Cys Xaa Ala Asn Asn GlnAla Xaa Cys 1 5 10 15 Asn 448 212 DNA Conus wittigi CDS (7)..(195) 448ggatcc atg ttc acc gtg ttt ctg ttg gtt gtc ctg gca acc act gtc 48 MetPhe Thr Val Phe Leu Leu Val Val Leu Ala Thr Thr Val 1 5 10 gtt tcc cccact aga gat cgt gca tct ggt gtc agg aat gtt gtt gca 96 Val Ser Pro ThrArg Asp Arg Ala Ser Gly Val Arg Asn Val Val Ala 15 20 25 30 aca agc tttcag act ctg acc cac gat gaa tgc tgt gca cac cct tcc 144 Thr Ser Phe GlnThr Leu Thr His Asp Glu Cys Cys Ala His Pro Ser 35 40 45 tgt tgg aag gccgaa gac ctg att tgt act aat caa cgt cgc agg acc 192 Cys Trp Lys Ala GluAsp Leu Ile Cys Thr Asn Gln Arg Arg Arg Thr 50 55 60 ctc tgaaccacgacctcgag 212 Leu 449 63 PRT Conus wittigi 449 Met Phe Thr Val Phe Leu LeuVal Val Leu Ala Thr Thr Val Val Ser 1 5 10 15 Pro Thr Arg Asp Arg AlaSer Gly Val Arg Asn Val Val Ala Thr Ser 20 25 30 Phe Gln Thr Leu Thr HisAsp Glu Cys Cys Ala His Pro Ser Cys Trp 35 40 45 Lys Ala Glu Asp Leu IleCys Thr Asn Gln Arg Arg Arg Thr Leu 50 55 60 450 25 PRT Conus wittigiPEPTIDE (1)..(25) Xaa at residues 2 and 13 is Glu or gamma-carboxy-Glu;Xaa at residue 7 is Pro or hydroxy-Pro; Xaa at residue 10 is Trp (D orL) or bromo-Trp (D or L) 450 Asp Xaa Cys Cys Ala His Xaa Ser Cys Xaa LysAla Xaa Asp Leu Ile 1 5 10 15 Cys Thr Asn Gln Arg Arg Arg Thr Leu 20 25451 230 DNA Conus caracteristicus CDS (7)..(195) 451 ggatcc atg ttc accgtg ttt ctg ttg gtt gtc ttg gca acc act gtc 48 Met Phe Thr Val Phe LeuLeu Val Val Leu Ala Thr Thr Val 1 5 10 gtt tcc ttc act tca gat cgt gcgtct gaa ggc agg aat gct gca gcc 96 Val Ser Phe Thr Ser Asp Arg Ala SerGlu Gly Arg Asn Ala Ala Ala 15 20 25 30 aag gac aaa gcg tct gac ctg gtggct ctg aga gtc agg gga tgc tgt 144 Lys Asp Lys Ala Ser Asp Leu Val AlaLeu Arg Val Arg Gly Cys Cys 35 40 45 gcc att cgt gaa tgt cgc ttg cag aatgca gcg tat tgt ggt gga ata 192 Ala Ile Arg Glu Cys Arg Leu Gln Asn AlaAla Tyr Cys Gly Gly Ile 50 55 60 tcc tgatgctcca ggaccctctg aaccacgacctcgag 230 Ser 452 63 PRT Conus caracteristicus 452 Met Phe Thr Val PheLeu Leu Val Val Leu Ala Thr Thr Val Val Ser 1 5 10 15 Phe Thr Ser AspArg Ala Ser Glu Gly Arg Asn Ala Ala Ala Lys Asp 20 25 30 Lys Ala Ser AspLeu Val Ala Leu Arg Val Arg Gly Cys Cys Ala Ile 35 40 45 Arg Glu Cys ArgLeu Gln Asn Ala Ala Tyr Cys Gly Gly Ile Ser 50 55 60 453 20 PRT Conuscaracteristicus PEPTIDE (1)..(20) Xaa at residue 7 is Glu orgamma-carboxy-Glu; Xaa at residue 15 is Tyr, 125I-Tyr, mono-iodo-Tyr,di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 453 Gly Cys Cys Ala Ile ArgXaa Cys Arg Leu Gln Asn Ala Ala Xaa Cys 1 5 10 15 Gly Gly Ile Ser 20 454568 DNA Conus betulinus CDS (395)..(508) misc_feature (1)..(568) n isunknown 454 agtaattnat atannagaaa gnaananaaa annatanaga atttaagtaatntaagaann 60 gaganagtga atagnagnta agtagannaa ganaggtaga nagnanangnggangntagn 120 taatagatan nntatngaga nattantagc ngtatanana agaaaagagggnaanngaaa 180 tgnngnaann ataantanta nngatngann ngnaagtgnn aagngtanaaggaanaacaa 240 antngttgtn taatntgnnt gngtgtgtnt gtgtgngtgt gtgtgtgtgngtgngtgtgt 300 ntgtgngnnt gtgtgngngn gngngtgtgt gtgtgngtgt gtgtgtgtgtgtgtgtgtgt 360 gtgtgtgtgt gngtgtgtgg ttctggatcc agca tct ggt ggc agg aaggct gca 415 Ser Gly Gly Arg Lys Ala Ala 1 5 gcc aaa gcg tct aac cgg atcgct ctg acc gtc agg agt gca aca tgc 463 Ala Lys Ala Ser Asn Arg Ile AlaLeu Thr Val Arg Ser Ala Thr Cys 10 15 20 tgt tat tat cct ccc tgt tac gaggct tat cca gaa agt tgt ctg 508 Cys Tyr Tyr Pro Pro Cys Tyr Glu Ala TyrPro Glu Ser Cys Leu 25 30 35 taacgtgaat catccagacc tttgtggctg aagaccctgatgctccaggg gcaagttcaa 568 455 38 PRT Conus betulinus 455 Ser Gly Gly ArgLys Ala Ala Ala Lys Ala Ser Asn Arg Ile Ala Leu 1 5 10 15 Thr Val ArgSer Ala Thr Cys Cys Tyr Tyr Pro Pro Cys Tyr Glu Ala 20 25 30 Tyr Pro GluSer Cys Leu 35 456 19 PRT Conus betulinus PEPTIDE (1)..(19) Xaa atresidues 12 and 16 is Glu or gamma-carboxy-Glu; Xaa at residues 8, 9 and13 is Pro or hydroxy-Pro; Xaa at residues 6, 7, 11 and 14 is Tyr,125I-Tyr, mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr 456Ser Ala Thr Cys Cys Xaa Xaa Xaa Xaa Cys Xaa Xaa Ala Xaa Xaa Xaa 1 5 1015 Ser Cys Leu 457 29 PRT Conus textile 457 Asp Cys Arg Gly Tyr Asp AlaPro Cys Ser Ser Gly Ala Pro Cys Cys 1 5 10 15 Asp Trp Trp Thr Cys SerAla Arg Thr Asn Arg Cys Phe 20 25 458 31 PRT Conus ammiralis 458 Gly MetTrp Gly Asp Cys Lys Asp Gly Leu Thr Thr Cys Phe Ala Pro 1 5 10 15 SerGlu Cys Cys Ser Glu Asp Cys Glu Gly Ser Cys Thr Met Trp 20 25 30 459 36PRT Conus ammiralis 459 Trp Arg Glu Gly Ser Cys Thr Ser Trp Leu Ala ThrCys Thr Gln Asp 1 5 10 15 Gln Gln Cys Cys Thr Asp Val Cys Tyr Lys ArgAsp Tyr Cys Ala Leu 20 25 30 Trp Asp Asp Arg 35 460 25 PRT Conusammiralis 460 Asn Cys Ser Asp Asp Trp Gln Tyr Cys Glu Ser Pro Ser AspCys Cys 1 5 10 15 Ser Trp Asp Cys Asp Val Val Cys Ser 20 25 461 39 PRTConus ammiralis 461 Trp Trp Arg Trp Gly Gly Cys Met Ala Trp Phe Gly LysCys Ser Lys 1 5 10 15 Asp Ser Glu Cys Cys Ser Asn Ser Cys Asp Ile ThrArg Cys Glu Leu 20 25 30 Met Arg Phe Pro Pro Asp Trp 35 462 29 PRT Conusammiralis 462 Asp Cys Arg Gly Tyr Asp Ala Pro Cys Ser Ser Gly Ala ProCys Cys 1 5 10 15 Asp Trp Trp Thr Cys Ser Ala Arg Thr Gly Arg Cys Phe 2025 463 26 PRT Conus ammiralis 463 Leu Cys Pro Asp Tyr Thr Glu Pro CysSer His Ala His Glu Cys Cys 1 5 10 15 Ser Trp Asn Cys His Asn Gly HisCys Thr 20 25 464 27 PRT Conus ammiralis 464 Cys Ser Ser Trp Ala Lys TyrCys Glu Val Asp Ser Glu Cys Cys Ser 1 5 10 15 Glu Gln Cys Val Arg SerTyr Cys Ala Met Trp 20 25 465 32 PRT Conus pennaceus PEPTIDE (1)..(32)Xaa at residues 14 and 26 is gamma-carboxy-Glu; Xaa at residue 31 ishydroxy-Pro 465 Asp Cys Thr Ser Trp Phe Gly Arg Cys Thr Val Asn Ser XaaCys Cys 1 5 10 15 Ser Asn Ser Cys Asp Gln Thr Tyr Cys Xaa Leu Tyr AlaPhe Xaa Ser 20 25 30 466 32 PRT Conus gloriamaris 466 Glu Cys Arg AlaTrp Tyr Ala Pro Cys Ser Pro Gly Ala Gln Cys Cys 1 5 10 15 Ser Leu LeuMet Cys Ser Lys Ala Thr Ser Arg Cys Ile Leu Ala Leu 20 25 30 467 27 PRTConus textile PEPTIDE (1)..(27) Xaa is gamma-carboxy-Glu 467 Cys Lys ThrTyr Ser Lys Tyr Cys Xaa Ala Asp Ser Xaa Cys Cys Thr 1 5 10 15 Xaa GlnCys Val Arg Ser Tyr Cys Thr Leu Phe 20 25 468 29 PRT Conus marmoreusPEPTIDE (1)..(29) Xaa is gamma-carboxy-Glu 468 Asn Gly Gln Cys Glu AspVal Trp Met Pro Cys Thr Ser Asn Trp Xaa 1 5 10 15 Cys Cys Ser Leu AspCys Glu Met Tyr Cys Thr Gln Ile 20 25 469 27 PRT Conus marmoreus PEPTIDE(1)..(27) Xaa is gamma-carboxy-Glu 469 Cys Gly Gly Trp Ser Thr Tyr CysGlu Val Asp Glu Xaa Cys Cys Ser 1 5 10 15 Glu Ser Cys Val Arg Ser TyrCys Thr Leu Phe 20 25 470 26 PRT Conus marmoreus PEPTIDE (1)..(26) Xaais gamma-carboxy-Glu 470 Asn Gly Gly Cys Lys Ala Thr Trp Met Ser Cys SerSer Gly Trp Xaa 1 5 10 15 Cys Cys Ser Met Ser Cys Asp Met Tyr Cys 20 25471 33 PRT Conus radiatus PEPTIDE (1)..(33) Xaa at residues 1, 10 and 33is bromo-Trp; Xaa at residues 5, 6, 15 and 26 is gamma-carboxy-Glu 471Xaa Phe Gly His Xaa Xaa Cys Thr Tyr Xaa Leu Gly Pro Cys Xaa Val 1 5 1015 Asp Asp Thr Cys Cys Ser Ala Ser Cys Xaa Ser Lys Phe Cys Gly Leu 20 2530 Xaa 472 31 PRT Conus radiatus 472 Trp Trp Glu Gly Glu Cys Ser Asn TrpLeu Gly Ser Cys Ser Thr Pro 1 5 10 15 Ser Asn Cys Cys Leu Lys Ser CysAsn Gly His Cys Thr Leu Trp 20 25 30 473 26 PRT Conus textile PEPTIDE(1)..(26) Xaa at residues 3 and 8 is hydroxy-Pro; Xaa at residues 7 and14 is gamma-carboxy-Glu 473 Leu Cys Xaa Asp Tyr Thr Xaa Xaa Cys Ser HisAla His Xaa Cys Cys 1 5 10 15 Ser Trp Asn Cys Tyr Asn Gly His Cys Thr 2025 474 28 PRT Conus textile 474 Asp Cys Tyr Ser Trp Leu Gly Ser Cys IleAla Pro Ser Gln Cys Cys 1 5 10 15 Ser Glu Val Cys Asp Tyr Tyr Cys ArgLeu Trp Arg 20 25 475 34 PRT Conus textile PEPTIDE (1)..(34) Xaa isgamma-carboxy-Glu 475 Trp Leu Glu Cys Ser Val Trp Phe Ser His Cys ThrLys Asp Ser Xaa 1 5 10 15 Cys Cys Ser Asn Ser Cys Asp Gln Thr Tyr CysThr Leu Met Pro Pro 20 25 30 Asp Trp 476 31 PRT Conus textile PEPTIDE(1)..(31) Xaa is gamma-carboxy-Glu 476 Gly Met Trp Gly Glu Cys Lys AspGly Leu Thr Thr Cys Leu Ala Pro 1 5 10 15 Ser Xaa Cys Cys Ser Glu AspCys Glu Gly Ser Cys Thr Met Trp 20 25 30 477 34 PRT Conus textilePEPTIDE (1)..(34) Xaa is gamma-carboxy-Glu 477 Asp Trp Trp Asp Asp GlyCys Ser Val Trp Gly Pro Cys Thr Val Asn 1 5 10 15 Ala Xaa Cys Cys SerGly Asp Cys His Glu Thr Cys Ile Phe Gly Trp 20 25 30 Glu Val 478 39 PRTConus textile PEPTIDE (1)..(39) Xaa is gamma-carboxy-Glu 478 Trp Trp ArgTrp Gly Gly Cys Met Ala Trp Phe Gly Leu Cys Ser Arg 1 5 10 15 Asp SerXaa Cys Cys Ser Asn Ser Cys Asp Val Thr Arg Cys Glu Leu 20 25 30 Met ProPhe Pro Pro Asp Trp 35 479 27 PRT Conus textile PEPTIDE (1)..(27) Xaa isgamma-carboxy-Glu 479 Cys Gly Gly Tyr Ser Thr Tyr Cys Xaa Val Asp SerXaa Cys Cys Ser 1 5 10 15 Asp Asn Cys Val Arg Ser Tyr Cys Thr Leu Phe 2025 480 43 PRT Conus caracteristicus 480 Gly Cys Ser Gly Thr Cys His ArgArg Glu Asp Gly Lys Cys Arg Gly 1 5 10 15 Thr Cys Asp Cys Ser Gly TyrSer Tyr Cys Arg Cys Gly Asp Ala His 20 25 30 His Phe Tyr Arg Gly Cys ThrCys Ser Cys Gln 35 40 481 42 PRT Conus caracteristicus 481 Gly Cys SerGly Thr Cys His Arg Arg Glu Asp Gly Lys Cys Arg Gly 1 5 10 15 Thr CysAsp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly Asp Ala His 20 25 30 His PheTyr Arg Gly Cys Thr Cys Thr Cys 35 40 482 42 PRT Conus caracteristicus482 Gly Cys Ser Gly Thr Cys Arg Arg His Arg Asp Gly Lys Cys Arg Gly 1 510 15 Thr Cys Asp Cys Ser Gly Tyr Ser Tyr Cys Arg Cys Gly Asp Ala His 2025 30 His Phe Tyr Arg Gly Cys Thr Cys Thr Cys 35 40 483 42 PRT Conuscaracteristicus 483 Gly Cys Ser Gly Thr Cys Arg Arg His Arg Asp Gly LysCys Arg Gly 1 5 10 15 Thr Cys Asp Cys Ser Gly Tyr Ser Tyr Cys Arg CysGly Asp Ala His 20 25 30 His Phe Tyr Arg Gly Cys Thr Cys Thr Cys 35 40484 42 PRT Conus caracteristicus 484 Gly Cys Ser Gly Thr Cys His Arg ArgGlu Asp Gly Lys Cys Arg Gly 1 5 10 15 Thr Cys Asp Cys Ser Gly Tyr SerTyr Cys Arg Cys Gly Asp Ala His 20 25 30 His Phe Tyr Arg Gly Cys Thr CysThr Cys 35 40 485 42 PRT Conus caracteristicus 485 Gly Cys Ser Gly ThrCys His Arg Arg Gln Asn Gly Glu Cys Gln Gly 1 5 10 15 Thr Cys Asp CysAsp Gly His Asp His Cys Asp Cys Gly Asp Thr Leu 20 25 30 Gly Thr Tyr SerGly Cys Val Cys Ile Cys 35 40 486 45 PRT Conus lacterculatus 486 Gln SerGlu Thr Ala Cys Arg Ser Leu Gly Ser Tyr Gln Cys Met Gly 1 5 10 15 LysCys Gln Leu Gly Val His Ser Trp Cys Glu Cys Ile Tyr Asn Arg 20 25 30 GlySer Gln Lys Ser Gly Cys Ala Cys Arg Cys Gln Lys 35 40 45 487 48 PRTConus monachus 487 Gln Cys Thr Leu Val Asn Asn Cys Asp Arg Asn Gly GluArg Ala Cys 1 5 10 15 Asn Gly Asp Cys Ser Cys Glu Gly Gln Ile Cys LysCys Gly Tyr Arg 20 25 30 Val Ser Pro Gly Lys Ser Gly Cys Ala Cys Thr CysArg Asn Ala Lys 35 40 45 488 41 PRT Conus purpurascens 488 Gly Cys SerGly Ser Pro Cys Phe Lys Asn Lys Thr Cys Arg Asp Glu 1 5 10 15 Cys IleCys Gly Gly Leu Ser Asn Cys Trp Cys Gly Tyr Gly Gly Ser 20 25 30 Arg GlyCys Lys Cys Thr Cys Arg Glu 35 40 489 47 PRT Conus radiatus 489 Lys CysAsn Phe Asp Lys Cys Lys Gly Thr Gly Val Tyr Asn Cys Gly 1 5 10 15 GluSer Cys Ser Cys Glu Gly Leu His Ser Cys Arg Cys Thr Tyr Asn 20 25 30 IleGly Ser Met Lys Ser Gly Cys Ala Cys Ile Cys Thr Tyr Tyr 35 40 45 490 44PRT Conus radiatus 490 Tyr Gly Leu Gly Cys Ala Gly Thr Cys Gly Ser SerSer Asn Cys Val 1 5 10 15 Arg Asp Tyr Cys Asp Cys Pro Lys Pro Asn CysTyr Cys Thr Gly Lys 20 25 30 Gly Phe Arg Gln Pro Gly Cys Gly Cys Ser CysLeu 35 40 491 46 PRT Conus striolatus 491 Gln Cys Thr Phe Val Asn AsnCys Gln Gln Asn Gly Cys Ala Asn Gly 1 5 10 15 Asp Cys Ser Cys Gly AspGln Ile Cys Lys Cys Gly Tyr Arg Ile Ser 20 25 30 Pro Gly Arg Ser Gly CysAla Cys Thr Cys Arg Asn Ala Lys 35 40 45 492 44 PRT Conus tulipa 492 PheGly Pro Ile Cys Thr Cys Phe Lys Ser Gln Asn Cys Arg Gly Ser 1 5 10 15Cys Glu Cys Met Ser Pro Pro Gly Cys Tyr Cys Ser Asn Asn Gly Ile 20 25 30Arg Glu Arg Gly Cys Ser Cys Thr Cys Pro Gly Thr 35 40 493 46 PRT Conustulipa 493 Gly Cys Thr Gly Asn Cys Asp Trp Thr Cys Ser Gly Asp Cys SerCys 1 5 10 15 Gln Gly Thr Ser Asp Ser Cys His Cys Ile Pro Pro Lys SerIle Gly 20 25 30 Asn Arg Cys Arg Cys Gln Cys Lys Arg Lys Ile Glu Ile Asp35 40 45 494 15 PRT Conus textile 494 Glu Cys Cys Glu Asp Gly Trp CysCys Thr Ala Ala Pro Leu Thr 1 5 10 15 495 15 PRT Conus textile 495 GlyCys Cys Glu Asp Gly Trp Cys Cys Thr Ala Ala Pro Leu Thr 1 5 10 15 496 20PRT Conus marmoreus 496 Asn Gly Cys Cys Arg Ala Gly Asp Cys Cys Ser ArgPhe Glu Ile Lys 1 5 10 15 Glu Asn Asp Phe 20 497 11 PRT Conus marmoreus497 Asn Gly Cys Cys Arg Ala Gly Asp Cys Cys Ser 1 5 10 498 10 PRT Conusmarmoreus 498 Asn Ala Cys Cys Ile Val Arg Gln Cys Cys 1 5 10 499 10 PRTConus quercinus 499 Gly Cys Cys Ala Arg Leu Thr Cys Cys Val 1 5 10 50010 PRT Conus quercinus 500 Gly Cys Cys Ala Met Leu Thr Cys Cys Val 1 510 501 12 PRT Conus purpurascens 501 Gly Cys Cys Pro Lys Gln Met Arg CysCys Thr Leu 1 5 10 502 11 PRT Conus caracteristicus 502 Cys Cys Pro ArgArg Leu Ala Cys Cys Ile Ile 1 5 10 503 10 PRT Conus caracteristicus 503Cys Cys Pro Asn Lys Pro Cys Cys Phe Ile 1 5 10 504 13 PRT Conusgeographus 504 Glx Gly Trp Cys Cys Lys Glu Asn Ile Ala Cys Cys Ile 1 510 505 13 PRT Conus geographus 505 Glx Gly Trp Cys Cys Lys Glu Asn IleAla Cys Cys Val 1 5 10 506 15 PRT Conus imperialis 506 Asp Trp Asn SerCys Cys Gly Lys Asn Pro Gly Cys Cys Pro Trp 1 5 10 15 507 11 PRT Conusbetulinus 507 Asn Cys Cys Pro Asp Ser Pro Pro Cys Cys His 1 5 10 508 12PRT Conus ammiralis 508 Gly Asn Cys Cys Glu Phe Trp Glu Phe Cys Cys Asp1 5 10 509 9 PRT Conus dalli 509 Cys Cys Glu Tyr Trp Lys Leu Cys Cys 1 5510 11 PRT Conus omaria 510 Val Cys Cys Gly Tyr Lys Phe Phe Cys Cys Arg1 5 10 511 11 PRT Conus aulicus 511 Phe Cys Cys Pro Val Ile Arg Tyr CysCys Trp 1 5 10 512 11 PRT Conus aulicus 512 Phe Cys Cys Pro Phe Ile ArgTyr Cys Cys Trp 1 5 10 513 10 PRT Conus aulicus 513 Cys Cys Pro Met IleTyr Trp Cys Cys Ser 1 5 10 514 10 PRT Conus aulicus 514 Cys Cys Pro GluIle Tyr Trp Cys Cys Ser 1 5 10 515 10 PRT Conus nobilis 515 Ile Cys CysPro Ile Ile Leu Trp Cys Cys 1 5 10 516 9 PRT Conus ammiralis 516 Cys CysPro Pro Val Ile Trp Cys Cys 1 5 517 11 PRT Conus textile 517 Cys Cys GlnThr Phe Tyr Trp Cys Cys Val Gln 1 5 10 518 13 PRT Conus aulicus 518 TrpAsn Asn Tyr Cys Cys Thr Asn Glu Leu Trp Cys Cys 1 5 10 519 13 PRT Conusgloriamaris 519 Leu Cys Cys Val Thr Glu Asp Trp Cys Cys Glu Trp Trp 1 510 520 11 PRT Conus gloriamaris 520 Val Cys Cys Arg Pro Val Gln Asp CysCys Ser 1 5 10 521 13 PRT Conus dalli 521 Pro Val Asn Cys Cys Pro IleAsp Gln Ser Cys Cys Ser 1 5 10 522 17 PRT Conus spurius 522 Gly Asn IleHis Cys Cys Thr Lys Tyr Gln Pro Cys Cys Ser Ser Pro 1 5 10 15 Ser 523 12PRT Conus textile 523 Glx Thr Cys Cys Gly Tyr Arg Met Cys Val Pro Cys 15 10 524 11 PRT Conus bandanus 524 Ala Cys Cys Gly Tyr Lys Leu Cys SerPro Cys 1 5 10 525 13 PRT Conus pennaceus 525 Ser Thr Cys Cys Gly PheLys Met Cys Ile Pro Cys Arg 1 5 10 526 13 PRT Conus pennaceus 526 SerThr Cys Cys Gly Phe Lys Met Cys Ile Pro Cys Ser 1 5 10 527 12 PRT Conuspennaceus 527 Ser Thr Cys Cys Gly Phe Lys Met Cys Ile Pro Cys 1 5 10 52812 PRT Conus episcopatus 528 Ser Thr Cys Cys Gly Tyr Arg Met Cys Val ProCys 1 5 10 529 13 PRT Conus marmoreus 529 Asn Gly Val Cys Cys Gly TyrLys Leu Cys Leu Pro Cys 1 5 10 530 12 PRT Conus pennaceus 530 Leu CysCys Gly Phe Trp Met Cys Ile Pro Cys Asn 1 5 10 531 13 PRT Conusmarmoreus PEPTIDE (1)..(13) Xaa is hydroxy-Pro 531 Asn Gly Val Cys CysGly Tyr Lys Leu Cys His Xaa Cys 1 5 10 532 12 PRT Conus marmoreusPEPTIDE (1)..(12) Xaa is hydroxy-Pro 532 Gly Val Cys Cys Gly Tyr Lys LeuCys His Xaa Cys 1 5 10 533 11 PRT Conus bandanus 533 Ala Cys Cys Gly TyrLys Leu Cys Ser Pro Cys 1 5 10 534 12 PRT Conus aulicus 534 Ser Val CysCys Gly Tyr Lys Leu Cys Phe Pro Cys 1 5 10 535 13 PRT Conus textile 535Asn Gly Val Cys Cys Gly Tyr Arg Met Cys Val Pro Cys 1 5 10 536 12 PRTConus textile 536 Glx Thr Cys Cys Gly Tyr Arg Met Cys Val Pro Cys 1 5 10537 12 PRT Conus ammiralis 537 Glx Ala Cys Cys Gly Phe Lys Met Cys ValPro Cys 1 5 10 538 13 PRT Conus pennaceus 538 Ser Thr Cys Cys Gly PheLys Met Cys Ile Pro Cys Arg 1 5 10 539 14 PRT Conus pennaceus 539 AsnGly Val Cys Cys Gly Phe Trp Met Cys Ile Pro Cys Asn 1 5 10 540 12 PRTConus omaria 540 Asp Val Cys Cys Tyr Val Arg Met Cys Pro Cys Arg 1 5 10541 7 PRT Conus imperialis 541 Glx Cys Gly Gln Ala Trp Cys 1 5 542 8 PRTConus stercusmuscarum PEPTIDE (1)..(8) Xaa is hydroxy-Pro 542 Gly CysXaa Trp Gln Pro Val Cys 1 5 543 11 PRT Conus arenatus PEPTIDE (1)..(11)Xaa is hydroxy-Pro 543 Glx Tyr Gly Cys Xaa Xaa Gly Leu Trp Cys His 1 510 544 10 PRT Conus arenatus 544 Ala Ser Gly Cys Pro Trp Arg Pro Trp Cys1 5 10 545 11 PRT Conus arenatus 545 Glx Tyr Gly Cys Pro Val Gly Leu TrpCys Asp 1 5 10 546 9 PRT Conus arenatus 546 Ser Gly Cys Pro Trp Gln ProTrp Cys 1 5 547 9 PRT Conus arenatus 547 Ser Gly Cys Pro Trp His Pro TrpCys 1 5 548 30 PRT Conus ermineus PEPTIDE (1)..(30) Xaa is hydroxy-Pro548 Gly Cys Cys Gly Lys Tyr Xaa Asn Ala Ala Cys His Xaa Cys Gly Cys 1 510 15 Thr Val Gly Arg Xaa Xaa Tyr Cys Asp Arg Xaa Ser Gly Gly 20 25 30549 25 PRT Conus purpurascens 549 Gly Cys Cys Gly Ser Tyr Pro Asn AlaAla Cys His Pro Cys Gly Cys 1 5 10 15 Lys Asp Arg Pro Ser Tyr Cys GlyGln 20 25 550 23 PRT Conus purpurascens 550 Glu Gly Cys Cys Ser Asn ProAla Cys His Pro Cys Gly Cys Lys Asp 1 5 10 15 Arg Pro Ser Tyr Cys GlyGln 20 551 32 PRT Conus arenatus 551 Val Val Thr Glu Ala Cys Glu Glu SerCys Glu Glu Glu Glu Lys His 1 5 10 15 Cys Cys His Val Asn Asn Gly ValPro Ser Cys Ala Val Ile Cys Trp 20 25 30 552 32 PRT Conus arenatus 552Ile Val Thr Glu Ala Cys Glu Glu Ser Cys Glu Asp Glu Glu Lys His 1 5 1015 Cys Cys His Val Asn Asn Gly Val Pro Ser Cys Ala Val Ile Cys Trp 20 2530 553 32 PRT Conus arenatus 553 Ile Val Thr Glu Ala Cys Glu Glu His CysGlu Asp Glu Glu Gln Phe 1 5 10 15 Cys Cys Gly Leu Glu Asn Gly Gln ProPhe Cys Ala Pro Val Cys Phe 20 25 30 554 32 PRT Conus arenatus 554 ValVal Thr Gly Ala Cys Glu Glu His Cys Glu Asp Glu Glu Lys His 1 5 10 15Cys Cys Gly Leu Glu Asn Gly Gln Pro Phe Cys Ala Arg Leu Cys Leu 20 25 30555 32 PRT Conus distans 555 Asn Val Asp Gln Glu Cys Ile Asp Ala Cys GlnLeu Glu Asp Lys Asn 1 5 10 15 Cys Cys Gly Arg Thr Asp Gly Glu Pro ArgCys Ala Lys Ile Cys Leu 20 25 30 556 32 PRT Conus distans 556 Glu ThrAsp Gln Glu Cys Ile Asp Ile Cys Lys Gln Glu Asp Lys Lys 1 5 10 15 CysCys Gly Arg Ser Asn Gly Glu Pro Thr Cys Ala Lys Ile Cys Leu 20 25 30 55732 PRT Conus distans 557 Glu Thr Asp Gln Glu Cys Ile Asp Thr Cys Glu GlnGlu Asp Lys Lys 1 5 10 15 Cys Cys Gly Arg Thr Asn Gly Glu Pro Val CysAla Lys Ile Cys Phe 20 25 30 558 37 PRT Conus purpurascens 558 Pro LysThr Glu Ala Cys Glu Glu Val Cys Glu Leu Glu Glu Lys His 1 5 10 15 CysCys Cys Ile Arg Ser Asp Gly Pro Lys Cys Ser Arg Lys Cys Leu 20 25 30 LeuSer Ile Phe Cys 35 559 32 PRT Conus purpurascens 559 Val Val Ser Glu GluCys Lys Lys Tyr Cys Lys Lys Gln Asn Lys Asn 1 5 10 15 Cys Cys Ser SerLys His Glu Glu Pro Arg Cys Ala Lys Ile Cys Phe 20 25 30 560 32 PRTConus sponsalis 560 Ala Val Thr Glu Ala Cys Thr Glu Asp Cys Lys Thr GlnAsp Lys Lys 1 5 10 15 Cys Cys Gly Glu Met Asn Gly Gln His Thr Cys AlaLys Ile Cys Leu 20 25 30 561 37 PRT Conus tulipa 561 Pro Lys Thr Lys GluCys Glu Arg Tyr Cys Glu Leu Glu Glu Lys His 1 5 10 15 Cys Cys Cys IleArg Ser Asn Gly Pro Lys Cys Ser Arg Ile Cys Ile 20 25 30 Phe Lys Phe TrpCys 35 562 36 PRT Conus tulipa 562 Pro Lys Thr Arg Glu Cys Glu Met GlnCys Glu Gln Glu Glu Lys His 1 5 10 15 Cys Cys Arg Val Arg Asp Gly ThrGly Gln Cys Ala Pro Lys Cys Leu 20 25 30 Gly Ile Asn Trp 35 563 9 PRTConus geographus 563 Cys Phe Ile Arg Asn Cys Pro Lys Gly 1 5 564 9 PRTConus striatus 564 Cys Ile Ile Arg Asn Cys Pro Arg Gly 1 5 565 28 PRTConus arenatus 565 Gly Cys Thr Pro Pro Gly Gly Val Cys Gly Tyr His GlyHis Cys Cys 1 5 10 15 Asp Phe Cys Asp Thr Phe Gly Asn Leu Cys Val Ser 2025 566 27 PRT Conus geographus 566 Ala Cys Ser Gly Arg Gly Ser Arg CysPro Pro Gln Cys Cys Met Gly 1 5 10 15 Leu Thr Cys Gly Arg Glu Tyr ProPro Arg Cys 20 25 567 31 PRT Conus caracteristicus 567 Asn Cys Gly GluGln Gly Glu Gly Cys Ala Thr Arg Pro Cys Cys Ser 1 5 10 15 Gly Leu SerCys Val Gly Ser Arg Pro Gly Gly Leu Cys Gln Tyr 20 25 30 568 23 PRTConus textile 568 Asn Cys Pro Tyr Cys Val Val Tyr Cys Cys Pro Pro AlaTyr Cys Glu 1 5 10 15 Ala Ser Gly Cys Arg Pro Pro 20 569 27 PRT Conusdelesstii PEPTIDE (1)..(27) Xaa at residue 4 is hydroxy-Pro; Xaa atresidues 13 and 16 is gamm-carboxy-Glu 569 Ala Cys Lys Xaa Lys Asn AsnLeu Cys Ala Ile Thr Xaa Met Ala Xaa 1 5 10 15 Cys Cys Ser Gly Phe CysLeu Ile Tyr Arg Cys 20 25 570 30 PRT Conus lividus 570 Ser Cys Gly HisSer Gly Ala Gly Cys Tyr Thr Arg Pro Cys Cys Pro 1 5 10 15 Gly Leu HisCys Ser Gly Gly Gln Ala Gly Gly Leu Cys Val 20 25 30 571 35 PRT Conuslividus 571 Asp Cys Gly Glu Ser Gly Gln Gly Cys Tyr Ser Val Arg Pro CysCys 1 5 10 15 Pro Gly Leu Ile Cys Lys Gly Thr Gly Gly Gly Gly Leu CysArg Pro 20 25 30 Ser Gly Ile 35 572 31 PRT Conus miliaris 572 Cys ThrPro Pro Gly Gly Leu Cys Tyr His Ala Tyr Pro Cys Cys Ser 1 5 10 15 LysThr Cys Asn Leu Asp Thr Ser Gln Cys Glu Pro Arg Trp Ser 20 25 30 573 36PRT Conus miles 573 Cys Thr Asp Asp Ser Gln Phe Cys Asn Pro Ser Asn HisAsp Cys Cys 1 5 10 15 Ser Gly Lys Cys Ile Asp Glu Gly Asp Asn Gly IleCys Ala Ile Val 20 25 30 Pro Glu Asn Ser 35 574 36 PRT Conus miles 574Cys Thr Glu Asp Ser Gln Phe Cys Asn Pro Ser Asn His Asp Cys Cys 1 5 1015 Ser Gly Lys Cys Ile Asp Glu Gly Asp Asn Gly Ile Cys Ala Ile Val 20 2530 Pro Glu Asn Ser 35 575 30 PRT Conus pulicarius 575 Cys Ser Asp PheGly Ser Asp Cys Val Pro Ala Thr His Asn Cys Cys 1 5 10 15 Ser Gly GluCys Phe Gly Phe Glu Asp Phe Gly Leu Cys Thr 20 25 30 576 30 PRT Conusquercinus 576 Ala Cys Ser Gln Val Gly Glu Ala Cys Phe Pro Gln Lys ProCys Cys 1 5 10 15 Pro Gly Phe Leu Cys Asn His Ile Gly Gly Met Cys HisHis 20 25 30 577 28 PRT Conus striatus 577 Cys Leu Pro Asp Gly Thr SerCys Leu Phe Ser Arg Ile Arg Cys Cys 1 5 10 15 Gly Thr Cys Ser Ser IleLeu Lys Ser Cys Val Ser 20 25 578 31 PRT Conus tessulatus 578 Ser CysAla Glu Phe Gly Glu Val Cys Ser Ser Thr Ala Cys Cys Pro 1 5 10 15 AspLeu Asp Cys Val Glu Ala Tyr Ser Pro Ile Cys Leu Trp Glu 20 25 30 579 27PRT Conus textile 579 Lys Cys Val Glu Gln Trp Lys Tyr Cys Thr Arg GluSer Leu Cys Cys 1 5 10 15 Ala Gly Leu Cys Leu Phe Ser Phe Cys Ile Leu 2025 580 29 PRT Conus textile 580 Cys Val Glu Gln Trp Glu Val Cys Gly IleIle Leu Phe Ser Ser Ser 1 5 10 15 Cys Cys Gly Gln Leu Cys Leu Phe GlyPhe Cys Val Leu 20 25 581 32 PRT Conus virgo 581 Asp Cys Gly Gly Gln GlyGlu Gly Cys Tyr Thr Gln Pro Cys Cys Pro 1 5 10 15 Gly Leu Arg Cys ArgGly Gly Gly Thr Gly Gly Gly Val Cys Gln Leu 20 25 30 582 33 PRT Conuswittigi 582 Phe Gly Ser Phe Ile Pro Cys Ala Arg Leu Gly Glu Pro Cys ThrIle 1 5 10 15 Cys Cys Arg Pro Leu Arg Cys Arg Glu Ser Gly Thr Pro ThrCys Gln 20 25 30 Val 583 30 PRT Conus regius 583 Thr Cys Leu Glu His AsnLys Leu Cys Trp Tyr Asp Arg Asp Cys Cys 1 5 10 15 Thr Ile Tyr Cys AsnGlu Asn Lys Cys Gly Val Lys Pro Gln 20 25 30 584 34 PRT Conus radiatus584 Ala Cys Lys Ser Asn Tyr Asp Cys Pro Gln Arg Phe Lys Cys Cys Ser 1 510 15 Tyr Thr Trp Asn Gly Ser Ser Gly Tyr Cys Lys Arg Val Cys Tyr Leu 2025 30 Tyr Arg 585 24 PRT Conus purpurascens PEPTIDE (1)..(24) Xaa ishydroxy-Pro 585 Gly Xaa Xaa Cys Cys Leu Tyr Gly Ser Cys Arg Xaa Phe XaaGly Cys 1 5 10 15 Tyr Asn Ala Leu Cys Cys Arg Lys 20 586 24 PRT Conuspurpurascens 586 His Pro Pro Cys Cys Met Tyr Gly Arg Cys Arg Arg Tyr ProGly Cys 1 5 10 15 Ser Ser Ala Ser Cys Cys Gln Gly 20 587 36 PRT Conusconsors 587 Ala Pro Glu Leu Val Val Thr Ala Thr Thr Thr Cys Cys Gly TyrAsp 1 5 10 15 Pro Met Thr Ile Cys Pro Pro Cys Met Cys Thr His Ser CysPro Pro 20 25 30 Lys Arg Lys Pro 35 588 39 PRT Conus aurisiacus 588 GlxSer Trp Leu Val Pro Ser Thr Ile Thr Thr Cys Cys Gly Tyr Asp 1 5 10 15Pro Gly Thr Met Cys Pro Pro Cys Arg Cys Asn Asn Thr Cys Lys Pro 20 25 30Lys Lys Pro Lys Pro Gly Lys 35 589 37 PRT Conus consors 589 Ala Pro GluLeu Val Val Thr Ala Thr Thr Thr Cys Cys Gly Tyr Asp 1 5 10 15 Pro MetThr Trp Cys Pro Ser Cys Met Cys Thr Tyr Ser Cys Pro His 20 25 30 Gln ArgLys Lys Pro 35 590 36 PRT Conus magus 590 Ala Pro Glu Leu Val Val ThrAla Thr Thr Thr Cys Cys Gly Tyr Asp 1 5 10 15 Pro Met Thr Ile Cys ProPro Cys Met Cys Thr His Ser Cys Pro Pro 20 25 30 Lys Gly Lys Pro 35 59138 PRT Conus aurisiacus 591 Glx Lys Trp Leu Val His Ser Lys Ile Thr TyrCys Cys Gly Tyr Asn 1 5 10 15 Lys Met Asp Met Cys Pro Pro Cys Met CysThr Tyr Ser Cys Pro Pro 20 25 30 Leu Lys Lys Lys Arg Pro 35 592 29 PRTConus aurisiacus 592 Ala Pro Trp Thr Val Val Thr Ala Thr Thr Asn Cys CysGly Ile Thr 1 5 10 15 Gly Pro Gly Cys Leu Pro Cys Arg Cys Thr Gln ThrCys 20 25 593 13 PRT Conus geographus 593 Glu Cys Cys His Pro Ala CysGly Lys His Tyr Ser Cys 1 5 10 594 13 PRT Conus geographus 594 Glu CysCys Asn Pro Ala Cys Gly Arg His Phe Ser Cys 1 5 10 595 22 PRT Conusstriatus 595 Ala Tyr Cys Cys His Pro Ala Cys Gly Pro Asn Tyr Ser Cys GlyThr 1 5 10 15 Ser Cys Ser Arg Thr Leu 20 596 14 PRT Conus striatus 596Ala Tyr Cys Cys His Pro Val Cys Gly Lys Asn Phe Asp Cys 1 5 10 597 22PRT Conus rattus 597 Gly Cys Cys Cys Asn Pro Ala Cys Gly Pro Asn Tyr GlyCys Gly Thr 1 5 10 15 Ser Cys Ser Arg Thr Leu 20 598 22 PRT Conusarenatus 598 Glx Asp Tyr Cys Cys Thr Ile Pro Ser Cys Trp Asp Arg Tyr LysGlu 1 5 10 15 Arg Cys Arg His Ile Arg 20 599 22 PRT Conus eburneus 599Glx Asp Tyr Cys Cys Thr Ile Pro Ser Cys Trp Asp Arg Tyr Lys Glu 1 5 1015 Arg Cys Arg His Ile Arg 20 600 13 PRT Conus miles 600 Asp Tyr Cys CysHis Arg Gly Pro Cys Met Val Trp Cys 1 5 10 601 13 PRT Conus jDedius 601Gly Cys Cys Ser Asp Pro Arg Cys Arg Tyr Arg Cys Arg 1 5 10 602 17 PRTConus omaria 602 Gly Cys Cys Ser His Pro Ala Cys Asn Val Asn Asn Pro HisIle Cys 1 5 10 15 Gly 603 17 PRT Conus omaria 603 Gly Cys Cys Ser HisPro Ala Cys Asn Val Asn Asn Pro His Ile Cys 1 5 10 15 Gly 604 19 PRTConus quercinus 604 Glx Gly Cys Cys Ser Asp Pro Ala Cys Ala Val Ser AsnPro Asp Ile 1 5 10 15 Cys Gly Gly 605 17 PRT Conus bandanus 605 Pro GluCys Cys Thr His Pro Ala Cys His Val Ser His Pro Glu Leu 1 5 10 15 Cys606 17 PRT Conus marmoreus 606 Pro Glu Cys Cys Thr His Pro Ala Cys HisVal Ser Asn Pro Glu Leu 1 5 10 15 Cys 607 15 PRT Conus miles 607 Cys CysAsn His Pro Ala Cys Ala Gly Lys Asn Ser Asp Leu Cys 1 5 10 15 608 16 PRTConus magus 608 Gly Cys Cys Tyr His Pro Thr Cys His Leu Glu His Ser AsnLeu Cys 1 5 10 15 609 17 PRT Conus nobilis 609 Gly Cys Cys Glu Arg ProPro Cys Arg Trp Gln Asn Pro Asp Leu Cys 1 5 10 15 Gly 610 17 PRT Conusatlanticus 610 Thr Cys Cys Ser Arg Pro Thr Cys Arg Met Glu Tyr Pro GluLeu Cys 1 5 10 15 Gly 611 21 PRT Conus quercinus 611 Asn Glu Cys Cys AspAsn Pro Pro Cys Lys Ser Ser Asn Pro Asp Leu 1 5 10 15 Cys Asp Trp ArgSer 20 612 16 PRT Conus leopardus 612 Cys Cys Ser Asn Pro Ala Cys AsnArg Tyr Asn Pro Ala Ile Cys Asp 1 5 10 15 613 17 PRT Conus emaciatus 613Asp Cys Cys Asn Phe Pro Ala Cys Ala Ala Ser Asn Pro Gly Leu Cys 1 5 1015 Thr 614 14 PRT Conus victor 614 Cys Cys Ser Ser Pro Pro Cys Phe AlaSer Asn Pro Ala Cys 1 5 10 615 17 PRT Conus cinereus gubba 615 Gly GlyCys Cys Ser Phe Pro Pro Cys Ile Ala Asn Asn Pro Phe Cys 1 5 10 15 Ala616 15 PRT Conus flavidus 616 Gly Cys Cys Ser Asn Pro Pro Cys Ser TyrLeu Asn Pro Ala Cys 1 5 10 15 617 16 PRT Conus emaciatus 617 Asp Cys CysSer Asp Pro Pro Cys Ala His Asn Asn Pro Asp Cys Arg 1 5 10 15 618 17 PRTConus generalis 618 Gly Cys Cys Ser Asn Pro Pro Cys Tyr Ala Asn Asn GlnAla Tyr Cys 1 5 10 15 Asn 619 25 PRT Conus wittigi 619 Asp Glu Cys CysAla His Pro Ser Cys Trp Lys Ala Glu Asp Leu Ile 1 5 10 15 Cys Thr AsnGln Arg Arg Arg Thr Leu 20 25 620 20 PRT Conus caracteristicus 620 GlyCys Cys Ala Ile Arg Glu Cys Arg Leu Gln Asn Ala Ala Tyr Cys 1 5 10 15Gly Gly Ile Ser 20 621 19 PRT Conus betulinus 621 Ser Ala Thr Cys CysTyr Tyr Pro Pro Cys Tyr Glu Ala Tyr Pro Glu 1 5 10 15 Ser Cys Leu 622 6PRT Conus magus PEPTIDE (1)..(6) Xaa is gamma-carboxy-Glu 622 Val TyrXaa Thr His Pro 1 5 623 14 PRT Conus striatus 623 Trp Ser Trp Arg MetGly Asn Gly Asp Arg Arg Ser Asp Gln 1 5 10 624 11 PRT Conus quercinus624 Asp Cys Gln Pro Cys Gly His Asn Val Cys Cys 1 5 10 625 29 PRT Conusgeographus PEPTIDE (1)..(29) Xaa is gamma-carboxy-Glu 625 Lys Phe LeuSer Gly Gly Phe Lys Xaa Ile Val Cys His Arg Tyr Cys 1 5 10 15 Ala LysGly Ile Ala Lys Glu Phe Cys Asn Cys Pro Asp 20 25 626 6 PRT Conus magus626 Arg Pro Lys Asn Ser Trp 1 5 627 7 PRT Conus magus PEPTIDE (1)..(7)Xaa is hydroxy-Pro 627 Ala Arg Xaa Lys Asn Ser Trp 1 5 628 6 PRT Conusmagus PEPTIDE (1)..(6) Xaa is hydroxy-Pro 628 Arg Xaa Lys Asn Ser Trp 15 629 71 PRT Conus geographus 629 Cys Cys Pro Ser Ser Lys Glu Asp SerLeu Asn Cys Ile Glu Thr Met 1 5 10 15 Ala Thr Thr Ala Thr Cys Met LysSer Asn Lys Gly Glu Ile Tyr Ser 20 25 30 Tyr Ala Cys Gly Tyr Cys Gly LysLys Lys Glu Ser Cys Phe Gly Asp 35 40 45 Lys Lys Pro Val Thr Asp Tyr GlnCys Gln Thr Arg Asn Ile Pro Asn 50 55 60 Pro Cys Gly Gly Ala Ala Leu 6570 630 65 PRT Conus geographus 630 Asp Glu Ser Lys Cys Asp Arg Cys AsnCys Ala Glu Leu Arg Ser Ser 1 5 10 15 Arg Cys Thr Gln Ala Ile Phe CysLeu Thr Pro Glu Leu Cys Thr Pro 20 25 30 Ser Ile Ser Cys Pro Thr Gly GluCys Arg Cys Thr Lys Phe His Gln 35 40 45 Ser Arg Cys Thr Arg Phe Val GluCys Val Pro Asn Lys Cys Arg Asp 50 55 60 Ala 65 631 60 PRT Conusgeographus 631 Asp Asp Ser Tyr Cys Asp Gly Cys Leu Cys Thr Ile Leu LysLys Glu 1 5 10 15 Thr Cys Thr Ser Thr Met Ser Cys Arg Gly Thr Cys ArgLys Glu Trp 20 25 30 Pro Cys Trp Glu Glu Asp Cys Tyr Cys Thr Glu Ile GlnGly Gly Ala 35 40 45 Cys Val Thr Pro Ser Glu Cys Lys Pro Gly Glu Cys 5055 60 632 43 PRT Conus radiatus 632 Gly Cys Val Tyr Glu Gly Ile Glu TyrSer Val Gly Glu Thr Tyr Gln 1 5 10 15 Ala Asp Cys Asn Thr Cys Arg CysAsp Gly Phe Asp Leu Ala Thr Cys 20 25 30 Thr Val Ala Gly Cys Thr Gly PheGly Pro Glu 35 40 633 86 PRT Conus striatus 633 Ser Gly Pro Ala Asp CysCys Arg Met Lys Glu Cys Cys Thr Asp Arg 1 5 10 15 Val Asn Glu Cys LeuGln Arg Tyr Ser Gly Arg Glu Asp Lys Phe Val 20 25 30 Ser Phe Cys Tyr GlnGlu Ala Thr Val Thr Cys Gly Ser Phe Asn Glu 35 40 45 Ile Val Gly Cys CysTyr Gly Tyr Gln Met Cys Met Ile Arg Val Val 50 55 60 Lys Pro Asn Ser LeuSer Gly Ala His Glu Ala Cys Lys Thr Val Ser 65 70 75 80 Cys Gly Asn ProCys Ala 85 634 24 PRT Conus purpurascens 634 Asp Cys Cys Gly Val Lys LeuGlu Met Cys His Pro Cys Leu Cys Asp 1 5 10 15 Asn Ser Cys Lys Asn TyrGly Lys 20 635 36 PRT Conus geographus 635 Gly Glu Pro Ile Pro Thr ThrVal Ile Asn Tyr Gly Glu Cys Cys Lys 1 5 10 15 Asp Pro Ser Cys Trp ValLys Val Lys Asp Phe Gln Cys Pro Gly Ala 20 25 30 Ser Pro Pro Asn 35 63616 PRT Conus generalis 636 Gln Cys Cys Thr Phe Cys Asn Phe Gly Cys GlnPro Cys Cys Val Pro 1 5 10 15 637 20 PRT Conus tessulatus 637 Asp GlyCys Pro Pro His Pro Val Pro Gly Met His Lys Cys Met Cys 1 5 10 15 ThrAsn Thr Cys 20 638 84 PRT Conus radiatus 638 His Pro Thr Lys Pro Cys MetTyr Cys Ser Phe Gly Gln Cys Val Gly 1 5 10 15 Pro His Ile Cys Cys GlyPro Thr Gly Cys Glu Met Gly Thr Ala Glu 20 25 30 Ala Asn Met Cys Ser GluGlu Asp Glu Asp Pro Ile Pro Cys Gln Val 35 40 45 Phe Gly Ser Asp Cys AlaLeu Asn Asn Pro Asp Asn Ile His Gly His 50 55 60 Cys Val Ala Asp Gly IleCys Cys Val Asp Asp Thr Cys Thr Thr His 65 70 75 80 Leu Gly Cys Leu

What is claimed is:
 1. An isolated peptide selected from the groupconsisting of: (a) a peptide set forth in Tables 1-14; and (b) aderivative of the peptide in (a).
 2. The isolated peptide of claim 1,wherein Xaa1 is Glu or γ-carboxy-Glu, Xaa2 is Gln or pyro-Glu, Xaa3 isPro or hydroxy-Pro, Xaa4 is Trp or bromo-Trp, and Xaa5 is Tyr, ¹²⁵I-Tyr,mono-iodo-Tyr, di-iodo-Tyr, O-sulpho-Tyr or O-phospho-Tyr.
 3. Thederivative of the peptide of claim 1, in which the Arg residues may besubstituted by Lys, omithine, homoargine, nor-Lys, N-methyl-Lys,N,N-dimethyl-Lys, N,N,N-trimethyl-Lys or any synthetic basic amino acid;the Lys residues may be substituted by Arg, omithine, homoargine,nor-Lys, or any synthetic basic amino acid; the Tyr residues may besubstituted with meta-Tyr, ortho-Tyr, nor-Tyr, mono-halo-Tyr,di-halo-Tyr, O-sulpho-Tyr, O-phospho-Tyr, nitro-Tyr or any synthetichydroxy containing amino acid; the Ser residues may be substituted withThr or any synthetic hydroxylated amino acid; the Thr residues may besubstituted with Ser or any synthetic hydroxylated amino acid; the Pheresidues may be substituted with any synthetic aromatic amino acid; theTrp residues may be substituted with Trp (D), neo-Trp, halo-Trp (D or L)or any aromatic synthetic amino acid; the Asn, Ser, Thr or Hyp residuesmay be glycosylated;. the Tyr residues may also be substituted with the3-hydroxyl or 2-hydroxyl isomers (meta-Tyr or ortho-Tyr, respectively)and corresponding O-sulpho- and O-phospho-derivatives; the acidic aminoacid residues may be substituted with any synthetic acidic amino acid,e.g., tetrazolyl derivatives of Gly and Ala; the aliphatic amino acidsmay be substituted by synthetic derivatives bearing non-naturalaliphatic branched or linear side chains C_(n)H_(2n+) ₂ up to andincluding n=8; the Leu residues may be substituted with Leu (D); the Gluresidues may be substituted with Gla; the Gla residues may besubstituted with Glu; the N-terminal Gln residues may be substitutedwith pyroGlu; the Met residues may be subsituted by Nle; the Cysresidues may be in D or L configuration and may optionally besubstituted with homocysteine (D or L); and pairs of Cys residues may bereplaced pairwise with isoteric lactam or ester-thioether replacements,such as Ser/(Glu or Asp), Lys/(Glu or Asp), Cys/(Glu or Asp) or Cys/Alacombinations.
 4. An isolated nucleic acid encoding an conotoxinpropeptide having an amino acid sequence set forth in Table
 1. 5. Theisolated nucleic acid of claim 4, wherein the nucleic acid comprises anucleotide sequence set forth in Table
 1. 6. An isolated conotoxinpropeptide having an amino acid sequence set forth in Table
 1. 7. Amethod of alleviating pain in an individual which comprisesadministering to said individual that is either exhibiting pain or isabout to be subjected to a pain-causing event a pain-alleviating amountof an active agent comprising a pain-relieving conotoxin peptide ofclaim 1 or a pharmaceutically acceptable salt thereof.
 8. A method fortreating or preventing disorders associated with a disorder selectedfrom the group consisting of voltage-gated ion channel disorders,ligand-gated ion channel disorders and receptor disorders in anindividual which comprises administering to an individual in needthereof a therapeutically effective amount of a conotoxin peptide ofclaim 1 or a pharmaceutically acceptable salt thereof.
 9. A method ofidentifying compounds that mimic the therapeutic activity of aconotoxin, comprising the steps of: (a) conducting a biological assay ona test compound to determine the therapeutic activity; and (b) comparingthe results obtained from the biological assay of the test compound tothe results obtained from the biological assay of a conotoxin.
 10. Asubstantially pure conotoxin peptide derivative comprising a permutantof the peptide of claim
 1. 11. A substantially pure conotoxin peptidederivative comprising a permutant of the peptide of claim
 2. 12. Use ofa radiolabeled conotoxin peptide of claim 1 for characterization of anew site on the aforementioned receptors or channels and use of thesepeptide probes for screening and identification of novel small moleculesthat interact at the aforementioned sites.
 13. The use of claim 12,wherein said receptor or channel is a monoamine transporter.
 14. The useof claim 13, wherein said peptide is selected from the group of peptidesset forth in Table 5.